Publication
A single‐synapse resolution survey of PSD95‐positive synapses in twenty human brain regions
Publisher:
Wiley
Date:
25-06-2020
DOI:
10.1111/EJN.14846
Abstract: Mapping the molecular composition of in idual excitatory synapses across the mouse brain reveals high synapse ersity with each brain region showing a distinct composition of synapse types. As a first step towards systematic mapping of synapse ersity across the human brain, we have labelled and imaged synapses expressing the excitatory synapse protein PSD95 in twenty human brain regions, including 13 neocortical, two subcortical, one hippoc al, one cerebellar and three brainstem regions, in four phenotypically normal in iduals. We quantified the number, size and intensity of in idual synaptic puncta and compared their regional distributions. We found that each region showed a distinct signature of synaptic puncta parameters. Comparison of brain regions showed that cortical and hippoc al structures are similar, and distinct from those of cerebellum and brainstem. Comparison of synapse parameters from human and mouse brain revealed conservation of parameters, hierarchical organization of brain regions and network architecture. This work illustrates the feasibility of generating a systematic single‐synapse resolution atlas of the human brain, a potentially significant resource in studies of brain health and disease.