ORCID Profile
0000-0003-2948-9471
Current Organisation
Universidade de Vigo Centro de Investigaciones Biomédicas
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Publisher: Elsevier BV
Date: 02-2010
Publisher: Springer Science and Business Media LLC
Date: 10-08-2017
Publisher: MDPI AG
Date: 09-02-2023
DOI: 10.3390/BIOS13020249
Abstract: The detection of pathogens in food substances is of crucial concern for public health and for the safety of the natural environment. Nanomaterials, with their high sensitivity and selectivity have an edge over conventional organic dyes in fluorescent-based detection methods. Advances in microfluidic technology in biosensors have taken place to meet the user criteria of sensitive, inexpensive, user-friendly, and quick detection. In this review, we have summarized the use of fluorescence-based nanomaterials and the latest research approaches towards integrated biosensors, including microsystems containing fluorescence-based detection, various model systems with nano materials, DNA probes, and antibodies. Paper-based lateral-flow test strips and microchips as well as the most-used trapping components are also reviewed, and the possibility of their performance in portable devices evaluated. We also present a current market-available portable system which was developed for food screening and highlight the future direction for the development of fluorescence-based systems for on-site detection and stratification of common foodborne pathogens.
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C4TB01882F
Abstract: Structural engineering of titania nanotubes (TNTs) with periodically shaped structures was successfully demonstrated as a strategy to improve drug loading and releasing performances of TNTs/Ti implants.
Publisher: MDPI AG
Date: 29-06-2018
DOI: 10.3390/BIOS8030062
Publisher: Elsevier BV
Date: 08-2020
Publisher: Elsevier BV
Date: 05-2017
DOI: 10.1016/J.BIOS.2017.01.018
Abstract: Molecularly imprinted polymers (MIPs) are biomimetics which can selectively bind to analytes of interest. One of the most interesting areas where MIPs have shown the biggest potential is food analysis. MIPs have found use as sorbents in s le preparation attributed to the high selectivity and high loading capacity. MIPs have been intensively employed in classical solid-phase extraction and solid-phase microextraction. More recently, MIPs have been combined with magnetic bead extraction, which greatly simplifies s le handling procedures. Studies have consistently shown that MIPs can effectively minimize complex food matrix effects, and improve recoveries and detection limits. In addition to s le preparation, MIPs have also been viewed as promising alternatives to bio-receptors due to the inherent molecular recognition abilities and the high stability in harsh chemical and physical conditions. MIPs have been utilized as receptors in biosensing platforms such as electrochemical, optical and mass biosensors to detect various analytes in food. In this review, we will discuss the current state-of-the-art of MIP synthesis and applications in the context of food analysis. We will highlight the imprinting methods which are applicable for imprinting food templates, summarize the recent progress in using MIPs for preparing and analysing food s les, and discuss the current limitations in the commercialisation of MIPs technology. Finally, future perspectives will be given.
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C3AN01933K
Publisher: American Chemical Society (ACS)
Date: 24-11-2010
DOI: 10.1021/AM100502U
Abstract: A method for surface engineering of structural gradients with nanopore topography using the self-ordering process based on electrochemical anodization of aluminum is described. A distinct anodization condition with an asymmetrically distributed electric field at the electrolyte/aluminum interface is created by nonparallel arrangement between electrodes (tilted by 45°) in an electrochemical cell. The anodic aluminum oxide (AAO) porous surfaces with ordered nanopore structures with gradual and continuous change of pore diameters from 80 to 300 nm across an area of 0.5-1 cm were fabricated by this anodization using two common electrolytes, oxalic acid (0.3 M) and phosphoric acid (0.3 M). The formation of pore gradients of AAO is explained by asymmetric and gradual distribution of the current density and temperature variation generated on the surface of Al during the anodization process. Optical and wetting gradients of prepared pore structures were confirmed by reflective interferometric spectroscopy and contact angle measurements showing the ability of this method to generate porous surfaces with multifunctional gradients (structural, optical, wetting). The study of influence of pore structures on cell growth using the culture of neuroblastoma cells reveals biological relevance of nanopore gradients and the potential to be applied as the platform for spatially controllable cell growth and cell differentiation.
Publisher: Elsevier BV
Date: 02-2019
Publisher: Elsevier BV
Date: 05-2017
DOI: 10.1016/J.BIOS.2017.01.018
Abstract: Molecularly imprinted polymers (MIPs) are biomimetics which can selectively bind to analytes of interest. One of the most interesting areas where MIPs have shown the biggest potential is food analysis. MIPs have found use as sorbents in s le preparation attributed to the high selectivity and high loading capacity. MIPs have been intensively employed in classical solid-phase extraction and solid-phase microextraction. More recently, MIPs have been combined with magnetic bead extraction, which greatly simplifies s le handling procedures. Studies have consistently shown that MIPs can effectively minimize complex food matrix effects, and improve recoveries and detection limits. In addition to s le preparation, MIPs have also been viewed as promising alternatives to bio-receptors due to the inherent molecular recognition abilities and the high stability in harsh chemical and physical conditions. MIPs have been utilized as receptors in biosensing platforms such as electrochemical, optical and mass biosensors to detect various analytes in food. In this review, we will discuss the current state-of-the-art of MIP synthesis and applications in the context of food analysis. We will highlight the imprinting methods which are applicable for imprinting food templates, summarize the recent progress in using MIPs for preparing and analysing food s les, and discuss the current limitations in the commercialisation of MIPs technology. Finally, future perspectives will be given.
Publisher: Hindawi Limited
Date: 2016
DOI: 10.1155/2016/4350952
Publisher: American Institute of Physics
Date: 2009
DOI: 10.1063/1.3203224
Publisher: Elsevier BV
Date: 03-2019
DOI: 10.1016/J.BIOS.2018.09.078
Abstract: Foodborne salmonellosis remains a major economic burden worldwide and particularly for food industries. The erse and complexity of food matrices pose great challenges for rapid and ultra-sensitive detection of Salmonella in food s les. In this study, combination of pathogen pre-concentration with rapid molecular identification is presented to overcome these challenges. This combination enabled effective real-time PCR detection of low levels of Salmonella enterica serovar Typhimurium without culture enrichment. Anti-salmonella antibody, immobilized on protein AG-magnetic beads, could efficiently concentrate Salmonella Typhimurium with a capturing efficiency of 95%. In the direct PCR, a strong linear relationship between bacteria concentration and the number of cycles was observed with a relative PCR efficiency of ∼92% resulting in a limit of detection (LoD) of ∼2 CFU/mL. Analysis of spiked food s les that include vegetable salad, egg yolk, egg white, whole egg and minced pork meat has validated the precision of the method. A relative accuracy of 98.3% with a sensitivity of 91.6% and specificity of 100% was achieved in the Salmonella spiked food s les. The use of a Phusion hot start DNA polymerase with a high tolerance to possible PCR inhibitors allowed the integration of direct PCR, and thereby reducing the duration of analysis to less than 3 h. The Cohen's kappa index showed excellent agreement (0.88) signifying the capability of this method to overcome the food matrix effects in rapid and ultra-sensitive detection of Salmonella in food. This approach may lay a future platform for the integration into a Lab-on-a-chip system for online monitoring of foodborne pathogens.
Publisher: Elsevier BV
Date: 09-2014
Publisher: Royal Society of Chemistry (RSC)
Date: 22-07-2014
DOI: 10.1039/C4CC04470C
Abstract: In the quest for solutions to meeting future energy demands, solar fuels play an important role. A particularly promising ex le is photocatalysis since even incremental improvements in performance in this process are bound to translate into significant cost benefits. Here, we report that semiconducting and high surface area 3D silicon replicas prepared from abundantly available diatom fossils sustain photocurrents and enable solar energy conversion.
Publisher: MDPI AG
Date: 18-03-2021
Abstract: Currently, conventional pre-clinical in vitro studies are primarily based on two-dimensional (2D) cell culture models, which are usually limited in mimicking the real three-dimensional (3D) physiological conditions, cell heterogeneity, cell to cell interaction, and extracellular matrix (ECM) present in living tissues. Traditionally, animal models are used to mimic the 3D environment of tissues and organs, but they suffer from high costs, are time consuming, bring up ethical concerns, and still present many differences when compared to the human body. The applications of microfluidic-based 3D cell culture models are advantageous and useful as they include 3D multicellular model systems (MCMS). These models have demonstrated potential to simulate the in vivo 3D microenvironment with relatively low cost and high throughput. The incorporation of monitoring capabilities in the MCMS has also been explored to evaluate in real time biophysical and chemical parameters of the system, for ex le temperature, oxygen, pH, and metabolites. Electrochemical sensing is considered as one of the most sensitive and commercially adapted technologies for bio-sensing applications. Amalgamation of electrochemical biosensing with cell culture in microfluidic devices with improved sensitivity and performance are the future of 3D systems. Particularly in cancer, such models with integrated sensing capabilities can be crucial to assess the multiple parameters involved in tumour formation, proliferation, and invasion. In this review, we are focusing on existing 3D cell culture systems with integrated electrochemical sensing for potential applications in cancer models to advance diagnosis and treatment. We discuss their design, sensing principle, and application in the biomedical area to understand the potential relevance of miniaturized electrochemical hybrid systems for the next generation of diagnostic platforms for precision medicine.
Publisher: MDPI AG
Date: 11-11-2014
DOI: 10.3390/S141121316
Publisher: American Scientific Publishers
Date: 07-2014
Publisher: MDPI AG
Date: 17-07-2023
DOI: 10.3390/BIOS13070737
Abstract: Drug delivery systems (DDS) are continuously being explored since humans are facing more numerous complicated diseases than ever before. These systems can preserve the drug’s functionality and improve its efficacy until the drug is delivered to a specific site within the body. One of the least used materials for this purpose are metal—organic frameworks (MOFs). MOFs possess many properties, including their high surface area and the possibility for the addition of functional surface moieties, that make them ideal drug delivery vehicles. Such properties can be further improved by combining different materials (such as metals or ligands) and utilizing various synthesis techniques. In this work, the microfluidic technique is used to synthesize Zeolitic Imidazole Framework-67 (ZIF-67) containing cobalt ions as well as its bimetallic variant with cobalt and zinc as ZnZIF-67 to be subsequently loaded with diclofenac sodium and incorporated into sodium alginate beads for sustained drug delivery. This study shows the utilization of a microfluidic approach to synthesize MOF variants. Furthermore, these MOFs were incorporated into a biopolymer (sodium alginate) to produce a reliable DDS which can perform sustained drug releases for up to 6 days (for 90% of the full amount released), whereas MOFs without the biopolymer showed sudden release within the first day.
Publisher: Wiley
Date: 2022
Publisher: Wiley
Date: 07-2009
Publisher: American Chemical Society (ACS)
Date: 31-03-2021
Publisher: Elsevier BV
Date: 2020
Publisher: Bio-Protocol, LLC
Date: 2019
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6RA06701H
Abstract: The ionic conductivity and electrochemical properties of vertically aligned CNT composite membranes produced by template-based catalyst-free chemical vapor deposition is tuned by chemical modification of their inner surfaces using simple oxidation.
Publisher: MDPI AG
Date: 02-04-2019
DOI: 10.3390/APP9071387
Abstract: Surface-enhanced Raman scattering (SERS) spectroscopy stands out due to its sensitivity, selectivity, and multiplex ability. The development of ready-to-use, simple, and low-cost SERS substrates is one of the main challenges of the field. In this paper, the intrinsic reproducibility of microfluidics technology was used for the fabrication of self-assembled nanoparticle structures over a paper film. The paper SERS substrates were fabricated by assembling anisotropic particles, gold nanostars (GNSs), and nanorods (NRs) onto paper to offer an extra enhancement to reach ultra-sensitive detection limits. A polydimethylsiloxane PDMS-paper hybrid device was used to control the drying kinetics of the nanoparticles over the paper substrate. This method allowed a high reproducibility and homogeneity of the fabrication of SERS substrates that reach limits of detection down to the picomolar range. This simple and low-cost fabrication of a paper-based sensing device was tested for the discrimination of different cell lineages.
Publisher: Elsevier BV
Date: 2010
DOI: 10.1016/J.BIOMATERIALS.2009.09.074
Abstract: Nanotubular titanium oxide (TiO(2)) produced by self-ordering processes using electrochemical anodization have been extensively explored in recent years as a new biomaterial for implants, drug delivery systems, cell growth, biosensors, immunoisolations, bioartificial organs and tissue engineering. Chemical inertness is the main weakness of this material when placed in contact with biological systems and surface modification is a possible solution of this problem. The aim of this study is to develop a flexible and facile method for surface modification of TiO(2) nanotubes to tailor new interfacial properties important in many biomedical applications. TiO(2) nanotubes were prepared by electrochemical anodization of titanium foil using ethylene glycol: NH(4)F electrolyte (2% water and 0.3% NH(4)F). Plasma surface modification using allylamine (AA) as a precursor has been applied to generate a thin and chemically reactive polymer (AAPP) film rich in amine groups on top of the TiO(2) nanotube surface. This initial polymer film was used for further surface functionalization by attachment of desired molecules. Two modification techniques were used to demonstrate the flexibility for building of new functionalities on titania nanotube surface: electrostatic adsorption of poly(sodium styrenesulfonate) (PSS) as an ex le of layer-by-layer assembly (LbL), and covalent coupling of poly(ethylene glycol) (PEG) as an ex le of creating a protein-resistant surface. These approaches for tailoring the surface chemistry and wettability of TiO(2) nanotubes offer considerable prospects for advancing their interfacial properties to improve existing and develop new functional biomaterials for erse biomedical applications.
Publisher: CSIRO Publishing
Date: 2011
DOI: 10.1071/CH10398
Abstract: In this paper, we present recent work from our group focussed on the fabrication of nanopore and nanotube arrays using self-ordered electrochemistry, and their application in several key areas including template synthesis, molecular separation, optical sensing, and drug delivery. We have fabricated nanoporous anodic aluminium oxide (AAO) with controlled pore dimensions (20–200 nm) and shapes, and used them as templates for the preparation of gold nanorod/nanotube arrays and gold nanotube membranes with characteristic properties such as surface enhanced Raman scattering and selective molecular transport. The application of AAO nanopores as a sensing platform for reflective interferometric detection is demonstrated. Finally, a drug release study on fabricated titania nanotubes confirms their potential for implantable drug delivery applications.
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6RA06701H
Abstract: The ionic conductivity and electrochemical properties of vertically aligned CNT composite membranes produced by template-based catalyst-free chemical vapor deposition is tuned by chemical modification of their inner surfaces using simple oxidation.
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5RA01336D
Abstract: This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction.
Publisher: MDPI AG
Date: 07-04-2022
DOI: 10.3390/BIOS12040220
Abstract: CTCs (circulating tumor cells) are well-known for their use in clinical trials for tumor diagnosis. Capturing and isolating these CTCs from whole blood s les has enormous benefits in cancer diagnosis and treatment. In general, various approaches are being used to separate malignant cells, including immunomagnets, macroscale filters, centrifuges, dielectrophoresis, and immunological approaches. These procedures, on the other hand, are time-consuming and necessitate multiple high-level operational protocols. In addition, considering their low efficiency and throughput, the processes of capturing and isolating CTCs face tremendous challenges. Meanwhile, recent advances in microfluidic devices promise unprecedented advantages for capturing and isolating CTCs with greater efficiency, sensitivity, selectivity and accuracy. In this regard, this review article focuses primarily on the various fabrication methodologies involved in microfluidic devices and techniques specifically used to capture and isolate CTCs using various physical and biological methods as well as their conceptual ideas, advantages and disadvantages.
Publisher: Oxford University Press (OUP)
Date: 21-11-2017
Publisher: Wiley
Date: 04-12-2021
Abstract: The internal surfaces of all blood and lymphatic vessels are lined with an endothelium, which tightly controls and regulates the permeability of biological molecules. A dysfunctional endothelium (Dys‐En) is a hallmark of many diseases, including atherosclerosis. Dys‐En in atherosclerosis leads to loss of adherens junctions between cells, thus enhancing permeability and upregulation of adhesion receptors such as vascular cell adhesion molecule 1 (VCAM‐1). Both this enhanced permeability of the endothelium and associated upregulated endothelial cell surface receptors can be exploited in nanomedicine targeting to atherosclerotic plaques. However, the relationship between targeting ligand and nanoparticle (NP) size is not well understood within this context. Herein, a biomicrofluidic model of Dys‐En is developed and this platform is used to screen VCAM‐1 targeted NPs. Screening of NPs with varying properties under flow shows that size plays a dominant role in NP targeting, with NPs in the range of 30–60 nm showing increased targeting to Dys‐En. Moreover, treatment of Dys‐En‐on‐a‐chip with Annexin A1, as a novel proresolving mediator of inflammation, results in restoration of adherens junctions and normalization of the barrier integrity. The results demonstrate utility of using “Dys‐En‐on‐a‐chip” as a screening platform for Dys‐En‐targeted nanomedicines and biologics.
Publisher: Elsevier BV
Date: 04-2019
DOI: 10.1038/S41374-018-0143-3
Abstract: The volume of point of care (POC) testing continues to grow steadily due to the increased availability of easy-to-use devices, thus making it possible to deliver less costly care closer to the patient site in a shorter time relative to the central laboratory services. A novel class of molecules called microRNAs have recently gained attention in healthcare management for their potential as biomarkers for human diseases. The increasing interest of miRNAs in clinical practice has led to an unmet need for assays that can rapidly and accurately measure miRNAs at the POC. However, the most widely used methods for analyzing miRNAs, including Northern blot-based platforms, in situ hybridization, reverse transcription qPCR, microarray, and next-generation sequencing, are still far from being used as ideal POC diagnostic tools, due to considerable time, expertize required for s le preparation, and in terms of miniaturizations making them suitable platforms for centralized labs. In this review, we highlight various existing and upcoming technologies for miRNA lification and detection with a particular emphasis on the POC testing industries. The review summarizes different miRNA targets and signals lification-based assays, from conventional methods to alternative technologies, such as isothermal lification, paper-based, oligonucleotide-templated reaction, nanobead-based, electrochemical signaling- based, and microfluidic chip-based strategies. Based on critical analysis of these technologies, the possibilities and feasibilities for further development of POC testing for miRNA diagnostics are addressed and discussed.
Publisher: American Association for the Advancement of Science (AAAS)
Date: 03-2019
Abstract: The binary neutron star merger event GW170817 was observed with gravitational waves and across the electromagnetic spectrum. However, the physical processes that produced that emission remain poorly understood, particularly the late-time x-ray and radio emission. Ghirlanda et al. observed the radio afterglow with an interferometric array of 32 radio telescopes spread across the globe. The size and position of the radio source are not compatible with a uniformly expanding cocoon, as some have suggested. Instead, the data indicate that GW170817 produced a structured jet of material that escaped the surrounding ejecta and is now expanding into the interstellar medium at relativistic speeds. Science , this issue p. 968
Publisher: Springer International Publishing
Date: 2013
Publisher: Elsevier BV
Date: 07-2018
DOI: 10.1016/J.BIOTECHADV.2018.03.002
Abstract: Rapid detection of foodborne pathogens at an early stage is imperative for preventing the outbreak of foodborne diseases, known as serious threats to human health. Conventional bacterial culturing methods for foodborne pathogen detection are time consuming, laborious, and with poor pathogen diagnosis competences. This has prompted researchers to call the current status of detection approaches into question and leverage new technologies for superior pathogen sensing outcomes. Novel strategies mainly rely on incorporating all the steps from s le preparation to detection in miniaturized devices for online monitoring of pathogens with high accuracy and sensitivity in a time-saving and cost effective manner. Lab on chip is a blooming area in diagnosis, which exploits different mechanical and biological techniques to detect very low concentrations of pathogens in food s les. This is achieved through streamlining the s le handling and concentrating procedures, which will subsequently reduce human errors and enhance the accuracy of the sensing methods. Integration of s le preparation techniques into these devices can effectively minimize the impact of complex food matrix on pathogen diagnosis and improve the limit of detections. Integration of pathogen capturing bio-receptors on microfluidic devices is a crucial step, which can facilitate recognition abilities in harsh chemical and physical conditions, offering a great commercial benefit to the food-manufacturing sector. This article reviews recent advances in current state-of-the-art of s le preparation and concentration from food matrices with focus on bacterial capturing methods and sensing technologies, along with their advantages and limitations when integrated into microfluidic devices for online rapid detection of pathogens in foods and food production line.
Publisher: MDPI AG
Date: 24-08-2023
DOI: 10.3390/BIOS13090843
Abstract: Biosensors are a promising tool for a wide variety of target analyte detection and enable point-of-care diagnostics with reduced volume and space [...]
Publisher: Elsevier BV
Date: 09-2014
Publisher: MDPI AG
Date: 07-02-2022
DOI: 10.3390/S22031259
Abstract: Plant diseases and their diagnoses are currently one of the global challenges and causes significant impact to the economy of farmers and industries depending on plant-based products. Plant pathogens such as viruses, bacteria, fungi, and pollution caused by the nanomaterial, as well other important elements of pollution, are the main reason for the loss of plants in agriculture and in forest ecosystems. Presently, various techniques are used to detect pathogens in trees, which includes DNA-based techniques, as well as other microscopy based identification and detection. However, these methodologies require complex instruments and time. Lately, nanomaterial-based new biosensing systems for early detection of diseases, with specificity and sensitivity, are developed and applied. This review highlights the nanomaterial-based biosensing methods of disease detection. Precise and time effective identification of plant pathogens will help to reduce losses in agriculture and forestry. This review focuses on various plant diseases and the requirements for a reliable, fast, and cost-effective testing method, as well as new biosensing technologies for the detection of diseases of field plants in forests at early stages of their growth.
Publisher: World Scientific Pub Co Pte Lt
Date: 02-2011
DOI: 10.1142/S0219581X11007454
Abstract: We report the fabrication of highly ordered TiO 2 nanotube arrays employing electrochemical anodization of titanium using an organic electrolyte comprised of water, NH 4 F , and ethylene glycol. To achieve the self-ordering regime of TiO 2 nanotube growth and reliable fabrication optimal potential window between 80 and 100 V was determined. We show that anodization voltage can be used not only to control nanotube diameters (70–180 nm) but also to have impact on nanotube growth rate. The anodization voltage and anodization time were used to adjust the length of TiO 2 nanotube (thickness of nanotube layer). TiO 2 nanotube array films and self-supporting layers with thickness from 5 μm to 250 μm were routinely fabricated.
Publisher: American Chemical Society (ACS)
Date: 28-03-2019
Publisher: CRC Press
Date: 11-10-2022
Publisher: IEEE
Date: 2017
Publisher: MDPI AG
Date: 29-06-2018
DOI: 10.3390/BIOS8030062
Publisher: Elsevier BV
Date: 05-2012
Publisher: American Chemical Society (ACS)
Date: 27-12-2018
Publisher: World Scientific Pub Co Pte Lt
Date: 02-2011
DOI: 10.1142/S0219581X11007466
Abstract: This work presents template synthesis of ordered arrays of nickel, cobalt and nickel hexacyanoferrates ( NiHCF ) with several distinct morphologies such as dots, rods, and tubes. Anodic alumina oxide (AAO) with preferred pore diameters and thickness was fabricated by electrochemical anodization of aluminum used as template. Ni and Co nanostructures inside AAO template were prepared by electrodeposition using galvanostatic method. NiHCF nanostructures were prepared by electrochemical oxidation of Ni using cyclic voltametry (CV) in the presence of hexacyanoferrate ions. The morphology, chemical, and functional properties of prepared nanostructures were investigated by scanning electron microscopy (SEM), energy-dispersive X-ray microscopy (EDAX), and electrochemical methods. The electrocatalytic properties of NiHCF nanorod arrays electrode and their potential for the detection of hydrogen peroxide and biosensing application were demonstrated.
Location: Spain
Start Date: 2019
End Date: 2019
Funder: Gesellschaft zur Förderung der Lebenswissenschaften Heidelberg GmbH
View Funded ActivityStart Date: 2021
End Date: 2023
Funder: European Commission
View Funded ActivityStart Date: 2019
End Date: 2019
Funder: European Molecular Biology Organization
View Funded Activity