ORCID Profile
0000-0002-0904-3862
Current Organisation
University of Oxford
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Publisher: Elsevier BV
Date: 09-2017
Publisher: SPIE
Date: 14-07-2015
DOI: 10.1117/12.2183843
Publisher: Elsevier BV
Date: 07-2020
Publisher: Springer Science and Business Media LLC
Date: 19-10-2015
Publisher: Frontiers Media SA
Date: 08-10-2014
Publisher: SPIE
Date: 05-03-2015
DOI: 10.1117/12.2087342
Publisher: SPIE
Date: 09-03-2015
DOI: 10.1117/12.2076133
Publisher: Ovid Technologies (Wolters Kluwer Health)
Date: 13-09-2013
DOI: 10.1161/CIRCRESAHA.113.301704
Abstract: Sarcomere length (SL) is a key indicator of cardiac mechanical function, but current imaging technologies are limited in their ability to unambiguously measure and characterize SL at the cell level in intact, living tissue. We developed a method for measuring SL and regional cell orientation using remote focusing microscopy, an emerging imaging modality that can capture light from arbitrary oblique planes within a s le. We present a protocol that unambiguously and quickly determines cell orientation from user-selected areas in a field of view by imaging 2 oblique planes that share a common major axis with the cell. We demonstrate the effectiveness of the technique in establishing single-cell SL in Langendorff-perfused hearts loaded with the membrane dye di-4-ANEPPS. Remote focusing microscopy can measure cell orientation in complex 2-photon data sets without capturing full z stacks. The technique allows rapid assessment of SL in healthy and diseased heart experimental preparations.
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Rebecca-Ann Burton.