ORCID Profile
0000-0001-7820-4271
Current Organisation
Hong Kong University of Science and Technology
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Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D1SC06336G
Abstract: A novel bioconjugatable and photostable AIE luminogen has been rationally synthesized for precise and long-term tracking of neuron mitochondria.
Publisher: American Chemical Society (ACS)
Date: 16-12-2021
Publisher: American Chemical Society (ACS)
Date: 06-04-2022
DOI: 10.1021/ACS.ANALCHEM.2C00067
Abstract: High sensitivity and specificity nucleic acid detection has been achieved by the Cas13a collateral effect in combination with a separate recombinase polymerase lification (RPA). However, these emerging methods cannot provide accurate quantification of nucleic acids because the two-step assay performance may be compromised if the RPA and Cas13a reactions are simply unified in a single step. In this work, we first addressed the challenges associated with enzymatic incompatibility and the macromolecular crowding effect in the one-pot assay development, making the consolidated RPA-Cas13a assay a facile and robust diagnostic tool. Next, we found that the one-pot reaction cannot precisely quantify the targets at low concentrations. Thus, by leveraging droplet microfluidics, we converted the one-pot assay to a digital quantification format, termed Microfluidics-Enabled Digital Isothermal Cas13a Assay (MEDICA). Due to the droplet compartmentation, MEDICA greatly accelerates the reaction and enables relative detection in 10 min and the end-point quantification in 25 min. Moreover, MEDICA facilitates the droplet binarization for counting because of background-free signals generated by trans-cleavage reporting of Cas13a. Our clinical validation highlights that CRISPR-based isothermal assays are promising for the next generation of nucleic acid quantification methods.
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D1QM01495A
Abstract: TPEPy-Ala and TPAPy-Kdo with metabolic moieties can be directly incorporated into the bacterial cell envelopes and light up intracellular bacteria. Additionally, the metabolic probes can effectively eliminate labeled bacteria in situ with minimal host cell cytotoxicity via photodyanmic therapy.
Publisher: Wiley
Date: 07-06-2022
DOI: 10.1002/AGT2.239
Abstract: Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical research. Despite all the efforts, scientists lack a proper strategy to achieve high selectivity for primary amines, not to mention the requirement of fast response in real applications. Herein, we report a chromone‐based aggregation‐induced emission (AIE) fluorogen called CMVMN as a self‐reporting bioconjugation reagent for selective primary amine identification, and its applications for monitoring bioprocesses of amination and protein labeling. CMVMN is AIE‐active and capable of solid‐state sensing. Thus, its electrospun films are manufactured for visualization of amine diffusion and leakage process. CMVMN also shows good biocompatibility and potential mitochondria‐staining ability, which provides new insight for organelle‐staining probe design. Combined with its facile synthesis and good reversibility, CMVMN would not only show wide potential applications in biology, but also offer new possibilities for molecular engineering.
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D0QM00877J
Abstract: We present a two-photon AIEgen, ABCXF with nonaromatic rotors (–CF 3 ), a high two-photon absorption cross-section, bright red emission in the solid-state, and remarkably high photostability for fatty liver disease diagnosis.
No related grants have been discovered for Hojeong Park.