ORCID Profile
0000-0003-4662-4348
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Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1EN00021G
Abstract: AgNP toxicity was attributed to dissolved Ag + , which was released, transported, and concentrated in the mitochondria, finally leading to exhaustion of the reserve respiratory capacity and cell death.
Publisher: American Chemical Society (ACS)
Date: 22-06-2021
Abstract: Nanoparticles (NPs) for delivering chemotherapeutic drugs are now in clinical trials, and cellular uptake of NPs plays an important role in determining the drug delivery efficiency. Herein, we reported that the bioaccumulation and internalization of NPs were governed by the cell cycle. Specifically, we found that the bioaccumulation of NPs was more favored in the G 2 /M stages, followed by the S and G 0 /G 1 stages. We demonstrated that three key parameters-clathrin-mediated endocytosis capacity, algal cell membrane permeability, and exopolymer substance (EPS) thickness-were critical in the bioaccumulation of NPs during the cell cycling process. Over the 24-h average duration of cell cycle, clathrin-mediated endocytosis capacity was much higher at the S stage than that at the G 0 /G 1 and G 2 /M stages. Besides, cell membrane permeability was measured to be higher in S and G 2 /M stages while the lowest in G 0 /G 1 stage. We have also identified the change of EPS thickness during the 24-h cell cycle. Transition from G 0 /G 1 to S and G 2 /M induced the attenuation in EPS thickness, and the thinnest EPS was found at the end of mitosis. The cell cycle control NPs internalization were further verified by exposing Ag nanoparticles to algae at different cell cycle stages, confirming the important roles of EPS thickness and cell cycle control in the dynamic internalization processes. The present study highlights the important roles of cell cycle controlling the NPs bioaccumulation and internalization, with possible implications in maximizing NPs internalization efficiency while reducing the cost.
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/C9SC06226B
Abstract: An AIE-active ratiometric probe for the first time achieved the long-term quantification of lysosomal pH during the medaka larva's caudal fin regeneration.
Publisher: American Chemical Society (ACS)
Date: 13-09-2022
Abstract: Microalgae-based biofuels are receiving attention at the environmental, economic, and social levels because they are clean, renewable, and quickly produced. The green algae
Publisher: Wiley
Date: 29-05-2019
Publisher: American Chemical Society (ACS)
Date: 29-04-2019
Abstract: Although numerous studies have been conducted on the toxicity and biodistribution of AgNPs and corresponding ionic counterparts, it is still debatable whether the toxicity originates from the accumulation of particles within specific organs or is mediated by the dissolved Ag ions. To gain a better insight into the toxic mechanisms of AgNPs, two aggregation-induced emission fluorogens (AIEgens AIEgens-coated AgNPs and a fluorogenic Ag
Publisher: American Chemical Society (ACS)
Date: 20-11-2018
Abstract: Release of silver ions (Ag
Publisher: American Chemical Society (ACS)
Date: 23-06-2022
DOI: 10.1021/JACS.2C04032
Abstract: The development of straightforward and efficient synthetic methods toward ring-fused heteroaromatic polymers with attractive functionalities has great significance in both chemistry and materials science. Herein, we develop a facile cascade C-H-activated polyannulation route that can in situ generate multiple ring-fused aza-heteroaromatic polymers from readily available monomers in an atom-economical manner. A series of complex polybenzimidazole derivatives with high absolute molecular weights of up to 24 000 are efficiently produced in high yields within 2 h. Benefiting from their unique imidazole-containing ring-fused structures with multiple aryl pendants, the obtained polymers show excellent thermal and morphological stability, good solution processability, high refractive index, small chromic dispersion, as well as remarkable acid-base-responsive fluorescence. Taking advantage of the ratiometric fluorescence response of the triphenylamine-substituted heteroaromatic polymer to pH variations, we successfully apply it as a sensitive fluorescence probe for the mapping and quantitative analysis of intracellular pH in live cells. Furthermore, through the simple
Publisher: American Chemical Society (ACS)
Date: 03-11-2021
Publisher: American Chemical Society (ACS)
Date: 05-11-2021
DOI: 10.1021/ACSSENSORS.1C01821
Abstract: Extracellular polymeric substances (EPS) are produced by many microorganisms and play an essential role in physiological systems such as nutrient storage and stress resistance. Besides, EPS show great potential in biomedical and therapeutic applications due to their biocompatibility and biodegradability. In situ noninvasive monitoring of the EPS produced by microorganisms is thus critical but has not yet been achieved. Herein, we developed a novel aggregation-induced emission (AIE) active nanoprobe enabling in situ visualization of the EPS distribution produced by various microorganisms (cyanobacteria, yeast, freshwater, and marine phytoplankton). The synthesized AIE-active nanoprobe displayed excellent specificity and precision for the staining of EPS, as well as strong photostability, showing great advantage in sensing the EPS in living organisms. With the application of this novel probe, the three-dimensional (3D) framework of EPS distribution was visualized under different environmental conditions (temperature, light intensity, nutrition, and pH). The EPS distribution was found to correlate significantly with the metal tolerance and cyanobacterial photosynthesis capability. Collectively, this study proposed an AIE-active nanoprobe for visualizing the EPS distribution and quantifying the EPS thickness/volume, and has significant implications in understanding the physiological functions of microorganisms.
Publisher: American Chemical Society (ACS)
Date: 31-07-2020
No related grants have been discovered for Neng YAN.