ORCID Profile
0000-0002-7265-2804
Current Organisations
University of California, San Diego
,
RIKEN SPring-8 Center
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Publications
Phosphatidylglycerol is involved in the dimerization of photosystem II
Publisher: Elsevier BV
Date: 03-2000
The pea branching RMS2 gene encodes the PsAFB4/5 auxin receptor and is involved in an auxin-strigolactone regulation loop
Publisher: Public Library of Science (PLoS)
Date: 08-12-2017
Three-dimensional Structure of the Photosystem II Core Dimer of Higher Plants Determined by Electron Microscopy
Publisher: Elsevier BV
Date: 09-2001
Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry
Publisher: American Association for the Advancement of Science (AAAS)
Date: 16-11-2018
Abstract: Insights into the architecture and stoichiometry of membrane complexes have grown with advances in cryo–electron microscopy and native mass spectroscopy. However, most of these studies are not in the context of native membrane. Chorev et al. released intact membrane complexes directly from native lipid membrane vesicles into a mass spectrometer. They analyzed components of the Escherichia coli inner and outer membranes and the bovine mitochondrial inner membrane. For several identified complexes, they found a stoichiometry that differs from published results and, in some cases, confirmed interactions that could not be characterized structurally. Science , this issue p. 829
Cooperative defence operates by social modulation of biogenic amine levels in the honey bee brain
Publisher: The Royal Society
Date: 24-01-2018
Abstract: The defence of a society often requires that some specialized members coordinate to repel a threat at personal risk. This is especially true for honey bee guards, which defend the hive and may sacrifice their lives upon stinging. Central to this cooperative defensive response is the sting alarm pheromone, which has isoamyl acetate (IAA) as its main component. Although this defensive behaviour has been well described, the neural mechanisms triggered by IAA to coordinate stinging have long remained unknown. Here we show that IAA upregulates brain levels of serotonin and dopamine, thereby increasing the likelihood of an in idual bee to attack and sting. Pharmacological enhancement of the levels of both amines induces higher defensive responsiveness, while decreasing them via antagonists decreases stinging. Our results thus uncover the neural mechanism by which an alarm pheromone recruits in iduals to attack and repel a threat, and suggest that the alarm pheromone of honey bees acts on their response threshold rather than as a direct trigger.
RAZA: A Rapid 3D z-crossings algorithm to segment electron tomograms and extract organelles and macromolecules
Publisher: Elsevier BV
Date: 11-2017
DOI: 10.1016/J.JSB.2017.10.002
Abstract: Resolving the 3D architecture of cells to atomic resolution is one of the most ambitious challenges of cellular and structural biology. Central to this process is the ability to automate tomogram segmentation to identify sub-cellular components, facilitate molecular docking and annotate detected objects with associated metadata. Here we demonstrate that RAZA (Rapid 3D z-crossings algorithm) provides a robust, accurate, intuitive, fast, and generally applicable segmentation algorithm capable of detecting organelles, membranes, macromolecular assemblies and extrinsic membrane protein domains. RAZA defines each continuous contour within a tomogram as a discrete object and extracts a set of 3D structural fingerprints (major, middle and minor axes, surface area and volume), enabling selective, semi-automated segmentation and object extraction. RAZA takes advantage of the fact that the underlying algorithm is a true 3D edge detector, allowing the axes of a detected object to be defined, independent of its random orientation within a cellular tomogram. The selectivity of object segmentation and extraction can be controlled by specifying a user-defined detection tolerance threshold for each fingerprint parameter, within which segmented objects must fall and/or by altering the number of search parameters, to define morphologically similar structures. We demonstrate the capability of RAZA to selectively extract subgroups of organelles (mitochondria) and macromolecular assemblies (ribosomes) from cellular tomograms. Furthermore, the ability of RAZA to define objects and their contours, provides a basis for molecular docking and rapid tomogram annotation.
Related Organisations
University Of California, San Diego
Location: United States of America
University Of Minnesota Twin Cities
Location: United States of America
Gottfried Wilhelm Leibniz Universität Hannover
Location: No location found
Imperial College London
Location: United Kingdom of Great Britain and Northern Ireland
Related Funding Activities
No related grants have been discovered for Christoph Gerle.