ORCID Profile
0000-0001-7895-3563
Current Organisation
Empa
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Publisher: Wiley
Date: 15-03-2018
Abstract: 3D human skin models provide a platform for toxicity testing, biomaterials evaluation, and investigation of fundamental biological processes. However, the majority of current in vitro models lack an inflammatory system, vasculature, and other characteristics of native skin, indicating scope for more physiologically complex models. Looking at the immune system, there are a variety of cells that could be integrated to create novel skin models, but to do this effectively it is also necessary to understand the interface between skin biology and tissue engineering as well as the different roles the immune system plays in specific health and disease states. Here, a progress report on skin immunity and current immunocompetent skin models with a focus on construction methods is presented scaffold and cell choice as well as the requirements of physiologically relevant models are elaborated. The wide range of technological and fundamental challenges that need to be addressed to successfully generate immunocompetent skin models and the steps currently being made globally by researchers as they develop new models are explored. Induced pluripotent stem cells, microfluidic platforms to control the model environment, and new real-time monitoring techniques capable of probing biochemical processes within the models are discussed.
Publisher: American Vacuum Society
Date: 12-2017
DOI: 10.1116/1.5018515
Publisher: American Vacuum Society
Date: 12-2018
DOI: 10.1116/1.5082176
Publisher: Elsevier BV
Date: 11-2014
DOI: 10.1016/J.BIOMATERIALS.2014.07.015
Abstract: Tissue engineering using scaffold-cell constructs holds the potential to develop functional strategies to regenerate bone. The interface of orthopedic implants with the host tissues is of great importance for its later performance. Thus, the optimization of the implant surface in a way that could stimulate osteogenic differentiation of mesenchymal stem cells (MSCs) is of significant therapeutic interest. The effect of surface roughness of polycaprolactone (PCL) on the osteogenic differentiation of human bone-marrow MSCs was investigated. We prepared surface roughness gradients of average roughness (Ra) varying from the sub-micron to the micrometer range (∼0.5-4.7 μm), and mean distance between peaks (RSm) gradually varying from ∼214 μm to 33 μm. We analyzed the degree of cytoskeleton spreading, expression of alkaline phosphatase, collagen type 1 and mineralization. The response of cells to roughness ided the gradient into three groups of elicited stem cell behavior: 1) faster osteogenic commitment and strongest osteogenic expression 2) slower osteogenic commitment but strong osteogenic expression, and 3) similar or inferior osteogenic potential in comparison to the control material. The stem-cell modulation by specific PCL roughness surfaces highlights the potential for creating effective solutions for orthopedic applications featuring a clinically relevant biodegradable material.
Publisher: American Vacuum Society
Date: 12-2016
DOI: 10.1116/1.4972550
Publisher: Elsevier BV
Date: 06-1995
Publisher: Elsevier BV
Date: 12-2015
Publisher: American Vacuum Society
Date: 12-2015
DOI: 10.1116/1.4938024
Publisher: American Vacuum Society
Date: 12-2017
DOI: 10.1116/1.5017990
Publisher: American Chemical Society (ACS)
Date: 23-01-2015
DOI: 10.1021/AM506951C
No related grants have been discovered for Katharina Maniura.