ORCID Profile
0000-0002-5830-8943
Current Organisation
CSIRO
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Publisher: Elsevier BV
Date: 02-2020
DOI: 10.1016/J.JOEN.2019.10.020
Abstract: Mixtures of clodronate with sodium hypochlorite (NaOCl) better maintain free available chlorine (FAC) than etidronate-hypochlorite mixtures. This research aimed to compare organic tissue dissolution and residual FAC between clodronate and etidronate mixtures. Additionally, clodronate-hypochlorite mixtures lose no FAC over several hours. The second aim was to examine how well such mixtures dissolve organic material 6 hours from mixing. Soon after mixing, porcine palatal mucosa s les were added to 32°C solutions containing 0.26 mol/L clodronate and 5% NaOCl (0.26 mol/L-5% NaOCl), 0.26 mol/L etidronate-5% NaOCl, 5% NaOCl, 0.26 mol/L clodronate, 0.26 mol/L etidronate, or phosphate-buffered saline. Weights and FAC, where applicable, were recorded initially and at 15 minutes. FAC was measured by iodometric titration. Secondly, 6 hours after mixing, mucosa was added to 0.26 mol/L clodronate-2.5% NaOCl, 2.5% NaOCl, 0.52 mol/L clodronate-5% NaOCl, 5% NaOCl, or phosphate-buffered saline. S le weights at 0, 5, 10, and 15 minutes were recorded. Analysis of variance was used for statistical analyses (α < .05). Soon after mixing, 0.26 mol/L clodronate-5% NaOCl dissolved mucosa as well as 5% NaOCl and better than 0.26 mol/L etidronate-5% NaOCl compared with which it retained more FAC. At 6 hours after mixing, 0.26 mol/L clodronate-2.5% NaOCl dissolved organic material as well as 2.5% NaOCl. However, 0.52 mol/L clodronate-5% NaOCl dissolved less mucosa than 5% NaOCl. Soon after mixing, clodronate mixtures better dissolve organic material than etidronate mixtures and have higher residual FAC. Six hours from mixing, 0.26 mol/L clodronate-2.5% NaOCl mixtures dissolve organic material similarly to controls.
Publisher: Elsevier BV
Date: 03-2023
Publisher: Springer Science and Business Media LLC
Date: 09-10-2017
DOI: 10.1038/NSMB.3484
Publisher: Elsevier BV
Date: 06-2022
DOI: 10.1016/J.JMB.2021.167408
Abstract: Detecting viral and vector integration events is a key step when investigating interactions between viral and host genomes. This is relevant in several fields, including virology, cancer research and gene therapy. For ex le, investigating integrations of wild-type viruses such as human papillomavirus and hepatitis B virus has proven to be crucial for understanding the role of these integrations in cancer. Furthermore, identifying the extent of vector integration is vital for determining the potential for genotoxicity in gene therapies. To address these questions, we developed isling, the first tool specifically designed for identifying viral integrations in both wild-type and vector from next-generation sequencing data. Isling addresses complexities in integration behaviour including integration of fragmented genomes and integration junctions with ambiguous locations in a host or vector genome, and can also flag possible vector recombinations. We show that isling is up to 1.6-fold faster and up to 170% more accurate than other viral integration tools, and performs well on both simulated and real datasets. Isling is therefore an efficient and application-agnostic tool that will enable a broad range of investigations into viral and vector integration. These include comparisons between integrations of wild-type viruses and gene therapy vectors, as well as assessing the genotoxicity of vectors and understanding the role of viruses in cancer.
Publisher: Elsevier BV
Date: 06-2022
Publisher: Elsevier BV
Date: 06-2020
Publisher: Elsevier BV
Date: 03-2022
Publisher: SPIE
Date: 08-02-2017
DOI: 10.1117/12.2251227
Publisher: Springer Science and Business Media LLC
Date: 19-11-2018
Publisher: Elsevier BV
Date: 02-2019
DOI: 10.1016/J.SBI.2019.03.023
Abstract: GABA
Publisher: Wiley
Date: 09-09-2020
DOI: 10.1111/AEJ.12440
Abstract: Continuous chelation involves the simultaneous use of sodium hypochlorite and a chelating agent. Given the combination of a proteolytic agent and a demineralising chelator, this study aimed to investigate whether mixtures containing the weak chelators etidronate or clodronate and sodium hypochlorite could adversely affect the mechanical strength of teeth compared to the sequence sodium hypochlorite/EDTA/sodium hypochlorite. Matching pairs of bovine teeth were tested on a universal testing machine, and the compressive load at fracture was recorded. One root from each pair was prepared with the sequence, and the matching tooth was prepared with either water, the clodronate mixture or the etidronate mixture. No differences in load at fracture were seen between either mixture and the sequence. However, loads were higher in the teeth irrigated with water compared to the sequence. The results indicated that the continuous chelation mixtures did not alter tooth mechanical properties compared to the standard sequence.
Publisher: Cold Spring Harbor Laboratory
Date: 30-09-2022
DOI: 10.1101/2022.09.28.510021
Abstract: The liver is a prime target for in vivo gene therapies using recombinant adeno-associated viral vectors (rAAV). Multiple clinical trials have been undertaken for this target in the past 15 years, however we are still to see market approval of the first liver-targeted AAV-based gene therapy. Inefficient expression of the therapeutic transgene, vector-induced liver toxicity and capsid, and/or transgene-mediated immune responses reported at high vector doses are the main challenges to date. One of the contributing factors to the insufficient clinical outcomes, despite highly encouraging preclinical data, is the lack of robust, biologically- and clinically-predictive preclinical models. To this end, this study reports findings of a functional evaluation of six AAV vectors in twelve preclinical models of the human liver, with the aim to uncover which model is the most relevant for the selection of AAV capsid variant for safe and efficient transgene delivery to primary human hepatocytes. The results, generated by studies in models ranging from immortalized cells, iPSC-derived and primary hepatocytes, and primary human hepatic organoids to in vivo models, increased our understanding of the strengths and weaknesses of each system. This should allow the development of novel gene therapies targeting the human liver.
Publisher: American Association for the Advancement of Science (AAAS)
Date: 09-09-2020
DOI: 10.1126/SCITRANSLMED.ABA3312
Abstract: AAVs isolated from human liver are tropic for human hepatocytes in vivo, but this phenotype becomes attenuated after passage in culture.
Publisher: Elsevier BV
Date: 02-2015
Publisher: Elsevier BV
Date: 06-2021
Publisher: Springer Science and Business Media LLC
Date: 24-05-2016
DOI: 10.1038/SREP26317
Abstract: Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca 2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.
Location: Australia
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Suzanne Scott.