ORCID Profile
0000-0001-5312-6297
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Publisher: Springer Science and Business Media LLC
Date: 05-03-2016
Publisher: Springer Science and Business Media LLC
Date: 03-2010
DOI: 10.1038/NATURE08850
Publisher: Elsevier BV
Date: 03-2019
DOI: 10.1016/J.FGB.2018.12.008
Abstract: Trichothecene mycotoxin synthesis in the phytopathogen Fusarium graminearum involves primarily endoplasmic reticulum (ER)-localized enzymes of the mevalonate- and trichothecene biosynthetic pathways. Two exceptions are 3-hydroxy-3-methylglutaryl CoA synthase (Hms1) and trichodiene synthase (Tri5), which are known cytosolic enzymes. Using 3D structured illumination microscopy (3D SIM), GFP-tagged Tri5 and Hms1 were tested for preferential localization in the cytosol proximal to the ER. Tri5 protein was significantly enriched in cytosolic regions within 500 nm of the ER, but Hms1 was not. Spatial organization of enzymes in the cytosol has potential relevance for pathway efficiency and metabolic engineering in fungi and other organisms.
Publisher: Public Library of Science (PLoS)
Date: 22-12-2011
Location: United States of America
No related grants have been discovered for Harold Kistler.