ORCID Profile
0000-0002-8378-9021
Current Organisation
National Institutes of Health
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Publisher: Springer Science and Business Media LLC
Date: 06-01-2014
Abstract: Shiga toxin-producing Escherichia coli (STEC) is recognized as an important human diarrheal pathogen. Swine plays an important role as a carrier of this pathogen. In this study we determined the prevalence and characteristics of STEC from healthy swine collected between May 2011 and August 2012 from 3 cities rovinces in China. A total of 1003 s les, including 326 fecal, 351 small intestinal contents and 326 colon contents s les, was analyzed. Two hundred and fifty five s les were stx -positive by PCR and 93 STEC isolates were recovered from 62 stx -positive s les. Twelve O serogroups and 19 O:H serotypes including 6 serotypes (O100:H20/[H20], O143:H38/[H38], O87:H10, O172:H30/[H30], O159:H16, O9:H30/[H30]) rarely found in swine and ruminants were identified. All 93 STEC isolates harbored stx 2 only, all of which were stx 2e subtype including 1 isolate being a new variant of stx 2e . 53.76%, 15.05% and 2.15% STEC isolates carried astA , hlyA and ehxA respectively. Four STEC isolates harbored the high-pathogenicity island. Of the 15 adherence-associated genes tested, 13 ( eae , efa1 , iha , lpfA O113 , lpfA O157/OI-154 , lpfA O157/OI-141 , toxB , saa , F4, F5, F6, F17 or F41) were all absent while 2 ( paa and F18) were present in 7 and 4 STEC isolates respectively. The majority of the isolates were resistant to tetracycline (79.57%), nalidixic acid (78.49%), trimethoprim-sulfamethoxazole (73.12%) and kanamycin (55.91%). The STEC isolates were ided into 63 pulsed-field gel electrophoresis patterns and 21 sequence types (STs). Isolates of the same STs generally showed the same or similar drug resistance patterns. A higher proportion of STEC isolates from Chongqing showed multidrug resistance with one ST (ST3628) resistant to 14 antimicrobials. Our results indicate that swine is a significant reservoir of STEC strains in China. Based on comparison by serotypes and sequence types with human strains and presence of virulence genes, the swine STEC may have a low potential to cause human disease.
Publisher: Public Library of Science (PLoS)
Date: 30-05-2013
Publisher: Public Library of Science (PLoS)
Date: 11-06-2013
Publisher: Microbiology Society
Date: 07-2015
DOI: 10.1099/IJS.0.000228
Abstract: The taxonomic position of a group of seven closely related lactose-negative enterobacterial strains, which were isolated from fresh faecal s les of Marmota himalayana collected from the Qinghai-Tibetan plateau, China, was determined by using a polyphasic approach. Cells were Gram-reaction-negative, non-sporulating, non-motile, short rods (0.5–1 × 1–2.5 μm). By 16S rRNA gene sequences, the representative strain, HT073016 T , showed highest similarity values with Escherichia fergusonii ATCC 35469 T at 99.3 %, Escherichia coli ATCC 11775 T at 99.2 %, Escherichia albertii LMG 20976 T at 98.9 %, Escherichia hermannii CIP 103176 T at 98.4 %, and Escherichia vulneris ATCC 33821 T at 97.7 %. Phylogenetic analysis based on the 16S rRNA gene sequences showed that the seven strains formed a monophyletic group with five other species of the genus Escherichia. Digital DNA–DNA hybridization studies between strain HT073016 T and five other species of the genus Escherichia showed that it shared less than 70 % DNA–DNA relatedness with all known species of the genus Escherichia , supporting the novel species status of the strain. The DNA G+C content of strain HT073016 T was 53.8 mol%. On the basis of phenotypic and phylogenetic characteristics, strain HT073016 T and the six other HT073016 T -like strains were clearly distinct from the type strains of other recognized species of the genus Escherichia and represent a novel species of the genus Escherichia , for which the name Escherichia marmotae sp. nov. is proposed, with HT073016 T ( = CGMCC 1.12862 T = DSM 28771 T ) as the type strain.
Publisher: Elsevier BV
Date: 06-2013
DOI: 10.1016/J.MEEGID.2013.02.018
Abstract: Enterohemorrhagic Escherichia coli O157:H7 is a well-known pathogen as a cause of diarrhea, hemorrhagic colitis (HC), and hemolytic uremic syndrome (HUS). Single nucleotide polymorphisms (SNPs) have been widely used to determine genetic relatedness and epidemiological relationship of O157:H7. Little is known of genetic ersity of Chinese O157:H7 isolates and their relationships with global isolates. The minimum sets of 32 SNPs each from Manning et al. and Clawson et al. were used to type 325 Chinese O157:H7 isolates. The 64 SNPs ided the Chinese O157:H7 isolates into 5 SNP genotypes (SG-1-SG-5). The most common SGs were SG-5 (79.69%) and SG-1 (14.46%). Human isolates concentrated in SG-1 and SG-5, and there is only 1 human isolates in SG-3. The 47 isolates in SG-1 were further ided by an additional SNP sourced from Xuzhou21 genome into 2 subtypes (SG-1.1 and SG-1.2). Strains in SG-1.1 caused the 1999 Xuzhou deadly outbreak. Our Chinese isolates have been found to belong to a limited number of SNP genotypes and are represented by distantly related clades in Manning et al. and lineages in Claswon et al., suggesting parallel spread of these SNP genotypes in China.
Publisher: Informa UK Limited
Date: 2016
DOI: 10.1038/EMI.2016.122
Publisher: Public Library of Science (PLoS)
Date: 03-03-2014
Publisher: American Society for Microbiology
Date: 08-2013
DOI: 10.1128/JCM.00535-13
Abstract: Bacterial pathogens impose a heavy health burden worldwide. In the new era of high-throughput sequencing and online bioinformatics, real-time genome typing of infecting agents, and in particular those with potential severe clinical outcomes, holds promise for guiding clinical care to limit the detrimental effects of infections and to prevent potential local or global outbreaks. Here, we sequenced and compared 85 isolates of Streptococcus suis , a zoonotic human and swine pathogen, wherein we analyzed 32 recognized serotypes and 75 sequence types representing the ersity of the species and the human clinical isolates with high public health significance. We found that 1,077 of the 2,469 genes are shared by all isolates. Excluding 201 common but mobile genes, 876 genes were defined as the minimum core genome (MCG) of the species. Of 190,894 single-nucleotide polymorphisms (SNPs) identified, 58,501 were located in the MCG genes and were referred to as MCG SNPs. A population structure analysis of these MCG SNPs classified the 85 isolates into seven MCG groups, of which MCG group 1 includes all isolates from human infections and outbreaks. Our MCG typing system for S. suis provided a clear separation of groups containing human-associated isolates from those containing animal-associated isolates. It also separated the group containing outbreak isolates, including those causing life-threatening streptococcal toxic shock-like syndrome, from sporadic or less severe meningitis or bacteremia-only isolates. The typing system facilitates the application of genome data to the fields of clinical medicine and epidemiology and to the surveillance of S. suis . The MCG groups may also be used as the taxonomical units of S. suis to define bacterial subpopulations with the potential to cause severe clinical infections and large-scale outbreaks.
Publisher: American Society for Microbiology
Date: 11-2014
DOI: 10.1128/JCM.02282-14
No related grants have been discovered for Qiong Meng.