ORCID Profile
0000-0002-5053-7370
Current Organisations
Paul Scherrer Institute
,
KU Leuven
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: American Physical Society (APS)
Date: 18-05-2020
Publisher: American Physical Society (APS)
Date: 07-04-2021
Publisher: American Physical Society (APS)
Date: 07-04-2021
Publisher: Elsevier BV
Date: 06-2019
Publisher: American Physical Society (APS)
Date: 27-04-2021
Publisher: American Physical Society (APS)
Date: 07-04-2021
Publisher: American Physical Society (APS)
Date: 17-10-2023
Publisher: The Royal Society
Date: 07-10-2019
Abstract: Nucleariid amoebae (Opisthokonta) have been known since the nineteenth century but their ersity and evolutionary history remain poorly understood. To overcome this limitation, we have obtained genomic and transcriptomic data from three Nuclearia , two Pompholyxophrys and one Lithocolla species using traditional culturing and single-cell genome (SCG) and single-cell transcriptome lification methods. The phylogeny of the complete 18S rRNA sequences of Pompholyxophrys and Lithocolla confirmed their suggested evolutionary relatedness to nucleariid amoebae, although with moderate support for internal splits. SCG lification techniques also led to the identification of probable bacterial endosymbionts belonging to Chlamydiales and Rickettsiales in Pompholyxophrys . To improve the phylogenetic framework of nucleariids, we carried out phylogenomic analyses based on two datasets of, respectively, 264 conserved proteins and 74 single-copy protein domains. We obtained full support for the monophyly of the nucleariid amoebae, which comprise two major clades: (i) Parvularia–Fonticula and (ii) Nuclearia with the scaled genera Pompholyxophrys and Lithocolla . Based on these findings, the evolution of some traits of the earliest- erging lineage of Holomycota can be inferred. Our results suggest that the last common ancestor of nucleariids was a freshwater, bacterivorous, non-flagellated filose and mucilaginous amoeba. From the ancestor, two groups evolved to reach smaller ( Parvularia–Fonticula ) and larger ( Nuclearia and related scaled genera) cell sizes, leading to different ecological specialization. The Lithocolla + Pompholyxophrys clade developed exogenous or endogenous cell coverings from a Nuclearia -like ancestor. This article is part of a discussion meeting issue ‘Single cell ecology’.
Publisher: American Physical Society (APS)
Date: 08-06-2018
Location: India
No related grants have been discovered for Piet Van Duppen.