ORCID Profile
0000-0002-9899-7712
Current Organisation
Shandong University
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Publisher: American Chemical Society (ACS)
Date: 27-08-2018
Abstract: Wastewater treatment plants (WWTPs) are considered reservoirs of viruses, but the ersity and dynamic changes of viruses are not well understood. In this study, we recovered 8478 metagenomic viral contigs (mVCs >5 kb) from two WWTPs (Shatin, 2806 Shek Wu Hui, 5672) in Hong Kong. Approximately 60% of the mVCs were poorly covered (<35% of genes in identified mVCs) by the current NCBI and IMG/VR viral databases. The temporal profile of the newly identified mVCs among 98 Shatin AS s les collected monthly (for approximately 9 years) revealed the presence of periodic dynamics at an interval of approximately one year (341 days). The spatial distribution pattern of the virome in the wastewater treatment systems showed that shared viral clusters (viral populations categorized based on shared gene content and network analysis) can be globally found among similar s les of wastewater treatment systems, indicating the presence of core viral communities among geographically isolated wastewater treatment systems. These results not only supplemented the current virome database of engineered systems but also, to some extent, expanded the understanding of long-term cyclical development and spatial distributions of viral communities in wastewater treatment systems.
Publisher: Springer Science and Business Media LLC
Date: 13-02-2018
Publisher: Springer Science and Business Media LLC
Date: 08-10-2019
DOI: 10.1038/S41467-019-12574-Y
Abstract: Several recent studies have shown the presence of genes for the key enzyme associated with archaeal methane/alkane metabolism, methyl-coenzyme M reductase (Mcr), in metagenome-assembled genomes (MAGs) ergent to existing archaeal lineages. Here, we study the mcr -containing archaeal MAGs from several hot springs, which reveal further expansion in the ersity of archaeal organisms performing methane/alkane metabolism. Significantly, an MAG basal to organisms from the phylum Thaumarchaeota that contains mcr genes, but not those for ammonia oxidation or aerobic metabolism, is identified. Together, our phylogenetic analyses and ancestral state reconstructions suggest a mostly vertical evolution of mcrABG genes among methanogens and methanotrophs, along with frequent horizontal gene transfer of mcr genes between alkanotrophs. Analysis of all mcr -containing archaeal MAGs/genomes suggests a hydrothermal origin for these microorganisms based on optimal growth temperature predictions. These results also suggest methane/alkane oxidation or methanogenesis at high temperature likely existed in a common archaeal ancestor.
Publisher: Springer Science and Business Media LLC
Date: 06-10-2021
DOI: 10.1186/S40168-021-01151-5
Abstract: Microbial communities in both natural and applied settings reliably carry out myriads of functions, yet how stable these taxonomically erse assemblages can be and what causes them to transition between states remains poorly understood. We studied monthly activated sludge (AS) s les collected over 9 years from a full-scale wastewater treatment plant to answer how complex AS communities evolve in the long term and how the community functions change when there is a disturbance in operational parameters. Here, we show that a microbial community in activated sludge (AS) system fluctuated around a stable average for 3 years but was then abruptly pushed into an alternative stable state by a simple transient disturbance (bleaching). While the taxonomic composition rapidly turned into a new state following the disturbance, the metabolic profile of the community and system performance remained remarkably stable. A total of 920 metagenome-assembled genomes (MAGs), representing approximately 70% of the community in the studied AS ecosystem, were recovered from the 97 monthly AS metagenomes. Comparative genomic analysis revealed an increased ability to aggregate in the cohorts of MAGs with correlated dynamics that are dominant after the bleaching event. Fine-scale analysis of dynamics also revealed cohorts that dominated during different periods and showed successional dynamics on seasonal and longer time scales due to temperature fluctuation and gradual changes in mean residence time in the reactor, respectively. Our work highlights that communities can assume different stable states under highly similar environmental conditions and that a specific disturbance threshold may lead to a rapid shift in community composition.
Publisher: American Chemical Society (ACS)
Date: 26-06-2018
Abstract: Temporal microbial community studies have broadened our knowledge of the dynamics and correlations among microbes in both natural and artificial engineering systems. Using activated sludge as a model system, we utilized the intensive longitudinal s ling method to identify overlooked ersity and the hidden dynamics of microbes, detect cross-associations among microbes after detrending, and reveal the central microbial dynamics during sludge bulking and foaming. We discovered that the accumulative alpha ersity in activated sludge s led daily over 392 days could be as high as 14 000 OTUs, and that the bacterial community dynamics followed a gradual succession, drifting away from the initial observed day and displaying a significant time-dependent trend. Cross-associations among bacteria were modulated after removing potential spurious correlations based on autocorrelation in microbial time series. Moreover, clusters of bacteria displaying rapid turnover were discovered during the beginning, ongoing, and fading of sludge bulking and foaming, and their physicochemical parameters are resolved. These identified groups of bacteria and their related environmental factors could potentially supply clues to form hypotheses for treating operational problems, such as sludge bulking and foaming.
Publisher: Springer Science and Business Media LLC
Date: 28-11-2017
No related grants have been discovered for Yulin Wang.