ORCID Profile
0000-0002-4872-8928
Current Organisation
University of Nottingham
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Publisher: Elsevier BV
Date: 02-2012
Publisher: Elsevier BV
Date: 2011
DOI: 10.1016/J.BIOS.2010.10.016
Abstract: We report the fabrication and use of electrodes constructed from single walled carbon nanotubes (SWCNTs) chemically assembled on a carbon surface and functionalised with an osmium(II) bipyridine complex (Osbpy). The ability of the electrodes to transduce biologically generated currents from Proteus vulgaris has been established. Our investigations show that there are two contributions to the current: one from electroactive species secreted into solution and another from cell redox sites. The modified electrode can be used to monitor cell metabolism, thereby acting as a whole cell biosensor. The biosensor was used in a 1-h assay to investigate the toxicity of ethanol, sodium azide and the antibiotic icillin and gave quantitative data that were closely correlated with standard cell plate viability assays. The results provide proof of principle that the whole cell biosensor could be used for high throughput screening of antimicrobial activity. One of the modified electrodes was used for approximately 1000 measurements over four months demonstrating the robustness of the system.
Publisher: American Chemical Society (ACS)
Date: 05-10-2011
DOI: 10.1021/AC201769T
Abstract: We describe the reproducible fabrication of robust, vertically aligned multiwalled carbon nanotube (VACNT)/epoxy composite electrodes. The electrodes are characterized by cyclic voltammetry, impedance spectroscopy, and scanning electron and atomic force microscopies. Low background currents are obtained at the electrodes, and common redox probe molecules and NADH show excellent voltammetric behavior. When electrode performance deteriorates due to fouling, the electrode surfaces can be reproducibly renewed by mechanical polishing followed by O(2) plasma treatment. The electrochemical performance of the electrodes is maintained after more than 100 cycles of use and renewal.
Publisher: Elsevier BV
Date: 05-2011
DOI: 10.1016/J.BIOS.2011.02.011
Abstract: Yeast microbial fuel cells have received little attention to date. Yeast should be ideal MFC catalyst because they are robust, easily handled, mostly non-pathogenic organisms with high catabolic rates and in some cases a broad substrate spectrum. Here we show that the non-conventional yeast Arxula adeninvorans transfers electrons to an electrode through the secretion of a reduced molecule that is not detectable when washed cells are first resuspended but which accumulates rapidly in the extracellular environment. It is a single molecule that accumulates to a significant concentration. The occurrence of mediatorless electron transfer was first established in a conventional microbial fuel cell and that phenomenon was further investigated by a number of techniques. Cyclic voltammetry (CV) on a yeast pellet shows a single peak at 450 mV, a scan rate study showed that the peak was due to a solution species. CVs of the supernatant confirmed a solution species. It appears that, given its other attributes, A. adeninivorans is a good candidate for further investigation as a MFC catalyst.
Publisher: American Chemical Society (ACS)
Date: 14-10-2015
Publisher: Wiley
Date: 25-01-2014
Publisher: Elsevier BV
Date: 05-2011
DOI: 10.1016/J.BIOS.2011.01.016
Abstract: Previously we reported an electrochemical method to quantitatively detect vertebrate oestrogens using wild type Saccharomyces cerevisiae cells. That assay required the use of a double mediator system, a five-hour incubation period and had a maximum detection limit of around 11 nM 17β-oestradiol. In the work reported here we have sought to systematically increase the utility and decrease the complexity of the whole cell assay. The steps we took to achieve this goal were in order lysing the cells to remove transport constraints, removing the lipophilic mediator and conducting the assay with the hydrophilic mediator only and finally performing the assay in a complex medium to demonstrate its specificity. Linear sweep voltammetry was used to investigate the interaction of mediators with NADH. The assay is now cell free and functions in a complex substrate. The linear response range upper limit has been raised to 100 nM with a calculated limit of detection of 0.005 nM with a limit of determination of 0.014 nM and the assay period has been reduced to 20 min.
Publisher: American Chemical Society (ACS)
Date: 05-2014
DOI: 10.1021/AM501613C
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Frankie Rawson.