ORCID Profile
0000-0001-7203-7185
Current Organisations
University of Zurich
,
Cape Peninsula University of Technology
,
Universität Zürich
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: Springer Singapore
Date: 2016
Publisher: Elsevier
Date: 2018
Publisher: SAGE Publications
Date: 19-12-2019
Abstract: Determining the tensile strength of nonwoven fabrics is one of the important factors considered for operational performance of the fabrics, especially for fabrics that are exposed to acidic condition. Polyacrylonitrile (PAN), polyphenylene sulfide (PPS) and polyimide (PI) fibres are used to produce fabrics that can withstand harsh chemical condition and still possess the required tensile strength however, over a period of time the tensile strength gradually decreases. The Box–Behnken design method results showed that it can model and describe the effects of process parameters on the tensile strength of spunlaced fabrics in both cross direction and machine direction. The contour plots’ results indicate that varying the fibres polyacrylonitrile (−1), polyphenylene sulfide (0) and polyphenylene sulphide olyimide (1) from −1 to 1 increases the fabric tensile strength in both machine direction and cross direction. For water jet pressure 60 bar (−1), 80 bar (0) and 100 bar (1), increasing the pressure from −1 to 1 increases the fabric tensile strength in cross direction but in the machine direction the fabric strength decreases. Increasing the fabric area weight of 440 g/m 2 (−1), 500 g/m 2 (0) and 560 g/m 2 (1) from −1 to 1 decreases the tensile strength. Exposing fabrics to sulphuric acid (H2SO4) decreases the fabric tensile strength in both machine direction and cross direction due to the degradation of the fibres and the loss decreases gradually with the duration of exposure. For PAN fabrics, the tensile strength decreases by 21% and that of PPS and PPS/PI fabrics decrease by 10% and 6%, respectively.
Publisher: Elsevier BV
Date: 04-2020
Publisher: Proceedings of the National Academy of Sciences
Date: 26-10-1999
Abstract: This study aimed to exploit bacterial artificial chromosomes (BAC) as large antigen-capacity DNA vaccines (BAC-VAC) against complex pathogens, such as herpes simplex virus 1 (HSV-1). The 152-kbp HSV-1 genome recently has been cloned as an F-plasmid-based BAC in Escherichia coli (fHSV), which can efficiently produce infectious virus progeny upon transfection into mammalian cells. A safe modification of fHSV, fHSVΔpac, does not give rise to progeny virus because the signals necessary to package DNA into virions have been excluded. However, in mammalian cells fHSVΔpac DNA can still replicate, express the HSV-1 genes, cause cytotoxic effects, and produce virus-like particles. Because these functions mimic the lytic cycle of the HSV-1 infection, fHSVΔpac was expected to stimulate the immune system as efficiently as a modified live virus vaccine. To test this hypothesis, mice were immunized with fHSVΔpac DNA applied intradermally by gold-particle bombardment, and the immune responses were compared with those induced by infection with disabled infectious single cycle HSV-1. Immunization with either fHSVΔpac or disabled infectious single cycle HSV-1 induced the priming of HSV-1-specific cytotoxic T cells and the production of virus-specific antibodies and conferred protection against intracerebral injection of wild-type HSV-1 at a dose of 200 LD 50 . Protection probably was cell-mediated, as transfer of serum from immunized mice did not protect naive animals. We conclude that BAC-VACs per se , or in combination with genetic elements that support replicative lification of the DNA in the cell nucleus, represent a useful new generation of DNA-based vaccination strategies for many viral and nonviral antigens.
Location: South Africa
Location: South Africa
No related grants have been discovered for Asis Patnaik.