ORCID Profile
0009-0005-3527-1518
Current Organisations
Curtin University
,
Universiti Teknologi Malaysia
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Publisher: Springer Science and Business Media LLC
Date: 29-11-2017
DOI: 10.1038/S41522-017-0040-3
Abstract: Colorectal cancer (CRC) remains the third most common cancer worldwide, with a growing incidence among young adults. Multiple studies have presented associations between the gut microbiome and CRC, suggesting a link with cancer risk. Although CRC microbiome studies continue to profile larger patient cohorts with increasingly economical and rapid DNA sequencing platforms, few common associations with CRC have been identified, in part due to limitations in taxonomic resolution and differences in analysis methodologies. Complementing these taxonomic studies is the newly recognized phenomenon that bacterial organization into biofilm structures in the mucus layer of the gut is a consistent feature of right-sided (proximal), but not left-sided (distal) colorectal cancer. In the present study, we performed 16S rRNA gene licon sequencing and biofilm quantification in a new cohort of patients from Malaysia, followed by a meta-analysis of eleven additional publicly available data sets on stool and tissue-based CRC microbiota using Resphera Insight, a high-resolution analytical tool for species-level characterization. Results from the Malaysian cohort and the expanded meta-analysis confirm that CRC tissues are enriched for invasive biofilms (particularly on right-sided tumors), a symbiont with capacity for tumorigenesis ( Bacteroides fragilis ), and oral pathogens including Fusobacterium nucleatum , Parvimonas micra , and Peptostreptococcus stomatis . Considered in aggregate, species from the Human Oral Microbiome Database are highly enriched in CRC. Although no detected microbial feature was universally present, their substantial overlap and combined prevalence supports a role for the gut microbiota in a significant percentage ( %) of CRC cases.
Publisher: Public Library of Science (PLoS)
Date: 21-12-2018
Publisher: Springer Science and Business Media LLC
Date: 09-01-2019
DOI: 10.1038/S41522-018-0078-X
Abstract: In this Article, in the section entitled ‘Quantitative real-time PCR’ within the Supplementary Methods, the probe for the Bacteroides fragilis 16S real-time PCR reaction was listed incorrectly as 5ʹHEX-AGGGACTGGAAGGCTTTACTGCTTC-3ʹBHQ1. The correct probe for Bacteroides fragilis 16S should be listed as 5ʹHEX-ACACGTATCCAACCTGCCCTTTACTCG-3ʹBHQ1. The mistake was a result of a copy and paste error with a different primer set targeting a B. fragilis toxin gene. All qPCR reactions were performed using the correct probe, and therefore no data were affected.
Publisher: Wiley
Date: 10-09-2022
DOI: 10.1111/FWB.13987
Abstract: Coastal aquifers are vital water sources for humanity. Their quality and the ecosystem services they provide depend on the integrity of their subterranean biota. However, current anthropogenic impacts such as climate change effects and coastal population growth place enormous pressure on the sustainability of these environments. Despite the significance of subterranean biota to ecosystem function and the delivery of ecosystem services, stygofauna—groundwater‐dwelling aquatic animals—have until recently been largely ignored in aquifer monitoring and management. This issue is of importance in both coastal and inland zones. Common threats in inland and coastal areas are water extraction, reduced recharge caused by aridification, and pollution, while, in coastal zones, additional complications arise from sea‐level change and salt water ingress. This review examines stygofaunal ersity, impacts, and future conservation challenges in coastal aquifers. Focussing on Australia, we provide a summary of the available data on stygofaunal communities and distributions identify and describe potential threats to these communities across the erse coastal regions of the continent and propose future research priorities with the goal of facilitating the long‐term preservation of these ecosystems on the Australian continent. While we focus this review on Australia, the threats and management issues discussed are relevant globally. Recent subterranean studies in Australia have been primarily undertaken in inland areas, and while coastal data exist, ecological assessment of coastal subterranean ecosystems is incomplete, compromising the efficacy of conservation plans. This review indicates that the Australian continent hosts five major coastal stygofaunal bio ersity areas characterised by heterogeneous community assemblages, involving a total of 17 taxonomic groups spanning microscopic aquatic invertebrates to vertebrates. The most relevant impacts and threats identified were aquifer size reduction, intrusion of seawater, land clearing, contamination, and mining. Given the projections of increasing coastal development and subsequent loss or degradation of habitat during coming decades, it is important to improve our limited understanding of the ecology of coastal aquifers. Future research should include the refinement of biological assessment tools for coastal systems, functional and ecotoxicological studies, and faunal surveys of urban coastal aquifers. The recommendations and guidelines outlined in this review are applicable globally and provide a further step toward the sustainable use of coastal groundwater resources and the maintenance of healthy groundwater ecosystems.
Location: Malaysia
No related grants have been discovered for Thulasi Kumar.