ORCID Profile
0000-0002-1561-5257
Current Organisation
Johns Hopkins Bloomberg School of Public Health
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Publisher: Springer Science and Business Media LLC
Date: 11-07-2015
Publisher: American Society of Tropical Medicine and Hygiene
Date: 06-2016
Publisher: Springer Science and Business Media LLC
Date: 04-2015
Publisher: Springer Science and Business Media LLC
Date: 30-10-2013
Abstract: The rapid diagnostic test (RDT) has been adopted in contemporary malaria control and management programmes around the world as it represents a fast and apt alternative for malaria diagnosis in a resource-limited setting. This study assessed the performance of a HRP-2 LDH based RDT (Parascreen® Pan/Pf) in a laboratory setting utilizing clinical s les obtained from the field. Whole blood s les were obtained from febrile patients referred for malaria diagnosis by clinicians from two different Upazila Health Complexes (UHCs) located near the Bangladesh-India and Bangladesh-Myanmar border where malaria is endemic. RDT was performed on archived s les and sensitivity and specificity evaluated with expert microscopy (EM) and quantitative PCR (qPCR). A total of 327 clinical s les were made available for the study, of which 153 were Plasmodium falciparum- positive and 54 were Plasmodium vivax- positive. In comparison with EM, for P. falciparum malaria, the RDT had sensitivity: 96.0% (95% CI, 91.2-98.3) and specificity: 98.2% (95% CI, 94.6-99.5) and for P. vivax , sensitivity: 90.7% (95% CI, 78.9-96.5) and specificity: 98.9% (95% CI, 96.5-99.7). Comparison with qPCR showed, for P. falciparum malaria, sensitivity: 95.4% (95% CI, 90.5-98.0) and specificity: 98.8% (95% CI, 95.4-99.7) and for P. vivax malaria, sensitivity: 89.0% (95% CI,77.0-95.4) and specificity: 98.8% (95% CI, 96.5-99.7). Sensitivity varied according to different parasitaemia for falciparum and vivax malaria diagnosis. Parascreen® Pan/Pf Rapid test for malaria showed acceptable sensitivity and specificity in border belt endemic areas of Bangladesh when compared with EM and qPCR.
Publisher: Hindawi Limited
Date: 2014
DOI: 10.1155/2014/493754
Abstract: The parasites of genera Haemoproteus, Plasmodium, and Leucocytozoon are well-known avian haematozoa and can cause declined productivity and high mortality in wild birds. The objective of the study was to record the prevalence of haematozoan parasites in a wide range of wetland birds in Bangladesh. Six species of Haemoproteus , seven species of Plasmodium , one unidentified species of Leucocytozoon , and one unidentified microfilaria of the genus Paronchocerca were found. Data on the morphology, size, hosts, prevalence, and infection intensity of the parasites are provided. The overall prevalence among the birds was 29.5% (95 out of 322 birds). Of those, 13.2% (42 of 319) of birds were infected with Haemoproteus spp., 15.1% with Plasmodium spp. (48 of 319) and 0.6% with Leucocytozoon spp. (2 of 319). Two birds were positive for both Haemoproteus sp. and Plasmodium sp. A single resident bird, Ardeola grayii , was found positive for an unidentified microfilaria. Prevalence of infection varied significantly among different bird families. Wild birds of Bangladesh carry several types of haematozoan parasites. Further investigation with a larger s le size is necessary to estimate more accurately the prevalence of haematozoan parasites among wild birds as well as domestic ducks for better understanding of the disease ecology.
Publisher: Oxford University Press (OUP)
Date: 22-11-2017
DOI: 10.1093/JME/TJX196
Abstract: Malaria is a major health burden in the border-belt areas of Bangladesh. There are recent data from adult mosquito collections that a number of vectors are involved in the transmission cycle. However, little information regarding the larval habitats of Anopheles mosquitoes are available in Bangladesh. To start filling this gap, a cross-sectional larval survey was conducted in Bandarban, Bangladesh from October 2011 to September 2012. Descriptive analysis, Poisson regression, spearman correlations and zero-inflated Poisson regression were used to calculate the degree of association between the abundance of larval Anopheles species and environmental factors. From the 300 larval habitats s led, 5,568 Anopheles larvae were collected and of these, 2,263 (40.6%) were identified to species. Collections represented 16 Anopheles species with Anopheles vagus (26.4%, n = 598) as the dominant species. A total of 16 Anopheles larval habitat types were identified. Larval abundance was significantly different (P < 0.05) among habitats with pond (40%, n = 914) and rice field (34%, n = 779) implicated to be the most utilized. Larval abundance varied significantly (P < 0.05) with habitat characteristics. Most of the larvae were collected from sites with a range of pH from 7.0 to 8.0. Data obtained from this study revealed both natural and human-created larval habitats were favorable for anopheline larval survival and development. Such information elucidates plausible drivers of high anopheline ersity, high vector abundance, changes in relative species abundance from historic data, and sustained transmission of malaria in endemic areas of Bangladesh.
Publisher: Elsevier BV
Date: 06-2014
Publisher: American Society for Microbiology
Date: 20-09-2022
DOI: 10.1128/AAC.00418-22
Abstract: As the malaria parasite becomes resistant to every drug that we develop, the identification and development of novel drug candidates are essential. Many studies have screened compounds designed to target the clinically important blood stages.
Publisher: Cold Spring Harbor Laboratory
Date: 31-03-2022
DOI: 10.1101/2022.03.30.486498
Abstract: As the malaria parasite becomes resistant to every drug that we develop, identification and development of novel drug candidates is essential. Many studies have screened compounds designed to target the clinically important blood stages. However, if we are to shrink the malaria map, new drugs that block transmission of the parasite are needed. Sporozoites are the infective stage of the malaria parasite, transmitted to the mammalian host as mosquitoes probe for blood. Sporozoite motility is critical to their ability to exit the inoculation site and establish infection and drug-like compounds targeting motility are effective in blocking infection in the rodent malaria model. In this study, we established a moderate throughput motility assay for sporozoites of the human malaria parasite Plasmodium falciparum , enabling us to screen the 400 drug-like compounds from the Pathogen box provided by Medicines for Malaria Venture for their activity. Compounds exhibiting inhibitory effects on P. falciparum sporozoite motility were further assessed against transmission-blocking activity and asexual stage growth. Five compounds had a significant inhibitory effect on P. falciparum sporozoite motility at 1 μM concentration and four of these compounds also showed significant inhibition on transmission of P. falciparum gametocytes to the mosquito and of these four, three had previously been shown to have inhibitory activity on asexual blood stage parasites. Our findings provide new antimalarial drug candidates that have multi-stage activity.
Publisher: Springer Science and Business Media LLC
Date: 27-07-2012
Publisher: Springer Science and Business Media LLC
Date: 12-2012
Abstract: Accurate diagnosis of malaria is an essential prerequisite for proper treatment and drug resistance monitoring. Microscopy is considered the gold standard for malaria diagnosis but has limitations. ELISA, PCR, and Real Time PCR are also used to diagnose malaria in reference laboratories, although their application at the field level is currently not feasible. Rapid diagnostic tests (RDTs) however, have been brought into field operation and widely adopted in recent days. This study evaluates OnSite (Pf/Pan) antigen test, a new RDT introduced by CTK Biotech Inc, USA for malaria diagnosis in a reference setting. Blood s les were collected from febrile patients referred for malaria diagnosis by clinicians. Subjects were included in this study from two different Upazila Health Complexes (UHCs) situated in two malaria endemic districts of Bangladesh. Microscopy and nested PCR were considered the gold standard in this study. OnSite (Pf/Pan) RDT was performed on preserved whole blood s les. In total, 372 febrile subjects were included in this study. Of these subjects, 229 (61.6%) tested positive for Plasmodium infection detected by microscopy and nested PCR. OnSite (Pf/Pan) RDT was 94.2% sensitive (95% CI, 89.3-97.3) and 99.5% specific (95% CI, 97.4-00.0) for Plasmodium falciparum diagnosis and 97.3% sensitive (95% CI, 90.5-99.7) and 98.7% specific (95% CI, 96.6-99.6) for Plasmodium vivax diagnosis. Sensitivity varied with differential parasite count for both P. falciparum and P. vivax . The highest sensitivity was observed in febrile patients with parasitaemia that ranged from 501–1,000 parasites/μL regardless of the Plasmodium species. The new OnSite (Pf/Pan) RDT is both sensitive and specific for symptomatic malaria diagnosis in standard laboratory conditions.
Location: No location found
Location: United States of America
No related grants have been discovered for Rubayet Elahi.