ORCID Profile
0000-0002-6385-5239
Current Organisations
Instituto Federal do Amazonas
,
Embrapa Amazônia Ocidental
,
Université Claude Bernard Lyon 1 Faculté des Sciences et Technologies
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Publications
Shining Light on Carbon Nitrides: Leveraging Temperature to Understand Optical Gap Variations
Publisher: American Chemical Society (ACS)
Date: 04-06-2018
Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)
Publisher: Elsevier BV
Date: 2018
DOI: 10.1016/J.ECOENV.2017.09.056
Abstract: Endocrine disrupting compounds (EDCs) have the potential to alter fish reproduction at various levels of organization. The aim of this study was to assess the impact of a natural environment with heavily anthropogenic influence on the physiological processes involved in reproduction in the freshwater fish lambari (Astyanax fasciatus) using different biomarkers. Adult males and females were collected in different seasons from two distinct sites in the same watershed: Ponte Nova Reservoir (PN) considered a pristine or small anthropogenic influence reference point and Billings Reservoir (Bil), subjected to a large anthropogenic impact. Biological indices, such as hepatosomatic index and gonadosomatic index (GSI), gonadal histomorphology, fecundity, and biomarkers such as plasma levels of estradiol (E2) as well as hepatic gene expression of its alfa nuclear receptor (ERα), were analyzed. Hepatic vitellogenin (VTG) gene expression was evaluated in both sexes, as an indicator of xenoestrogen exposure. Females collected at PN presented a typical annual variation reflected in GSI, whereas for those s led at Bil the index did not change through the seasons. The higher concentration of E2 in males collected at Bil during spring/2013, together with the detection of VTG gene expression, suggest the presence of EDCs in the water. These EDCs may have also influenced fecundity of females from Bil, which was higher during winter and spring/2013. Gene expression of ERα and ovarian morphology did not differ between fish from both sites. Water conditions from Bil reservoir impacted by anthropic activity clearly interfered mainly with biomarkers of biological effect such as plasma E2 levels and absolute and relative fecundity, but also altered biomarkers of exposure as VTG gene expression. These facts support the notion that waterborne EDCs are capable of causing estrogenic activity in A. fasciatus.
A new G-quadruplex-specific photosensitizer inducing genome instability in cancer cells by triggering oxidative DNA damage and impeding replication fork progression
Publisher: Oxford University Press (OUP)
Date: 16-05-2023
DOI: 10.1093/NAR/GKAD365
Abstract: Photodynamic therapy (PDT) ideally relies on the administration, selective accumulation and photoactivation of a photosensitizer (PS) into diseased tissues. In this context, we report a new heavy-atom-free fluorescent G-quadruplex (G4) DNA-binding PS, named DBI. We reveal by fluorescence microscopy that DBI preferentially localizes in intraluminal vesicles (ILVs), precursors of exosomes, which are key components of cancer cell proliferation. Moreover, purified exosomal DNA was recognized by a G4-specific antibody, thus highlighting the presence of such G4-forming sequences in the vesicles. Despite the absence of fluorescence signal from DBI in nuclei, light-irradiated DBI-treated cells generated reactive oxygen species (ROS), triggering a 3-fold increase of nuclear G4 foci, slowing fork progression and elevated levels of both DNA base damage, 8-oxoguanine, and double-stranded DNA breaks. Consequently, DBI was found to exert significant phototoxic effects (at nanomolar scale) toward cancer cell lines and tumor organoids. Furthermore, in vivo testing reveals that photoactivation of DBI induces not only G4 formation and DNA damage but also apoptosis in zebrafish, specifically in the area where DBI had accumulated. Collectively, this approach shows significant promise for image-guided PDT.
Correction: Light-induced in situ chemical activation of a fluorescent probe for monitoring intracellular G-quadruplex structures
Publisher: Royal Society of Chemistry (RSC)
Date: 2023
DOI: 10.1039/D2NR90225G
Abstract: Correction for ‘Light-induced in situ chemical activation of a fluorescent probe for monitoring intracellular G-quadruplex structures’ by Marco Deiana et al. , Nanoscale , 2021, 13 , 13795–13808, 0.1039/D1NR02855C.
Light-induced in situ chemical activation of a fluorescent probe for monitoring intracellular G-quadruplex structures
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1NR02855C
Abstract: A photochemically generated fluorophore is used to monitor intracellular G-quadruplex structures.
Exploring the Concept of Dimerization-Induced Intersystem Crossing: At the Origins of Spin–Orbit Coupling Selection Rules
Publisher: American Chemical Society (ACS)
Date: 22-07-2021
Abstract: Singlet-triplet interconversions (intersystem crossing, ISC) in organic molecules are at the basis of many important processes in cutting-edge photonic applications (organic light-emitting devices, photodynamic therapy, etc.). Selection rules for these transitions are mainly governed by the spin-orbit coupling (SOC) phenomenon. Although the SOC relies on complex relativistic phenomena, theoreticians have, with time, developed increasingly sophisticated and efficient approaches to gain access to a satisfactory evaluation of its magnitude. However, recent works have highlighted the remarkable and somehow unexpected efficiency of dimers of small conjugated molecules in terms of ISC quantum yields, whose origin has not been completely investigated. In this work, we bring a coupled experimental and theoretical analysis of the origin of the unusually large ISC efficiency on a series of such dimers that differ by their nature (covalent or supramolecular). We show that considering the dynamical nature of the SOC, and especially its dependence on angular orientations between the dimer subunits sometimes overlooked in the literature, it is necessary to rationalize some counterintuitive experimental observations. This combined experimental and theoretical work paves the way for new molecular engineering rules for SOC control.
Related Organisations
Universidade Estadual Paulista Júlio De Mesquita Filho
Location: Brazil
Université Claude Bernard Lyon 1 Faculté Des Sciences Et Technologies
Location: France
Related Funding Activities
No related grants have been discovered for Gabriela Brambila-Souza.