ORCID Profile
0000-0001-7333-5280
Current Organisation
University of Manchester
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Publisher: Elsevier BV
Date: 06-2010
Publisher: Elsevier BV
Date: 12-2022
Publisher: American Chemical Society (ACS)
Date: 13-11-2019
Abstract: Ultrafine particulate aerosols less than 100 nm diffuse randomly in the air and are hazardous to the environment and human health. However, no technical standards or commercial products are available for filtering particle sizes under 100 nm yet. Here, we report the development of a porous poly(l-lactic acid) (PLLA) nanofibrous membrane filter with an ultra-high specific surface area via electrospinning and a post-treatment process. After PLLA fibres were electrospun and collected, they were treated by acetone to generate a blossoming porous structure throughout each in idual fibre. Characterizations of morphology, crystallinity, and mechanical and thermal properties demonstrated that the porous structure can be attributed to the nonsolvent-induced spinodal phase separation during electrospinning and solvent-induced recrystallization during post treatment. The blossoming porous structure with high specific surface area contributed to excellent filtration efficiency (99.99%) for sodium chloride (NaCl) ultrafine aerosol particles (30-100 nm) with a low pressure drop (110-230 Pa). Notably, under 7.8 cm/s air flow rate, the membrane s les performed better for filtering smaller-sized aerosol particles than the larger ones when evaluated by the quality factor (0.07). Finally, this finding demonstrates that the electrospun membrane with a hierarchical pore structure and high specific surface area hold great potential in applications as air-filtering materials.
Publisher: Springer Science and Business Media LLC
Date: 11-05-2013
Publisher: Springer Science and Business Media LLC
Date: 09-08-2016
DOI: 10.1038/SREP31149
Abstract: Antimicrobial peptides, produced by innate immune system of hosts in response to invading pathogens, are capable of fighting against a spectrum of bacteria, viruses, fungi, parasites and cancer cells. Here, a recombinant silkworm AMP Bmattacin2 from heterologous expression is studied, indicating a broad spectrum of antibacterial activity and showing selective killing ability towards skin and colon cancer cells over their normal cell counterparts. For the purpose of biomedical application, the electrospinning fabrication technique is employed to load Bmattacin2 into PLLA nanofibrous membrane. In addition to a good compatibility with the normal cells, Bmattacin2 loaded nanofibrous membranes demonstrate instant antibacterial effects and sustained anticancer effects. The cancer cell and bacteria targeting dynamics of recombinant Bmattacin2 are investigated. With these characteristics, PLLA/Bmattacin2 composite membranes have a great potential for developing novel biomedical applications such as cancer therapies and wound healing treatments.
Publisher: American Chemical Society (ACS)
Date: 04-03-2013
DOI: 10.1021/IE301907F
Publisher: Wiley
Date: 21-03-2012
DOI: 10.1002/JBM.B.32645
Abstract: Taking the inspiration from the biomineral, the wool keratin was selected to modulate the assembly of nanosized hydroxyapatite (HA) crystals via a coprecipitation method. A series of keratin/HA nanocomposite with different ratios were synthesized by adjusting the concentrations of keratin solutions and calcium phosphate and their final components were detected by thermogravimetric analysis (TGA). The transmission electron microscopy (TEM) and X-ray diffraction (XRD) confirmed that keratin in the composite decreased the crystallinity of HA. Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) were used to examine the chemical and surface structure of the composites. In vitro biocompatibility results revealed that cells showed better viability on keratin/HA composites which have a ratio of organics and inorganics similar to that of natural bones.
Publisher: Wiley
Date: 30-05-2013
DOI: 10.1002/JBM.A.34759
Abstract: Biodegradable stents can alleviate intestinal obstruction and stenosis in patients. The objective of this study was to develop a biodegradable polydioxanone (PDO) stent using weft-knitting technology and then investigate its biodegradation behaviors in vitro. PDO monofilament with linear density of 100 ± 10 tex was knitted into a tubular stent using a tubular weft-knitting machine. The physical and mechanical properties were evaluated according to the British standard BS EN 13895:2003 and ISO 7198:1998. The biodegradation behaviors of PDO weft-knitted stent in a phosphate buffer solution (pH = 6.8 ± 0.2, 37 ± 0.5 °C) were then investigated. The results showed that the stent maintained more than 60% of its original radial force above 12 weeks. During the 16 weeks of degradation, weight, crystallization, and pH change indicated the degradation medium was diffused into the chain segments of low molecular weight due to the rupture of ester bonds in the monofilament. Fourier transform infrared spectroscopy results demonstrated that the chemical structure of PDO polymer is stable during the in vitro degradation. In conclusion, this biodegradable stent can find valuable applications in treatment of intestinal obstruction and stenosis clinically.
Publisher: Elsevier BV
Date: 07-2013
DOI: 10.1016/J.FCT.2013.03.047
Abstract: The cytotoxicity of wool polypeptide has been evaluated by both cell and animal models. Wool was dissolved in sodium hydroxide solution, the pH value of the solution was adjusted to 5.55 and the precipitate was harvested as wool polypeptide. The spray-dried polypeptide was collected as powders and characterized by SEM, FTIR and TG-DSC. The cell culturing results showed that wool polypeptide had no obvious negative effect on cell viability in vitro. Both acute oral toxicity and subacute 30-day oral toxicology studies showed that wool polypeptide had no influence on body weight, feed consumption, blood chemistry, and hematology at any dose levels. There were no treatment related findings on gross or detailed necroscopy, organ weights, organ/body weight ratios and histology. Our study indicated the absence of toxicity in wool polypeptide and supported its safe use as a food ingredient or drug carrier.
Publisher: Springer Science and Business Media LLC
Date: 03-2012
Publisher: Elsevier BV
Date: 11-2013
Publisher: Elsevier BV
Date: 05-2016
DOI: 10.1016/J.JTHERBIO.2016.03.008
Abstract: Human skin temperature can vary widely depending on anatomical location and ambient temperature. It is also known that local changes in skin and subcutaneous temperature can affect fat metabolism. This study aimed to explore the potential effects of surrounding thermal environment on fat by investigating cell viability, lipid oxidation, and lipid accumulation in 3T3-L1 adipocytes and palmitate-treated adipocytes after 4h incubation. No significant differences of viability in 3T3-L1 adipocytes were detected under different temperature conditions. Despite no significant increase being observed under warm temperature (39°C) conditions, a similarly significant suppression of intracellular reactive oxygen species (ROS) and lipid peroxidation were found in 3T3-L1 adipocytes and palmitate-treated adipocytes under 4h exposure to cooler temperatures of 31-33°C (P<0.01). ROS, chemically reactive molecules containing oxygen, are currently understood to be a major contributor to oxidantive stress in obesity. Additionally, cooler temperatures (31-33°C) could improve the size of lipid droplets in 3T3-L1 adipocytes (P<0.01), but no significant effect was generated by temperature change on lipid droplets in palmitate-treated adipocytes. In the palmitate-induced adiposity model, although excessive ROS and lipid peroxidation has been attenuated by temperature decrease (P 0.05) and remedy the palmitate damage induced cell death (P<0.01). These findings provide preliminary support for potential interventions based on temperature manipulation for cell metabolism of adipocytes.
Publisher: Elsevier BV
Date: 10-2009
Publisher: Elsevier BV
Date: 08-2015
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4RA11080C
Abstract: Thermoresponsive PNIPAM polymer brushes are grafted onto the surface of cotton fabrics to construct a smart hierarchical system.
Publisher: Elsevier BV
Date: 12-2021
Publisher: SAGE Publications
Date: 24-10-2014
Abstract: The present study introduced a novel biocompatible electrospun membrane composed of poly ( l-lactic acid) (PLLA) and water-insoluble keratin precipitates from wool hydrolyzed solution. Ultrafine nanofibrous membranes were successfully prepared by electrospinning the blend of PLLA and keratin/alcohol suspension. Uniform structure of the composite nanofibers was observed by scanning electron microscopy. Transmission electron microscopy pictures indicated keratin was evenly distributed within the composite fibers. The result of Fourier transform infrared (FT-IR) spectra revealed the composition of the keratin/PLLA membrane. X-ray differential spectroscopy suggested that higher crystallinity can be observed from keratin/PLLA composite nanofibers than pristine keratin precipitates. The thermogravimetric analyzer spectra indicated that keratin/PLLA composite fibers possessed more constant thermostability than pristine keratin precipitates. The in vitro degradation of keratin/PLLA composite nanofibers was evaluated by FT-IR spectra and ultraviolet spectra. The experimental results showed that the degradation rate was fast on the first day and slow in the following period. The biocompatibility of the keratin/PLLA composite membrane was examined by MTT assay and the cell proliferation profile was observed by fluorescent micrographs. The results indicated that with the promoting effect on cell adherence and proliferation, this composite material was suitable as a biomedical fabrics candidate for use in drug delivery vehicle and tissue engineering scaffolds.
Publisher: American Chemical Society (ACS)
Date: 09-02-2015
DOI: 10.1021/AM508498U
Abstract: Because of the intensified competition of land resources for growing food and natural textile fibers, there is an urgent need to reuse and recycle the consumed/wasted natural fibers as regenerated green materials. Although polypeptide was extracted from wool by alkaline hydrolysis, the size of the polypeptide fragments could be reduced to nanoscale. The wool polypeptide particles were fragile and could be crushed down to nano size again and dispersed evenly among polymer matrix under melt extrusion condition. The nano polypeptide particles could reinforce antiultraviolet capability, moisture regain, and mechanical properties of the polymer-polypeptide composite fibers.
Publisher: Hindawi Limited
Date: 2009
DOI: 10.1155/2009/168041
Abstract: Nanosilver has been studied as a valuable material for it strong antibacterial effects. In this study, we investigated the antibacterial properties of nano silver Poly-L-Lactic acid (Ag/PLLA) composite fibrous membranes. Ag/PLLA fibrous membranes were prepared with silver nanoparticles having weight ratio of silver nanoparticles to PLLA at 5% (w/w). In vitro antibacterial tests were performed using Escherichia coli (E. coli) and Staphylococcus aureus (Staph.) to determine the antibacterial capability of the Ag/PLLA fibrous membranes. As the results suggested, Ag/PLLA fibrous membranes showed strong antibacterial properties. Thus, Ag/PLLA fibrous membrane can be used as an antibacterial scaffold for tissue engineering.
Publisher: Elsevier BV
Date: 10-2016
DOI: 10.1016/J.BIOMATERIALS.2016.06.049
Abstract: The development of more effective cancer therapeutic strategies are still critically required. The maximization of the therapeutic effect in combination with avoiding the severe side effects on normal tissues when using chemotherapy drugs is still an urgent problem that requires improvements urgently. Here we provide implantable and controllable drug-release that utilises silk fibroin (SF) as a nanofibrous drug delivery system (DDS) for cancer treatment. A nanofibrous structure with controllable fibre diameter ( 5 μg/mL. The mechanism could be explained by the cell cycle being held in the S phase. The toxic effect on normal cells (NCM460) was minimized by using a treatment concentration range (5-20 μg/mL). Implantation of this DDS into the tumour site inhibited the growth of solid tumour this offers an alternative approach for novel cancer therapy.
Publisher: American Chemical Society (ACS)
Date: 10-12-2019
Abstract: We propose a design strategy to fabricate a flexible bend sensor (BS) with ultrasensitivity toward airflow using all-poly(vinylidene fluoride) (PVDF) nanofiber web-based sensing elements and electrodes to monitor human respiration. The unique electrospinning (rotational speed of collector of 2000 rpm and tip-to-collector distance of 4 cm) with silver nanoparticle interfacing was introduced to prepare a Ag-doped oriented PVDF nanofiber web with high β-phase content as a sensing element (AgOriPVDF, β-phase crystallinity ∼44.5%). After that, a portion of the prepared AgOriPVDF was processed into a flexible and electrically conductive electrode through an electroless silver plating technique (SP-AgOriPVDF). Interestingly, the encapsulated AgOriPVDF BS with the SP-AgOriPVDF electrode exhibited superior piezoelectric bending response (open-circuit peak-to-peak output voltage,
Publisher: Elsevier BV
Date: 10-2022
DOI: 10.1016/J.IJBIOMAC.2022.08.174
Abstract: The intramedullary splint insertion fixation system is the mainstream clinical solution to severe rib fractures. However, the titanium alloy scaffolds have limitations in biocompatibility, flexibility and complexity of surgeries. Here we present a revisable wheel-spun alginate (Alg)/graphene oxide (GO)-based fibrous rod as a potential alternative for a rib internal fixation system. The reversible fusion and fission capability was obtained by optimized Alg/GO blended spinning and GO coating post-treatment. The mechanical performance of the demonstrated rod s les matches the properties of the human rib. A self-designed cubic matrix was used to conduct in situ cell culture. In vitro evaluation not only confirms the cell viability and migration on the fibers' surface, but also investigated the degradation and fission performance of fibrous rods. With a simple, minimally invasive implantation and controlled swelling, Alg/GO fibrous rods are able to tightly fix the rib fracture wound while maintaining sufficient flexibility.
Publisher: Elsevier BV
Date: 03-2016
Publisher: Elsevier BV
Date: 10-2009
Publisher: Elsevier BV
Date: 12-2013
DOI: 10.1016/J.BIOMATERIALS.2013.08.055
Abstract: In-stents restenosis caused by tumour ingrowth is a major problem for patients undergoing stent displacement because the conventional stents often lack a sustained anti-tumour capability. The aim of this paper was to develop a weft-knitted polydioxanone stent which can slow release 5-fluorouracil (5-FU). In order to determine the most suitable drug concentration, the 5-FU safe concentration in vivo and appropriate loading percentage in the membranes were investigated, and then 5-FU-loaded poly-l-lactide membranes at concentration of 3.2%, 6.4% and 12.8% were coated onto the stent using electro-spinning method, respectively. The morphology, chemical structure and in vitro drug release property of the coating membranes were subsequently examined. Their anti-tumour activity and mechanism were assessed in vitro and in vivo using a human colorectal cancer cell line HCT-116 and tumour-bearing BALB/c nude mice. The half maximal inhibitory concentration (IC50) and the median lethal dose (LD50) demonstrated that the 6.4% and 12.8% membranes had better anti-tumour effects than pure 5-FU due to the sustainable drug releasing property of the coated membranes on the stent. The membranes possessing appropriate drug loading doses, such as 6.4% or 12.8% also provided better anti-in-stents restenosis effects than other groups tested. Therefore, it is concluded that the drug-loaded stents have great potential for the use in the treatment of intestinal cancers in the future.
Publisher: Elsevier BV
Date: 09-2013
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C2TB00460G
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Jiashen Li.