ORCID Profile
0000-0003-0434-2457
Current Organisation
Muhammad Nawaz Shareef University of Agriculture
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Publisher: Scientific Societies
Date: 07-2021
Publisher: Scientific Societies
Date: 06-2017
Publisher: Scientific Societies
Date: 05-2020
Publisher: Scientific Societies
Date: 06-2019
Publisher: Scientific Societies
Date: 05-2020
DOI: 10.1094/PDIS-08-19-1743-RE
Abstract: Potato blackleg is caused by a erse species of pectinolytic bacteria. In Pakistan, approximately 90% of the pathogens involved belong to Pectobacterium atrosepticum. Survey (2014 to 2017), s ling, and isolation from different potato growing areas of Punjab, Pakistan depicted an overall disease incidence of approximately 15%. Thirty-six pectinolytic strains confirmed through biochemical and pathogenicity testing were characterized via gapA gene to identify them at the species level. To further validate the identification, one strain from each species SS26 (P. atrosepticum), SS28 (Pectobacterium polaris), SS70 (Dickeya dianthicola), SS90 (Pectobacterium parmentieri), SS95 (Pectobacterium punjabense), and SS96 (Pectobacterium versatile) were selected for draft genome sequencing and multilocus sequence analysis of 13 housekeeping genes (fusA, rpoD, acnA, purA, gyrB, recA, mdh, mtlD, groEL, secY, glyA, gapA, and rplB). Phylogenetic analysis revealed considerable genetic ersity in the genus Pectobacterium. In silico DNA–DNA hybridization and average nucleotide identity values of the strains selected for genome sequencing were determined with other reference Pectobacterium and Dickeya strains. Moreover, all six representative strains were also phenotypically characterized on the basis of metabolism of different carbon sources. Overall, on the basis of genotypic and phenotypic characteristics, these 36 isolates were grouped into six species: P. atrosepticum, P. versatile, P. parmentieri, P. polaris, P. punjabense, and D. dianthicola.
Publisher: MDPI AG
Date: 13-04-2023
Abstract: Citrus canker (CC), caused by one of the most destructive subfamilies of the bacterial phytopathogen Xanthomonas citri subsp. Citri (Xcc), poses a serious threat to the significantly important citrus fruit crop grown worldwide. This has been the subject of ongoing epidemiological and disease management research. Currently, five different forms have been identified of CC, in which Canker A (Xanthomonas citri subsp. citri) being the most harmful and infecting the majority of citrus cultivars. Severe infection symptoms include leaf loss, premature fruit drop, dieback, severe fruit blemishing or discoloration, and a decrease in fruit quality. The infection spreads rapidly through wind, rain splash, and warm and humid climates. The study of the chromosomal and plasmid DNA of bacterium has revealed the evolutionary pattern among the pathovars, and research on the Xcc genome has advanced our understanding of how the bacteria specifically recognize and infect plants, spread within the host, and propagates itself. Quarantine or exclusion programs, which prohibit the introduction of infected citrus plant material into existing stock, are still in use. Other measures include eliminating sources of inoculum, using resistant hosts, applying copper spray for protection, and implementing windbreak systems. The main focus of this study is to highlight the most recent developments in the fields of Xcc pathogenesis, epidemiology, symptoms, detection and identification, host range, spread, susceptibility, and management. Additionally, it presents an analysis of the economic impact of this disease on the citrus industry and suggests strategies to reduce its spread, including the need for international collaboration and research to reduce the impact of this disease on the global citrus industry.
Publisher: Scientific Societies
Date: 07-2021
Publisher: Scientific Societies
Date: 10-2018
Publisher: Pakistan Phytopathological Society
Date: 30-06-2018
DOI: 10.33866/PHYTOPATHOL.030.01.0445
Abstract: Brassica c estris is the leading oilseed crop of Pakistan. It fulfills 20% oil requirement of Pakistan. This crop is affected by various biotic and abiotic factors. Among all other yield limiting biotic constraints, Alternaria blight, caused by Alternaria brassicae playes imperative role. In the current research fifteen advanced lines/genotypes (Punjab Canola, Faisal Canola, Shirale, Dunkeld, Rainbow, AC-Excel, Legend, Oscar, Cyclone, Con II, Con III, Bulbul 98, DGL, B.S.A, Toria selection) were sown at Department of Plant Pathology with three replications under Completely Randomized Block Design (RCBD) by maintaining the plant to plant (P×P) distance of 45 cm and bed to bed (B×B) distance of 75 cm. Two genotypes i.e. Punjab canola (48.83%), Faisal canola (50.06%) exhibited moderately resistant response. Similarly, eight commercially available fungicides namely Rally 40% WP (Myclobutanil 40%), Chlorotoxin (Azoxystrobin 5.4%+Chlorothalonil 45%), Alliette 80% WP (Fosetyli-Aluminium 80%), Success 70% WP (Metalaxyl 8% + Chlorothalonil 64%), Swing 72% (Metalaxyl 8% +Mancozeb 64%), Cytrol 75% (Thiophanate-methyl 35% + Chlorothalonil 40%), Antracol (Propineb 70%) and Score 250 EC (Difenconazole 250EC) were used with two concentrations viz. 0.25% and 0.50% of each fungicide under filed conditions with three replication and Randomized Complete Block Design. It was observed that both concentrations (0.25% and 0.50%) of swing played statistically significant role to inhibit the disease incidence. Therefore, it is concluded that Punjab Canola and Faisal Canola are statistically reliable advanced lines as compared to all other thirteen advanced lines/ genotypes. Likewise, the concentrations 0.25% and 0.50% of swing have statistically more potential to retard the disease incidence as compared to all other commercially available fungicides.
Publisher: Scientific Societies
Date: 06-2017
Publisher: Scientific Societies
Date: 08-2021
Publisher: Public Library of Science (PLoS)
Date: 25-08-2021
DOI: 10.1371/JOURNAL.PONE.0256450
Abstract: Food production and waste recycling are the two major issues faced globally with rapidly increasing population. Recycling organic wastes to crop amendments could be a possible solution to these issues. Earthworms transfer organic waste to compost, which is used to grow crops and increase crop productivity. This study assessed the impact of vermicompost produced from the residues of six desert plant species, i.e., ( Ziziphus mauritiana , Aerva javanica , Calligonum comosum , Sacchrum benghalens , Calligonum polygonoides and Prosopis cineraria ) combined with farmyard manure (5 t ha -1 ) on growth, yield and photosynthetic activity of maize crop. Earthworm species Eisenia fetida (Savigny, 1826) was used to prepare vermicomposting of all tested plant species. The desert species were collected from natural habitats, chopped, dried, mixed with FYM and then earthworms were released to prepare the vermicompost. The earthworms were excluded twenty days after release and resultant was considered as compost and used in the experiment. Results revealed that application of P . cineraria vermicompost resulted in the highest plant height (75.33 cm), stem diameter (22.66 mm), cob length (17.66 cm), number of grains/cob (374.67), 1000-grain weight (260.41 g) and grains yield (3.20 t/ha). Application of P . cineraria vermicompost resulted in the highest uptake of macronutrients, i.e., N (91.01%), P (22.07%), K (80.41%), micronutrients, i.e., Fe (19.07 ppm), Zn (40.05 ppm), and phenolic contents (150). Application of P . cineraria vermicompost also resulted in the highest quantum photosynthetic yield (0.42 mole C/mole of photon), chlorophyll florescence (355.18 moles of photon m -2 s -1 ) and electron transport rate (310.18 micro mole m -2 s -1 ). It is concluded that vermicomposting has the potential to improve growth and yield of maize crop. Particularly, application of vermicompost obtained from P . cineraria can be used to improve the growth and yield of maize crop. Nonetheless, field trials are necessary for a wide scale recommendation.
Publisher: BMJ
Date: 12-2022
DOI: 10.1136/BMJOPEN-2022-062059
Abstract: Epidemiological evidence suggests that both poor cardiovascular fitness and low muscle mass or strength markedly increase the rate of cognitive decline and incident dementia in older adults. Results from exercise trials for the improvement of cognition in older adults with mild cognitive impairment (MCI) have reported mixed results. This is possibly due to insufficient exercise intensities. The aim of the Balance, Resistance, And INterval (BRAIN) Training Trial is to determine the effects of two forms of exercise, high-intensity aerobic interval training (HIIT) and high-intensity power training (POWER) each compared with a sham exercise control group on cognition in older adults with MCI. One hundred and sixty community-dwelling older (≥ 60 years) people with MCI have been randomised into the trial. Interventions are delivered supervised 2–3 days per week for 12 months. The primary outcome measured at baseline, 6 and 12 months is performance on a cognitive composite score measuring the executive domain calculated from a combination of computerised (NeuroTrax) and paper-and-pencil tests. Analyses will be performed via repeated measures linear mixed models and generalised linear mixed models of baseline, 6-month and 12-month time points, adjusted for baseline values and covariates selected a priori. Mixed models will be constructed to determine the interaction of GROUP × TIME. Ethical approval was obtained from the University of Sydney (HREC Ref.2017/368), University of Queensland (HREC Ref. 2017/HE000853), University of British Columbia (H16-03309), and Vancouver Coastal Health Research Institute (V16-03309) Human Research Ethics. Dissemination will be via publications, conference presentations, newsletter articles, social media, talks to clinicians and consumers and meetings with health departments/managers. It is expected that communication of results will allow for the development of more effective evidence-based exercise prescription guidelines in this population while investigating the benefits of HIIT and POWER on subclinical markers of disease. ACTRN12617001440314 Australian New Zealand Clinical Trials Registry.
Publisher: Springer Science and Business Media LLC
Date: 05-08-2020
Publisher: Scientific Societies
Date: 02-2018
Publisher: Springer Science and Business Media LLC
Date: 28-08-2018
Publisher: Elsevier BV
Date: 11-2022
Publisher: ALOKI Ltd
Date: 2019
Publisher: Scientific Societies
Date: 08-2017
Publisher: American Society for Microbiology
Date: 06-08-2020
DOI: 10.1128/MRA.00420-20
Abstract: Pectobacterium punjabense is a newly described species causing blackleg disease in potato plants. Therefore, by the combination of long (Oxford Nanopore Technologies, MinION) and short (Illumina MiSeq) reads, we sequenced the complete genome of P. punjabense SS95 T , which contains a circular chromosome of 4.793 Mb with a GC content of 50.7%.
Publisher: Scientific Societies
Date: 08-2021
Publisher: Scientific Societies
Date: 08-2017
Publisher: MDPI AG
Date: 31-08-2023
Abstract: Water management techniques are improving at the farm level, but they are not enough to deal with the limited availability of water and increased crop yields. Soil microbes play a vital role in nitrogen fixation, improving soil fertility and enhancing plant growth hormones under drought conditions. Therefore, this study was conducted to investigate the impact of water management combined with Azospirillum brasilense and Rhizobium pisi on wheat crop productivity and soil properties in dry regions. Three water management techniques were compared, normal irrigation as a control (C), deficit irrigation (DI), and partial root drying irrigation (PRD), together with the interaction of plant-growth-promoting rhizobacteria (PGPR). Experiments were conducted with six treatments in total: T1 = C + No PGPR, T2 = C + PGPR, T3 = DI + No PGPR, T4 = DI + PGPR, T5 = PRD + No PGPR, and T6 = PRD + PGPR. The highest grain yield was achieved in the control irrigation treatment using seeds inoculated with rhizobacteria, followed by control treatment without any inoculation, and the lowest was recorded with deficit irrigation without rhizobacteria inoculated in the seeds. However, PRD irrigation resulted in significantly higher plant growth and grain yield than the DI treatment. PGPR inoculation combined with PRD resulted in a 22% and 20% higher number of grains per spike, a 19% and 21% higher grain yield, and a 25% and 22% higher crop growth rate compared to rhizobacteria inoculation combined with the DI system in 2021-22 and 2022-23, respectively. This increase was due to the higher production of growth hormones and higher leaf area index under water-limited conditions. A greater leaf area index leads to a higher chlorophyll content and higher food production for plant growth.
Publisher: Springer Science and Business Media LLC
Date: 04-01-2019
Publisher: Scientific Societies
Date: 10-2019
Location: Canada
Location: Pakistan
Location: Pakistan
Start Date: 2019
End Date: 2020
Funder: Higher Education Commission, Pakistan
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