ORCID Profile
0000-0002-6376-2630
Current Organisation
University of Melbourne
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Publisher: American Chemical Society (ACS)
Date: 15-07-2021
Publisher: Elsevier BV
Date: 06-2018
DOI: 10.1016/J.WATRES.2018.02.041
Abstract: Production of taste and odour (T/O) compounds, principally geosmin, by complex cyanobacterial blooms is a major water quality issue globally. Control of these cyanobacteria imposes a significant cost on water producing and dependent industries, and requires routine monitoring and management. Classic monitoring methods, including microscopy and direct chemical analysis, lack sensitivity, are laborious, expensive or cannot reliably identify the source of geosmin production. Polymerase Chain Reaction (PCR) based tools targeting the geosmin synthase gene (geoA) provide a novel tool for routine monitoring. However, geoA is variable at the nucleotide level and potential geosmin producers represent a broad taxonomic distribution, such that multiple PCR primers with distinct lification protocols are needed to target all potential sources of this important T/O compound. Development of novel primers is hindered by a lack of sequence data and limited field and laboratory data on geosmin producers prevents prioritizing taxa for PCR testing. Here we performed a genetic screen of 253 bloom s les from Victoria, Australia using each existing PCR protocol targeting geoA. We detected Dolichospermum ucrainicum as the major geosmin producer (87% of sequenced s les) along with 3 unknown geoA sequence types. Using these data, we designed a novel, short licon, PCR protocol utilising a single standardised primer pair, capable of lifying all geoA positive s les in our study, as well as a Nostoc punctiforme positive control. This single protocol geoA PCR can further be tested on other geosmin producers and will simplify routine monitoring of T/O producing cyanobacteria.
Publisher: Springer Science and Business Media LLC
Date: 26-07-2019
DOI: 10.1038/S41598-019-46945-8
Abstract: Routine monitoring of toxic cyanobacteria depends on up-to-date epidemiological information about their distribution. In Australia, anatoxin producing cyanobacteria are not regularly tested for and thought to be rare if not absent from the continent. Our study investigated the presence of anatoxin-a (ATX-a) producing cyanobacteria in surface water s les (n = 226 from 67 s ling locations) collected from 2010 to 2017 across the state of Victoria, Australia. We (1) detected the presence and distribution of ana C (anatoxin-a synthetase C) gene sequences previously associated with various cyanobacteria, including Cuspidothrix issatschenkoi , Aphanizomenon sp., D. circinale, Anabaena sp., and Oscillatoria sp., from 31 s ling locations, and (2) determined the concentration of ATX-a in s les tested using ELISA, in two instances detected at µg · L −1 . These data present the first confirmation of ATX-a producers in Australia. Our study indicates that ATX-a should be included in regular testing of cyanobacterial blooms in Australia and highlights the importance of regular investigation of the distributions of toxic cyanobacteria worldwide, particularly amid the known expanding distribution of many cyanobacterial taxa in a period of increased eutrophication and rising surface water temperatures.
Publisher: Elsevier BV
Date: 09-2021
No related grants have been discovered for Nijoy John.