Libia Vega

Genotoxic effects of bistratene A on human lymphocytes

Publisher: Elsevier BV

Date: 03-1996

DOI: 10.1016/0165-1218(95)00097-6

Abstract: Bistratene A, a toxin isolated from the colonial ascidian Lissoclinum bistratum causes a decrease in mitotic index and retardation of lymphocyte proliferation kinetics when it is added at 48 h to 72-h human lymphocyte cultures. In the same cultures, the incidence of sister chromatid exchanges was not altered by this compound. We also observed an increase in the number of polyploid cells in the cultures, and alterations of the beta-tubulin organization by immunocytochemistry with an antibody against beta-tubulin. Bistratene A induces DNA damage in a dose-dependent fashion in leukocytes, as measured by the alkaline single cell gel electrophoresis assay. These results show that bistratene A interferes with microtubule assembly, is cytotoxic and cytostatic, and that it causes DNA damage.

National Autonomous University Of Mexico

Location: Mexico

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National Institute Of Environmental Health Sciences

Location: United States of America

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Centre Of Research And Advanced Studies Of The National Polytechnic Institute

Location: Mexico

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