ORCID Profile
0000-0002-1395-9814
Current Organisation
Deakin University - Geelong Campus at Waurn Ponds
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Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Materials Engineering | Physical Metallurgy | Materials Engineering Not Elsewhere Classified | Alloy Materials | Metals and Alloy Materials | Nanotechnology | Engineering And Technology Not Elsewhere Classified | Interdisciplinary Engineering not elsewhere classified | Manufacturing Engineering Not Elsewhere Classified | Materials Engineering not elsewhere classified | Manufacturing Engineering | Manufacturing Processes and Technologies (excl. Textiles) | Interdisciplinary Engineering Not Elsewhere Classified | Numerical Modelling and Mechanical Characterisation | Mechanical Engineering |
ANZSRC Socio-Economic Objective (SEO)
Metals (composites, coatings, bonding, etc.) | Structural metal products | Fabricated metal products not elsewhere classified | Semi-finished products | Sheet metal products | Expanding Knowledge in Technology | Other non-ferrous metals (e.g. copper,zinc) | Fabricated Metal Products not elsewhere classified | Structural Metal Products | Expanding Knowledge in Engineering | Expanding Knowledge in the Biological Sciences | Manufactured products not elsewhere classified | Basic Aluminium Products
Related Links
Publications
Mechanical strength and biocompatibility of ultrafine-grained commercial purity titanium
Publisher: Hindawi Limited
Date: 2013
DOI: 10.1155/2013/914764
Abstract: The effect of grain refinement of commercial purity titanium by equal channel angular pressing (ECAP) on its mechanical performance and bone tissue regeneration is reported. In vivo studies conducted on New Zealand white rabbits did not show an enhancement of biocompatibility of ECAP-modified titanium found earlier by in vitro testing. However, the observed combination of outstanding mechanical properties achieved by ECAP without a loss of biocompatibility suggests that this is a very promising processing route to bioimplant manufacturing. The study thus supports the expectation that commercial purity titanium modified by ECAP can be seen as an excellent candidate material for bone implants suitable for replacing conventional titanium alloy implants.
Deep drawing behaviour of ultrafine grained copper: modelling and experiment
Publisher: Springer Science and Business Media LLC
Date: 07-2009
Effect of bulk microstructure of commercially pure titanium on surface characteristics and fatigue properties after surface modification by sand blasting and acid-etching
Publisher: Elsevier BV
Date: 04-2016
DOI: 10.1016/J.JMBBM.2015.11.035
Abstract: Surface modification techniques are widely used to enhance the biological response to the implant materials. These techniques generally create a roughened surface, effectively increasing the surface area thus promoting cell adhesion. However, a negative side effect is a higher susceptibility of a roughened surface to failure due to the presence of multiple stress concentrators. The purpose of the study reported here was to examine the effects of surface modification by sand blasting and acid-etching (SLA) on the microstructure and fatigue performance of coarse-grained and ultrafine-grained (UFG) commercially pure titanium. Finer grain sizes, produced by equal channel angular pressing, resulted in lower values of surface roughness in SLA-processed material. This effect was associated with greater resistance of the UFG structure to plastic deformation. The fatigue properties of UFG Ti were found to be superior to those of coarse-grained Ti and conventional Ti-6Al-4V, both before and after SLA-treatment.
Effect of back pressure on the thermal stability of severely deformed copper
Publisher: IOP Publishing
Date: 08-08-2014
Mechanical twinning and texture evolution during asymmetric warm rolling of a high manganese steel
Publisher: Elsevier BV
Date: 09-2019
Poly (glycerol‐sebacate) bioelastomers: 2. Synthesis using B rabender P lasticoder® as a batch reactor
Publisher: Wiley
Date: 10-09-2015
DOI: 10.1002/APP.42852
The influence of back pressure on ECAP processed AA 6016: Modeling and experiment
Publisher: Elsevier BV
Date: 05-2007
Microstructures and properties of copper processed by equal channel angular extrusion for 1–16 passes
Publisher: Elsevier BV
Date: 09-2004
Effect of back pressure during equal-channel angular pressing on deformation-induced porosity in copper
Publisher: Elsevier BV
Date: 06-2013
Superplasticity in magnesium alloys by severe plastic deformation
Publisher: Elsevier
Date: 2012
The effect of warm equal channel angular extrusion on ductility and twinning in magnesium alloy ZK60
Publisher: Japan Institute of Metals
Date: 2004
An approach to the optimal design of rolling passes
Publisher: Elsevier BV
Date: 08-1997
Texture and Boundary Characteristics of Severely Deformed and Recrystallized Copper
Publisher: Trans Tech Publications Ltd.
Date: 15-10-2007
Hierarchical “As-Electrospun” Self-Assembled Fibrous Scaffolds Deconvolute Impacts of Chemically Defined Extracellular Matrix- and Cell Adhesion-Type Interactions on Stem Cell Haptokinesis
Publisher: American Chemical Society (ACS)
Date: 05-12-2017
Deformation field variations in equal channel angular extrusion due to back pressure
Publisher: Elsevier BV
Date: 05-2008
Production of Ti-6Al-4V billet through compaction of blended elemental powders by equal-channel angular pressing
Publisher: Elsevier BV
Date: 07-2012
Construction of extrusion limit diagram for AZ31 magnesium alloy by FE simulation
Publisher: Elsevier BV
Date: 03-2004
Analysis of texture and R value variations in asymmetric rolling of IF steel
Publisher: Elsevier BV
Date: 02-2012
High-throughput, deterministic single cell trapping and long-term clonal cell culture in microfluidic devices
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C4LC01176G
Abstract: In this paper, the design, development and validation of a novel high throughput microfluidic device enabling both the robust and rapid trapping of 100's to 1000's of single cells and their in situ clonal growth is described.
Processing routes leading to superplastic behaviour of magnesium alloy ZK60
Publisher: Elsevier BV
Date: 11-2005
A Comparison of Two Ovine Lumbar Intervertebral Disc Injury Models for the Evaluation and Development of Novel Regenerative Therapies
Publisher: SAGE Publications
Date: 10-06-2018
Non-equal channel angular pressing of aluminum alloy
Publisher: Elsevier BV
Date: 12-2009
Comparison of laboratory-scale and industrial-scale equal channel angular pressing of commercial purity titanium
Publisher: Elsevier BV
Date: 04-2015
Ultrafine-grained porous titanium and porous titanium/magnesium composites fabricated by space holder-enabled severe plastic deformation
Publisher: Elsevier BV
Date: 02-2016
DOI: 10.1016/J.MSEC.2015.10.070
Abstract: Compaction of powders by equal channel angular pressing (ECAP) using a novel space holder method was employed to fabricate metallic scaffolds with tuneable porosity. Porous Ti and Ti/Mg composites with 60% and 50% percolating porosity were fabricated using powder blends with two kinds of sacrificial space holders. The high compressive strength and good ductility of porous Ti and porous Ti/Mg obtained in this way are believed to be associated with the ultrafine grain structure of the pore walls. To understand this, a detailed electron microscopy investigation was employed to analyse the interface between Ti/Ti and Ti/Mg particles, the grain structures in Ti particles and the topography of pore surfaces. It was found that using the proposed compaction method, high quality bonding between particles was obtained. Comparing with other powder metallurgy methods to fabricate Ti with an open porous structure, where thermal energy supplied by a laser beam or high temperature sintering is essential, the ECAP process conducted at a relatively low temperature of 400°C was shown to produce unique properties.
Visualizing endocytic recycling and trafficking in live neurons by subdiffractional tracking of internalized molecules
Publisher: Springer Science and Business Media LLC
Date: 30-11-2017
Abstract: Our understanding of endocytic pathway dynamics is restricted by the diffraction limit of light microscopy. Although super-resolution techniques can overcome this issue, highly crowded cellular environments, such as nerve terminals, can also dramatically limit the tracking of multiple endocytic vesicles such as synaptic vesicles (SVs), which in turn restricts the analytical dissection of their discrete diffusional and transport states. We recently introduced a pulse-chase technique for subdiffractional tracking of internalized molecules (sdTIM) that allows the visualization of fluorescently tagged molecules trapped in in idual signaling endosomes and SVs in presynapses or axons with 30- to 50-nm localization precision. We originally developed this approach for tracking single molecules of botulinum neurotoxin type A, which undergoes activity-dependent internalization and retrograde transport in autophagosomes. This method was then adapted to localize the signaling endosomes containing cholera toxin subunit-B that undergo retrograde transport in axons and to track SVs in the crowded environment of hippoc al presynapses. We describe (i) the construction of a custom-made microfluidic device that enables control over neuronal orientation (ii) the 3D printing of a perfusion system for sdTIM experiments performed on glass-bottom dishes (iii) the dissection, culturing and transfection of hippoc al neurons in microfluidic devices and (iv) guidance on how to perform the pulse-chase experiments and data analysis. In addition, we describe the use of single-molecule-tracking analytical tools to reveal the average and the heterogeneous single-molecule mobility behaviors. We also discuss alternative reagents and equipment that can, in principle, be used for sdTIM experiments and describe how to adapt sdTIM to image nanocluster formation and/or tubulation in early endosomes during sorting events. The procedures described in this protocol take ∼1 week.
Fluorescent and Magnetic Mesoporous Hybrid Material: A Chemical and Biological Nanosensor for Hg2+ Ions
Publisher: Springer Science and Business Media LLC
Date: 25-02-2016
DOI: 10.1038/SREP21820
Abstract: We introduce “sense, track and separate” approach for the removal of Hg 2+ ion from aqueous media using highly ordered and magnetic mesoporous ferrosilicate nanocages functionalised with rhodamine fluorophore derivative. These functionalised materials offer both fluorescent and magnetic properties in a single system which help not only to selectively sense the Hg 2+ ions with a high precision but also adsorb and separate a significant amount of Hg 2+ ion in aqueous media. We demonstrate that the magnetic affinity of these materials, generated from the ultrafine γ-Fe 2 O 3 nanoparticles present inside the nanochannels of the support, can efficiently be used as a fluorescent tag to sense the Hg 2+ ions present in NIH3T3 fibroblasts live cells and to track the movement of the cells by external magnetic field monitored using confocal fluorescence microscopy. This simple approach of introducing multiple functions in the magnetic mesoporous materials raise the prospect of creating new advanced functional materials by fusing organic, inorganic and biomolecules to create advanced hybrid nanoporous materials which have a potential use not only for sensing and the separation of toxic metal ions but also for cell tracking in bio-separation and the drug delivery.
Extraordinary superplastic ductility of magnesium alloy ZK60
Publisher: Springer Science and Business Media LLC
Date: 06-2005
Abstract: Exceptionally high tensile ductility of commercial Mg alloy ZK60 is reported. It was achieved by equal channel angular pressing without any extra processing steps. The tensile ductility at 220 °C was 2040% and 1400% for the strain rates of 3 × 10 −4 s −1 and 3 × 10 −3 s −1 , respectively. The strain rate sensitivity of the flow stress exhibited a value slightly above 0.5, which is characteristic of superplastic deformation. The grain structure associated with this behavior was shown to be bi-modal with further separation in two fractions with different grain sizes within the small grain size population.
The effect of ball milling and equal channel angular pressing on the hydrogen absorption/desorption properties of Mg-4.95 wt% Zn-0.71 wt% Zr (ZK60) alloy
Publisher: Elsevier BV
Date: 2004
Effect of heat treatment on diffusion, internal friction, microstructure and mechanical properties of ultra-fine-grained nickel severely deformed by equal-channel angular pressing
Publisher: Elsevier BV
Date: 2015
Modeling the effect of primary and secondary twinning on texture evolution during severe plastic deformation of a twinning-induced plasticity steel
Publisher: MDPI AG
Date: 22-05-2018
DOI: 10.3390/MA11050863
An approach to preform design
Publisher: Elsevier BV
Date: 11-1995
Improving hydrogen storage properties of magnesium based alloys by equal channel angular pressing
Publisher: Elsevier BV
Date: 08-2009
Bimetallic copper-aluminium tube by severe plastic deformation
Publisher: Elsevier BV
Date: 06-2012
The influence of severe plastic deformation on the mechanical properties of AA6111
Publisher: Springer Science and Business Media LLC
Date: 05-2005
Evolution of nanoscale porosity during equal-channel angular pressing of titanium
Publisher: Elsevier BV
Date: 06-2009
Low-temperature compaction of Ti-6Al-4V powder using equal channel angular extrusion with back pressure
Publisher: Elsevier BV
Date: 08-2008
Targeted, Stimuli-Responsive Delivery of Plasmid DNA and miRNAs Using a Facile Self-Assembled Supramolecular Nanoparticle System
Publisher: American Chemical Society (ACS)
Date: 18-01-2018
Mechanical behavior of alloy AA6111 processed by severe plastic deformation: Modeling and experiment
Publisher: ASME International
Date: 15-02-2007
DOI: 10.1115/1.2744396
Abstract: An established dislocation density related, one-internal variable model was used, with some modifications, as a basis for modeling the mechanical response of aluminum alloy AA6111. In addition to conventional rolling, equal channel angular pressing (ECAP) was used to produce a wide range of grain sizes, down to the submicrometer scale. The s les were heat treated before and after both processes to optimize tensile ductility. Implementation of the model to uniaxial tensile response of the conventionally rolled and the ECAP processed materials confirmed its good predictive capability. The model was further used to formulate simple relations between true uniform strain and the constitutive parameters that allow reliable prediction of the uniform elongation.
Modeling the helical rolling of rods in a three-high mill
Publisher: Elsevier BV
Date: 08-1998
Architectured hybrid conductors: Aluminium with embedded copper helix
Publisher: Elsevier BV
Date: 02-2020
Effect of titanium surface topography on plasma deposition of antibacterial polymer coatings
Publisher: Elsevier BV
Date: 08-2020
Determinants of Zika virus host tropism uncovered by deep mutational scanning
Publisher: Springer Science and Business Media LLC
Date: 18-03-2019
Size effects in micro cup drawing
Publisher: Elsevier BV
Date: 07-2012
Thermal behavior of copper processed by ECAP with and without back pressure
Publisher: Elsevier BV
Date: 03-2015
Effect of Severe Plastic Deformation on the Conductivity and Strength of Copper-Clad Aluminium Conductors
Publisher: MDPI AG
Date: 09-2019
DOI: 10.3390/MET9090960
Abstract: Aluminium rods with different copper sheath thicknesses were processed by severe plastic deformation at room temperature and then annealed, to join the constituent metals and produce a nanocrystalline microstructure. A study of the effects of the deformation parameters, copper cladding thickness and annealing temperature on the electrical conductivity and hardness of the conductors is reported. It is shown that an interface forms between constituents because of intermixing in the course of severe shear deformation under high hydrostatic pressure and diffusion during the subsequent annealing. The effective conductivity of the aluminium copper-clad conductor dropped after deformation, but was recovered during annealing, especially during short annealing at 200 °C, to a level exceeding the theoretically predicted one. In addition, the annealing resulted in increased hardness at the interface and copper sheath.
Mechanical performance and cell response of pure titanium with ultrafine-grained structure produced by severe plastic deformation
Publisher: Elsevier
Date: 2018
Asymmetric Rolling of Interstitial-Free Steel Using Differential Roll Diameters. Part II: Microstructure and Annealing Effects
Publisher: Springer Science and Business Media LLC
Date: 28-08-2013
Effect of SPD Processing on the Strength and Conductivity of AA6061 Alloy
Publisher: Wiley
Date: 23-04-2019
Densification of Magnesium Particles by ECAP with a Back-Pressure
Publisher: Wiley
Date: 25-02-2005
Machining of coarse grained and ultra fine grained titanium
Publisher: Springer Science and Business Media LLC
Date: 23-02-2012
Tailoring LPSO phases in Mg–Y–Zn alloys to govern hydrogenation kinetics
Publisher: Springer Science and Business Media LLC
Date: 05-2023
DOI: 10.1007/S10853-023-08537-W
Abstract: A series of Mg–Y–Zn alloys with varying long-period stacking ordered (LPSO) phase fractions were prepared through control of alloy content, heat-treatment, and single-pass extrusion. The effect of LPSO phase volume fraction and microstructure refinement on the hydrogen absorption/desorption properties of ball-milled powders prepared from the extruded alloys was experimentally assessed. The hydrogen absorption and desorption kinetics scaled with the LPSO phase volume fraction, though the results of this study suggest that the scaling is not linear. Variations in the LPSO phase fraction and alloy content did not alter the (de)hydrogenation equilibrium pressure, indicating there is no significant change in thermodynamics of hydrogenation. Hydrogen absorption experiments on thin foils made from the extruded Mg–Y–Zn alloy with a high LPSO phase fraction demonstrated that the LPSO structures decompose into Mg phase, lamellar Mg/Mg–Zn structures and YH 2 particles at hydrogen partial pressures sufficient to form YH 2 . This study shows that the hydrogen absorption/desorption kinetics in the Mg–Y–Zn alloys can be controlled by tailoring the LPSO phases using conventional metallurgical techniques. Graphical Abstract
Tailoring biomaterial scaffolds for osteochondral repair
Publisher: Elsevier BV
Date: 05-2017
Effect of counterpressure during equal-channel angular pressing on nanoporosity formation in ultrafine-grained copper
Publisher: Pleiades Publishing Ltd
Date: 08-2011
Induction of Human iPSC-Derived Cardiomyocyte Proliferation Revealed by Combinatorial Screening in High Density Microbioreactor Arrays
Publisher: Springer Science and Business Media LLC
Date: 21-04-2016
DOI: 10.1038/SREP24637
Abstract: Inducing cardiomyocyte proliferation in post-mitotic adult heart tissue is attracting significant attention as a therapeutic strategy to regenerate the heart after injury. Model animal screens have identified several candidate signalling pathways, however, it remains unclear as to what extent these pathways can be exploited, either in idually or in combination, in the human system. The advent of human cardiac cells from directed differentiation of human pluripotent stem cells (hPSCs) now provides the ability to interrogate human cardiac biology in vitro , but it remains difficult with existing culture formats to simply and rapidly elucidate signalling pathway penetrance and interplay. To facilitate high-throughput combinatorial screening of candidate biologicals or factors driving relevant molecular pathways, we developed a high-density microbioreactor array (HDMA) – a microfluidic cell culture array containing 8100 culture chambers. We used HDMAs to combinatorially screen Wnt, Hedgehog, IGF and FGF pathway agonists. The Wnt activator CHIR99021 was identified as the most potent molecular inducer of human cardiomyocyte proliferation, inducing cell cycle activity marked by Ki67, and an increase in cardiomyocyte numbers compared to controls. The combination of human cardiomyocytes with the HDMA provides a versatile and rapid tool for stratifying combinations of factors for heart regeneration.
Single-cell transcriptome profiling reveals multicellular ecosystem of nucleus pulposus during degeneration progression
Publisher: Cold Spring Harbor Laboratory
Date: 26-05-2021
DOI: 10.1101/2021.05.25.445620
Abstract: Degeneration of the nucleus pulposus (NP) is a major contributor to intervertebral disc degeneration (IVDD) and low back pain. However, the underlying molecular complexity and cellular heterogeneity remain poorly understood. Here, we first reported a comprehensive single-cell resolution transcriptional landscape of human NP. Six novel human nucleus pulposus cell (NPCs) populations were identified by distinct molecular signatures. The potential functional differences among NPC subpopulations were analyzed at the single-cell level. Predictive genes, transcriptional factors, and signal pathways with respect to degeneration grades were analyzed. We reported that fibroNPCs, one of our identified subpopulations, might be a population for NP regeneration. CD90+NPCs were observed to be progenitor cells in degenerative NP tissues. NP-infiltrating immune cells comprise a previously unrecognized ersity of cell types, including granulocytic myeloid-derived suppressor cells (G-MDSCs). We uncovered CD11b, OLR1, and CD24 as surface markers of NP-derived G-MDSCs. The G-MDSCs were also found to be enriched in mildly degenerated (grade I and II) NP tissues compared to severely degenerated (grade III and IV) NP tissues. Their immunosuppressive function and protective effects for NPCs were revealed. Collectively, this study revealed the NPC type complexity and phenotypic characteristics in NP, providing new insights and clues for IVDD treatment.
Multivariate patterning of human pluripotent cells under perfusion reveals critical roles of induced paracrine factors in kidney organoid development
Publisher: American Association for the Advancement of Science (AAAS)
Date: 10-01-2020
Abstract: Multivariate patterning of perfused pluripotent cells reveals critical roles of induced paracrine factors in kidney organoids.
Enhancement of properties in cast Mg-Y-Zn rod processed by severe plastic deformation
Publisher: Elsevier BV
Date: 10-2014
Effect of the eutectic Al-(Ce,La) phase morphology on microstructure, mechanical properties, electrical conductivity and heat resistance of Al-4.5(Ce,La) alloy after SPD and subsequent annealing
Publisher: Elsevier BV
Date: 08-2019
Modelling ischemia-reperfusion injury (IRI) in vitro using metabolically matured induced pluripotent stem cell-derived cardiomyocytes
Publisher: AIP Publishing
Date: 20-03-2018
DOI: 10.1063/1.5000746
Abstract: Coronary intervention following ST-segment elevation myocardial infarction (STEMI) is the treatment of choice for reducing cardiomyocyte death but paradoxically leads to reperfusion injury. Pharmacological post-conditioning is an attractive approach to minimize Ischemia-Reperfusion Injury (IRI), but candidate drugs identified in IRI animal models have performed poorly in human clinical trials, highlighting the need for a human cell-based model of IRI. In this work, we show that when we imposed sequential hypoxia and reoxygenation episodes [mimicking the ischemia (I) and reperfusion (R) events] to immature human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs), they display significant hypoxia resistance and minimal cell death (∼5%). Metabolic maturation of hPSC-CMs for 8 days substantially increased their sensitivity to changes in oxygen concentration and led to up to ∼30% cell death post-hypoxia and reoxygenation. To mimic the known transient changes in the interstitial tissue microenvironment during an IRI event in vivo, we tested a new in vitro IRI model protocol that required glucose availability and lowering of media pH during the ischemic episode, resulting in a significant increase in cell death in vitro (∼60%). Finally, we confirm that in this new physiologically matched IRI in vitro model, pharmacological post-conditioning reduces reperfusion-induced hPSC-CM cell death by 50%. Our results indicate that in recapitulating key aspects of an in vivo IRI event, our in vitro model can serve as a useful method for the study of IRI and the validation and screening of human specific pharmacological post-conditioning drug candidates.
The Effect of a Small Copper Addition on the Electrical Conductivity of Aluminum
Publisher: Wiley
Date: 13-03-2020
A Mg-Al-Nd alloy produced via a powder metallurgical route
Publisher: Elsevier BV
Date: 07-2009
The calculation of stress-deformed state under non-isothermic plastic flow-the example of parallelepiped settling
Publisher: Elsevier BV
Date: 07-1992
Role of shear in interface formation of aluminium-steel multilayered composite sheets
Publisher: Elsevier BV
Date: 09-2017
The effect of grain refinement by warm equal channel angular extrusion on room temperature twinning in magnesium alloy ZK60
Publisher: Springer Science and Business Media LLC
Date: 04-2005
Driving Osteocytogenesis from Mesenchymal Stem Cells in Osteon-like Biomimetic Nanofibrous Scaffolds
Publisher: Cold Spring Harbor Laboratory
Date: 02-07-2022
DOI: 10.1101/2022.06.28.497866
Abstract: The repair of critical-sized bone defects, resulting from tumor resection, skeletal trauma or infection, remains a significant clinical problem. A potential solution is a tissue-engineered approach that utilises the combination of human mesenchymal stem cells (hMSCs) with synthetic biomaterial scaffolds, mimicking many of the biochemical and biophysical cues present within the native bone. Unfortunately, osteocyte cells, the orchestrators of bone maturation and homeostasis, are rarely produced within such MSC-seeded scaffolds, limiting the formation of true mature cortical bone from these synthetic implants. In this contribution, a bone morphogenic protein-6 (BMP6)-presenting osteon-like scaffolds based on electrospun poly(lactic-co-glycolic acid) (PLGA) fibrous scaffolds and poly(ethylene glycol) (PEG) based-hydrogels is reported. BMP6 peptide is shown to drive higher levels of SMAD signalling than the full-length protein counterpart. Osteon-mimetic scaffolds promoted the formation of osteocyte-like cells displaying multi-dendritic morphology and osteocyte-specific marker, E11/gp38 (E11), along with significant production of dentin matrix protein 1 (DMP1), confirming maturation of the ososteocyte-like cells. These results demonstrate that osteon-like scaffolds presenting chemo-topographical cues can drive the formation of mature osteocyte-like cells from hMSCs, without the need for osteogenic factor media supplements, providing a novel ex vivo production platform for osteocyte-like cells from human MSCs in cortical bone mimics.
Non-Conventional Fatigue Testing of CP Titanium for Medical Implants
Publisher: Wiley
Date: 02-05-2016
The equal channel angular pressing of magnesium and magnesium alloy powders
Publisher: Springer Science and Business Media LLC
Date: 08-2007
Enhanced Ductility due to Grain Refinement by Equal Channel Angular Extrusion in Automotive Aluminium Alloy 6016
Publisher: Trans Tech Publications Ltd.
Date: 15-01-2006
Bending Fatigue Testing of Commercial Purity Titanium for Dental Implants
Publisher: Wiley
Date: 04-05-2016
Enhanced superplasticity of magnesium alloy AZ31 obtained through equal-channel angular pressing with back-pressure
Publisher: Springer Science and Business Media LLC
Date: 12-2008
Optimization of upcycling of Ti-6Al-4V swarf
Publisher: Elsevier BV
Date: 05-2018
Effect of backpressure on structure and properties of AA5083 alloy processed by ECAP
Publisher: Elsevier BV
Date: 09-2004
A biocompatible thermoset polymer binder for Direct Ink Writing of porous titanium scaffolds for bone tissue engineering
Publisher: Elsevier BV
Date: 02-2019
DOI: 10.1016/J.MSEC.2018.10.033
Abstract: There is increasing demand for synthetic bone scaffolds for bone tissue engineering as they can counter issues such as potential harvesting morbidity and restrictions in donor sites which h er autologous bone grafts and address the potential for disease transmission in the case of allografts. Due to their excellent biocompatibility, titanium scaffolds have great potential as bone graft substitutes as they mimic the structure and properties of human cancellous bone. Here we report on a new thermoset bio-polymer which can act as a binder for Direct Ink Writing (DIW) of titanium artificial bone scaffolds. We demonstrate the use of the binder to manufacture porous titanium scaffolds with evenly distributed and highly interconnected porosity ideal for orthopaedic applications. Due to their porous structure, the scaffolds exhibit an effective Young's modulus similar to human cortical bone, alleviating undesirable stress-shielding effects, and possess superior strength. The biocompatibility of the scaffolds was investigated in vitro by cell viability and proliferation assays using human bone-marrow-derived Mesenchymal stem cells (hMSCs). The hMSCs displayed well-spread morphologies, well-organized F-actin and large vinculin complexes confirming their excellent biocompatibility. The vinculin regions had significantly larger Focal Adhesion (FA) area and equivalent FA numbers compared to that of tissue culture plate controls, showing that the scaffolds support cell viability and promote attachment. In conclusion, we have demonstrated the excellent potential of the thermoset bio-polymer as a Direct Ink Writing ready binder for manufacture of porous titanium scaffolds for hard tissue engineering.
ECAP with back pressure for optimum strength and ductility in aluminium alloy 6016. Part 2: Mechanical properties and texture
Publisher: Elsevier BV
Date: 05-2010
Effects of electric fields on human mesenchymal stem cell behaviour and morphology using a novel multichannel device
Publisher: Oxford University Press (OUP)
Date: 19-05-2015
DOI: 10.1039/C4IB00297K
Shear-induced, solid-state joining of Ti-6Al-4V and Ti17 titanium alloys
Publisher: Elsevier BV
Date: 11-2018
Random spectrum fatigue performance of severely plastically deformed titanium for implant dentistry applications
Publisher: Elsevier BV
Date: 07-2018
DOI: 10.1016/J.JMBBM.2018.04.012
Abstract: Severe plastic deformation (SPD) has long been known to confer superior mechanical properties for many metals and alloys. In the general field of biomedical devices, and dental implants in particular, the superior strength of SPD-processed commercially pure (CP) titanium, that may surpass that of the stronger Ti6Al4V alloy, has been associated with a superior fatigue resistance. Such a property would make those materials both biocompatible and strong alternatives to the currently used titanium alloy. However, the fatigue characterization reported so far in the literature relies on a very small s le size, thereby precluding any meaningful statistical analysis. This paper reports and compares systematic fatigue testing of various grades as-received and SPD processed Grade 4 CP-Ti using the recently developed random spectrum loading approach, in both air and 0.9% saline solution. The results of this study do not support the claim that the SPD process, albeit causing noticeable strengthening, confers any advantage to Grade 4 CP-Ti in terms of fatigue response.
ECAP with back pressure for optimum strength and ductility in aluminium alloy 6016. Part 1: Microstructure
Publisher: Elsevier BV
Date: 05-2010
Severe plastic deformation processes with friction induced shear
Publisher: Trans Tech Publications, Ltd.
Date: 12-2011
DOI: 10.4028/WWW.SCIENTIFIC.NET/MSF.667-669.25
Abstract: Among the known severe plastic deformation (SPD) techniques, there is a special group of processes involving friction-induced shear. One of the s le or work-piece dimensions used in such processes, namely the thickness, is much smaller than the other two dimensions. The well-known process of High Pressure Torsion (HPT) and the relatively new Cone-Cone (CC) method applied to thin conical s les fall into this category of SPD techniques. Wrought aluminium alloy 2124 was used to study the effect of CC processing on microstructure and mechanical properties. The influence of the processing parameters, including the rotation speed and thickness of the conical strip specimens on the microstructure and the mechanical properties of the CC-processed material was investigated.
Shear deformation with imposed hydrostatic pressure for enhanced compaction of powder
Publisher: Elsevier BV
Date: 05-2008
Improving sinterability of Ti-6Al-4V from blended elemental powders through equal channel angular pressing
Publisher: Elsevier BV
Date: 03-2013
The role of biomechanical factors in models of intervertebral disc degeneration across multiple length scales
Publisher: AIP Publishing
Date: 08-05-2023
DOI: 10.1063/5.0137698
Abstract: Low back pain is the leading cause of disability, producing a substantial socio-economic burden on healthcare systems worldwide. Intervertebral disc (IVD) degeneration is a primary cause of lower back pain, and while regenerative therapies aimed at full functional recovery of the disc have been developed in recent years, no commercially available, approved devices or therapies for the regeneration of the IVD currently exist. In the development of these new approaches, numerous models for mechanical stimulation and preclinical assessment, including in vitro cell studies using microfluidics, ex vivo organ studies coupled with bioreactors and mechanical testing rigs, and in vivo testing in a variety of large and small animals, have emerged. These approaches have provided different capabilities, certainly improving the preclinical evaluation of these regenerative therapies, but challenges within the research environment, and compromises relating to non-representative mechanical stimulation and unrealistic test conditions, remain to be resolved. In this review, insights into the ideal characteristics of a disc model for the testing of IVD regenerative approaches are first assessed. Key learnings from in vivo, ex vivo, and in vitro IVD models under mechanical loading stimulation to date are presented alongside the merits and limitations of each model based on the physiological resemblance to the human IVD environment (biological and mechanical) as well as the possible feedback and output measurements for each approach. When moving from simplified in vitro models to ex vivo and in vivo approaches, the complexity increases resulting in less controllable models but providing a better representation of the physiological environment. Although cost, time, and ethical constraints are dependent on each approach, they escalate with the model complexity. These constraints are discussed and weighted as part of the characteristics of each model.
Gradient structure produced by three roll planetary milling: Numerical simulation and microstructural observations
Publisher: Elsevier BV
Date: 07-2015
Influence of rolling asymmetry on the microstructure, texture and mechanical behavior of high-manganese twinning-induced plasticity steel
Publisher: Elsevier BV
Date: 2018
Kinematics of damage governed by severe plastic deformation
Publisher: Wiley
Date: 02-2002
Ecotourism enterprise and sustainable development in remote Indigenous communities in Australia
Publisher: Inderscience Publishers
Date: 2006
Improvement of die life by minimisation of damage accumulation and optimisation of preform design
Publisher: Elsevier BV
Date: 08-1998
Asymmetric Rolling of Interstitial-Free Steel Using Differential Roll Diameters. Part I: Mechanical Properties and Deformation Textures
Publisher: Springer Science and Business Media LLC
Date: 23-05-2013
Ultrafine-grained Al–5 wt.% Fe alloy processed by ECAP with backpressure
Publisher: Elsevier BV
Date: 09-2003
Microstructure and mechanical properties of Ti-15Zr alloy used as dental implant material
Publisher: Elsevier BV
Date: 09-2016
DOI: 10.1016/J.JMBBM.2016.05.008
Abstract: Ti-Zr alloys have recently started to receive a considerable amount of attention as promising materials for dental applications. This work compares mechanical properties of a new Ti-15Zr alloy to those of commercially pure titanium Grade4 in two surface conditions - machined and modified by sand-blasting and etching (SLA). As a result of significantly smaller grain size in the initial condition (1-2µm), the strength of Ti-15Zr alloy was found to be 10-15% higher than that of Grade4 titanium without reduction in the tensile elongation or compromising the fracture toughness. The fatigue endurance limit of the alloy was increased by around 30% (560MPa vs. 435MPa and 500MPa vs. 380MPa for machined and SLA-treated surfaces, respectively). Additional implant fatigue tests showed enhanced fatigue performance of Ti-15Zr over Ti-Grade4.
Substrate stiffness facilitates improved induced pluripotent stem cell production through modulation of both early and late phases of cell reprogramming
Publisher: Cold Spring Harbor Laboratory
Date: 03-2023
DOI: 10.1101/2023.02.27.530366
Abstract: Cell reprogramming involves time-intensive, costly processes that ultimately produce low numbers of reprogrammed cells of variable quality. By screening a range of polyacrylamide hydrogels (pAAm gels) of varying stiffness (1 kPA – 1.3 MPa) we found that a gel of medium stiffness significantly increases the overall number of reprogrammed cells by up to ten-fold with accelerated reprogramming kinetics, as compared to the standard Tissue Culture PolyStyrene (TCPS)-based protocol. We observe that though the gel improves both early and late phases of reprogramming, improvement in the late (reprogramming prone population maturation) phase is more pronounced and produces iPSCs having different characteristics and lower remnant transgene expression than those produced on TCPS. Comparative RNA-Seq analyses coupled with experimental validation reveals that modulation of Bone Morphogenic Protein (BMP) signalling by a novel reprogramming regulator, Phactr3, upregulated in the gel at an earliest time-point without the influence of transcription factors used for reprogramming, plays a crucial role in the improvement in the early reprogramming kinetics and overall reprogramming outcomes. This study provides new insights into the mechanism via which substrate stiffness modulates reprogramming kinetics and iPSC quality outcomes, opening new avenues for producing higher numbers of quality iPSCs or other reprogrammed cells at shorter timescales.
Removal of porosity in cast aluminium alloys by equal channel angular extrusion
Publisher: Trans Tech Publications, Ltd.
Date: 08-2003
Determination of lower-bound ductility for AZ31 magnesium alloy by use of the bulge specimens
Publisher: ASME International
Date: 08-12-2007
DOI: 10.1115/1.2744400
Abstract: Despite the high demand for industrial applications of magnesium, the forming technology for wrought magnesium alloys is not fully developed due to the limited ductility and high sensitivity to the processing parameters. The processing window for magnesium alloys could be significantly widened if the lower-bound ductility (LBD) for a range of stresses, temperature, and strain rates was known. LBD is the critical strain at the moment of fracture as a function of stress state and temperature. Measurements of LBD are normally performed by testing in a hyperbaric chamber, which is highly specialized, complex, and rare equipment. In this paper an alternative approach to determine LBD is demonstrated using wrought magnesium alloy AZ31 as an ex le. A series of compression tests of bulge specimens combined with finite element simulation of the tests were performed. The LBD diagram was then deduced by backward calculation.
Bimodal grain size distributions in UFG materials produced by SPD: Their evolution and effect on mechanical properties
Publisher: IOP Publishing
Date: 07-2010
Severe plastic deformation processes for thin samples
Publisher: Springer Science and Business Media LLC
Date: 27-03-2010
Influence of backpressure during ECAP on the monotonic and cyclic deformation behavior of AA5754 and Cu99.5
Publisher: Wiley
Date: 03-01-2011
Comparison of different extrusion methods for compaction of powders
Publisher: Trans Tech Publications, Ltd.
Date: 12-2011
DOI: 10.4028/WWW.SCIENTIFIC.NET/MSF.667-669.57
Abstract: Densification of metallic powders by means of extrusion is regarded as a very attractive processing technique that allows obtaining a high level of relative density of the compact. However, the uniformity of the relative density depends on that of strain distribution and on the processing parameters. Several variants of extrusion can be used for compaction of metal particulates, including the conventional extrusion (CE) and equal channel angular pressing (ECAP), often referred to as equal-channel angular extrusion. Each of these processes has certain advantages and drawbacks with respect to compaction. A comparative study of these two extrusion processes influencing the relative density of compacts has been conducted by numerical simulation using commercial finite element software DEFORM2D. The results have been validated by experiments with titanium and magnesium powders and chips.
The influence of post-ECAP annealing on the properties of ultrafine-grained 5005 aluminum alloy sheet
Publisher: Walter de Gruyter GmbH
Date: 04-2007
DOI: 10.3139/146.101466
Abstract: Equal Channel Angular Pressing is a well-known method of severe plastic deformation used to obtain ultrafine grained materials with significantly improved mechanical properties. To the present, Equal Channel Angular Pressing has essentially been restricted to the treatment of bulk billets of square or circular cross-section of limited size. A machine developed at Monash University allows the Equal Channel Angular Pressing of sheet material. In the present work, improvement in biaxial formability, associated textures and mechanical properties after one Equal Channel Angular Pressing pass followed by annealing for aluminium alloy 5005 were investigated. An optimal annealing treatment to obtain a good balance of properties was defined.
Asymmetric accumulative roll bonding of aluminium–titanium composite sheets
Publisher: Elsevier BV
Date: 08-2013
Investigation of damage accumulation using equal channel angular extrusion/drawing
Publisher: Elsevier
Date: 2000
Processing and properties of ultrafine-grain aluminum alloy 5005 sheet
Publisher: Springer Science and Business Media LLC
Date: 16-12-2006
Influence of equal channel angular pressing on high cycle fatigue behavior of ultrafine-grained iron: Role of anisotropy
Publisher: Elsevier BV
Date: 2018
Effect of Processing Parameters on the Magnetic Properties and Macrotexture of a Nd13.5Fe73.8Co6.7B5.6Ga0.4Alloy Processed by Equal Channel Angul
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 07-2016
Multicomponent materials from machining chips compacted by equal-channel angular pressing
Publisher: Springer Science and Business Media LLC
Date: 26-10-2014
Effects of synthetic biomacromolecule addition on the flow behavior of concentrated mesenchymal cell suspensions
Publisher: American Chemical Society (ACS)
Date: 12-12-2015
DOI: 10.1021/BM501481Y
Translational control of enzyme scavenger expression with toxin-induced micro RNA switches
Publisher: Springer Science and Business Media LLC
Date: 28-01-2021
DOI: 10.1038/S41598-021-81679-6
Abstract: Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic network to implement detoxification functionality. We employed a modular design strategy by engineering toxin-induced control of an enzyme scavenger. Our miRNA switch results show moderate synthetic expression control over a biologically active detoxification enzyme molecule, using an established design protocol. However, following a new design approach, we demonstrated an evolutionarily designed miRNA switch to more effectively activate enzyme activity than synthetically designed versions, allowing markedly improved extrinsic user-defined control with a toxin as inducer. Our straightforward new design approach is simple to implement and uses easily accessible web-based databases and prediction tools. The ability to exert control of toxicity demonstrates potential for modular detoxification systems that provide a pathway to new therapeutic and biocomputing applications.
Choroid plexus defects in Down syndrome brain organoids enhance neurotropism of SARS-CoV-2
Publisher: Cold Spring Harbor Laboratory
Date: 12-06-2023
DOI: 10.1101/2023.06.12.544552
Abstract: Why in iduals with Down Syndrome (DS, trisomy 21) are particularly susceptible to SARS CoV-2 induced neuropathology remains largely unclear. Since the choroid plexus (CP) performs important barrier and immune-interface functions, secretes the cerebrospinal fluid and strongly expresses the ACE2 receptor and the chromosome 21 encoded TMPRSS2 protease, we hypothesized that the CP could play a role in establishing SARS-CoV-2 infection in the brain. To investigate the role of the choroid plexus in SARS-CoV-2 central nervous system infection in DS, we established a new type of brain organoid from DS and isogenic euploid control iPSC that consists of a core of appropriately patterned functional cortical neuronal cell types that is surrounded by a patent and functional choroid plexus (CPCOs). Remarkably, DS-CPCOs not only recapitulated abnormal features of DS cortical development but also revealed defects in ciliogenesis and epithelial cell polarity of the developing choroid plexus. We next demonstrate that the choroid plexus layer facilitates SARS-CoV-2 replication and infection of cortical neuronal cells, and that this is increased in DS-CPCOs. We further show that inhibition of TMPRSS2 and Furin activity inhibits SARS-CoV-2 replication in DS CPCOs to the level observed in euploid organoids. We conclude that CPCOs are a useful model for dissecting the role of the choroid plexus in euploid and DS forebrain development and enables screening for therapeutics that can inhibit SARS-CoV-2 induced neuro-pathogenesis.
Strain gradient plasticity modelling of high-pressure torsion
Publisher: Elsevier BV
Date: 04-2008
Use of residual hydrogen to produce CP-Ti powder compacts for low temperature rolling
Publisher: Walter de Gruyter GmbH
Date: 12-2009
DOI: 10.3139/146.110226
Abstract: The present work investigates the optimal level of residual hydrogen in partially de-hydrogenated powder to produce CP-Ti plate compacts using ECAP with back pressure which are subsequently rolled at low temperature. A comparative study of the compaction of two TiH 2 powders and a CP-Ti powder, with particle sizes 150 μm, 50 μm and 45 μm respectively, has been carried out. The hydride powders have also been compacted in a partially de-hydrogenated state. The optimal level of residual hydrogen with respect to the density of the resulting compact and the associated mechanical properties has been defined. ECAP at 300°C produced compacts from these partially de-hydrogenated powders of 99.5 % theoretical density, while CP-Ti was compacted to almost full theoretical density under the same ECAP conditions. Therefore, the compaction of powder by ECAP does not benefit from temporary hydrogen alloying. These compacts then were rolled at temperatures ranging from room temperature to 500°C with an 80 % reduction in a single pass. Heat treatment after the rolling can modify the microstructure to improve the resulting mechanical properties and in this regard the temporary alloying with hydrogen has been observed to offer some significant benefits. It is shown that ECAP followed by low temperature rolling is a promising route to the batch production of fully dense CP-Ti wrought product from powder feedstock that avoids the need to subject the material to temperatures greater than 500°C. This low temperature route is expected to be efficient from an energy point of view and it also avoids the danger of interstitial contamination that accompanies most high temperature powder processing.
Approaches to the design and processing of novel titanium alloys
Publisher: Trans Tech Publications Ltd.
Date: 15-11-2007
Superplastic failure mode in ultrafine grained magnesium alloy AZ31
Publisher: Walter de Gruyter GmbH
Date: 04-2009
DOI: 10.3139/146.110067
Abstract: The paper addresses the failure mode of ultrafine grained magnesium alloy AZ31 under superplastic deformation conditions. The ultrafine grain structure and the resulting exceptional superplastic properties were produced by Equal Channel Angular Pressing with an imposed hydrostatic pressure conducted at a relatively low temperature of 150 °C. The mechanism of the superplastic ductility was studied by examining the fracture surfaces and internal cavities in superplastically deformed specimens by scanning electron microscopy. The presence of Mg 17 Al 12 particles was identified as the controlling microstructural feature giving rise to porosity formation and limiting tensile ductility. Fine details of the failure process depending on the particle and precipitate structure as well as textural effects were described.
Influence of the Strain History on TWIP Steel Deformation Mechanisms in the Deep-Drawing Process
Publisher: Springer Science and Business Media LLC
Date: 27-03-2018
Structure and Mechanical Properties of Asymmetrically Rolled IF Steel Sheet
Publisher: Trans Tech Publications, Ltd.
Date: 06-2010
DOI: 10.4028/WWW.SCIENTIFIC.NET/MSF.654-656.1255
Abstract: As-received hot-rolled 5.6 mm thick IF steel sheet was symmetrically/asymmetrically cold rolled at room temperature down to 1.9 mm. The asymmetric rolling was carried out in monotonic (an idle roll is always on the same side of the sheet) and reversal (the sheet was turned 180º around the rolling direction between passes) modes. Microstructure, texture and mechanical properties were analysed. The observed differences in structure and mechanical properties were modest, and therefore further investigation of the effects of other kinds of asymmetry is suggested.
Structure and mechanical property variations in Mg–Gd–Y–Zn–Zr alloy depending on its composition and processing conditions
Publisher: Informa UK Limited
Date: 04-03-2016
A comparison of continuous SPD processes for improving the mechanical properties of aluminum alloy 6111
Publisher: Springer Science and Business Media LLC
Date: 02-2009
Abstract: Microstructure evolution, mechanical properties, formability, and texture development were determined for AA6111 s les processed by asymmetric rolling (ASR) with different roll friction, velocity, or diameters, conventional rolling (CR), and equal-channel-angular pressing (ECAP). Highly elongated or sheared grain structures were developed during ASR/CR and ECAP, respectively. ASR led to improved r -values and formability compared with CR primarily as a result of the development of moderate shear-texture components analogous to those developed during ECAP of billet material. ASR based on different roll diameters gave the best combination of strength, ductility, and formability.
Combinatorial presentation of cartilage-inspired peptides on nanopatterned surfaces enables directed differentiation of human mesenchymal stem cells towards distinct articular chondrogenic phenotypes
Publisher: Elsevier BV
Date: 07-2019
Five Piconewtons: The Difference between Osteogenic and Adipogenic Fate Choice in Human Mesenchymal Stem Cells.
Publisher: American Chemical Society (ACS)
Date: 03-10-2019
Abstract: The ability of mesenchymal stem cells to sense nanoscale variations in extracellular matrix (ECM) compositions in their local microenvironment is crucial to their survival and their fate however, the underlying molecular mechanisms defining how such fates are temporally modulated remain poorly understood. In this work, we have utilized self-assembled block copolymer surfaces to present nanodomains of an adhesive peptide found in many ECM proteins at different lateral spacings (from 30 to 60 nm) and studied the temporal response (2 h to 14 days) of human mesenchymal stem cells (hMSCs) using a panel of real-time localization and activity biosensors. Our findings revealed that within the first 4 to 24 h postadhesion and spreading, hMSCs on smaller nanodomain spacings recruit more activated FAK and Src proteins to produce larger, longer-lived, and increased numbers of focal adhesions (FAs). The adhesions formed on smaller nanospacings rapidly recruit higher amounts of nonmuscle myosin IIA and vinculin and experience tension forces (by >5 pN/FA) significantly higher than those observed on larger nanodomain spacings. The transmission of higher levels of tension into the cytoskeleton at short times was accompanied by higher Rac1, cytosolic β-catenin, and nuclear localization of YAP/TAZ and RUNX2, which together biased the commitment of hMSCs to an osteogenic fate. This investigation provides mechanistic insights to confirm that smaller lateral spacings of adhesive nanodomains alter hMSC mechanosensing and biases mechanotransduction at short times
Strain localisation patterns under equal-channel angular pressing
Publisher: Elsevier BV
Date: 2009
Structure and properties of Mg-Al-Ca alloy after severe plastic deformation
Publisher: Trans Tech Publications, Ltd.
Date: 06-2008
DOI: 10.4028/WWW.SCIENTIFIC.NET/MSF.584-586.559
Abstract: Severe plastic deformation of a Mg-Al-Ca alloy resulted in different types of grain structure. High pressure torsion (HPT) was shown to lead to the formation of a nanocrystalline structure with a grain size of 100-200 nm, while equal channel angular pressing (ECAP) produced ultrafine grained (UFG) or submicrocrystalline (SMC) structures, depending on the ECAP temperature. An UFG structure with a grain size of 2-5 -m was formed at 300°C, as distinct from a finer SMC structure with a grain size of 300-800 nm formed at a lower temperature (220°C). The possibility of increasing the strength of the alloy in the UFG condition by a factor of 1.5-2, combined with a reasonable level of ductility and enhanced functional properties was thus demonstrated. ECAP of annealed Mg-Al-Ca with the formation of UFG structure was shown to lead to increased yield strength (by a factor of 2) and enhanced tensile ductility (by a factor of 3).
A model of grain fragmentation based on lattice curvature
Publisher: Elsevier BV
Date: 03-2010
Gradient Structures in Thin‐Walled Metallic Tubes Produced by Continuous High Pressure Tube Shearing Process
Publisher: Wiley
Date: 03-07-2017
Enhancement of electrical conductivity in aluminum single crystals by boron treatment in solid state
Publisher: Springer Science and Business Media LLC
Date: 10-2019
Accelerated stem cell attachment to ultrafine grained titanium
Publisher: Elsevier BV
Date: 02-2011
DOI: 10.1016/J.ACTBIO.2010.09.033
Abstract: Commercial purity titanium with an average grain size in the low sub-micron range was produced by equal channel angular pressing (ECAP). Attachment of human bone marrow-derived mesenchymal stem cells (hMSCs) to the surface of conventional coarse grained and ECAP-modified titanium was studied. It was demonstrated that the attachment and spreading of hMSCs in the initial stages (up to 24h) of culture was enhanced by grain refinement. Surface characterization by a range of techniques showed that the main factor responsible for the observed acceleration of hMSC attachment and spreading on titanium due to grain refinement in the bulk is the attendant changes in surface topography on the nanoscale. These results indicate that, in addition to its superior mechanical properties, ECAP-modified titanium possesses improved biocompatibility, which makes it to a potent candidate for applications in medical implants.
Damage mechanics for the fracture prediction of metal forming tools
Publisher: Springer Science and Business Media LLC
Date: 2000
Rapamycin mitigates Valproic Acid-induced teratogenicity in human and animal models by suppressing AP-1-mediated senescence
Publisher: Cold Spring Harbor Laboratory
Date: 31-08-2023
Size effect on the tensile strength of fine-grained copper
Publisher: Elsevier BV
Date: 12-2008
Sintering and biocompatibility of blended elemental Ti-xNb alloys
Publisher: Elsevier BV
Date: 04-2020
Fatigue crack growth in ultrafine grained aluminium alloy
Publisher: Trans Tech Publications, Ltd.
Date: 06-2011
DOI: 10.4028/WWW.SCIENTIFIC.NET/MSF.690.254
Abstract: The microstructure, fatigue crack growth behaviour and hardness of ultra fine grained 6061 aluminium alloy obtained by equal angle channel processing was studied. ECAP resulted in significant grain refinement down to the sub micron level and corresponding increase in hardness. Results point to a similar fatigue threshold stress intensity range and fatigue crack growth rates for 1, 2, 4 and 6 passes of ECAP.
Equal-channel angular pressing and annealing of a twinning-induced plasticity steel: Microstructure, texture, and mechanical properties
Publisher: Elsevier BV
Date: 04-2016
The role of back-pressure in equal channel angular extrusion
Publisher: Springer Science and Business Media LLC
Date: 2005
Asymmetric Rolling of Interstitial-Free Steel Using One Idle Roll
Publisher: Springer Science and Business Media LLC
Date: 30-11-2011
The influence of strain hardening on steel erosion wear studied by analytical modelling of impeller testing
Publisher: Informa UK Limited
Date: 27-03-2019
Microstructure and mechanical properties of LPSO phase dominant Mg85.8Y7.1Zn7.1 and Mg85.8Y7.1Ni7.1 alloys
Publisher: Elsevier BV
Date: 04-2017
Pushing the Limits of High Throughput PET-RAFT Polymerization
Publisher: American Chemical Society (ACS)
Date: 20-09-2018
Erratum
Publisher: Informa UK Limited
Date: 31-03-2016
Fostering self-authorship through work integrated learning in university tourism programs: A missed opportunity?
Publisher: Informa UK Limited
Date: 14-11-2019
Tropomyosin Tpm 2.1 loss induces glioblastoma spreading in soft brain-like environments
Publisher: Springer Science and Business Media LLC
Date: 09-12-2018
DOI: 10.1007/S11060-018-03049-Z
Abstract: The brain is a very soft tissue. Glioblastoma (GBM) brain tumours are highly infiltrative into the surrounding healthy brain tissue and invasion mechanisms that have been defined using rigid substrates therefore may not apply to GBM dissemination. GBMs characteristically lose expression of the high molecular weight tropomyosins, a class of actin-associating proteins and essential regulators of the actin stress fibres and focal adhesions that underpin cell migration on rigid substrates. Here, we investigated how loss of the high molecular weight tropomyosins affects GBM on soft matrices that recapitulate the biomechanical architecture of the brain. We find that Tpm 2.1 is down-regulated in GBM grown on soft substrates. We demonstrate that Tpm 2.1 depletion by siRNA induces cell spreading and elongation in soft 3D hydrogels, irrespective of matrix composition. Tpm 1.7, a second high molecular weight tropomyosin is also down-regulated when cells are cultured on soft brain-like surfaces and we show that effects of this isoform are matrix dependent, with Tpm 1.7 inducing cell rounding in 3D collagen gels. Finally, we show that the absence of Tpm 2.1 from primary patient-derived GBMs correlates with elongated, mesenchymal invasion. We propose that Tpm 2.1 down-regulation facilitates GBM colonisation of the soft brain environment. This specialisation of the GBM actin cytoskeleton organisation that is highly suited to the soft brain-like environment may provide novel therapeutic targets for arresting GBM invasion.
Damage evolution under severe plastic deformation
Publisher: Springer Science and Business Media LLC
Date: 2002
Self-Organizing 3D Human Choroid Plexus-Ventricle-Cortical Organoids
Publisher: Cold Spring Harbor Laboratory
Date: 10-2020
DOI: 10.1101/2020.09.30.321554
Abstract: Both the choroid plexus (CP) and the cortex are derived from the rostral neural tube during early embryonic development. In addition to producing CSF, the CP secretes essential factors that orchestrate cortical development and later neurogenesis. Previous brain modeling efforts with human pluripotent stem cells (hPSCs) generated either cortical or CP tissues in 3D culture. Here, we used hPSC-derived neuroectodermal cells, the building blocks of the anterior body, to simultaneously generate CP that forms ventricles and cortical cells in organoids (CVCOs), which can be maintained as 3D organoid cultures. Large scale culture revealed reproducibility of the protocol independent of cell lines, clones or batches. CVCOs contain mature and functional CP that projects multiple cilia into the ventricle-like fluid filled cysts and is in direct contact with appropriately patterned cortical cells. CVCOs thus recapitulate key features of developing forebrain structures observed in in vivo and constitute a useful for dissecting the role of CP in human forebrain development in health and disease.
Differential response of patient-derived primary glioblastoma cells to environmental stiffness
Publisher: Springer Science and Business Media LLC
Date: 21-03-2016
DOI: 10.1038/SREP23353
Abstract: The ability of cancer cells to sense external mechanical forces has emerged as a significant factor in the promotion of cancer invasion. Currently there are conflicting reports in the literature with regard to whether glioblastoma (GBM) brain cancer cell migration and invasion is rigidity-sensitive. In order to address this question we have compared the rigidity-response of primary patient-derived GBM lines. Cells were plated on polyacrylamide gels of defined rigidity that reflect the ersity of the brain tissue mechanical environment and cell morphology and migration were analysed by time-lapse microscopy. Invasiveness was assessed in multicellular spheroids embedded in 3D matrigel cultures. Our data reveal a range of rigidity-dependent responses between the patient-derived cell lines, from reduced migration on the most compliant tissue stiffness to those that are insensitive to substrate rigidity and are equally migratory irrespective of the underlying substrate stiffness. Notably, the rigidity-insensitive GBM cells show the greatest invasive capacity in soft 3D matrigel cultures. Collectively our data confirm both rigidity-dependent and independent behaviour in primary GBM patient-derived cells.
Optimization of Strength‐Electrical Conductivity Properties in Al–2Fe Alloy by Severe Plastic Deformation and Heat Treatment
Publisher: Wiley
Date: 20-11-2017
A damage accumulation model for complex strain paths: Prediction of ductile failure in metals
Publisher: Elsevier BV
Date: 11-2009
Ti-6Al-4V billet produced by compaction of BE powders using equal-channel angular pressing
Publisher: Trans Tech Publications, Ltd.
Date: 08-2012
DOI: 10.4028/WWW.SCIENTIFIC.NET/KEM.520.301
Abstract: In the present study, ECAP compaction was used to produce Ti-6Al-4V billet from CP Ti powder mixed with two different Al-V master alloys. It was demonstrated that ECAP at 400 °C with applied back-pressure of about 210 MPa permits consolidation of the powder mixtures to relative green densities as high as 99.3 %. A combination of temperature, high hydrostatic pressure and plastic shear deformation gave rise to excellent green densities and good compositional homogeneity due to enhanced self-diffusion. It was shown that the temperature of sintering required after direct compaction of BE powders can be reduced by 150-250°C if compaction is conducted by ECAP with back-pressure. This becomes possible due to high green density and the presence of a large number of fast diffusion paths associated with dislocations and grain boundaries. It is suggested that the ECAP-based processing route may offer a significant saving of production cost.
Tailoring One-Pass Asymmetric Rolling of Extra Low Carbon Steel for Shear Texture and Recrystallization
Publisher: MDPI AG
Date: 15-06-2019
DOI: 10.3390/MA12121935
Abstract: Systematic single pass rolling experiments were carried out at room temperature on extra low carbon steel by varying the roll diameter ratio between 1:1 to 1:2 and thickness reduction per pass in the range of 20–75%. The aim of this study was to define the conditions under which the rolling texture can transit into a shear texture. The consequences for grain fragmentation, tensile strength, recrystallization texture, and grain growth kinetics were also studied. It was found that in a certain range of thickness reduction per pass and asymmetric ratio, an effective rotation towards the shear texture takes place in conventional rolling. The value of the shear coefficient factor (shear strain rate/rolling strain rate) in asymmetric rolling depends on the selection of thickness reduction per pass. The measured value of shear coefficient was found to be independent of the number of passes used in asymmetric rolling. The consequence of arising shear textures is an acceleration of grain fragmentation. After rapid heat treatment, both tensile strength and recrystallization kinetics of asymmetric rolled sheets showed merits over the conventional rolling. Only the evolved Goss orientation from asymmetric conditions of deformation shows higher stability than any other preferred shear texture components after complete recrystallization.
The influence of temporary hydrogenation on ECAP formability and low cycle fatigue life of CP titanium
Publisher: Elsevier BV
Date: 02-2011
Enhancement of mechanical and electrical properties of Al-RE alloys by optimizing rare-earth concentration and thermo-mechanical treatment
Publisher: Elsevier BV
Date: 05-2018
Prior thermo-mechanical processing to modify structure and properties of severely deformed low carbon steel
Publisher: IOP Publishing
Date: 08-08-2014
Enhanced superplasticity in a magnesium alloy processed by equal-channel angular pressing with a back-pressure
Publisher: Wiley
Date: 05-2008
Microstructure and electrical conductivity of aluminium/steel bimetallic rods processed by severe plastic deformation
Publisher: Springer Science and Business Media LLC
Date: 25-04-2016
Microfluidic Screening Reveals Heparan Sulfate Enhances Human Mesenchymal Stem Cell Growth by Modulating Fibroblast Growth Factor-2 Transport
Publisher: Oxford University Press (OUP)
Date: 16-02-2017
DOI: 10.1002/SCTM.16-0343
Abstract: Cost-effective expansion of human mesenchymal stem/stromal cells (hMSCs) remains a key challenge for their widespread clinical deployment. Fibroblast growth factor-2 (FGF-2) is a key hMSC mitogen often supplemented to increase hMSC growth rates. However, hMSCs also produce endogenous FGF-2, which critically interacts with cell surface heparan sulfate (HS). We assessed the interplay of FGF-2 with a heparan sulfate variant (HS8) engineered to bind FGF-2 and potentiate its activity. Bone marrow-derived hMSCs were screened in perfused microbioreactor arrays (MBAs), showing that HS8 (50 μg/ml) increased hMSC proliferation and cell number after 3 days, with an effect equivalent to FGF-2 (50 ng/ml). In combination, the effects of HS8 and FGF-2 were additive. Differential cell responses, from upstream to downstream culture chambers under constant flow of media in the MBA, provided insights into modulation of FGF-2 transport by HS8. HS8 treatment induced proliferation mainly in the downstream chambers, suggesting a requirement for endogenous FGF-2 accumulation, whereas responses to FGF-2 occurred primarily in the upstream chambers. Adding HS8 along with FGF-2, however, maximized the range of FGF-2 effectiveness. Measurements of FGF-2 in static cultures then revealed that this was because HS8 caused increased endogenous FGF-2 production and liberated FGF-2 from the cell surface into the supernatant. HS8 also sustained levels of supplemented FGF-2 available over 3 days. These results suggest HS8 enhances hMSC proliferation and expansion by leveraging endogenous FGF-2 production and maximizing the effect of supplemented FGF-2. This is an exciting strategy for cost-effective expansion of hMSCs.
Microstructure evolution and fatigue behavior of 2124 aluminum processed by ECAE with back pressure
Publisher: Elsevier BV
Date: 06-2006
Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB
Publisher: Springer Science and Business Media LLC
Date: 30-09-2016
DOI: 10.1038/NCOMMS12976
Abstract: Axonal retrograde transport of signalling endosomes from the nerve terminal to the soma underpins survival. As each signalling endosome carries a quantal amount of activated receptors, we hypothesized that it is the frequency of endosomes reaching the soma that determines the scale of the trophic signal. Here we show that upregulating synaptic activity markedly increased the flux of plasma membrane-derived retrograde endosomes (labelled using cholera toxin subunit-B: CTB) in hippoc al neurons cultured in microfluidic devices, and live Drosophila larval motor neurons. Electron and super-resolution microscopy analyses revealed that the fast-moving sub-diffraction-limited CTB carriers contained the TrkB neurotrophin receptor, transiently activated by synaptic activity in a BDNF-independent manner. Pharmacological and genetic inhibition of TrkB activation selectively prevented the coupling between synaptic activity and the retrograde flux of signalling endosomes. TrkB activity therefore controls the encoding of synaptic activity experienced by nerve terminals, digitalized as the flux of retrogradely transported signalling endosomes.
Correction to: Influence of the Strain History on TWIP Steel Deformation Mechanisms in the Deep-Drawing Process
Publisher: Springer Science and Business Media LLC
Date: 20-08-2018
Accelerated growth of preosteoblastic cells on ultrafine grained titanium
Publisher: Wiley
Date: 31-07-2009
DOI: 10.1002/JBM.A.32174
Abstract: This work is part of a general effort to demonstrate the effect of the bulk microstructure of titanium as a model bone implant material on viability of osteoblasts (bone-forming cells). The objective of this work was to study the proliferation of preosteoblastic MC3T3-E1 cells extracted from mice embryos on commercial purity titanium substrates. Two distinct states of titanium were considered: as-received material with an average grain size of 4.5 microm and that processed by equal channel angular pressing (ECAP), with an average grain size of 200 nm. We report the first results of an in vitro study into the effect of this extreme grain refinement on viability and proliferation of MC3T3-E1 cells. By means of MTT assays it was demonstrated that ECAP processing of titanium enhances MC3T3-E1 culture proliferation in a spectacular way. This finding suggests that bone implants made from ECAP processed titanium may promote bone tissue growth.
The focal adhesion targeting domain of p130Cas confers a mechanosensing function
Publisher: The Company of Biologists
Date: 2017
DOI: 10.1242/JCS.192930
Abstract: The Cas family of focal adhesion proteins contain a highly conserved C-terminal focal adhesion targeting (FAT) domain. To determine the role of the FAT domain we compared wildtype exogenous NEDD9 with a hybrid construct in which the NEDD9 FAT domain is exchanged for the p130Cas FAT domain. Fluorescence recovery after photobleaching (FRAP) revealed significantly slowed exchange of the fusion protein at focal adhesions and significantly slower 2D migration. No differences were detected in cell stiffness measured with Atomic Force Microscopy (AFM) and cell adhesion forces measured with a magnetic tweezer device. Thus the slowed migration was not due to changes in cell stiffness or adhesion strength. Analysis of cell migration on surfaces of increasing rigidity revealed a striking reduction of cell motility in cells expressing the p130Cas FAT domain. The p130Cas FAT domain induced rigidity-dependent tyrosine phosphorylation of the NEDD9 substrate domain. This in turn reduced post-translational cleavage of NEDD9 which we show inhibits NEDD9-induced migration. Collectively, our data therefore suggest that the p130Cas FAT domain uniquely confers mechanosensing function.
Enhancement of Mechanical and Electrical Properties in Al 6101 Alloy by Severe Shear Strain under Hydrostatic Pressure
Publisher: Wiley
Date: 20-09-2018
Equal channel angular pressing with rotating shear plane to produce hybrid materials with helical architecture of constituents
Publisher: Springer Science and Business Media LLC
Date: 11-09-2017
DOI: 10.1557/JMR.2017.339
Single-Cell Transcriptome Profiling Reveals Multicellular Ecosystem of Nucleus Pulposus during Degeneration Progression.
Publisher: Wiley
Date: 26-11-2021
Abstract: Although degeneration of the nucleus pulposus (NP) is a major contributor to intervertebral disc degeneration (IVDD) and low back pain, the underlying molecular complexity and cellular heterogeneity remain poorly understood. Here, a comprehensive single‐cell resolution transcript landscape of human NP is reported. Six novel human NP cells (NPCs) populations are identified by their distinct molecular signatures. The potential functional differences among NPC subpopulations are analyzed. Predictive transcripts, transcriptional factors, and signal pathways with respect to degeneration grades are explored. It is reported that fibroNPCs is the subpopulation for end‐stage degeneration. CD90+NPCs are observed to be progenitor cells in degenerative NP tissues. NP‐infiltrating immune cells comprise a previously unrecognized ersity of cell types, including granulocytic myeloid‐derived suppressor cells (G‐MDSCs). Integrin α M (CD11b) and oxidized low density lipoprotein receptor 1 (OLR1) as surface markers of NP‐derived G‐MDSCs are uncovered. The G‐MDSCs are found to be enriched in mildly degenerated (grade II and III) NP tissues compared to severely degenerated (grade IV and V) NP tissues. Their immunosuppressive function and alleviation effects on NPCs’ matrix degradation are revealed in vitro. Collectively, this study reveals the NPC‐type complexity and phenotypic characteristics in NP, thereby providing new insights and clues for IVDD treatment.
Interface Formation in Copper-Steel Multilayered Sheets under Severe Shear Strain
Publisher: Wiley
Date: 22-03-2019
Possibility to Predict Extrusion Die Incidental Fracture by Finite Element Simulation
Publisher: Wiley
Date: 21-12-2016
Design of LPSO Phases in Mg-Y-Ni Alloys to Impact Hydrogenation Kinetics
Publisher: MDPI AG
Date: 10-09-2023
Self-authorship development through tourism education: rethinking the outcomes of work-integrated learning
Publisher: Edward Elgar Publishing
Date: 27-01-2017
Deconstructing replicative senescence heterogeneity of human mesenchymal stem cells at single cell resolution reveals therapeutically targetable senescent cell sub-populations
Publisher: Cold Spring Harbor Laboratory
Date: 27-01-2022
DOI: 10.1101/2022.01.24.476823
Abstract: Cellular senescence is characterised by a state of permanent cell cycle arrest. It is accompanied by often variable release of the so-called senescence-associated secretory phenotype (SASP) factors, and occurs in response to a variety of triggers such as persistent DNA damage, telomere dysfunction, or oncogene activation. While cellular senescence is a recognised driver of organismal ageing, the extent of heterogeneity within and between different senescent cell populations remains largely unclear. Elucidating the drivers and extent of variability in cellular senescence states is important for discovering novel targeted seno-therapeutics and for overcoming cell expansion constraints in the cell therapy industry. Here we combine cell biological and single cell RNA-sequencing approaches to investigate heterogeneity of replicative senescence in human ESC-derived mesenchymal stem cells (esMSCs) as MSCs are the cell type of choice for the majority of current stem cell therapies and senescence of MSC is a recognized driver of organismal ageing. Our data identify three senescent subpopulations in the senescing esMSC population that differ in SASP, oncogene expression, and escape from senescence. Uncovering and defining this heterogeneity of senescence states in cultured human esMSCs allowed us to identify potential drug targets that may delay the emergence of senescent MSCs in vitro and perhaps in vivo in the future.
Hydrogenation effect on microstructure and mechanical properties of Mg-Gd-Y-Zn-Zr alloys
Publisher: Elsevier BV
Date: 03-2018
Prediction of ductile failure in CP-Titanium as criterion of SPD process design
Publisher: Elsevier BV
Date: 03-2016
Compaction of Ti-6Al-4V powder by ECAE with back-pressure
Publisher: Trans Tech Publications Ltd.
Date: 15-11-2007
Gradient plasticity constitutive model reflecting the ultrafine micro-structure scale: the case of severely deformed copper
Publisher: Elsevier BV
Date: 04-2005
Processing and properties of ultrafine-grain aluminium alloy 6111 sheet
Publisher: Elsevier BV
Date: 05-2008
Combined effect of grain refinement and surface modification of pure titanium on the attachment of mesenchymal stem cells and osteoblast-like SaOS-2 cells
Publisher: Elsevier BV
Date: 02-2017
DOI: 10.1016/J.MSEC.2016.10.035
Abstract: Surface modification is an important step in production of medical implants. Surface roughening creates additional surface area to enhance the bonding between the implant and the bone. Recent research provided a means to alter the microstructure of titanium by severe plastic deformation (SPD) in order to increase its strength, and thereby reduce the size of the implants (specifically, their diameter). The purpose of the present study was to examine the effect of bulk microstructure of commercially pure titanium with coarse-grained (CG) and ultrafine-grained (UFG) bulk structure on the surface state of these materials after surface modification by sand blasting and acid etching (SLA). It was shown that SLA-modified surface characteristics, in particular, roughness, chemistry, and wettability, were affected by prior SPD processing. Additionally, biocompatibility of UFG titanium was examined using osteosarcoma cell line SaOS-2 and primary human adipose-derived mesenchymal stem cell (adMSC) cultures. Enhanced cell viability as well as increased matrix mineralization during osteogenic differentiation of MSCs on the surface of ultrafine-grained titanium was shown.
Investigation of Ductility and Damage Accumulation by Two Stage Deformation Using Ecae/Ecad and the Tensile Test
Publisher: Springer Netherlands
Date: 2000
The effect of hydrogenation on the ECAP compaction of Ti–6Al–4V powder and the mechanical properties of compacts
Publisher: Elsevier BV
Date: 07-2009
The Positive Role of Back-Pressure in Equal Channel Angular Extrusion
Publisher: Trans Tech Publications Ltd.
Date: 15-01-2006
Developing a taxonomy of 'award-winning' restaurants-what are they actually?
Publisher: Informa UK Limited
Date: 25-04-2011
Severe plastic deformation of a TWIP steel
Publisher: Elsevier BV
Date: 2014
Bacterial attachment on sub-nanometrically smooth titanium substrata
Publisher: Informa UK Limited
Date: 17-01-2013
DOI: 10.1080/08927014.2012.757697
Abstract: Despite the volume of work that has been conducted on the topic, the role of surface topography in mediating bacterial cell adhesion is not well understood. The primary reason for this lack of understanding is the relatively limited extent of topographical characterisation employed in many studies. In the present study, the topographies of three sub-nanometrically smooth titanium (Ti) surfaces were comprehensively characterised, using nine in idual parameters that together describe the height, shape and distribution of their surface features. This topographical analysis was then correlated with the adhesion behaviour of the pathogenic bacteria Staphylococcus aureus and Pseudomonas aeruginosa, in an effort to understand the role played by each aspect of surface architecture in influencing bacterial attachment. While P. aeruginosa was largely unable to adhere to any of the three sub-nanometrically smooth Ti surfaces, the extent of S. aureus cell attachment was found to be greater on surfaces with higher average, RMS and maximum roughness and higher surface areas. The cells also attached in greater numbers to surfaces that had shorter autocorrelation lengths and skewness values that approached zero, indicating a preference for less ordered surfaces with peak heights and valley depths evenly distributed around the mean plane. Across the sub-nanometrically smooth range of surfaces tested, it was shown that S. aureus more easily attached to surfaces with larger features that were evenly distributed between peaks and valleys, with higher levels of randomness. This study demonstrated that the traditionally employed litudinal roughness parameters are not the only determinants of bacterial adhesion, and that spatial parameters can also be used to predict the extent of attachment.
A double chamber rotating bioreactor for enhanced tubular tissue generation from human mesenchymal stem cells: a promising tool for vascular tissue regeneration
Publisher: Hindawi Limited
Date: 05-04-2017
DOI: 10.1002/TERM.2341
Abstract: Cardiovascular diseases represent a major global health burden, with high rates of mortality and morbidity. Autologous grafts are commonly used to replace damaged or failing blood vessels however, such approaches are h ered by the scarcity of suitable graft tissue, donor site morbidity and poor long-term stability. Tissue engineering has been investigated as a means by which exogenous vessel grafts can be produced, with varying levels of success to date, a result of mismatched mechanical properties of these vessel substitutes and inadequate ex vivo vessel tissue genesis. In this work, we describe the development of a novel multifunctional dual-phase (air/aqueous) bioreactor, designed to both rotate and perfuse small-diameter tubular scaffolds and encourage enhanced tissue genesis throughout such scaffolds. Within this novel dynamic culture system, an elastomeric nanofibrous, microporous composite tubular scaffold, composed of poly(caprolactone) and acrylated poly(lactide-co-trimethylene-carbonate) and with mechanical properties approaching those of native vessels, was seeded with human mesenchymal stem cells (hMSCs) and cultured for up to 14 days in inductive (smooth muscle) media. This scaffold/bioreactor combination provided a dynamic culture environment that enhanced (compared with static controls) scaffold colonization, cell growth, extracellular matrix deposition and in situ differentiation of the hMSCs into mature smooth muscle cells, representing a concrete step towards our goal of creating a mature ex vivo vascular tissue for implantation. Copyright © 2016 John Wiley & Sons, Ltd.
Development of an in-process UV-crosslinked, electrospun PCL/aPLA-co-TMC composite polymer for tubular tissue engineering applications
Publisher: Elsevier BV
Date: 05-2016
Texture evolution during micro-drawing of ultrafine grained copper
Publisher: Elsevier BV
Date: 07-2010
On the influence of the misorientation of grains, grain size and boundary volume on the strength and ductility of ultrafine grained Cu
Publisher: Springer Science and Business Media LLC
Date: 2004
Abstract: The influence of the microstructure and the misorientation relationship of grains on mechanical properties is investigated in specimens of ultrafine-grained copper processed by equal channel angular extrusion (ECAE) route B c for 1, 4 and 12 passes. XRD texture analyses have shown that the major texture component is developed during the first pass of ECAE, and remains approximately constant with greater number of passes. EBSD measurements indicate that the majority of grain boundaries are still of low angle ( °), while after four and twelve passes more than 50 % of all boundaries are high angle ones. TEM analyses have shown that the microstructure evolves from microbands and elongated cells towards a more equiaxed homogenous microstructure. On the microscale observed by TEM the degree of misorientation among subgrains/cells increases and the width of boundaries decreases while the cell/subgrain size remains approximately constant as the number of passes increases. The mechanical properties show a saturation level with a maximum in the yield stress and UTS after 4 passes. The strength of the material decreases between the fourth and the twelfth passes and the uniform elongation increases. It is suggested that the increase in ductility (and decrease in strength) is associated with the decrease in width of boundaries leading to an increase in the mean free path of dislocations.
Effect of Backpressure on the Structure and Properties of Al-Based Alloys Processed by ECAP
Publisher: Trans Tech Publications, Ltd.
Date: 08-2003
Optimisation of interface formation by shear inclination: Example of aluminium-cooper hybrid produced by ECAP with back-pressure
Publisher: Elsevier BV
Date: 05-2018
Poverty, indigenous culture and ecotourism in remote Australia
Publisher: Springer Science and Business Media LLC
Date: 21-05-2007
Self-organised nanoarchitecture of titanium surfaces influences the attachment of Staphylococcus aureus and Pseudomonas aeruginosa bacteria
Publisher: Springer Science and Business Media LLC
Date: 17-04-2015
DOI: 10.1007/S00253-015-6572-7
Abstract: The surface nanotopography and architecture of medical implant devices are important factors that can control the extent of bacterial attachment. The ability to prevent bacterial attachment substantially reduces the possibility of a patient receiving an implant contracting an implant-borne infection. We now demonstrated that two bacterial strains, Staphylococcus aureus and Pseudomonas aeruginosa, exhibited different attachment affinities towards two types of molecularly smooth titanium surfaces each possessing a different nanoarchitecture. It was found that the attachment of S. aureus cells was not restricted on surfaces that had an average roughness (S a) less than 0.5 nm. In contrast, P. aeruginosa cells were found to be unable to colonise surfaces possessing an average roughness below 1 nm, unless sharp nanoprotrusions of approximately 20 nm in size and spaced 35.0 nm apart were present. It is postulated that the enhanced attachment of P. aeruginosa onto the surfaces possessing these nanoprotrusions was facilitated by the ability of the cell membrane to stretch over the tips of the nanoprotrusions as confirmed through computer simulation, together with a concomitant increase in the level of extracellular polymeric substance (EPS) being produced by the bacterial cells.
Development of Asymmetric Rolling for the Better Control over Structure and Mechanical Properties in IF Steel
Publisher: Trans Tech Publications, Ltd.
Date: 2012
DOI: 10.4028/WWW.SCIENTIFIC.NET/MSF.706-709.2788
Abstract: In the present study, the effects of kinematic and geometric asymmetries in rolling during multi-pass processing of IF steel are examined. The theoretical investigation by final element simulations and experimental investigations by means of electron-backscatter diffraction analysis and tensile tests suggest that asymmetric rolling increases the total imposed strain compared to symmetric rolling, and largely re-distributes the strain components due to additional shear. This enhances the intensity of grain refinement, strengthens and tilts crystallographic orientations, and increases mechanical strength. The effect is highest in the asymmetric rolling with differential roll diameters.
Texture evolution and grain refinement of ultrafine-grained copper during micro-extrusion
Publisher: Informa UK Limited
Date: 04-10-2010
Related Organisations
Deakin University - Geelong Campus At Waurn Ponds
Location: Australia
CSIRO Australian Manufacturing And Materials Precinct
Location: Australia
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