ORCID Profile
0000-0003-3396-2911
Current Organisation
NSW Department of Primary Industries
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Publisher: Elsevier BV
Date: 09-2020
Publisher: Elsevier BV
Date: 2013
DOI: 10.1016/J.FOODCHEM.2012.08.035
Abstract: Foods with elevated levels of resistant starch (RS) may have beneficial effects on human health. Pasta was enriched with commercial resistant starches (RSII, Hi Maize™ 1043 RSIII, Novelose 330™) at 10%, 20% and 50% substitution of semolina for RSII and 10% and 20% for RSIII and compared with pasta made from 100% durum wheat semolina to investigate technological, sensory, in vitro starch digestibility and structural properties. The resultant RS content of pasta increased from 1.9% to ∼21% and was not reduced on cooking. Significantly, the results indicate that 10% and 20% RSII and RSIII substitution of semolina had no significant effects on pasta cooking loss, texture and sensory properties, with only a minimal reduction in pasta yellowness. Both RS types lowered the extent of in vitro starch hydrolysis compared to that of control pasta. X-ray diffraction and small-angle scattering verified the incorporation of RS and, compared to the control s le, identified enhanced crystallinity and a changed molecular arrangement following digestion. These results can be contrasted with the negative impact on pasta resulting from substitution with equivalent amounts of more traditional dietary fibre such as bran. The study suggests that these RS-containing formulations may be ideal sources for the preparation of pasta with reduced starch digestibility.
Publisher: Elsevier BV
Date: 03-2021
Publisher: Elsevier BV
Date: 2021
Publisher: Scientific Societies
Date: 2018
Publisher: MDPI AG
Date: 28-05-2020
DOI: 10.3390/FOODS9060693
Abstract: Resistant starch (RS) in foods has positive benefits for potentially alleviating lifestyle diseases. RS is correlated positively with starch amylose content. This study aimed to see what level of amylose in durum wheat is needed to lower pasta GI. The silencing of starch synthases IIa (SSIIa) and starch branching enzymes IIa (SBEIIa), key genes involved in starch biosynthesis, in durum wheat cultivar Svevo was performed and spaghetti was prepared and evaluated. The SSIIa and SBEIIa mutants have a 28% and 74% increase in amylose and a 2.8- and 35-fold increase in RS, respectively. Cooked pasta was softer, with higher cooking loss but lower stickiness compared to Svevo spaghetti, and with acceptable appearance and colour. In vitro starch digestion extent (area under the digestion curve) was decreased in both mutants, but much more in SBEIIa, while in vivo GI was only significantly reduced from 50 to 38 in SBEIIa. This is the first study of the glycaemic response of spaghetti prepared from SBEIIa and SSIIa durum wheat mutants. Overall pasta quality was acceptable in both mutants but the SBEIIa mutation provides a clear glycaemic benefit and would be much more appealing than wholemeal spaghetti. We suggest a minimum RS content in spaghetti of ~7% is needed to lower GI which corresponded to an amylose content of ~58%.
Publisher: Wiley
Date: 03-2021
DOI: 10.1002/CCHE.10408
Publisher: Wiley
Date: 2018
DOI: 10.1002/CCHE.10007
Publisher: Wiley
Date: 31-05-2023
DOI: 10.1002/CCHE.10562
Abstract: Durum wheat breeding commenced in Australia in the 1930s by the New South Wales Department of Primary Industries (NSW DPI), Australia. Dural was developed in 1956 from a cross between North African landraces. Since then, another 20 varieties have been released in the following six decades by NSW DPI and the University of Adelaide. These were evaluated for agronomic and detailed quality traits including pasta‐making quality over three seasons. This study aimed to quantify progress achieved in Australian durum breeding for yield, quality, and some agronomic traits since the release of Dural. A demonstrated grain yield improvement at the rate of 27.8 kg ha −1 year −1 up to 2017 has been achieved when generally maintaining low screenings and high thousand grain weight. The recent varieties are medium early in their maturity relative to Dural and possess improved lodging tolerance which has resulted in better adaptation to seasonal conditions under dryland cultivation, and also, adaptation to high input irrigated cropping. Breeding has also resulted in higher technological quality, specifically, improved semolina yellow color, higher dough strength, improved pasta brightness and yellowness but with slightly declining grain protein content. Strong genetic gain was observed in semolina color traits, namely, reduction in a * and increase in b *. The progress in Australian durum breeding over the last six decades compares well to the progress achieved in other countries for yield, agronomic, and quality traits. The improvements in yield and quality are expected to continue with future developments focussing on adaptation to meet the changing climate and improving tolerance to a major disease, crown rot, while maintaining high technological quality. This is the first study quantifying the progress made in Australian durum breeding efforts and the first study that includes pasta‐making quality traits from an historical breeding perspective.
Publisher: Elsevier BV
Date: 11-2023
Publisher: Wiley
Date: 17-04-2018
DOI: 10.1002/CCHE.10048
Publisher: Wiley
Date: 21-12-2020
DOI: 10.1002/CCHE.10246
Publisher: Wiley
Date: 29-06-2016
Publisher: Elsevier BV
Date: 11-2016
DOI: 10.1016/J.CARBPOL.2016.06.016
Abstract: The roles that the compact structure and proteins in pasta play in retarding evolution of starch molecular structure during in vitro digestion are explored, using four types of cooked s les: whole pasta, pasta powder, semolina (with proteins) and extracted starch without proteins. These were subjected to in vitro digestion with porcine α-amylase, collecting s les at different times and characterizing the weight distribution of branched starch molecules using size-exclusion chromatography. Measurement of α-amylase activity showed that a protein (or proteins) from semolina or pasta powder interacted with α-amylase, causing reduced enzymatic activity and retarding digestion of branched starch molecules with hydrodynamic radius (Rh) 100nm.
Publisher: Wiley
Date: 28-05-2014
DOI: 10.1002/JSFA.6723
Abstract: The incorporation of fibres, whether insoluble or soluble, in durum wheat pasta negatively impacts desirable end-use properties, especially if incorporated in significant amounts. Fibres can disrupt the starch-protein matrix of the dough during pasta preparation and can also often swell more readily with water than starch, competing with the starch for water during dough development. Similar degrees of substitution with different fibres gave markedly different impacts on firmness, stickiness, cooking loss and sensory attributes, suggesting that results obtained for one fibre cannot readily be generalized to other fibres. The in vitro starch digestibility of the pastas was significantly reduced when resistant starch, β-glucan-enriched flour, carboxymethyl cellulose or guar gum was incorporated but increased when pollard or inulin was added. In many instances, different sources of the same fibre gave dramatically different impacts on the properties of cooked durum wheat pasta.
Publisher: Elsevier
Date: 2014
Publisher: Elsevier BV
Date: 12-2017
Publisher: Wiley
Date: 08-02-2021
DOI: 10.1111/IJFS.14984
Abstract: Spaghetti commonly made with durum wheat, typically lacks essential amino acids and with low fibre. Legumes offer potential to improve these properties and lupin is a good choice because it is cheaper with fewer antinutritional compounds than other legumes. Spaghetti was prepared with 0%, 5%, 17% and 30% lupin protein isolate (LPI) using both single‐screw (SSEP) and twin‐screw extrusion (TSEP). LPI increased protein up to 129%, reduced cooking time, water absorption and cooked firmness while stickiness and cooking loss were increased, especially using TSEP. LPI made the dried pasta more red and yellow and decreased brightness. The percentage of starch digested under in vitro conditions was reduced using 17%LPI with TSEP and 30%LPI with SSEP compared vs. control. Microscopy revealed changes in structure by LPI which may explain impacts on technological properties and digestion. Inclusion of LPI in pasta represents a potential approach for a low‐cost improvement of nutritional value of spaghetti and potentially reducing its starch digestibility.
Publisher: CSIRO Publishing
Date: 2014
DOI: 10.1071/CP13305
Abstract: Durum wheat (Triticum turgidum ssp. durum) is typically used to produce pasta. In some parts of the world, it is used to make bread but with inferior loaf volume and texture compared with common wheat bread. This study describes the effect on technological properties of pasta and bread made from durum wheat cv. Svevo (recurrent parent (S), HMW-GS null, 7+8) and two isogenic genotypes carrying pairs of additional subunits 5+10 (S 5+10) or 2+12 (S 2+12), normally present at the Glu-D1 locus in bread wheat. The semolina was re-ground to flour, mixed in various proportions with bakers flour and used to prepare loaves. The dough properties of the S 5+10 line were markedly different from Svevo, having over-strong, stable dough, low wet gluten and elasticity S 2+12 also displayed stronger dough. Pasta prepared from these genotypes showed lower cooked firmness (adjusted for protein differences), ranked Svevo S 5+10 = S 2+12. There were no other differences in pasta cooking quality. Bread loaf volume and loaf score decreased as more bakers flour was replaced by durum flour, but the decline varied with the genetic material and dosage. The greatest reduction in loaf volume occurred using S 5+10 and the least with S 2+12, which was similar to Svevo. Bake score was reduced with S 5+10 only. The best loaf was made using Svevo. This work shows that it is possible to manipulate the processing properties of pasta and durum–bread-wheat blends by altering the glutenin subunit composition. This represents an efficient tool to finely manipulate gluten quality in durum wheat.
Publisher: Scientific Societies
Date: 2019
Publisher: MDPI AG
Date: 04-01-2022
Abstract: Pasta made from durum wheat is a widely consumed worldwide and is a healthy and convenient food. In the last two decades, there has been much research effort into improving the nutritional value of pasta by inclusion of nonconventional ingredients due to the demand by health-conscious consumers for functional foods. These ingredients can affect the technological properties of the pasta, but their health impacts are not always measured rather inferred. This review provides an overview of pasta made from durum wheat where the semolina is substituted in part with a range of ingredients (barley fractions, dietary fibre sources, fish ingredients, herbs, inulin, resistant starches, legumes, vegetables and protein extracts). Impacts on pasta technological properties and in vitro measures of phytonutrient enhancement or changes to starch digestion are included. Emphasis is on the literature that provides clinical or animal trial data on the health benefits of the functional pasta.
Publisher: Hapres
Date: 2020
Publisher: MDPI AG
Date: 16-11-2022
Abstract: Durum wheat is widely used in various products, including long and short dried pasta, fresh and sheeted pasta, couscous, bulgur and baked bread [...]
Publisher: CSIRO Publishing
Date: 2014
DOI: 10.1071/CP13431
Abstract: To extend the production base of durum wheat in Australia, field trials were conducted on seven registered durum varieties across four seasons and six sites in locations where irrigation was supplied during crop growth. The purpose was to determine if the quality of the grain produced met the requirements for good milling and pasta-making quality and to understand the genotype, environment and their interaction in affecting yield and technological quality of the grain and derived pasta. High grain yields and grain protein were obtained, producing large grain weights, low screenings and low percentage of hard vitreous kernels. Yellow colour of semolina and pasta was reduced marginally but dough and other pasta technological characteristics were similar to typical dryland durum production, with some exceptions. Varieties were identified with potential for production under irrigation.
Publisher: Wiley
Date: 14-02-2019
DOI: 10.1002/CCHE.10137
Publisher: Elsevier BV
Date: 12-2015
DOI: 10.1016/J.FOODCHEM.2015.05.032
Abstract: The aim of the present study is to characterise the influence of gluten structure on the kinetics of starch hydrolysis in pasta. Spaghetti and powdered pasta were prepared from three different cultivars of durum semolina, and starch was also purified from each cultivar. Digestion kinetic parameters were obtained through logarithm-of-slope analysis, allowing identification of sequential digestion steps. Purified starch and semolina were digested following a single first-order rate constant, while pasta and powdered pasta followed two sequential first-order rate constants. Rate coefficients were altered by pepsin hydrolysis. Confocal microscopy revealed that, following cooking, starch granules were completely swollen for starch, semolina and pasta powder s les. In pasta, they were completely swollen in the external regions, partially swollen in the intermediate region and almost intact in the pasta strand centre. Gluten entrapment accounts for sequential kinetic steps in starch digestion of pasta the compact microstructure of pasta also reduces digestion rates.
Publisher: Elsevier BV
Date: 09-2022
Publisher: Wiley
Date: 19-02-2019
DOI: 10.1002/CCHE.10136
Publisher: MDPI AG
Date: 24-10-2022
Abstract: The cereal disease Fusarium crown rot (FCR), caused by the fungal pathogen Fusarium pseudograminearum, is a worldwide major constraint to winter cereal production but especially in Australia’s northern grain’s region (NGR) of NSW and Queensland. Conventionally, FCR induced yield penalties are associated with semi-arid water-limited conditions during flowering and grain-filling. In this study, yield penalties associated with FCR infection were found to be significant under both adequate and above average water conditions which has implication for global wheat production in more favorable environments. This research was conducted to understand the impact of FCR on water availability, yield and grain quality in high protein bread and durum wheat varieties in controlled environment and replicated field experiments across three locations in the NGR over a two-year period. Under controlled conditions, FCR infection significantly decreased water use by 7.5% with an associated yield reduction of 9.5% irrespective of water treatment. Above average rainfall was experienced across all field experimental sites in both 2020 and 2021 growing seasons. The field studies demonstrated a decrease in water use of upwards of 23% at some sites and significant yield penalties across all cultivars of up to 18.4% in natural rainfed scenarios to still 13.2% with further supplementary irrigation.
Publisher: MDPI AG
Date: 24-02-2021
Abstract: Wheat bran is a conventional by-product of the wheat milling industry mainly used for animal feed. It is a rich and inexpensive source of phytonutrients, so is in demand for fibre-rich food products but creates quality issues when incorporated into bread. The purpose of this study was to characterize the physicochemical properties and phytochemical composition of different size durum bran fractions and show how they impact bread quality. Durum wheat (Triticum durum Desf.) was milled to create a coarse bran fraction (CB), which was further ground into a finer fraction (FB) which was sieved using four screens with apertures 425, 315, 250, 180, and µm to create a particle size range of 1497 to 115 µm. All fractions contained phytosterol with highest in the 180 and FB, while total phenolic acids and antioxidant capacity was highest in CB and 425. Use of the fractions in a leavened common wheat (T. aestivum L.) bread formula at 10% incorporation negatively impacted bread loaf volume, colour, and texture compared to standard loaves, with CB having the least impact. Results suggest that to combine the highest phytochemical content with minimal impact on bread quality, bran particle size should be considered, with CB being the best choice.
Publisher: MDPI AG
Date: 24-01-2023
Abstract: The cereal disease Fusarium crown rot (FCR), caused by the fungal pathogen Fusarium pseudograminearum (Fp), is a major constraint to cereal production worldwide. Nitrogen (N) fertilizer is estimated to be approximately 30% of the input costs for grain growers in Australia and is the primary driver of yield and grain protein levels. When targeting high yield and protein, generous nitrogen fertilizer applications are thought to result in large biomass production, which exacerbates FCR severity, reducing grain yield and quality. This research was undertaken to investigate the effect of temporal N availability in high-protein bread and durum wheat varieties on FCR severity. Laboratory and controlled environment experiments assessed the relationship between FCR and N at a mechanistic and plant level. An in vitro study demonstrated an increase in Fp mycelial growth under increased N availability, especially when N was supplied as urea compared with ammonium nitrate. Similarly, under controlled environmental conditions, increased soil N availability promoted FCR severity within infected plants. Stem N transfer efficiency was significantly decreased under FCR infection in both bread and durum wheat varieties by 4.5% and 10.2%, respectively. This new research demonstrates that FCR not only decreases yield and grain quality but appears to have previously unrecognised detrimental impacts on nitrogen-use efficiency in wheat. This indicates that the current impact of losses from FCR may also decrease N-use inefficiencies, as well as yield and quality penalties. An improved understanding of the interactions and restrictions of FCR infection may allow growers to better manage the disease through manipulation of the soil’s temporal N availability.
Publisher: Wiley
Date: 07-2016
Publisher: MDPI AG
Date: 15-11-2022
Abstract: Durum semolina spaghetti is known to have a low-moderate glycaemic index but the impact of various processing variables during the manufacture and cooking of pasta does affect pasta structure and potentially could alter starch digestion. In this study, several process variables were investigated to see if they can impact the in vitro starch digestion in spaghetti while also monitoring the pasta’s technological quality. Cooking time had a large impact on pasta starch digestion and reducing cooking from fully cooked to al dente and using pasta of very high protein content (17%), reduced starch digestion extent. The semolina particle size distribution used to prepare pasta impacted pasta quality and starch digestion to a small extent indicating a finer semolina particle size ( µm) may promote a more compact structure and help to reduce starch digestion. The addition of a structural enzyme, Transglutaminase in the pasta formulae improved overcooking tolerance in low protein pasta comparable to high protein pasta with no other significant effects and had no effect on starch digestion over a wide protein range (8.6–17%). While cold storage of cooked pasta was expected to increase retrograded starch, the increase in resistant starch was minor (37%) with no consequent improvement in the extent of starch digestion. Varying three extrusion parameters (die temperature, die pressure, extrusion speed) impacted pasta technological quality but not the extent of starch digestion. Results suggest the potential to subtly manipulate the starch digestion of pasta through some processing procedures.
Publisher: Wiley
Date: 22-01-2018
DOI: 10.1002/CCHE.10027
Publisher: Wiley
Date: 11-04-2023
DOI: 10.1002/CCHE.10667
Abstract: While there are a few studies examining the impact of isolated starch A and B granule preparations on cereal end product quality, there is limited information about the effect of genotypic variation in B granule content in starch on pasta technological properties and pasta in vitro starch digestion. Crosses were made between a durum variety Yallaroi and a low and high B‐granule dicoccoides accession and the resulting population evaluated in field trials for starch B‐granule content (S‐BG) and selections made for more detailed analysis. Genotypes were grouped based on semolina B granule contents of 24.5%−26.7%, 31.7%−37.1%, and 43.9%−45.1% for analysis purposes. Preliminary field data indicated three “43.9−45.1%” B‐granule genotypes had lower grain and test weight and one of these, 156,224*2, a very high semolina Buhler mill yield. Pasta made from the genotypes produced acceptable pasta but there was no association between S‐BG and pasta properties. To overcome protein composition differences between genotypes, starch was isolated and reconstituted flours and pasta prepared. Starch from the same “43.9−45.1%” group all showed RVA profiles with lower final viscosities and setback and displayed higher gelatinization onset and peak temperatures than the “24.5−26.7%” group. Pasta made from reconstituted flours showed similar pasta quality and in vitro starch digestion properties. The data suggests no relationship between percentage of B‐granules and both pasta quality and starch digestion for the range and populations studied (S‐BG, 22%−44%). This information provides new insights into the role varying S‐BG content in pasta cooking properties, texture and nutritional characteristics and could provide some direction to breeders to consider breeding for very low or very high B‐granule content.
Publisher: MDPI AG
Date: 19-10-2023
Publisher: Wiley
Date: 29-09-2016
Publisher: Wiley
Date: 20-04-2020
DOI: 10.1002/CCHE.10285
Publisher: CSIRO Publishing
Date: 2023
DOI: 10.1071/CP23091
Publisher: Elsevier BV
Date: 05-2019
Publisher: Wiley
Date: 09-2013
Publisher: Scientific Societies
Date: 09-2017
Publisher: Elsevier BV
Date: 05-2015
Publisher: MDPI AG
Date: 29-05-2023
DOI: 10.20944/PREPRINTS202305.1952.V1
Abstract: Abstract: In Australia adoption of reduced tillage and stubble retention cropping systems to conserve soil moisture by growers has seen an increase in the prevalence of the disease Fusarium crown rot (FCR) caused by the stubble-borne fungal pathogen Fusarium pseudograminearum. Durum wheat is particularly susceptible to FCR expressing significant yield and quality losses in the presence of infection. Increasing rates of nitrogen (N) application at sowing exacerbates FCR. However, to achieve the desired grain protein and quality suited to pasta manufacture, N application is necessary and this creates a dilemma for growers. The purpose of this study was to investigate the effects of FCR infection in the presence of different N fertiliser application rates in durum wheat varieties on yield and technological quality. Two durum varieties were evaluated at the same location over two seasons (2020 and 2021). These seasons were characterised by being wetter than normal and showed different responses to FCR and N application. In general, the 2021 season showed better responses to applied N with no impact of FCR. The FCR inoculation while it resulted in significant infection, it had no impacts on yield or grain quality in 2021 while in 2020 yield was reduced and only gluten index was reduced. Jandaroi was found to hold its kernel vitreosity at all protein levels obtaining the premium grade while DBA Lillaroi did not and would suffer downgrade if N application was insufficient. However, higher N application rates needed to achieve 12% + protein saw a reduction in dough strength with Jandaroi holding its dough strength much better than DBA Lillaroi. This was related to the lower glutenin/gliadin (Gli/Glu) ratio in response to applied N at sowing in Jandaroi which helped retain kernels with high vitreousness. This suggests genetics plays an important role in a genotype response to N fertilisation and should be considered when selecting a variety where higher premium grades are desirable.
Publisher: American Society of Hematology
Date: 29-01-2015
DOI: 10.1182/BLOOD-2014-04-571372
Abstract: PTEN phosphatase activity, independent of other PTEN functions, is required to prevent T-cell lymphoma.
Publisher: MDPI AG
Date: 24-05-2022
Abstract: Although durum wheat (Triticum durum L. ssp. durum Desf.) has traditionally been used to make a range of food products, its use has been restricted due to the absence of the D-genome glutenin proteins, the relatively low variability in starch composition, and its very hard grain texture. This review focuses on the manipulation of the starch and protein composition and modification of the hardness of durum wheat in order to improve its technological and nutritional value and expand its utilization for application to a wider number of end products. Starch is composed of amylopectin and amylose in a 3:1 ratio, and their manipulation has been explored for achieving starch with modified composition. In particular, silencing of the genes involved in amylose and amylopectin synthesis has made it possible to isolate durum wheat lines with amylose content varying from 2–3% up to 75%. This has created opportunities for new products with different properties and enhanced nutritional value. Durum-made bread has generally inferior quality to bread made from common wheat. Attempts to introduce the Glu-D1 subunits 1Dx5 + 1Dy10 and 1Dx2 + 1Dy12 produced stronger dough, but the former produced excessively strong, inelastic doughs, and loaf volume was either inferior or not affected. In contrast, the 1Dx2 + 1Dy12 sometimes improved bread loaf volume (LV) depending on the glutenin subunit background of the genotype receiving these genes. Further breeding and selection are needed to improve the dough extensibility to allow higher LV and better texture. The versatility of durum wheat has been greatly expanded with the creation of soft-textured durum via non-GMO introgression means. This soft durum mills like soft hexaploid wheat and has similar baking properties. The pasta quality is also not diminished by the soft-textured kernels. The Glu-D1 locus containing the subunits 1Dx2 + 1Dy12 has also been introgressed to create higher quality soft durum bread.
Publisher: Elsevier
Date: 2015
Publisher: CSIRO Publishing
Date: 2014
DOI: 10.1071/CPV65N1_FO
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