ORCID Profile
0000-0003-4816-2909
Current Organisation
Smithsonian Institution
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Publisher: Pensoft Publishers
Date: 16-09-2013
DOI: 10.3897/BDJ.1.E995
Publisher: GigaScience Press
Date: 30-06-2022
DOI: 10.46471/GIGABYTE.64
Abstract: Insect silk is a versatile biomaterial. Lepidoptera and Trichoptera display some of the most erse uses of silk, with varying strength, adhesive qualities, and elastic properties. Silk fibroin genes are long ( Kbp), with many repetitive motifs that make them challenging to sequence. Most research thus far has focused on conserved N- and C-terminal regions of fibroin genes because a full comparison of repetitive regions across taxa has not been possible. Using the PacBio Sequel II system and SMRT sequencing, we generated high fidelity (HiFi) long-read genomic and transcriptomic sequences for the Indianmeal moth (Plodia interpunctella) and genomic sequences for the caddisfly Eubasilissa regina. Both genomes were highly contiguous (N50 = 9.7 Mbp/32.4 Mbp, L50 = 13/11) and complete (BUSCO complete = 99.3%/95.2%), with complete and contiguous recovery of silk heavy fibroin gene sequences. We show that HiFi long-read sequencing is helpful for understanding genes with long, repetitive regions.
Publisher: Brill
Date: 2009
Abstract: Schildia Aldrich, 1923, a distinctive and rarely collected genus of Leptogastrinae (Diptera: Asilidae), is revised. Ten species are recognized, of which four are new to science. The nine extant species are Afrotropical, Neotropical and Oriental in distribution. The extant Neotropical species are Schildia alphus Martin, 1975, Schildia caliginosa sp.n. (Ecuador and Venezuela), Schildia fragilis (Carrera, 1944), Schildia guatemalae Martin, 1975, Schildia gracillima (Walker, 1855), Schildia jamaicensis Farr, 1963, and Schildia microthorax Aldrich, 1923. The only extant Afrotropical species, Schildia adina sp.n., is described from extant and subfossilized specimens (Malagasy copal) from south-western Madagascar. The extant Oriental species, Schildia malaya sp.n., is described from northern Malaysia. One extinct species, Schildia martini sp.n., is newly described from Dominican amber. Two new synonyms are proposed: Schildia ocellata Martin, 1975 is a junior synonym of Schildia gracillima and Schildia zonae Martin, 1975 is synonymized with Schildia fragilis. Redescriptions and descriptions of the genus and all extant and extinct species are provided. An identification key to the extant and extinct species is presented. Illustrations, photographs, and scanning electron micrographs are provided to support the descriptions and key. Distribution, biogeography, occurrence in bio ersity hotspots, seasonal incidence and biology are discussed.
Publisher: Wiley
Date: 11-11-2017
DOI: 10.1111/SYEN.12275
Publisher: Cold Spring Harbor Laboratory
Date: 03-06-2022
DOI: 10.1101/2022.06.01.494423
Abstract: Insect silk is an incredibly versatile biomaterial. Lepidoptera and their sister lineage, Trichoptera, display some of the most erse uses of silk with varying strength, adhesive qualities and elastic properties. It is well known that silk fibroin genes are long ( 20 kb) and have many repetitive motifs. These features make these genes challenging to sequence. Most research thus far has focused on conserved N- and C-terminal regions of fibroin genes because a full comparison of repetitive regions across taxa has not been possible. Using the PacBio Sequel II system and SMRT sequencing, we generated high fidelity (HiFi) long-read genomic and transcriptomic sequences for the Indianmeal moth ( Plodia interpunctella ) and genomic sequences for the caddisfly, Eubasilissa regina . Both genomes were highly contiguous (N50 = 9.7 Mbp/32.4 Mbp, L50 = 13/11) and complete (BUSCO Complete = 99.3%/95.2%), with complete and contiguous recovery of silk heavy fibroin gene sequences. This study demonstrates that HiFi long-read sequencing can significantly help our understanding of genes with highly contiguous, repetitive regions.
Location: United States of America
No related grants have been discovered for Torsten Dikow.