ORCID Profile
0000-0003-4441-4988
Current Organisations
Prince of Songkla University
,
Mahidol University Faculty of Medicine Siriraj Hospital
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Publisher: Springer Science and Business Media LLC
Date: 04-04-2023
DOI: 10.1038/S43856-023-00268-Y
Abstract: Pregnant women are at greater risk of adverse outcomes, including mortality, as well as obstetrical complications resulting from COVID-19. However, pregnancy-specific changes that underlie such worsened outcomes remain unclear. Plasma s les were collected from pregnant women and non-pregnant in iduals (male and female) with (n = 72 pregnant, 52 non-pregnant) and without (n = 29 pregnant, 41 non-pregnant) COVID-19. COVID-19 patients were grouped as asymptomatic, mild, moderate, severe, or critically ill according to NIH classifications. Proteomic profiling of 7,288 analytes corresponding to 6,596 unique protein targets was performed using the SOMAmer platform. Herein, we profile the plasma proteome of pregnant and non-pregnant COVID-19 patients and controls and show alterations that display a dose-response relationship with disease severity yet, such proteomic perturbations are d ened during pregnancy. In both pregnant and non-pregnant state, the proteome response induced by COVID-19 shows enrichment of mediators implicated in cytokine storm, endothelial dysfunction, and angiogenesis. Shared and pregnancy-specific proteomic changes are identified: pregnant women display a tailored response that may protect the conceptus from heightened inflammation, while non-pregnant in iduals display a stronger response to repel infection. Furthermore, the plasma proteome can accurately identify COVID-19 patients, even when asymptomatic or with mild symptoms. This study represents the most comprehensive characterization of the plasma proteome of pregnant and non-pregnant COVID-19 patients. Our findings emphasize the distinct immune modulation between the non-pregnant and pregnant states, providing insight into the pathogenesis of COVID-19 as well as a potential explanation for the more severe outcomes observed in pregnant women.
Publisher: Walter de Gruyter GmbH
Date: 21-06-2023
Abstract: To determine whether the maternal plasma concentrations of cytokines are higher in pregnant women with postpartum hemorrhage (PPH) compared to pregnant women without PPH. A retrospective case-control study included 36 women with PPH and 72 matched controls. Cases and controls were matched for gestational age at delivery, labor status, delivery route, parity, and year of s le collection. Maternal plasma s les were collected up to 3 days prior to delivery. Comparison of the plasma concentrations of 29 cytokines was performed by using linear mixed-effects models and included adjustment for covariates and multiple testing. A false discovery rate adjusted p-value .1 was used to infer significance. Random forest models with evaluation by leave-one-out and 9-fold cross-validation were used to assess the combined value of the proteins in predicting PPH. Concentrations of interleukin (IL)-16, IL-6, IL-12/IL-23p40, monocyte chemotactic protein 1 (MCP-1), and IL-1β were significantly higher in PPH than in the control group. This difference remained significant after adjustment for maternal age, clinical chorioamnionitis, and preecl sia. Multi-protein random forest proteomics models had moderate cross-validated accuracy for prediction of PPH [area under the ROC curve, 0.69 (0.58–0.81) by leave-one-out cross validation and 0.73 (0.65–0.81) by 9-fold cross-validation], and the inclusion of clinical and demographic information did not increase the prediction performance. Pregnant women with severe PPH had higher median maternal plasma concentrations of IL-16, IL-6, IL-12/IL-23p40, MCP-1, and IL-1β than patients without PPH. These cytokines could serve as biomarkers or their pathways may be therapeutic targets.
Publisher: Walter de Gruyter GmbH
Date: 27-12-2023
Abstract: An abnormal angiogenic profile is present in about one-half of women with preecl sia at term. Few studies examined the roles of angiogenic biomarkers in ecl sia. The aims of this study were to determine (1) whether the degree of an anti-angiogenic state, reflected by a low placental growth factor (PlGF) to soluble fms-like tyrosine kinase-1 (sFlt-1) ratio, in women with ecl sia differed from that of women with severe preecl sia and (2) the prevalence of women who had an abnormal angiogenic profile at the diagnoses of preterm and term ecl sia. A cross-sectional study was conducted to include women in the following groups: (1) uncomplicated pregnancy (n=40) (2) severe preecl sia (n=50) and (3) ecl sia (n=35). Maternal serum concentrations of PlGF and sFlt-1 were determined by immunoassays. Women with preterm, but not term, ecl sia had a more severe anti-angiogenic state than those with severe preecl sia ( lower PlGF and PlGF/sFlt-1 ratio, each p .05). However, the difference diminished in magnitude with increasing gestational age (interaction, p=0.005). An abnormal angiogenic profile was present in 95% (19/20) of women with preterm ecl sia but in only 67% (10/15) of women with ecl sia at term. Angiogenic biomarkers can be used for risk assessment of preterm ecl sia. By contrast, a normal profile of angiogenic biomarkers cannot reliably exclude patients at risk for ecl sia at term. This observation has major clinical implications given that angiogenic biomarkers are frequently used in the triage area as a test to rule out preecl sia.
Publisher: Research Square Platform LLC
Date: 14-12-2022
DOI: 10.21203/RS.3.RS-2359402/V1
Abstract: Spontaneous preterm birth (sPTB) can occur when vaginal bacteria gain access to the amniotic cavity. Thus, the predictive value of the vaginal microbiota for sPTB has been investigated, yet results have been inconclusive. Here, we report the largest study of the vaginal microbiota using longitudinal s ling of 257 cases and 514 controls (2,976 s les). Associations between the microbiota and sPTB were limited to cases of early (delivery weeks) preterm prelabor rupture of membranes (PPROM). Starting with early gestation, microbial ersity was higher in early PPROM cases than controls, as were the relative abundances of Anaerococcus, Mobiluncus, Prevotella, and Sneathia. Random forest models based on data collected before 28 weeks predicted early PPROM [AUC=0.62(0.51−0.73)], while data collected by 24 weeks predicted PPROM with delivery weeks [AUC=0.68(0.53-0.84)]. Therefore, monitoring of the vaginal microbiota profile may have clinical utility to identify a subset of women who will deliver a preterm neonate.
Publisher: Springer Science and Business Media LLC
Date: 11-07-2022
DOI: 10.1038/S41598-022-15392-3
Abstract: Preterm birth, the leading cause of perinatal morbidity and mortality, is associated with increased risk of short- and long-term adverse outcomes. For women identified as at risk for preterm birth attributable to a sonographic short cervix, the determination of imminent delivery is crucial for patient management. The current study aimed to identify amniotic fluid (AF) proteins that could predict imminent delivery in asymptomatic patients with a short cervix. This retrospective cohort study included women enrolled between May 2002 and September 2015 who were diagnosed with a sonographic short cervix ( 25 mm) at 16–32 weeks of gestation. Amniocenteses were performed to exclude intra-amniotic infection none of the women included had clinical signs of infection or labor at the time of amniocentesis. An aptamer-based multiplex platform was used to profile 1310 AF proteins, and the differential protein abundance between women who delivered within two weeks from amniocentesis, and those who did not, was determined. The analysis included adjustment for quantitative cervical length and control of the false-positive rate at 10%. The area under the receiver operating characteristic curve was calculated to determine whether protein abundance in combination with cervical length improved the prediction of imminent preterm delivery as compared to cervical length alone. Of the 1,310 proteins profiled in AF, 17 were differentially abundant in women destined to deliver within two weeks of amniocentesis independently of the cervical length (adjusted p-value 0.10). The decreased abundance of SNAP25 and the increased abundance of GPI, PTPN11, OLR1, ENO1, GAPDH, CHI3L1, RETN, CSF3, LCN2, CXCL1, CXCL8, PGLYRP1, LDHB, IL6, MMP8, and PRTN3 were associated with an increased risk of imminent delivery (odds ratio 1.5 for each). The sensitivity at a 10% false-positive rate for the prediction of imminent delivery by a quantitative cervical length alone was 38%, yet it increased to 79% when combined with the abundance of four AF proteins (CXCL8, SNAP25, PTPN11, and MMP8). Neutrophil-mediated immunity, neutrophil activation, granulocyte activation, myeloid leukocyte activation, and myeloid leukocyte-mediated immunity were biological processes impacted by protein dysregulation in women destined to deliver within two weeks of diagnosis. The combination of AF protein abundance and quantitative cervical length improves prediction of the timing of delivery compared to cervical length alone, among women with a sonographic short cervix.
Location: Thailand
No related grants have been discovered for Manaphat Suksai.