ORCID Profile
0000-0002-0956-1125
Current Organisation
Bogor Agricultural University
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: UNS Solo
Date: 19-12-2022
Abstract: Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2022. Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics. Bio ersitas 23: 6448-6455. Anaerobic fungi are essential microbes in the degradation process of feed fiber in the rumen with the ability to produce fibrolytic enzymes and rhizoids that can penetrate feed particles. The activity of cellulase enzymes in fungi is influenced by several factors such as the type of feed and livestock used as a source of isolate. Therefore, this research aimed to analyze the types of anaerobic fungi isolated from buffalo based on their DNA nitrogenous bases composition and also to evaluate the growth rate, cellulase enzyme activity, and fermentation product concentration of anaerobic fungi. The growth rate of fungi was examined by measuring the biomass formed, while cellulase enzyme activity was carried out using CMC, Avicel, and Filter Paper as substrates. The fungal fermentation products were analyzed using the HPLC and GC methods. The results showed that the fungi isolated from buffalo rumen were closely related to anaerobic fungi type Piromyces sp., Caecomyces sp., and Neocallimastix frontalis, with different growth rate, cellulase enzyme activity, and the fermentation products concentration in each type of anaerobic fungi. Therefore, it can be concluded that anaerobic fungi isolated from buffalo rumen can degrade cellulose. It was also discovered that fungi Neocallimastix frontalis had a higher growth rate, cellulase enzyme activity (CMCase, avicelase, and FPase), and fermentation products than Piromyces sp. and Caecomyces sp.
Publisher: UNS Solo
Date: 06-01-2022
Abstract: Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2021. The enrichment process and morphological identification of anaerobic fungi isolated from buffalo rumen. Bio ersitas 23: 469-477. Anaerobic fungi are one of the microbes that have an important role in rumen fiber degradation because they can produce cellulase enzymes and penetrate feed particles. Nevertheless, few studies were performed to test the potential of anaerobic fungi in Indonesia. Therefore, the present study was carried out to evaluate the impact of the enrichment process on pH value, the zoospores population, NH3 (ammonia) concentration, and VFA (Volatile Fatty Acid) proportion. In addition, this research was also performed to isolate anaerobic fungi from buffalo rumen and identify their morphological characteristics. The enrichment stage of anaerobic fungi was carried out using the Hungate method. Results showed that the population of fungi zoospores, pH value, ammonia concentration, the proportion of acetate, and total VFA were significantly affected by the incubation time (P .01). In addition, Caecomyces, Neocallimastix, and Piromyces were rumen anaerobic fungi isolated from buffalo rumen with different morphological characteristics. It can be concluded that the incubation time increased the zoospore population, the concentration of NH3, acetate proportion, and total VFA but decreased media's pH value.
No related grants have been discovered for Anja Meryandini.