ORCID Profile
0000-0001-5039-8764
Current Organisation
Cell Therapy Center, The University of Jordan
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Publisher: Springer Science and Business Media LLC
Date: 06-09-2023
Publisher: Walter de Gruyter GmbH
Date: 29-03-2021
Abstract: Column chromatography (CC) analysis of methanol and butanol extracts of the aerial parts of Calortopis procera as well as the methanol extract of its latex, led to the isolation of 8 cardenolides, of which the structures were elucidated by NMR and HRESIMS spectroscopy. They also revealed several triterpenes and flavonoid glycoside. Based on the antiproliferative activity reported for cardenolides, the activity of calotropin and calotoxin was tested against two common cancer cell lines, human triple-negative breast cancer cell line (MDA-MB-231) and human lung adenocarcinoma cell line (A549). The high toxicity of the latex also encouraged performing the same test on the same cancer cell lines. The anti-proliferative activity of calotropin and calotoxin was compared to the methanol extract and the wax of the latex. The results showed that calotropin and calotoxin have significant cytotoxicity against MDA-MB-231 and A549 cell lines ranging from 0.046 to 0.072 μM compared to the methanol extract and the wax of its latex ranging from 0.47 to 58.41 μM. Moreover, the results showed lower toxicity of all treatments to the human skin fibroblasts compared to the toxicity to both MDA-MB-231 and A549 cancer cells lines except the higher toxicity of Methanolic extracts of C. procera latex to the MDA-MB-231 cells. In conclusion, C. procera is a medicinal plant with a wide spectrum of cardinolides including calotropin and calotoxin, which are promising agents for targeted cancer phytotherapy.
Publisher: BENTHAM SCIENCE PUBLISHERS
Date: 13-06-2022
DOI: 10.2174/9789815051278122010008
Abstract: Theranostic nanomaterials hold the potential to revolutionize future disease management. Recent progress in nanomaterials technology and aptamer-base- -targeting molecules have promoted efficient theranostics models. Aptamers are unique three-dimensional structures consisting of oligonucleotide (25-80 nt) polymers. They are comparable to monoclonal antibodies in their receptor-driven binding efficacy toward specific target receptors and binding ability to specific target molecules with high affinity and specificity. Aptamers have several other advantages, including prolonged shelf life, little or no variation from batch to batch, and ease of chemical modifications for enhanced stability and targeting capacity. Owing to the advantages mentioned above, aptamers are attracting great attention in erse applications ranging from therapy, drug delivery, diagnosis, and functional genomics as well as biosensing. Herein, the aim is to give an overview of aptamers, highlight the opportunities of their application as means of effective therapeutic tools as well as functionalize them as potential diagnostic probes. Furthermore, the erse modifications of aptamers for theranostic purposes, including therapeutic agents and targeted delivery nanomaterials, are comprehensively summarized.
Publisher: MDPI AG
Date: 18-12-2019
DOI: 10.3390/MOLECULES25010003
Abstract: Soon after they were first described in 1990, aptamers were largely recognized as a new class of biological ligands that can rival antibodies in various analytical, diagnostic, and therapeutic applications. Aptamers are short single-stranded RNA or DNA oligonucleotides capable of folding into complex 3D structures, enabling them to bind to a large variety of targets ranging from small ions to an entire organism. Their high binding specificity and affinity make them comparable to antibodies, but they are superior regarding a longer shelf life, simple production and chemical modification, in addition to low toxicity and immunogenicity. In the past three decades, aptamers have been used in a plethora of therapeutics and drug delivery systems that involve innovative delivery mechanisms and carrying various types of drug cargos. However, the successful translation of aptamer research from bench to bedside has been challenged by several limitations that slow down the realization of promising aptamer applications as therapeutics at the clinical level. The main limitations include the susceptibility to degradation by nucleases, fast renal clearance, low thermal stability, and the limited functional group ersity. The solution to overcome such limitations lies in the chemistry of aptamers. The current review will focus on the recent arts of aptamer chemistry that have been evolved to refine the pharmacological properties of aptamers. Moreover, this review will analyze the advantages and disadvantages of such chemical modifications and how they impact the pharmacological properties of aptamers. Finally, this review will summarize the conjugation strategies of aptamers to nanocarriers for developing targeted drug delivery systems.
No related grants have been discovered for Mohammad Ismail.