ORCID Profile
0000-0001-5302-571X
Current Organisations
Matamata Veterinary Services
,
Texas A&M University
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Publisher: Elsevier BV
Date: 07-2017
Publisher: FapUNIFESP (SciELO)
Date: 02-2013
DOI: 10.1590/S0103-84782013005000009
Abstract: Pulmonary inflammatory diseases have been observed in equine since their domestication. Non-infectious disease processes of the lower airways are amongst the most important diseases of the equine respiratory tract. This study aims to determine the cytology profile and effect of age on the evaluation of equine bronchoalveolar lavage (BAL) obtained from healthy animals and from horses with asymptomatic inflammatory airway disease (IAD). Thirty two horses, including 20 males and 12 females, without clinical signs of pulmonary disease and with age varying from 4 to 21 years, were assigned to two groups: 15 animals in the control group and 17 in the asymptomatic group. Groups were ided according to physical examination and mucous accumulation observed during tracheal endoscopy. The BAL results from healthy and asymptomatic horses were, respectively: 56.9% and 48.0% macrophages, 37.2% and 40.0% lymphocytes, 3.3% and 7.1% neutrophils, 1.7% and 1.4% mast cells, 0.3% and 0.7% eosinophils 0,5% and 0.4% epithelial cells. When horses in the two groups were analyzed according to their age range, no differences were found. In conclusion, IAD in the asymptomatic group was significantly different from the control group. The asymptomatic horses presented a discrete infiltrate of neutrophils, which does not influence cytological evaluation of BAL.
Publisher: Elsevier BV
Date: 11-2020
Publisher: FapUNIFESP (SciELO)
Date: 2023
DOI: 10.1590/1809-6891V24E-74190P
Abstract: Resumo O impacto do exercício de alta velocidade no sistema musculoesquelético de cavalos de corrida jovens tem sido lamente discutido devido a preocupações com a saúde e o bem-estar animal. Este estudo investigou a correlação entre idade, grau de ossificação da epífise radial distal, sexo e longevidade da carreira de cavalos Puro Sangue Inglês de corrida no Brasil de 2012 a 2015. Realizamos uma avaliação retrospectiva de 286 radiografias dorsopalmar da região radiocarpica esquerda de cavalos jovens e seu desempenho de corrida. O fechamento epifisário radial distal foi classificado em três graus decrescentes: A, B ou C. Os dados de desempenho incluíram o número de corridas disputadas, duração da carreira atlética e o número de corridas por mês. As variáveis foram submetidas à análise de regressão. No momento do exame radiográfico, os cavalos machos eram significativamente mais velhos que as fêmeas, e os cavalos com graus de fechamento epifisário diferiram com a idade. A idade na primeira corrida foi de 33,08±3,81 meses, a média de corridas disputadas foi de 18,32±15,14 corridas, a duração da carreira atlética foi de 20,37±13,82 meses e o número de corridas realizadas por mês foi de 0,93±0,46 corridas. A idade influenciou (P ,001) o fechamento da epífise radial distal em cavalos de corrida, mas o sexo não (P=0,218 para machos e P=0,275 para fêmeas). Uma associação inversa foi observada entre a idade na primeira corrida, o número de corridas disputadas por mês e a duração da carreira atlética. A frequência de corrida e a idade na primeira corrida influenciaram a duração da carreira atlética.
Publisher: Brill
Date: 06-06-2018
DOI: 10.3920/CEP170032
Abstract: Exercise induced pulmonary haemorrhage (EIPH) affecting racehorses continues to raise questions regarding animal welfare and to-date no effective treatment has been identified. The mode of action of furosemide on EIPH, the only medication for the condition accepted in some racing jurisdictions, has not been completely elucidated. This research investigated the interaction between furosemide on angiotensin converting enzyme (ACE) as a potential pathway for future investigation of EIPH treatment options in a prospective case-control analytical study. ACE is a potent vasoconstrictor and substances reducing its activity could potentially contribute to decreasing blood pressure and EIPH. Horses racing on 8 official race days at Gávea Racetrack, Brazil had respiratory endoscopy data and blood s les collected after the race and were grouped into furosemide medicated and non-furosemide medicated horses. ACE measurement was conducted using fluorescence in a previously validated method. Environmental, race and haematological data were also recorded. A multiple regression model was used to analyse the data collected, with further analysis including Fisher’s exact test and Pearson’s chi-squared test with Yates’ continuity correction a Welch two s le t-test and a simple linear regression model. 73 horses were included in the study. ACE activity between horses not medicated and medicated with furosemide was significantly different. Pre-race furosemide significantly influenced ACE activity post-race, while distance raced, temperature, humidity, and haematocrit did not. Horses medicated with pre-race furosemide still demonstrated some degree of bleeding after the race and were at higher risk of presenting EIPH than non-medicated horses. Horses medicated with furosemide have lower circulating ACE activity which might indicate a protective effect of furosemide. Furosemide might reduce EIPH severity after a single bout of exercise, but it does not abolish or reduce its occurrence.
Publisher: Wiley
Date: 11-2010
DOI: 10.1111/J.2042-3306.2010.00171.X
Abstract: Investigate angiotensin converting enzyme (ACE) activity in equine plasma as a predictor of performance in endurance competitions and the effect of endurance exercise on ACE activity. Precompetition values of ACE activity in equine blood are correlated with performance results and with heart rates pre- and post competition used as indicators of fitness. Endurance exercise increases ACE activity. Nineteen horses participating in an 80 km endurance competition had venous blood s les collected before and after the ride. ACE activity and total protein were measured in the blood s les and heart rates and finishing positions were recorded. Statistical analysis included paired t tests and Spearman's rank correlation coefficient. Of the 19 horses enlisted, only 16 horses completed the ride. Of these 16, another 2 were disqualified at the last veterinary check. When the 16 horses were considered, precompetition heart rate, but not ACE, was correlated with finishing position. When only the 14 horses that were classified were considered, the association disappeared. ACE activity was similar before and after competition. Precompetition ACE activity in endurance horses competing in an 80 km event was not associated with either finishing position or heart rates before or after competition, indicating that the enzyme is not a good predictor of performance in this form of equestrian competition. Endurance competition did not significantly alter ACE activity in this group of horses.
Publisher: MDPI AG
Date: 09-10-2012
DOI: 10.3390/BIOS2040396
Publisher: Wiley
Date: 08-2006
DOI: 10.1111/J.2042-3306.2006.TB05592.X
Abstract: To our knowledge no studies evaluating risk factors to EIPH have been undertaken with such a population of horses in Brazil, which is one of the few countries in the racing community to accept the use of frusemide (FUR) as a prerace medication for bleeders. We also compared national results with those already in the literature. To verify the influence of variables such as distance of the race, racetrack surface, going and season of the year in the recurrence and severity of EIPH and its effect on the finishing position of flat racing Thoroughbreds in Brazil. 2118 post race respiratory endoscopies were recorded, in a total of 1003 in idual horses. All horses in the study were certified bleeders (deemed positive by registered veterinarians following one or more endoscopies) and were administered FUR prior to each race. Data was analysed using both ordinary logistic regression and multiple logistic regression. The results suggest that race distance, season of the year and racetrack surface affect the recurrence of EIPH. Also, despite the use of FUR, the majority (62%) of horses continued to display some degree of haemorrhage. There was a tendency for horses that remained positive despite the use of FUR to finish unplaced in a race. Horses which had a significant reduction in bleeding and became HPIE negative after the use of FUR, tended to finish in top positions. It was not possible to determine a pattern of severity of EIPH in horses with more than one endoscopy. Further studies are required to improve our understanding of this syndrome and its correlations to performance in the racing Thoroughbred, together with the role of FUR and its possible enhancing performance effects and efficacy in consistently diminishing EIPH. This study enabled a better understanding of the use of FUR in Brazilian Thoroughbred racehorses and showed there was a tendency towards improvement in performance after administrion of FUR in bleeders. The fact that a large number of horses remained positive for EIPH despite the use of FUR should encourage the scientific community to search for better ways of reducing, or preventing, this syndrome.
Publisher: FapUNIFESP (SciELO)
Date: 2023
DOI: 10.1590/1809-6891V24E-74190E
Abstract: Abstract The impact of high-speed exercise on the musculoskeletal system of young racehorses has been extensively discussed because of concerns regarding animal health and welfare. This study investigated the correlation between age, degree of ossification of the distal radial epiphysis, sex, and career longevity of Thoroughbred racehorses in Brazil from 2012 to 2015. We performed a retrospective evaluation of 286 dorsopalmar radiographs of the left radiocarpal region of young horses and their racing performance. Distal radial epiphyseal closure was classified into three degrees: A, B, or C. Performance data included the number of races raced, athletic career length, and the number of races per month. The variables were subjected to regression analysis. At the time of radiographic examination, male horses were significantly older than females, and horses with epiphyseal closure degrees differed with age. Age at first race was 33.08±3.81 months, the average of races raced was 18.32±15.14 races, athletic career duration was 20.37±13.82 months, and the number of races raced per month was 0.93±0.46 races. Age influenced (P .001) the distal radial epiphyseal closure on racehorses, but sex did not (P=0.218 for males and P=0.275 for females). An inverse association was observed between age at the first race, the number of races raced per month, and athletic career duration. The frequency of race and the age at the first race influenced athletic career duration.
Publisher: Springer Science and Business Media LLC
Date: 04-02-2020
DOI: 10.1186/S13104-020-4930-8
Abstract: R-spondin 2 (RSPO2) is required for lung morphogenesis, activates Wnt signaling, and is upregulated in idiopathic lung fibrosis. Our objective was to investigate whether RSPO2 is similarly important in homeostasis of the adult lung. While investigating the characteristics of bronchoalveolar lavage in RSPO2-deficient (RSPO2 −/− ) mice, we observed unexpected changes in neutrophil homeostasis and vascular permeability when compared to control (RSPO2 +/+ ) mice at baseline. Here we quantify these observations to explore how tonic RSPO2 expression impacts lung homeostasis. Quantitative PCR (qPCR) analysis demonstrated significantly elevated myeloperoxidase (MPO) expression in bronchoalveolar lavage fluid (BALF) cells from RSPO2 −/− mice. Likewise, immunocytochemical (ICC) analysis demonstrated significantly more MPO+ cells in BALF from RSPO2 −/− mice compared to controls, confirming the increase of infiltrated neutrophils. We then assessed lung permeability/barrier disruption via Fluorescein Isothiocyanate (FITC)-dextran instillation and found a significantly higher dextran concentration in the plasma of RSPO2 −/− mice compared to identically treated RSPO2 +/+ mice. These data demonstrate that RSPO2 may be crucial for blood-gas barrier integrity and can limit neutrophil migration from circulation into alveolar spaces associated with increased lung permeability and/or barrier disruption. This study indicates that additional research is needed to evaluate RSPO2 in scenarios characterized by pulmonary edema or neutrophilia.
Publisher: FapUNIFESP (SciELO)
Date: 06-2019
Abstract: ABSTRACT Alveolar macrophages (AMs) are an essential part of defense mechanisms within the lungs and their phagocytic activity is important for organ homeostasis. The phagocytic ability of AMs obtained from bronchoalveolar lavage from 17 mature mixed-breed pleasure horses (8 healthy and 9 diagnosed with mild equine asthma) was studied through assays with Leishmania (Viannia) braziliensis promastigotes, which enabled the calculation of a phagocytic index (PI) and a survival index (SI). Results indicate that phagocytic activity of AMs in asthma affected horses is similar to healthy horses, while leishmanicidal activity is significantly increased in horses with asthma.
Publisher: Wiley
Date: 18-01-2021
DOI: 10.1111/EVE.13418
Abstract: The objectives were: 1. to investigate the occurrence of equine asthma syndrome (EAS) in a group of C olina and Mangalarga Marchador horses through post‐exercise respiratory endoscopy and cytology 2. compare the efficiency of different diagnostic criteria and 3. compare the incidence of EAS by breed, age group and sex. A standardised exercise test was conducted in the field, followed by respiratory endoscopy and bronchoalveolar lavage and fluid collection 30 min post‐exercise. Clinical evaluation pre‐ and post‐exercise, respiratory endoscopy post‐exercise and bronchoalveolar lavage, also post‐exercise, were conducted in 30 horses (15 C olina and 15 Mangalarga Marchador) aged 3–18 years, without a history of respiratory illness and without clinical evidence of active respiratory disease. All horses were in active training and were considered fit to participate in equestrian competition characteristic of these breeds. Post‐collection, the horses were sub ided into healthy or EAS‐positive, depending on several diagnostic criteria. Statistical comparisons between breeds, age brackets (up to 7 years of age and above 7 years of age) and sex were conducted using appropriate statistical tests. According to bronchoalveolar lavage fluid (BALF) cytology, 56.7% of the horses showed at least one inclusion criteria that contributed to classification as EAS‐positive. Of those, only one was classified as moderate EAS, while the others were classified as mild. Total cell count in the BALF above 530 cells/µL was the single most common diagnostic criteria (100%), followed by increased tracheal mucus (53%) at endoscopy, eosinophilia (47%) and neutrophilia (35%) in BALF. Significant differences were found between macrophage and lymphocyte numbers according to breed, but no other differences between breeds, age brackets, sex or status (healthy vs. EAS‐positive). A high occurrence of EAS was found in competition‐ready Mangalarga and C olina pacer horses in this study.
Publisher: Editora Cubo
Date: 2005
Publisher: FapUNIFESP (SciELO)
Date: 2017
DOI: 10.1590/0103-8478CR20160861
Abstract: ABSTRACT: Athletic horses need to maintain healthy airways for optimal performance. This study investigated the presence of respiratory problems in apparently healthy Thoroughbred racehorses in training. According to the Revised Consensus Statement on Inflammatory Airway Diseases of Horses (2016), determining the prevalence of respiratory disorders in different equine populations is fundamental for understanding these diseases. In total, 72 clinically sound Thoroughbred racehorses, in training at the Brazilian Jockey Club (JCB), were initially examined using interpleural pressure measurement by ventigraphy and respiratory endoscopy. When secretions were present in the airways, transendoscopic tracheal aspiration was performed, and the s le was sent for cytology. The main findings included a combination of bronchospasm and tracheal secretions with 61% of the cytology slides showing neutrophil counts ≥20%. Overall, a significant number of the horses displayed signs suggestive of inflammatory airway disease (mild equine asthma), including 47% with increased DPplmax, 11% with tracheal mucus accumulation [mucus score (MS) ≥2] and 18% with carina edema. This was more pronounced in 2-year-old horses within the population studied. These findings are consistent with the literature and reinforce the importance of routine respiratory examination of athletic horses. There is a high incidence of subclinical respiratory disorders in Thoroughbred racehorses in training at the JCB and a significant association between tracheal MS≥2, carina edema, and elevated DPplmax.
Publisher: Universidade Severino Sombra
Date: 04-06-2018
Abstract: Racehorses are commonly affected by a condition known as Exercise Induced Pulmonary Haemorrhage (EIPH) which consists of rupture of alveolar membranes and extravasation of blood into the airways. EIPH is considered to negatively affect racehorse performance and there is no current treatment. In order to reduce the amount of bleeding the diuretic furosemide has been used prior to exercise and competition in some racing jurisdictions. Efficacy of furosemide in reducing EIPH is documented in this research through endoscopic observation of the airways of horses positive for EIPH in two consecutive races.
Publisher: Elsevier BV
Date: 08-2017
Publisher: FapUNIFESP (SciELO)
Date: 08-2019
DOI: 10.1590/1678-5150-PVB-5883
Abstract: ABSTRACT: This study compared two protocols for preparation of platelet rich plasma (PRP) and evaluated the association between manual and automated methods for platelet count using a prospective study design. Eight clinically healthy Quarter Horses had venous blood s les collected at rest. After collection, blood s les were centrifuged twice, using two different protocols including a period of s le resting, either at the start or at the end of the protocol. Platelet counting at the start of the protocol, during, and after obtaining PRP was conducted manually or with an automated counter, followed by comparison of the two methods. In order to investigate platelet degranulation during the protocol, vascular endothelial growth factor (VEGF) was measured at each preparation stage. The protocol with s le resting before centrifugation yielded a more concentrated PRP, and the study verified that both manual and automated methods are comparable and can be used interchangeably for platelet counting. VEGF concentration did not differ significantly between protocols, or among protocol stages. The results indicate that choice of protocol for PRP preparation will affect the quantity of platelets in the final product, although platelet degranulation was not observed as evidenced by the stable VEGF concentrations measured. A larger yield of non-degranulated platelets in PRP is desirable since more α-granules will be present, therefore Protocol II is recommended. Both manual and automated counts reliably allow clinicians to obtain platelet counts and the choice of utilizing a manual or automated method is unlikely to interfere with evaluation of the final PRP product.
Publisher: Wiley
Date: 15-03-2011
DOI: 10.1111/J.2042-3306.2010.00320.X
Abstract: Studies in man have shown a correlation between Angiotensin I-converting enzyme (ACE) genetic polymorphisms, ACE activity in the blood and superior athletic performance in sports requiring endurance. It has been hypothesised that the same correlation occurs in horses. There is no information in the literature concerning the effects of training on ACE activity in equine plasma. Exercise training influences the activity of circulating ACE and the response observed is dependent on the exercise protocol. Thirteen horses of mixed breeds were randomly allocated 2 different training protocols to be carried out for a period of 15 weeks. Blood s les were collected from each horse before the beginning of training to determine baseline values. Subsequent s ling took place every 15 days throughout the training phase and for 8 weeks of paddock rest. Angiotensin I-converting enzyme activity was determined by automated spectrophotometry. Training for 15 weeks significantly increased plasma ACE activity, irrespective of training protocol. Differences observed in ACE activity pattern between the 2 training protocols were not statistically significant. Increase in ACE activity peaked with maximum workload. As soon as training was interrupted, ACE levels significantly decreased. Exercise training affects levels of ACE activity in equine plasma. The mechanism for this is not yet elucidated, but cardiovascular adaptation to exercise and blood pressure changes might be involved in this regulation. Exercise training produced a gradual increase in enzymatic activity and might warrant the use of ACE as a tool for fitness monitoring. Angiotensin I-converting enzyme enzymatic activity in the plasma might be directly correlated to a change in genetic expression and that variability must be taken into account when evaluating results from horses undergoing a physical training programme.
Publisher: Universidade Federal do Rio Grande do Sul
Date: 2021
DOI: 10.22456/1679-9216.107923
Abstract: Background: Summer Pasture Associated Obstructive Pulmonary Disease (SPAOPD), or Equine Pasture Asthma (EPA), as termed by Ferrari et al. [17], has been described as an environmentally-induced respiratory disease that occurs during the warmer and more humid months, leading to reversible airway obstruction, persistent and non-specific airway hyper-responsiveness, and chronic neutrophilic airway inflammation. Exacerbation of clinical signs vary according to warm seasons, and range from mild to severe episodes of wheezing, coughing, and laboured breathing in a chronic state that is debilitating for the equine [4]. This report describes two cases of Equine Pasture Asthma that show clinical and environmental similarities with Summer Pasture Associated Obstructive Pulmonary Disease.Case: The patients were crossbreed geldings that have never been stabled and were used for cattle management in a farm in southeastern Brazil. They presented poor performance and a persistent cough for over 3 years. Initially, the respiratory signs were only observed after exercise but, over the years, it gradually progressed to being observed when the horses were at rest. Both animals had a history of regular deworming and were previously treated by other veterinarians with antibiotics, clenbuterol, and mucokinetics. Little improvement was noticed by the owner and the signs returned over time as treatment was often discontinued. Clinical findings were compatible with the grade 3 mucus classification of Gerber et al. [18] as well as with score 2 for Severe Asthma of Davis and Sheats [13]. BALF cytology was done following the technique described by Couetil et al. [10]. Animal 1 presented slides with free yeast macrophages and mucus with Curschmann’s spiral and counting of 29,7% of neutrophils (NE), 43,7% of lymphocytes (LP), 25,3% of macrophages (MC) and 1,3% of eosinophils (EO). Animal 2 presented slides with phagocytized yeast, mucus and counting of 27% of NE, 38,5% of LP, 33% of MC and 1,5% of EO.Discussion: Diagnostic findings fit the clinical score 2 (from 0 to 3) for Severe Asthma [13], where the animal presented frequent cough with periods of no coughing, nostrils flares in inspiration and exhalation, obvious abdominal flattening and “heave line”, pulmonary auscultation with crackles, and scarce mucous nasal discharge. The cytological findings of our reported cases also falls within the Severe Asthma classification [13], when the specific counting of 300 cells is equivalent to ≥20% of neutrophils on BALF analysis and the animal present increase in respiratory rate/effort at rest. Similar counts were found by Costa et al. [9] counting 200 cells, Rossi et al. [28] also counting 300 cells, and Couetil and Thompson [11] counting 5 fields (of at least 100 cells) on a cytocentrifuge smear. The present report took place in a region of the Rio de Janeiro State, southeastern Brazil, which shows predisposing climatic characteristics similar to those described in previous SPAOPD reports. Yet, this very climate remains somewhat constant throughout the year, as seasonality in the Rio de Janeiro State is not as marked as in the Northern Hemisphere. Given this contrasting aspect, we believe that the term Equine Pasture Asthma, instead of SPAOPD, is more appropriate to describe the cases presented here. Also, to the best of the authors’ knowledge, this is the first documented description in Brazil of Equine Pasture Asthma in animals that were never stabled or fed with hay. This documented evidence of a chronic respiratory condition consistent with Equine Pasture Asthma but little related to seasonal changes presents as a warning to other possible cases that might be unnoticed in equine herds in Brazil and in similar climates.
Publisher: FapUNIFESP (SciELO)
Date: 05-2021
Abstract: ABSTRACT The high prevalence of exercise-induced pulmonary hemorrhage (EIPH) in athletic horses constitutes to be a challenge to the racing industry and a source of major concern to animal welfare. Both experimental and clinical evidence indicate that the use of autologous platelet-rich plasma (PRP) is a promising effector of repair in a variety of pulmonary conditions. The present study evaluated the effect of intrabronchial instillation of PRP on EIPH endoscopic scores from 37 Thoroughbred racehorses. Inclusion criteria were for animals to be EIPH-positive in, at least, two consecutive post-exercise endoscopic exams and to receive 250mg of furosemide IV four hours before racing. Animals were randomly assigned into 3 groups: placebo, control, and PRP instillation. All 37 Thoroughbred racehorses included had EIPH endoscopic scores pre- and post- treatment compared by statistical analysis. The bleeding score from the group receiving PRP was significantly lower than in the control and placebo groups. No adverse effects were observed in any animal during or after the experiment. It was possible to conclude that the intrabronchial instillation of autologous PRP was effective in reducing EIPH scores in racehorses receiving furosemide and that this bioproduct can be considered as a promising coadjuvant in controlling EIPH in athletic horses.
Publisher: American Association for the Advancement of Science (AAAS)
Date: 27-11-2020
Abstract: Repair of blood vessels after viral lung injury is achieved by resident endothelial cells and requires COUP-TF2.
Publisher: The Korean Society of Veterinary Science
Date: 2011
Publisher: Wiley
Date: 05-09-2012
DOI: 10.1111/J.2042-3306.2011.00461.X
Abstract: Angiotensin I-converting enzyme (ACE) level measurement in blood s les is an important tool in human medicine for the detection, treatment and control of diseases such as sarcoidosis and hypertension. Recently ACE has been advocated as being correlated to athletic aptitude in human athletes and a genetic polymorphism has been shown to be responsible for the enzymatic levels in the circulation. The objective of this research was to evaluate the effects of acute exercise in horses in order to increase the understanding of a possible correlation between ACE levels in plasma and performance in equine athletes. A standardised exercise test (SET) to fatigue was conducted on 8 horses and repeated venous blood collections carried out for ACE activity measurements before, during and after the SET. Our results show an increase in ACE activity up to fatigue and a return to baseline values at 30 min post exercise.
Publisher: Walter de Gruyter GmbH
Date: 12-2018
Abstract: Platelet rich plasma (PRP) is an autologous biological product harvested by consecutive centrifugations of whole blood and separation of plasma in a stepwise protocol. PRP has been successfully used to stimulate healing in orthopedic and dermatological conditions, both in humans and animals. The principle is the fact that α- granules inside platelets contain a high concentration of growth factors, that once released can interfere with cellular communication and speed up healing. Standardization of PRP requires establishing a gold standard for the preparation and evaluation of the product, especially considering that platelet concentration and, therefore, growth factor concentration, might vary due to a number of variables. Factors such as age, gender, race or breed, and immune status of the patient might interfere with PRP quality and with treatment results, although little is known about such interferences. This research investigated the effect of breed/species and gender in platelet concentration in autologous PRP from horses and mules. The results demonstrate that Quarter Horses provided PRP with the greatest amount of platelets, although mules had a higher concentration percentages in relation to the initial platelet counts.
Publisher: Universidade Severino Sombra
Date: 15-06-2019
Abstract: O Reiki é uma terapia energética antiga, originada no século dezoito no Japão. Apesar de relatos não documentados do alívio de sintomas e melhoria na cicatrização de pacientes humanos e animais tratados com o Reiki, há pouca evidência científica que comprove sua eficácia, e menos ainda em relação ao seu modo de ação. Este trabalho investigou as alterações de temperatura de superfície antes, durante e após sessões de Reiki em 6 cavalos, com o auxílio de um termógrafo FLIR. Variações in iduais, associadas ao ponto de mensuração e ao tempo em relação ao tratamento com Reiki foram identificadas. Este estudo preliminar possibilitou a validação da metodologia a ser utilizada em estudos de maior escala, além de auxiliar na identificação de possíveis fontes de variação na temperatura de superfície em equinos tratados com Reiki. Mudanças de temperatura de superfície mais evidentes possivelmente seriam detectadas em animais doentes, em oposição a animais saudáveis. Estudos com maior número de animais e com a inclusão de medidas de temperatura corporal interna e perfil hematológico são necessários para esclarecer os efeitos mensuráveis da terapia Reiki em cavalos.
No related grants have been discovered for Maria Fernanda De Mello Costa.