ORCID Profile
0000-0002-7957-2305
Current Organisation
Tokyo Medical and Dental University
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Publisher: Elsevier BV
Date: 09-2013
DOI: 10.1016/J.JDENT.2013.06.002
Abstract: This study evaluated the inhibitory effect of proanthocyanidin (PA), a natural collagen cross-linker, on soluble and matrix-bound proteases, which are responsible for progressive degradation of exposed collagen fibrils within the hybrid layer and resin-dentine bond failure over time. The inhibitory effects of PA (1%, 2%, 3%, 4.5% and 6%) on soluble recombinant matrix metalloproteinases (MMP-2, -8 and -9) and cysteine cathepsins (cathepsin B and K) were evaluated using MMP and cysteine cathepsins fluorometric assay kits. Chlorhexidine (CHX) was used as an inhibitor control. The effect of PA on endogenous matrix-bound proteases was examined by determining the change in dry mass of demineralized dentine beams and solubilized collagen peptides over 30 days. Two-way ANOVA and Tukey multiple comparison tests were used to analyze the effect of PA and proteases on the percentage inhibition of soluble proteases (α=0.05). Kruskal-Wallis one-way ANOVA and Dunn's multiple comparison tests were used to analyse the effect of PA on loss of dry mass and hydroxyproline content over time (α=0.05). Proanthocyanidin inactivated more than 90% of soluble recombinant MMP-2, -8 and -9 and around 75-90% of cysteine cathepsin B and K, which was significantly higher than CHX (P<0.05). The inhibition of endogenous proteases by PA increased in a dose-dependent manner. The loss of dry mass and hydroxyproline release in the medium over time was the lowest in dentine beams pretreated with PA<CHX<control (P<0.05). Proanthocyanidin exhibited both dentine MMP and cysteine cathepsins inhibition, which was higher than chlorhexidine.
Publisher: MDPI AG
Date: 29-04-2018
DOI: 10.3390/IJMS19051322
Publisher: Elsevier BV
Date: 07-2020
Publisher: Elsevier BV
Date: 07-2003
DOI: 10.1016/S0109-5641(02)00087-8
Abstract: This study was to examine the effect of artificial saliva contamination on pH change of the dentin surface and the micro-shear bond strength (MSBS) of the two bonding systems to contaminated dentin. Fifty-six human dentin disks were tested with two resin bonding systems: a self-etching primer system, Clearfil SE Bond (Kuraray Medical Inc., Tokyo, Japan), and a one-bottle adhesive system, Single Bond (3M-ESPE, St. Paul, MN). Dentin surfaces were conditioned with the self-etching primer (primer) or phosphoric acid (etchant) and ided into four groups: conditioning without contamination (conditioning), contamination with artificial saliva (contamination), re-conditioning the contaminated dentin (re-conditioning), water-rinsing the contaminated dentin and re-conditioning (rinsing and re-conditioning). The pH change on the dentin surface was measured using a pH-imaging microscope (SCHEM-100, Horiba Ltd, Kyoto, Japan) to estimate the acid-base characteristics of the conditioned and contaminated dentin surface. The MSBS to the dentin was examined after storage in distilled water at 37 degrees C for 1 week. The pH of intact dentin surfaces was 6.9. Conditioning with the primer and etchant decreased the pH to 5.4 and 5.9, respectively. Saliva contamination increased the pH slightly, and re-conditioning decreased the pH again. The MSBS of the two bonding systems decreased after contamination. Re-priming restored the MSBS to control values, while re-etching did not. Rinsing and re-priming produced a reduction in MSBS, and rinsing and re-etching did not increase the MSBS. The conditioning and saliva contamination changed the pH value of dentin surface. The MSBS decreased after contamination however, re-priming with SE Bond primer was an adequate treatment to restore the bond strength.
Publisher: Japanese Society for Dental Materials and Devices
Date: 28-01-2019
DOI: 10.4012/DMJ.2018-008
Abstract: The objective was to evaluate the effect of application of silver diamine fluoride (SDF) on discoloration of demineralized dentin over time. Dentin specimens were ided into four groups according to time of dentin demineralization. A 38% SDF solution was then applied to the dentin surfaces. Half of the specimens were placed in light-proof boxes while the remainder were exposed to light. Both groups were maintained at 37˚C. Color change was determined using a spectrophotometer at different time intervals. SEM/EDS analysis were also undertaken. The 13 h EDTA demineralized group showed the highest values for color change among different time intervals, with the control being the lowest. The light exposed groups showed more color change compared to the unexposed groups. We concluded that the degree of dentin demineralization leads to a significant increase of the rate of dentin color change after application of SDF.
Publisher: Elsevier BV
Date: 04-2018
DOI: 10.1111/IDJ.12320
Abstract: To review the evidence regarding the mechanisms of silver diamine fluoride (SDF) for arresting caries. A literature search was conducted using the keywords silver diamine fluoride, and its alternative names, in seven databases: PubMed, Embase and Scopus (English) China National Knowledge Infrastructure (Chinese) Bilioteca Virtual em Saude (Portuguese) Biblioteca Virtual en Salud Espana (Spanish) and Ichushi-Web (Japanese). The titles and abstracts were screened. Full texts were retrieved for publications that studied mechanisms of actions of SDF, including its effects on remineralisation of carious lesions and on cariogenic bacteria. A total of 1,123 publications were identified. Twenty-nine articles were included and they investigated the effect of SDF on cariogenic bacteria and dental hard tissues. Eleven studies investigated the antibacterial properties of SDF. They found that SDF was bactericidal to cariogenic bacteria, mainly Streptococcus mutans. It inhibited the growth of cariogenic biofilms on teeth. Twenty studies reported the remineralisation of demineralised enamel or dentine by SDF. They found that mineral loss of demineralised enamel and dentine was reduced after SDF treatment. A highly mineralised surface rich in calcium and phosphate was formed on arrested carious lesions. Four studies examined the effect of SDF on dentine collagen. They found that SDF inhibited collagenases (matrix metalloproteinases and cysteine cathepsins) and protected dentine collagen from destruction. SDF is a bactericidal agent and reduces the growth of cariogenic bacteria. It inhibits demineralisation and promotes the remineralisation of demineralised enamel and dentine. It also h ers degradation of the dentine collagen.
Publisher: Springer Science and Business Media LLC
Date: 23-09-2020
No related grants have been discovered for Noriko Hiraishi.