ORCID Profile
0000-0003-1617-9220
Current Organisations
University of Oxford
,
University of Waterloo
,
University of Cambridge
,
Queen's University
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Publisher: American Chemical Society (ACS)
Date: 25-08-2021
Publisher: AIP Publishing
Date: 23-09-2021
DOI: 10.1063/5.0063258
Abstract: Incorporation of fluorescent proteins into biochemical systems has revolutionized the field of bioimaging. In a bottom-up approach, understanding the photophysics of fluorescent proteins requires detailed investigations of the light-absorbing chromophore, which can be achieved by studying the chromophore in isolation. This paper reports a photodissociation action spectroscopy study on the deprotonated anion of the red Kaede fluorescent protein chromophore, demonstrating that at least three isomers–assigned to deprotomers–are generated in the gas phase. Deprotomer-selected action spectra are recorded over the S1 ← S0 band using an instrument with differential mobility spectrometry coupled with photodissociation spectroscopy. The spectrum for the principal phenoxide deprotomer spans the 480–660 nm range with a maximum response at ≈610 nm. The imidazolate deprotomer has a blue-shifted action spectrum with a maximum response at ≈545 nm. The action spectra are consistent with excited state coupled-cluster calculations of excitation wavelengths for the deprotomers. A third gas-phase species with a distinct action spectrum is tentatively assigned to an imidazole tautomer of the principal phenoxide deprotomer. This study highlights the need for isomer-selective methods when studying the photophysics of biochromophores possessing several deprotonation sites.
Publisher: American Chemical Society (ACS)
Date: 16-09-2019
DOI: 10.1007/S13361-019-02332-1
Abstract: The preferential solvation behavior for eight different derivatives of protonated quinoline was measured in a tandem differential mobility spectrometer mass spectrometer (DMS-MS). Ion-solvent cluster formation was induced in the DMS by the addition of chemical modifiers (i.e., solvent vapors) to the N
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1CP02120F
Abstract: Differential mobility spectrometry is used to separate prototropic isomers of para -aminobenzoic acid prior to laser spectroscopic investigation.
Publisher: American Chemical Society (ACS)
Date: 02-01-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1CP02688G
Abstract: Differential mobility spectrometry is used to separate prototropic isomers of protonated adenine prior to laser spectroscopic investigation.
Publisher: American Chemical Society (ACS)
Date: 23-09-2022
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Scott Hopkins.