ORCID Profile
0000-0003-3846-7303
Current Organisations
Commonwealth Scientific and Industrial Research Organisation
,
CSIRO
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Publisher: Frontiers Media SA
Date: 19-08-2020
Publisher: Universidade Estadual de Maringa
Date: 2018
Publisher: Elsevier BV
Date: 04-2021
Publisher: Elsevier BV
Date: 2023
DOI: 10.1016/J.BONE.2022.116606
Abstract: Micro-computed tomography (microCT) offers a three-dimensional (3D), high-resolution technique for the visualisation and analysis of bone microstructure. Using contrast-enhanced microCT, this capability has been expanded in recent studies to include cartilage morphometry and whole joint measures, known together as quantitative morphometric analysis (QMA). However, one of the main challenges in quantitative analysis of joint images is sensitivity to joint pose and alignment, which may influence measures related to both joint space and joint biomechanics. Thus, this study proposes a novel microCT imaging protocol for reproducible and efficient QMA of in situ mouse tibio-femoral joint. This work consists of two parts: an in situ diffusion kinetics study for a known cationic iodinated contrast agent (CA4+) for QMA of the cartilage, and a joint positioning and image processing workflow for whole joint QMA. In the diffusion kinetics study, 8 mice were injected at both of their tibio-femoral joints with distinct CA4+ concentrations and diffusion times. The mice were scanned at different time points after injection, and evaluated using attenuation and cartilage QMA measures. Results show that cartilage segmentation and QMA could be performed for CA4+ solution at a concentration of 48 mg/ml, and that reliable measurement and quantification of cartilage were achieved after 5 min of diffusion following contrast agent injection. We established the joint positioning and image processing workflow by developing a novel positioning device to control joint pose during scanning, and a spherical harmonics-based image processing workflow to ensure consistent alignment during image processing. Both legs of seven mice were scanned 10 times, 5 prior to receiving CA4+ and 5 after, and evaluated using whole joint QMA parameters. Joint QMA evaluation of the workflow showed excellent reproducibility intraclass correlation coefficients ranged from 0.794 to 0.930, confirming that the imaging protocol enables reproducible and efficient QMA of joint structures in preclinical models, and that contrast agent injection did not cause significant alteration to the measured parameters.
Publisher: Wiley
Date: 19-05-2022
DOI: 10.1002/JOR.25361
Abstract: Osteoarthritis (OA) is a chronic joint disease that causes disability and pain. The osteochondral interface is a gradient tissue region that plays a significant role in maintaining joint health. It has been shown that during OA, increased neoangiogenesis creates porous channels at the osteochondral interface allowing the transport of molecules related to OA. Importantly, the connection between these porous channels and the early stages of OA development is still not fully understood. Microcomputed tomography (microCT) offers the ability to image the porous channels at the osteochondral interface, however, a contrast agent is necessary to delineate the different X‐ray attenuations of the tissues. In this study BaYbF 5 ‐SiO 2 nanoparticles are synthesized and optimized as a microCT contrast agent to obtain an appropriate contrast attenuation for subsequent segmentation of structures of interest, that is, porous channels, and mouse subchondral bone. For this purpose, BaYbF 5 nanoparticles were synthesized and coated with a biocompatible silica shell (SiO 2 ). The optimized BaYbF 5 ‐SiO 2 27 nm nanoparticles exhibited the highest average microCT attenuation among the biocompatible nanoparticles tested. The BaYbF 5 ‐SiO 2 27 nm nanoparticles increased the mean X‐ray attenuation of structures of interest, for ex le, porous channel models and mouse subchondral bone. The BaYbF 5 ‐SiO 2 contrast attenuation was steady after diffusion into mouse subchondral bone. In this study, we obtained for the first time, the average microCT attenuation of the BaYbF 5 ‐SiO 2 nanoparticles into porous channel models and mouse subchondral bone. In conclusion, BaYbF 5 ‐SiO 2 nanoparticles are a potential contrast agent for imaging porous channels at the osteochondral interface using microCT.
Location: Australia
Location: No location found
No related grants have been discovered for Dr Mateus Oliveira Silva.