ORCID Profile
0000-0001-8239-0716
Current Organisation
Mahidol University Faculty of Dentistry
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Publisher: Wiley
Date: 22-11-2019
DOI: 10.1111/JICD.12371
Abstract: The aim of the present study was to investigate bacterial leakage and marginal adaptation of bioceramic apical plugs. Extracted human mandibular premolars were prepared to simulate open apex using No. 4 Peeso reamer in retrograde direction. In total, 150 specimens were ided into 10 groups by obturation with five bioceramics in two thicknesses. Groups 1, 3, 5, 7, and 9 were obturated with ProRootMTA, Biodentine, TotalFill BC RRM paste, TotalFill BC RRM putty, and RetroMTA at 3 mm, and groups 2, 4, 6, 8, and 10 were obturated with the same materials at 4 mm. Ten specimens in each group were evaluated for bacterial leakage of Enterococcus faecalis for 75 days. Five specimens from each group were sectioned to investigate the gap area under scanning electron microscope. The 3- and 4-mm Biodentine and TotalFill BC RRM putty groups and the 4-mm ProRootMTA group exhibited less bacterial leakage and lower mean percentage of gap area than those of the other groups. TotalFill BC RRM paste showed the highest leakage for both the 3- and 4-mm groups. The 3- and 4-mm Biodentine and TotalFill BC RRM putty groups and the 4-mm ProRootMTA group exhibited the best sealing ability and marginal adaptation of apical plugs.
Publisher: Elsevier BV
Date: 10-2012
DOI: 10.1016/J.JOEN.2012.06.028
Abstract: There are numerous challenges in treating immature permanent teeth with a diagnosis of pulp necrosis. Three general treatment options are calcium hydroxide apexification, mineral trioxide aggregate (MTA) apexification, and revascularization. The objective of this retrospective study was to evaluate radiographic and clinical outcomes of immature teeth treated with 1 of these 3 methods. Clinical outcome data and radiographs were collected from 61 cases (ie, 22 calcium hydroxide apexification cases, 19 MTA apexification cases, and 20 revascularization cases). Both tooth survival and clinical success rates were analyzed. In addition, the preoperative and recall radiographs were analyzed to calculate the percentage increase in root width and length. The percentage change of root width was significantly greater in the revascularization group (28.2%) compared with the MTA apexification (0.0%) and calcium hydroxide apexification groups (1.5%). In addition, the percentage increase of root length was significantly greater in the revascularization group (14.9%) compared with the MTA (6.1%) and calcium hydroxide apexification groups (0.4%). Moreover, the survival rate of the revascularization-treated teeth (100%) and MTA apexification-treated teeth (95%) were greater than the survival rates observed in teeth treated with calcium hydroxide (77.2%). In this study, revascularization was associated with significantly greater increases in root length and thickness in comparison with calcium hydroxide apexification and MTA apexification as well as excellent overall survival rates.
Publisher: Wiley
Date: 02-2013
DOI: 10.1111/J.2041-1626.2012.00143.X
Abstract: In the present study, we evaluate the efficiency of four kinds of oil as solvents for softening gutta-percha in root canal retreatment procedures. The performance of grapefruit, tangerine, lime, and lemon oils specifically were evaluated and compared to chloroform. Eighty-four cylinder-shaped glass tubes (5 × 30 mm) were filled with gutta-percha. A total of 0.1 mL solvent was added to the upper part of the glass tubes. After 5 min, the spreader attached to the universal testing machine was moved downward through gutta-percha with header speed of 5 mm/min into a depth of 5 mm. An initial depth of spreader penetration without force produced was recorded in millimeters. The load used to penetrate the spreader into the 5-mm depth was recorded in Newton. The results of the surface-dissolving depth and maximum force used to penetrate the spreader to 5 mm revealed that chloroform was significantly the best solvent in softening gutta-percha (P < 0.05), followed by grapefruit oil and tangerine oil, while the least effective solvents were lime oil and lemon oil. Under the conditions of the present study, grapefruit oil and tangerine oil performed best as gutta-perch solvents among four natural oils.
Publisher: Elsevier BV
Date: 08-2018
DOI: 10.1016/J.JOEN.2018.03.014
Abstract: In regenerative endodontic treatment (RET), practitioners favor the placement of bioceramics as sealing materials over blood clots. It is important to understand the interaction between sealing material and cells in the root canal. The purpose of this study was to compare the effectiveness of various bioceramic materials (ProRoot MTA [Dentsply, Tulsa, OK], Biodentine [Septodont, Saint-Maur-des-Fossés, France], and RetroMTA [BioMTA, Seoul, Korea]) as sealing materials in RET for the proliferation and differentiation of stem cells from the apical papilla (SCAPs). SCAPs were seeded at 20,000 cells/well and cultured with soluble agents of testing materials through a transwell culture plate. The proliferation of SCAPs was investigated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on days 1, 3, 7, and 14 of testing. Alizarin red staining and quantitative real-time polymerase chain reaction were used for SCAP differentiation at different time points (1, 7, 14, and 21 days). The odontoblast genes expressed are dentin matrix acidic phosphoprotein 1, dentin sialophosphoprotein, osteocalcin, and matrix extracellular phosphoglycoprotein, which were used in this study. The SCAPs were cultured in odonto/osteogenic induction medium and also contacted soluble agents from the testing materials. All 3 tested biomaterials induced SCAP proliferation. The Biodentine, ProRootMTA, and RetroMTA groups showed significant SCAP proliferation on days 7 and 14 compared with the control. In regard to odontoblastic differentiation, only Biodentine showed positive alizarin red staining. The highest expressions of dentin matrix acidic phosphoprotein 1, dentin sialophosphoprotein, and matrix extracellular phosphoglycoprotein were found on day 21 in the Biodentine group. The expression of osteocalcin was found to be significant on day 7. Biodentine, ProRootMTA, and RetroMTA can induce SCAP proliferation. Biodentine induced significant SCAP differentiation among the 3 materials.
Publisher: Elsevier BV
Date: 11-2018
DOI: 10.1016/J.JOEN.2018.08.001
Abstract: The outcomes of an immature tooth with necrotic pulp treated with regenerative endodontic procedures (REPs) were assessed clinically and radiographically. Root maturation is an important outcome of REPs, and several radiographic measurement methods have been used to measure this. The aim of this study was to compare radiographic measurement methods, measuring root maturation in immature teeth with necrotic pulp treated with REPs. Seventy-one radiographic images of REP cases were measured and compared using 3 radiographic measurement methods described by Bose et al (2009), Alobaid et al (2014), and Flake et al (2014). The intraclass correlation coefficient values were evaluated using the intra- and interobserver reliability test and the effect of the stage of root development. The intra- and interobserver reliability for Alobaid et al's method and Flake et al's method were slightly higher than Bose et al's method as quantified by the intraclass correlation coefficient without a significant difference (P > .05). The stage of root development did not affect the reliability of the measurement methods. A high level of agreement was found among the 3 stages of root development for all 3 quantitative radiographic measurement methods. All 3 quantitative radiographic measurement methods exhibited high agreement regarding reliability. The stage of root development did not have an impact on the reliability of the measurement methods.
Publisher: Elsevier BV
Date: 03-1999
Publisher: Wiley
Date: 20-12-2021
DOI: 10.1111/AEJ.12604
Abstract: This study compared the percentage reduction of total obturation materials, gutta‐percha and sealer, using two rotary file systems with/without solvent in retreating C‐shaped root canals and to evaluate the effect of the solvent on instrument separation. C‐shaped root canals were scanned using micro‐CT to identify the root canal morphology and randomly assigned to four groups ( n = 12). Retreatment was performed using ProTaper Universal Retreatment (PTUR) or XP‐endo systems with/without solvent. The number of separated instruments were recorded. The s les were scanned pre‐ and post‐retreatment. The percentage reduction of total obturation materials, gutta‐percha and sealer, were calculated. The percentage reduction of gutta‐percha in the PTUR without solvent group was significantly lower than the other groups ( P = 0.018). The number of separated instruments in the solvent groups were significantly lower than the no solvent groups ( P 0.05). Solvent increased gutta‐percha removal, when using PTUR and decreased the occurrence of separated instruments for both systems.
Publisher: Elsevier BV
Date: 03-2019
DOI: 10.1016/J.JOEN.2018.10.010
Abstract: In regenerative endodontic procedures (REPs), a blood clot acts as a natural scaffold for regenerating dental pulp tissue. In current protocols, 17% EDTA is recommended for liberating growth factors from root dentin. Although EDTA affects clot formation in periodontal studies, the anticoagulant effect of EDTA has not been shown in REPs. Therefore, this study aimed to evaluate the effects of 17% EDTA on the characteristics and fiber density of blood clots using in vitro dentin blocks. The roots of 35 human mandibular premolars were prepared to simulate open apices and irrigated with the following protocols: (1) normal saline solution (NSS), (2) EDTA (1 minute) + NSS (E1N), (3) EDTA (5 minutes) + NSS (E5N), (4) EDTA (1 minute) (E1), and (5) EDTA (5 minutes) (E5). The roots were split, and human blood was placed. The characteristics and fibrin density of clots were observed using a scanning electron microscope. Fibrin densities in all irrigation groups were evaluated using ImageJ software (National Institutes of Health, Bethesda, MD) and statistically analyzed using the Friedman test and the Kruskal-Wallis test with Bonferroni adjustment. S les in the NSS, E1N, and E5N groups revealed denser fibers with an abundance of erythrocytes when compared with those in the E1 and E5 groups. Fiber densities in the E1 and E5 groups showed significantly lower values than those in the NSS, E1N, and E5N groups in all regions of roots (P < .05). No statistically significant difference at all levels was observed in all irrigation groups. A decrease in clot formation was affected by EDTA irrigation for 1 and 5 minutes. Final flushing with NSS could improve fibrin formation.
Publisher: Kare Publishing
Date: 2022
Publisher: Elsevier BV
Date: 04-2019
Publisher: Springer Science and Business Media LLC
Date: 13-06-2014
DOI: 10.1007/S00784-014-1256-8
Abstract: For dental treatment, dentin regeneration is required after a tooth injury with dental pulp exposure. The effects of the water-soluble matrix (WSM) extracted from the nacreous layer of the bivalve Pinctada maxima on human dental pulp cells in vitro were challenging and useful for clinical application. The biological activity of the STRO-1-positive human dental pulp cells in response to WSM compared to Dulbecco's modified Eagle medium (DMEM) as a normal control was monitored. The cell survival rate was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Proteomic profiles among inducers and noninducers with time dependency were compared by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with liquid chromatography-tandem mass spectrometry (GeLC-MS/MS). The human dental pulp cells cultured in nacreous WSM exhibited higher relative cell viability than those in DMEM with similar morphological appearance. Significant changes were found in the relative abundance of 44 proteins in cells after exposure to WSM for 2 weeks. They play a role in cell adhesion, cell proliferation, metabolic process, signal transduction, stress response, transcription, translation, and transport. These results indicate that WSM of P. maxima has the ability to induce proliferation of human dental pulp cells. This finding initiated the study to evaluate the suitability of nacre as biomaterial for dentistry.
Publisher: Elsevier BV
Date: 2001
DOI: 10.1016/S0300-5712(00)00040-3
Abstract: The aim of this study was to compare two techniques of measuring cuspal deformation under occlusal load: Direct Current Differential Transformers (DCDTs) and strain gauges. The study investigated the relative dependence of the two techniques on the vertical orientation of the tooth in relation to direction of loading and differences in the patterns of change in response to a cavity preparation sequence. Strain gauges and DCDTs were attached to the buccal and lingual surfaces of extracted maxillary premolars and mandibular molars. Premolars were subjected to simulated occlusal loads at varying vertical angulations before and after MOD cavity preparation. Molars were tested at progressive stages of cavity preparation. Cuspal deformation was recorded as linear cusp displacement (in microm, using DCDTs) and as cuspal strains (using strain gauges) and relative stiffness. Strain gauges were much less sensitive than linear displacement devices to vertical orientation of teeth, and computation of relative stiffness further reduced the effect of angulation. Strain gauges are much easier to use experimentally. DCDTs required precise three-dimensional adjustments for the testing of cuspal deformation. Patterns of change in cuspal flexure following cavity preparation were very different using the two techniques. Use of both devices simultaneously yielded much more information than when used alone, and showed that cusps do not deform as simple cantilever beams.
Publisher: Wiley
Date: 12-1998
Publisher: Wiley
Date: 12-1998
Publisher: Wiley
Date: 08-1998
Publisher: Wiley
Date: 05-10-2022
DOI: 10.1111/AEJ.12696
Abstract: This study compared the gap volume and sealer penetration in C-shaped root canals prepared with adaptive core rotary files and obturated with cold hydraulic compaction using calcium-silicate sealer, warm vertical hybrid compaction, or lateral compaction using epoxy-resin sealer. Thirty-six extracted mandibular molars with pulpal floor configuration Types I and III were used. The teeth were prepared using XP-Shaper and XP-Finisher and obturated with: group 1: cold hydraulic compaction/calcium silicate, group 2: warm vertical hybrid compaction/epoxy resin, or group 3: lateral compaction/epoxy resin. The gap volume was evaluated using μ-CT. The sealer penetration depth and area were evaluated using confocal laser scanning microscopy. The gap volume in groups 1, 2, and 3 was 0.82%, 0.24%, 0.80%, respectively, which were not significantly different (p > 0.05). The gap volumes in the obturated C-shaped canals were not significantly different among the CHC/CSBS, WHC/ERS, or LC/ERS groups. CHC/CSBS was the most convenient technique.
Publisher: Springer Science and Business Media LLC
Date: 09-01-2015
DOI: 10.1007/S40368-014-0163-7
Abstract: This was to compare the effectiveness of different preparations and delivery techniques of calcium hydroxide paste as an intracanal medicament in primary molar root canals. Extracted lower primary second molars roots (72) were randomly assigned to the four experimental groups. After complete root canal preparation with TF rotary nickel titanium up to .06/30 file, a mix of calcium hydroxide/ distilled water was delivered by lentulo spiral (group 1) or a syringe (group 2). UltraCal(®) XS was placed by lentulo spiral (group 3) and a syringe (group 4). Digital radiographs were taken in both buccal and proximal directions before and after calcium hydroxide placement to evaluate the quality of filling. The mean percentages of filled calcium hydroxide area by subtracted digital radiograph, and the quality assessments between four experimental groups, were compared by using Two-way ANOVA and Fisher's exact test respectively. A p value of less than 0.05 was considered statistical significance. There was no statistically significant difference in the mean percentages of the filled calcium hydroxide area among different preparations and delivery techniques (p ≥ 0.05). The syringe technique had statistically significant fewer of air inclusions than when using the lentulo spiral with the mix of calcium hydroxide group (p < 0.05). The completeness of obturation and the quality of filling at apical part were not different among groups (p ≥ 0.05). Syringe technique was a simpler handling method that presented similar filling quality but fewer porosities than lentulo spiral technique in primary molar root canals.
Publisher: Wiley
Date: 13-08-2018
DOI: 10.1111/AEJ.12302
Abstract: The aim of this study was to investigate the post-treatment apical periodontitis in endodontically treated teeth related to the technical quality of root fillings and restorations in Thai population. Full-mouth periapical radiographs from Thai patients attending the Faculty of Dentistry, Mahidol University, Thailand were examined. The periapical status, quality of root fillings and coronal restorations, existence of intraradicular post and its relationship to the root fillings were evaluated. Among 1586 patients, 37.4% presented endodontically treated teeth. The total number of endodontically treated teeth was 1511, and 35% presented apical periodontitis. Combined data for root filling and coronal restoration qualities revealed significantly better outcome in teeth with adequate qualities of both parameters than the other combinations (P < 0.001). The prevalence of post-treatment apical periodontitis among Thai population was relatively high and significantly associated with substandard technical quality of root fillings and restorations.
Publisher: Elsevier BV
Date: 07-2001
DOI: 10.1016/S0300-5712(01)00018-5
Abstract: The purpose of this study was to investigate the extent of progressive cuspal displacement during prolonged occlusal loading (either continuous or cyclic) and delayed cuspal recovery following removal of the load. Extracted maxillary premolars with extensive MOD cavity plus endodontic access were subjected to an occlusal load applied to both cusps, followed by a recovery period. Two types of loading were applied: (a) continuous load of 100, 200 and 300 N for 0.1--1000 s (b) cyclic loading of 300 N at 0.83 Hz for 1--1000 cycles. Cuspal displacement during loading and subsequent recovery were measured with an extensometer attached to both cusps. Continuous loading led to progressive cuspal displacement that was both time- and load-dependent. After removal of the load, complete recovery from cuspal deformation was prolonged up to 20 min, also in a time- and load-dependent manner. Cyclic loading resulted in a cumulative increase in cusp displacement but to only a very small extent (approximately 1 microm after 1000 cycles). Progressive cuspal displacement and delayed recovery occur with prolonged continuous or cyclic occlusal loading. Continuous loading (e.g. as in clenching) is potentially more damaging than cyclic loading (as in chewing).
Publisher: Elsevier BV
Date: 09-2002
DOI: 10.1016/S0109-5641(01)00074-4
Abstract: The aim of this study was to investigate the creep, stress relaxation and strain rate behavior of human root dentin under compressive loading. Cylindrical root dentin s les of 3.5mm outer diameter, 1.5mm internal canal diameter and 6-10mm long were prepared from freshly extracted teeth. The s les were tested in a closed-loop servohydraulic testing machine at constant load or displacement, and varied strain rate. In vivo strain rates were estimated using strain gauges bonded to human teeth. A family of creep curves, determined at different loads within dentin's elastic region, was found to be consistent with a material having linear viscoelastic behavior. A positive correlation (r(2)=0.79, P<0.001) was found between creep rate and stress. Young's modulus (E) was found to be a function of the strain rate with rates of loading in the range 10-500,000Ns(-1). Loading at constant displacement showed stress to be a decreasing function of time (i.e. stress relaxation). Dentin showed linear-viscoelastic behavior under various conditions of compressive loading. Time dependent properties of dentin should be taken into account in restorative dentistry.
Publisher: Wiley
Date: 27-07-2015
DOI: 10.1111/JICD.12177
Abstract: The aim of the present study was to compare cytotoxicity and migration ability of L929 on medical-grade calcium sulfate dihydrate (MCS) with commercial calcium sulfate dihydrate (CCS). S les of both freshly-mixed and set states of MCS and CCS were extracted in culture medium and tested for cytotoxicity according to International Organization of Standardization 10993-5:2009. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used, and the percentage of cell viability was calculated. Thirty petri dishes were ided into three equal groups: polystyrene (control), CCS, and MCS. In each petri dish, triplicate wells were made on the agarose overlay on the material. The central well was occupied with L929 cell suspension, one well was filled with platelet-derived growth factor-BB, and the remaining well with Hanks' Balanced Salt Solution. After incubation, the migration distances were measured and the corrected migration distances were calculated. The final step involved scanning electron microscopy (SEM). The CCS had significantly less cell viability than MCS at a high extracted concentration (P < 0.001) in both s les. The mean corrected migration of MCS was significantly greater than that of CCS (P < 0.05). SEM showed that L929 cells on MCS exhibited an elongated spindle shape. MCS was less cytotoxic and provided greater migration of L929 fibroblasts compared with CCS.
Publisher: Springer Science and Business Media LLC
Date: 15-02-2023
No related grants have been discovered for Jeeraphat Jantarat.