Publication
In vitro effect of 20% N-acetylcysteine on the viscosity of normal canine bile.
Publisher:
American Veterinary Medical Association (AVMA)
Date:
2019
DOI:
10.2460/AJVR.80.1.74
Abstract: OBJECTIVE To evaluate the in vitro effect of 20% N -acetylcysteine (NAC) on the viscosity of normal canine bile. ANIMALS Bile s les obtained from 10 adult dogs euthanized for reasons unrelated to biliary disease. PROCEDURES Each s le was centrifuged to remove particulates, then ided into 3 aliquots. One aliquot remained untreated (control). Each of the other aliquots was diluted 1:4 with 20% NAC or sterile water. The viscosity of all s les was measured with a rotational viscometer at 25°C. Viscosity of control s les was measured immediately after centrifugation and at 1 and 24 hours after treatment application to the diluted s les. Viscosity of diluted s les was measured at 1 and 24 hours after treatment application. RESULTS Mean viscosity differed significantly among the 3 groups at both 1 and 24 hours after treatment application. Relative to control s les, the addition of NAC and sterile water decreased the viscosity by approximately 3.35 mPa·s (95% confidence interval [CI], 1.58 to 5.12 mPa·s) and 2.74 mPa·s (95% CI, 1.33 to 4.14 mPa·s), respectively. Mean viscosity of the NAC-treated s les was approximately 0.61 mPa·s (95% CI, 0.21 to 1.01 mPa·s) less than that for the sterile water–treated s les. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that in vitro dilution of canine bile 1:4 with 20% NAC significantly decreased the viscosity of the resulting mixture. Further research is necessary to determine whether NAC is a safe and effective noninvasive treatment for dogs with persistent biliary sludge or gallbladder mucoceles.