ORCID Profile
0000-0002-9898-054X
Current Organisations
Shanghai Childrens Medical Center Affiliated to Shanghai Jiaotong University School of Medicine
,
Pediatric Translational Medicine Institute, Shanghai Jiao Tong University School of Medicine
,
Shanghai Pediatric Congenital Heart Disease Institute
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Related Links
Publications
Massively parallel sequencing identifies the gene Megf8 with ENU-induced mutation causing heterotaxy
Publisher: Proceedings of the National Academy of Sciences
Date: 03-03-2009
Abstract: Forward genetic screens with ENU ( N -ethyl- N -nitrosourea) mutagenesis can facilitate gene discovery, but mutation identification is often difficult. We present the first study in which an ENU- induced mutation was identified by massively parallel DNA sequencing. This mutation causes heterotaxy and complex congenital heart defects and was mapped to a 2.2-Mb interval on mouse chromosome 7. Massively parallel sequencing of the entire 2.2-Mb interval identified 2 single-base substitutions, one in an intergenic region and a second causing replacement of a highly conserved cysteine with arginine (C193R) in the gene Megf8. Megf8 is evolutionarily conserved from human to fruit fly, and is observed to be ubiquitously expressed. Morpholino knockdown of Megf8 in zebrafish embryos resulted in a high incidence of heterotaxy, indicating a conserved role in laterality specification. Megf8 C193R mouse mutants show normal breaking of symmetry at the node, but Nodal signaling failed to be propagated to the left lateral plate mesoderm. Videomicroscopy showed nodal cilia motility, which is required for left–right patterning, is unaffected. Although this protein is predicted to have receptor function based on its amino acid sequence, surprisingly confocal imaging showed it is translocated into the nucleus, where it is colocalized with Gfi1b and Baf60C, two proteins involved in chromatin remodeling. Overall, through the recovery of an ENU-induced mutation, we uncovered Megf8 as an essential regulator of left–right patterning.
Wdpcp, a PCP Protein Required for Ciliogenesis, Regulates Directional Cell Migration and Cell Polarity by Direct Modulation of the Actin Cytoskeleton
Publisher: Public Library of Science (PLoS)
Date: 26-11-2013
Religiosity and Subjective Well-Being Among Old People: Evidence from a Transitional Country
Publisher: Springer Science and Business Media LLC
Date: 24-11-2016
Exploring the Genetic Basis for Congenital Heart Disease with Mouse Enu Mutagenesis
Publisher: Elsevier
Date: 2010
Related Organisations
National Heart Lung & Blood Institute
Location: United States of America
Shanghai Jiao Tong University School Of Medicine Affiliated Renji Hospital
Location: China
Shanghai Childrens Medical Center Affiliated To Shanghai Jiaotong University School Of Medicine
Location: China
Pediatric Translational Medicine Institute, Shanghai Jiao Tong University School Of Medicine
Location: China
Shanghai Pediatric Congenital Heart Disease Institute
Location: China
Related Funding Activities
No related grants have been discovered for Zhen Zhang.