ORCID Profile
0000-0001-6335-7208
Current Organisation
University of Saskatchewan
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Publisher: Oxford University Press (OUP)
Date: 29-08-2018
DOI: 10.1093/JAS/SKY265
Publisher: Canadian Science Publishing
Date: 12-2014
Abstract: Meale, S. J., Ding, S., He, M. L., Dugan, M. E. R., Ribeiro Jr. G. O., Alazzeh, A. Y., Holo, H., Harstad, O. M., McAllister, T. A. and Chaves, A. V. 2014. Effect of Propionibacterium freudenreichii on ruminal fermentation patterns, methane production and lipid biohydrogenation of beef finishing diets containing flaxseed oil in a rumen simulation technique. Can. J. Anim. Sci. 94: 685–695. The objectives of this study were to examine the effects of Propionibacterium freudenreichii (strain T54 PB) and flaxseed oil (FO) in a total mixed ration on ruminal fermentation, CH 4 production and fatty acid biohydrogenation in two artificial rumens (RUSITEC). The experiment consisted of 8 d of adaptation and 12 d of s le collection with four replicate fermenters per treatment. Treatments were: (1) CON (2) PB (3) FO (60 g kg −1 DM with autoclaved PB) (4) FOPB (60 g kg −1 DM with PB). Disappearance of DM (g kg −1 DM) and gas production (mL g −1 DM) were not affected by treatment (P .05). Inclusion of FOPB increased (P=0.01) total volatile fatty acid (VFA) production (mmol d −1 ), compared with CON and PB. The acetate:propionate ratio was reduced (P .001) in all treatments, compared with CON. Methane production (mL g −1 DM or mL g −1 DMD) was lowest (P .001) with PB (27.1%) however, FO (14.3%) and FOPB (19.3%) also reduced CH 4 compared with CON. Fatty acid profiles for PB were similar (P .05) to CON for most fatty acids. Concentrations of 18:3n-3 were greater (P .001) in FO and FOPB in both digesta and effluent, compared with CON. Propionibacterium freudenreichii had very little effect on ruminal biohydrogenation, but reduced CH 4 production under the current conditions as a result of increasing propionate production.
Publisher: Elsevier BV
Date: 06-2016
Publisher: Frontiers Media SA
Date: 17-09-2019
Publisher: CSIRO Publishing
Date: 2017
DOI: 10.1071/AN15878
Abstract: The objectives of the present study were to determine the effect of inoculating Propionibacterium freudenreichii subsp. shermanii ATCC 8262 (1 × 109 colony-forming units per vial) in a barley silage-based diet supplemented with flaxseed oil or rapeseed oil (60 g/kg DM), on in vitro proportions and yield of volatile fatty acids, methane production and fatty acid (FA) biohydrogenation. Total volatile fatty acid production (mM) and proportions of in idual FAs were not affected (P ≥ 0.10) by P. freudenreichii. Similarly, propionibacteria had little impact on FA biohydrogenation, resulting only in an increased accumulation (P 0.01) of C18:1 cis-15 (g/kg total FA) at 6 h of incubation, compared with the control (CON). Compared with the CON, an increased (P 0.01) accumulation of vaccenic acid was observed at 48 h in all oil-containing treatments, regardless of the oil type. Similarly, the apparent biohydrogenation of flaxseed oil resulted in an increased (P ≤ 0.04) accumulation of conjugated linoleic acid cis-9, trans-11, compared with all other treatments. Additionally, flaxseed oil produced a greater (P ≤ 0.01) accumulation of beneficial biohydrogenation intermediates (C18:2 trans-11, cis-15 C18:1 cis-15 and vaccenic acid), reflecting its ability to produce a more desirable FA profile than that of rapeseed oil or CON. The inability of P. freudenreichii subsp. shermanii ATCC 8262 to alter ruminal fermentation in a manner that lowered methane production, along with only minor effects on FA profiles through biohydrogenation, suggests that the biological activity of this strain was not realised under in vitro batch-culture conditions.
Publisher: Asian Australasian Association of Animal Production Societies
Date: 22-12-2014
Publisher: Elsevier BV
Date: 2015
Publisher: Cambridge University Press (CUP)
Date: 07-10-2001
DOI: 10.1017/S0007114513003206
Abstract: We hypothesised that the inclusion of glycerol in the forage diets of ruminants would increase the proportion of propionate produced and thereby decrease in vitro CH 4 production. This hypothesis was examined in the present study using a semi-continuous fermentation system (rumen simulation technique) fed a brome hay (8·5 g) and maize silage (1·5 g) diet with increasing concentrations (0, 50, 100 and 150 g/kg DM) of glycerol substituted for maize silage. Glycerol linearly increased total volatile fatty acids production ( P 0·001). Acetate production was quadratically affected ( P = 0·023) and propionate and butyrate production was linearly increased ( P 0·001). Glycerol linearly increased ( P = 0·011) DM disappearance from hay and silage. Crude protein disappearance from hay was not affected ( P = 0·789), but that from silage was linearly increased ( P 0·001) with increasing glycerol concentrations. Neutral-detergent fibre ( P = 0·040) and acid-detergent fibre ( P = 0·031) disappearance from hay and silage was linearly increased by glycerol. Total gas production tended to increase linearly ( P = 0·061) and CH 4 concentration in gas was linearly increased ( P 0·001) by glycerol, resulting in a linear increase ( P 0·001) in mg CH 4 /g DM digested. Our hypothesis was rejected as increasing concentrations of glycerol in a forage diet linearly increased CH 4 production in semi-continuous fermenters, despite the increases in the concentrations of propionate. In conclusion, this apparent discrepancy is due to the more reduced state of glycerol when compared with carbohydrates, which implies that there is no net incorporation of electrons when glycerol is metabolised to propionate.
Location: Canada
No related grants have been discovered for Gabriel Ribeiro.