ORCID Profile
0000-0001-6320-7189
Current Organisations
The University of Tennessee Knoxville
,
Shenzhen Institutes of Advanced Technology
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Publisher: IOP Publishing
Date: 28-04-2023
Abstract: Refractive disorder is the most prevalent cause of visual impairment worldwide. While treatment of refractive errors can bring improvement to quality of life and socio-economic benefits, there is a need for in idualization, precision, convenience, and safety with the chosen method. Herein, we propose using pre-designed refractive lenticules based on poly-NAGA-GelMA (PNG) bio-inks photo-initiated by digital light processing (DLP)-bioprinting for correcting refractive errors. DLP-bioprinting allows PNG lenticules to have in idualized physical dimensions with precision achievable to 10 µ m ( μ m). Material characteristics of PNG lenticules in tests included optical and biomechanical stability, biomimetical swelling and hydrophilic capability, nutritional and visual functionality, supporting its suitability as stromal implants. Cytocompatibility distinguished by morphology and function of corneal epithelial, stromal, and endothelial cells on PNG lenticules suggested firm adhesion, over 90% viability, phenotypic maintenance instead of excessive keratocyte-myofibroblast transformation. In-vitro immune response analyzed by illumina RNA sequencing in human peripheral blood mononuclear cells indicated that PNG lenticules activated type-2 immunity, facilitating tissue regeneration and suppressing inflammation. In-vivo performance assessed using intrastromal keratoplasty models in New Zealand white rabbits illustrated that implantation of PNG lenticules maintained stable optical pathway, induced controlled stromal bio-integration and regeneration, avoided complications such as stromal melt, interface scarring, etc, but exerted no adverse effects on the host. Postoperative follow-up examination on intraocular pressure, corneal sensitivity, and tear production remained unaffected by surgery up to 1-month post-implantation of PNG lenticules. DLP-bioprinted PNG lenticule is a bio-safe and functionally effective stromal implants with customizable physical dimensions, providing potential therapeutic strategies in correction of refractive errors.
Publisher: Elsevier BV
Date: 02-2018
DOI: 10.1016/J.COLSURFB.2017.11.070
Abstract: Although heterogeneous bone scaffolds have shown potential in bone defect repair, their capability of aiding bone regeneration need to be further enhanced. Strontium, one important trace element in bone, has a well-known favorable effect on bone repair. Here a strontium containing scaffold (CPB/PCL/Sr) based on superficially porous calcined porcine bone (CPB) was obtained straightforwardly by sequential coating of SrCl
Publisher: Wiley
Date: 09-01-2019
Abstract: For the 3D printing of bioscaffolds, the importance of a suitable bioink cannot be overemphasized. With excellent printability and biocompatibility, alginate (Alg) is one of the most used bioinks. However, its bioinert nature and insufficient mechanical stability, due to only crosslinking via cation interactions, hinder the practical application of Alg‐based bioinks in the in idualized therapy of tissue defects. To overcome these drawbacks, for the first time, an ε‐polylysine (ε‐PL)‐modified Alg‐based bioink (Alg/ε‐PL) is produced. The introduction of ε‐PL improves the printability of the Alg‐based bioink due to increasing electrostatic interactions, which enhances the self‐supporting stability of the as‐printed scaffolds. The presence of the functional crosslinking –COOH and –NH 2 groups in Alg and ε‐PL under mild conditions further enhances the mechanical stability of the scaffolds, far exceeding that of Alg/Ca 2+ scaffolds. The surface charge of the prepared scaffolds is finely tuned by the feed ratio of Alg to ε‐PL and postimmobilization of different quantities of additional ε‐PL, with a view to enhancing cell adhesion and further biofunctionalization. The results indicate that chondroitin sulfate, an extracellular matrix component, and vascular endothelial growth factor can be successfully applied to biofunctionalize the scaffolds via electrostatic adsorption for enhanced biological activity.
Location: United States of America
Location: No location found
No related grants have been discovered for Changshun Ruan.