Publication
Preventive Inositol Hexaphosphate Extracted from Rice Bran Inhibits Colorectal Cancer through Involvement of Wnt/β-Catenin and COX-2 Pathways
Publisher:
Hindawi Limited
Date:
2013
DOI:
10.1155/2013/681027
Abstract: Nutritional or dietary factors have drawn attention due to their potential as an effective chemopreventive agent, which is considered a more rational strategy in cancer treatment. This study was designed to evaluate the effect of IP 6 extracted from rice bran on azoxymethane- (AOM-) induced colorectal cancer (CRC) in rats. Initially, male Sprague Dawley rats were ided into 5 groups, with 6 rats in each group. The rats received two intraperitoneal ( i.p. ) injections of AOM in saline (15 mg/kg body weight) over a 2-week period to induce CRC. IP 6 was given in three concentrations, 0.2% (w/v), 0.5% (w/v), and 1.0% (w/v), via drinking water for 16 weeks. The deregulation of the Wnt/ β -catenin signaling pathway and the expression of cyclooxygenase (COX)-2 have been implicated in colorectal tumorigenesis. β -Catenin and COX-2 expressions were analysed using the quantitative RT-PCR and Western blotting. Herein, we reported that the administration of IP 6 markedly suppressed the incidence of tumors when compared to the control. Interestingly, the administration of IP 6 had also markedly decreased β -catenin and COX-2 in colon tumors. Thus, the downregulation of β -catenin and COX-2 could play a role in inhibiting the CRC development induced by IP 6 and thereby act as a potent anticancer agent.