ORCID Profile
0000-0002-3996-0856
Current Organisation
Universidade de Brasília
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: FapUNIFESP (SciELO)
Date: 10-2016
Abstract: ABSTRACT The aim of this study was to evaluate the effect of different agricultural products on quantitative aspects of carcass, body constituents, cooking loss, shear force and colorimetry of the Longissimus lumborum and Triceps brachii muscles in Santa Ines lambs. 24 Santa Ines female lambs received one of four diets which were isoproteic and isoenergetic with fixed levels of forage (60%) and concentrate (40%) of corn and soybean meal during 45 days. The forages per diet differed: coast-cross hay (HAY), cassava hay (CASS), dehydrated by-product of pea crop (PEA) and sugarcane (SC). The average weight of the lambs at the beginning of the experiment was 26.35kg. Animals were slaughtered in a federally certified abattoir. Initial and final pH, cooking losses, color using the CIELAB system, shear force and the quantity of sarcomeres per 100μm were measured. Hot carcass, cold and half carcass weights were affected by treatments (P .05). The sarcomere length of Triceps brachii muscle 24 hours after slaughter differed between diets and coast-cross hay had the lowest value. The sarcomere length differed significantly between diets and the dehydrated by-product of pea crop had the lowest number of sarcomeres immediately after slaughter compared to other diets. There was no influence of diet on colorimetry, cooking loss and shear force. The decrease in pH followed the development of the process of rigor mortis in the Longissimus lumborum and Triceps brachii muscles in the first hour and up to 24 hours after slaughter. Diets did not alter the pH, water holding capacity, colorimetry or shear force. The pea by-product and sugarcane can replace traditional sources of fodder without depreciation of meat characteristics.
Publisher: FapUNIFESP (SciELO)
Date: 02-2011
DOI: 10.1590/S0102-09352011000100008
Abstract: This work evaluated the effect of freezing on chondrocytes maintained in culture, aiming the establishment of a cell bank for future application as heterologous implant. Chondrocytes extracted from joint cartilage of nine healthy New Zealand White rabbits were cultivated and frozen with the cryoprotector 5% dimethylsulfoxide for six months. Phenotypic and scanning electron microscopy analyses were carried out to identify morphological and functional differences between fresh and thawed cells. After enzymatic digestion, a total of 4.8x10(5)cells per rabbit were obtained. Fresh chondrocytes showed a high mitotic rate and abundant matrix was present up to 60 days of culture. Loss of phenotypic stability was notable in the thawed chondrocytes, with a low labeling of proteoglycans and weak immunostaining of type II collagen. The present study showed important loss of chondrocyte viability under the freezing conditions. For future in vivo studies of heterologous implant, these results suggests that a high number of cells should be implanted in the host site in order to achieve an adequate number of viable cells. Furthermore, the chondrocytes should be implanted after two weeks of culture, when the highest viability rate is found
Publisher: FapUNIFESP (SciELO)
Date: 02-2014
DOI: 10.1590/S0102-09352014000100024
Abstract: Chondrocytes obtained from stifle joint of New Zealand White rabbits were cultivated. Half of cells were maintained in culture for later implantation and the others frozen during six months to evaluate viability. A circular osteochondral defect was created in the right stifle of other twenty seven rabbits. The control group (CG) received no treatment. The thawed (TH) and fresh (FH) heterologous groups received, respectively, an implant of cultivated thawed or fresh heterologous chondrocytes associated with platelet rich plasma (PRP). The CG group showed greatest pain and lameness compared to the other groups seven days after the implantation. Microscopically, at 45 and 90 days, the TH and FH groups showed filling with cartilaginous tissue containing chondrocytes surrounded by a dense matrix of glycosaminoglycans. In the CG group, healing occurred with vascularized fibrous connective tissue without integration to the subchondral bone. Cryopreserved heterologous chondrocytes were viable for implantation and healing of osteochondral lesions the association with PRP allows the fixation of cells in the lesion and offers growth factors which accelerates repair with tissue similar to articular hyaline cartilage.
Publisher: Springer Science and Business Media LLC
Date: 16-11-2020
Publisher: Springer Science and Business Media LLC
Date: 12-08-2010
DOI: 10.1007/S11250-010-9677-0
Abstract: To define the prevalence of anti-Leptospira sp. agglutinins in ewes in the Federal District, Brazil, serum s les from 157 ewes were tested for antibodies against serovars of Leptospira sp. by the microscopic agglutination test. Antibodies were detected in three flocks in a prevalence of 3% (95% CI = 0.4%-5.7%). Considering that sheep and cattle were raised together, the lack of sanitary control could represent a risk to cattle production, which is the most important activity in the Centre-West region of Brazil.
Publisher: FapUNIFESP (SciELO)
Date: 09-2014
DOI: 10.1590/S0100-736X2014000900012
Abstract: O mastocitoma é a neoplasia cutânea maligna de maior frequência em cães. Apesar do estudo e do desenvolvimento de ersos marcadores prognósticos na tentativa de prever o comportamento biológico deste tipo de neoplasia, a graduação histológica continua sendo utilizada como principal delineador prognóstico para os animais acometidos, mesmo não conseguindo prever o comportamento de mastocitomas de diferenciação intermediária. Diferentes marcadores de proliferação e atividade celular vêm sendo testados com resultados promissores, entretanto, em sua maioria, demandam técnicas específicas de coloração e imuno-histoquímica que, ainda hoje, são de alto custo e muitas vezes não estão facilmente disponíveis. O índice mitótico é um método indireto de mensuração da atividade proliferativa celular, que pode ser contabilizado em uma lâmina comum de avaliação histológica e estudos vêm determinando-o como importante marcador prognóstico para o mastocitoma canino. Dada a recente publicação sugerindo nova graduação em dois graus (alto e baixo) e indisponibilidade das técnicas específicas utilizadas na determinação de outros fatores prognósticos, objetivou-se avaliar o poder prognóstico das diferentes classificações, associando-as à sobrevida destes animais e comparando com o índice mitótico. Apesar de isoladamente ambas as classificações histológicas apresentarem associação com a sobrevida (P .001) dos animais acometidos, na análise multifatorial nenhuma foi significativamente associada com a sobrevida (P ,05). O índice mitótico foi o fator prognóstico mais confiável para sobrevida, independente do ponto de corte utilizado (P ,05), principalmente para os mastocitomas grau 2 de Patnaik et al. (1984), que apresentam comportamento biológico variado, sendo também associado à ocorrência de reci as, tanto locais como distantes, e de síndromes paraneoplásicas (P ,05). Conclui-se ser o índice mitótico o fator prognóstico mais significante, na ausência de técnicas específicas mais avançadas. Preconiza-se seu uso associado às graduações histológicas, visando maior poder prognóstico.
Location: Brazil
No related grants have been discovered for Márcio Botelho de Castro.