ORCID Profile
0000-0002-4156-9290
Current Organisation
University of Melbourne
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Publisher: Wiley
Date: 09-2021
DOI: 10.1111/VCP.12933
Publisher: Wiley
Date: 28-10-2014
DOI: 10.1111/VCP.12210
Publisher: Wiley
Date: 30-05-2023
DOI: 10.1111/VCP.13224
Abstract: Enumeration of nucleated red blood cells (NRBCs) in peripheral blood of dogs and cats is performed by manual counting during blood film evaluation. Automated methods have increased precision and accuracy however, most analyzers cannot distinguish leukocytes and NRBCs. The Sysmex XN‐V Series may distinguish NRBCs and leukocytes however, analytical errors occur. We aimed to investigate cases with discrepant automated and manual NRBC counts, and to evaluate reasons for analytical errors. Data from s les with increased NRBCs were collected retrospectively and compared with manual counts performed on blood films using Spearman's correlation, Passing‐Bablok agreement analysis, and Bland–Altman comparisons. Precision of the automated method and interobserver agreement of manual counts were evaluated. Cases with discrepant results were investigated. Agreement between the methods was good at ≤1NRBC ×10 9 /L in dogs and cats, and inadequate at ≥1NRBC ×10 9 /L. The automated method demonstrated a negative proportional difference to the manual method. Precision was good for the automated method (overall CV 7.1%) and interobserver agreement for the manual method was poor overall (mean CV 27.3%, range 0%‐106.1%). Inaccuracies in NRBC enumeration by the automated method occurred with high hematocrits, the mergence of the cell fragments and leukocyte clouds, and the presence of earlier erythroid precursors. NRBC enumeration by the WNR channel on the Sysmex XN‐1000 V is precise and has good agreement with manual counts in canine and feline blood s les at ≤1NRBC ×10 9 /L. Manual film review is indicated for s les with ≥1NRBC ×10 9 /L, earlier erythroid precursors, s les from greyhounds and dehydrated patients, and if gating errors are noted.
Publisher: Wiley
Date: 29-04-2021
DOI: 10.1111/VCP.12948
Abstract: This is the first reported case of hypoglobulinemia in a dog with disseminated plasma cell neoplasia. A 6‐year‐old male intact Rottweiler was referred to the U‐Vet Animal Hospital (Werribee, Vic, Australia) for weight loss, hyporexia, lethargy, vomiting, and soft stools. Examination of a buffy coat preparation and splenic and liver aspirates revealed a monomorphic population of plasmacytoid cells, and the same cells comprised approximately 90% of bone marrow s les submitted for cytologic and histologic evaluation. Biochemistry revealed a hypoglobulinemia, and the presence of an M‐protein was not supported by serum and urine protein electrophoresis or serum immunofixation. Immunohistochemistry demonstrated strong nuclear labeling for MUM‐1.
Location: Canada
No related grants have been discovered for Marja Astrid Oscos Snowball.