ORCID Profile
0000-0001-6087-2730
Current Organisations
Universidad Nacional de Educación a Distancia
,
Instituto de Toxicología de la Defensa.
,
IESE Bussiness Scholl
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Related Links
Publications
Effects of nicotinic acetylcholine receptor-activating alkaloids on anxiety-like behavior in zebrafish
Publisher: Springer Science and Business Media LLC
Date: 15-07-2021
DOI: 10.1007/S11418-021-01544-8
Abstract: Alkaloids are a structurally complex group of natural products that have a erse range of biological activities and significant therapeutic applications. In this study, we examined the acute, anxiolytic-like effects of nicotinic acetylcholine receptor (nAChR)-activating alkaloids with reported neuropharmacological effects but whose effects on anxiety are less well understood. Because α4β2 nAChRs can regulate anxiety, we first demonstrated the functional activities of alkaloids on these receptors in vitro. Their effects on anxiety-like behavior in zebrafish were then examined using the zebrafish novel tank test (NTT). The NTT is a relatively high-throughput behavioral paradigm that takes advantage of the natural tendency of fish to e down when stressed or anxious. We report for the first time that cotinine, anatabine, and methylanatabine may suppress this anxiety-driven zebrafish behavior after a single 20-min treatment. Effective concentrations of these alkaloids were well above the concentrations naturally found in plants and the concentrations needed to induce anxiolytic-like effect by nicotine. These alkaloids showed good receptor interactions at the α4β2 nAChR agonist site as demonstrated by in vitro binding and in silico docking model, although somewhat weaker than that for nicotine. Minimal or no significant effect of other compounds may have been due to low bioavailability of these compounds in the brain, which is supported by the in silico prediction of blood–brain barrier permeability. Taken together, our findings indicate that nicotine, although not risk-free, is the most potent anxiolytic-like alkaloid tested in this study, and other natural alkaloids may regulate anxiety as well.
Effects of Natural Monoamine Oxidase Inhibitors on Anxiety-Like Behavior in Zebrafish
Publisher: Frontiers Media SA
Date: 13-05-2021
DOI: 10.3389/FPHAR.2021.669370
Abstract: Monoamine oxidases (MAO) are a valuable class of mitochondrial enzymes with a critical role in neuromodulation. In this study, we investigated the effect of natural MAO inhibitors on novel environment-induced anxiety by using the zebrafish novel tank test (NTT). Because zebrafish spend more time at the bottom of the tank when they are anxious, anxiolytic compounds increase the time zebrafish spend at the top of the tank and vice versa. Using this paradigm, we found that harmane, norharmane, and 1,2,3,4-tetrahydroisoquinoline (TIQ) induce anxiolytic-like effects in zebrafish, causing them to spend more time at the top of the test tank and less time at the bottom. 2,3,6-trimethyl-1,4-naphtoquinone (TMN) induced an interesting mix of both anxiolytic- and anxiogenic-like effects during the first and second halves of the test, respectively. TIQ was unique in having no observable effect on general movement. Similarly, a reference MAO inhibitor clorgyline—but not pargyline—increased the time spent at the top in a concentration-dependent manner. We also demonstrated that the brain bioavailability of these compounds are high based on the ex vivo bioavailability assay and in silico prediction models, which support the notion that the observed effects on anxiety-like behavior in zebrafish were most likely due to the direct effect of these compounds in the brain. This study is the first investigation to demonstrate the anxiolytic-like effects of MAO inhibitors on novel environment-induced anxiety in zebrafish.
Simultaneous analysis of neutral and acidic pharmaceuticals as well as related compounds by gas chromatography–tandem mass spectrometry in wastewater
Publisher: Elsevier BV
Date: 09-2007
DOI: 10.1016/J.TALANTA.2007.03.053
Abstract: This work presents a new multi-residue analytical method based on solid phase extraction (SPE) with Oasis HLB sorbent, followed by gas chromatography tandem mass spectrometry (GC-MS/MS) for the simultaneous determination of a group of 10 acidic and neutral pharmaceuticals and related compounds in wastewaters. The typical derivation step was avoided, allowing the determination of acidic and neutral pollutants in a single analysis as well as providing a fast and easy method suitable for routine monitoring. Target pollutants include: anti-inflammatory drugs (ibuprofen, acetaminophen and diclofenac) an antiepileptic agent (carbamazepine) stimulants (caffeine and nicotine) an antiseptic (triclosan) a plasticizer (bisphenol A) and two of their more relevant metabolites (2,8-dichlorodibenzo-p-dioxin and 1,7-dimethylxanthine). Recoveries between 66 and 112% were achieved for all the target compounds (except for 2,8-dichlorodibenzo-p-dioxin). Good linearity was observed within the studied ranges (R(2)>0.993). Acceptable intra and inter-day precision was obtained, with relative standard deviation between 2 and 18%. The application of the optimized MS/MS mode allowed method detection limits in the range of 0.2-16ng/L, with the exception of ibuprofen (120ng/L). Finally, the methodology was successfully applied to the analysis of hospital effluent s les. All target analytes were detected at concentrations between 1ng/L and 83215mug/L. Even in the absence of derivatization, all the analytes showed good peak shape, except acetaminophen, which exhibited peak tailing. However, the method proved to be repetitive and reproducible, and the peak shape did not represent a problem for the reliable quantification of this compound. For most of the analytes studied, the detection limits achieved compare well against values reported in previously published methods.
Related Organisations
Universidad Nacional De Educación A Distancia
Location: No location found
Related Funding Activities
No related grants have been discovered for Jorge Hurtado de Mendoza.