ORCID Profile
0000-0002-5011-9611
Current Organisations
Universiti Putra Malaysia
,
Islamic Azad University Mashhad Branch
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Publisher: MDPI AG
Date: 10-12-2022
DOI: 10.3390/ANI12243492
Abstract: Nasturtium officinale contains high amounts of phytochemical compounds that work against oxidative damages leading to improved health conditions in animals as well as humans. The study was performed to investigate the health benefits of nonencapsulated and nanoliposome-encapsulated phenolic rich fractions obtained from Nasturtium officinale on mice induced colorectal cancer. The experiment focused on encapsulation efficiency in improving the effectiveness of plant bioactive compounds. Phenolic rich fractions (PRF) were successfully loaded in the nanoliposome structure, a nanometer in size, of spherical shape and with homogeneous dispersion. Induction of colorectal cancer in mice impaired weight gain and feed intake, liver function and structural characteristics of ileum, while the dietary administration of nanoliposome-encapsulated PRF regulated the expression of Caspase 3, Bax, Bcl2, iNOS and SOD genes in the tumor tissue. The addition of nonencapsulated PRF and nanoliposome encapsulated PRF at the concentration of 100 mg TPC/kg BW/day improved the genes expression, although the nanoliposome-encapsulated PRF revealed better health outcomes compared to nonencapsulated PRF. Furthermore, both PRF improved intestinal morphology when the mice were challenged with colorectal cancer. The higher health promoting activity of nanoliposome-encapsulated PRF could be associated with its enhanced intestinal absorption, bioavailability, bioaccessibility and bioactivity. Consequently, the nanoliposome-encapsulated PRF could be considered as a promising anticancer agent against colorectal cancer.
Publisher: MDPI AG
Date: 30-01-2012
Publisher: MDPI AG
Date: 29-11-2011
DOI: 10.3390/IJMS12128610
Publisher: MDPI AG
Date: 07-10-2023
DOI: 10.3390/IJTM3040029
Publisher: MDPI AG
Date: 06-09-2010
Publisher: Termedia Sp. z.o.o.
Date: 09-07-2022
DOI: 10.5114/AOMS/152000
Abstract: We aimed aimed to assess the effect of the immunotherapy with the PCSK9 peptide vaccine on the hepatic expression levels of microRNAs associated with LDLR pathway including miRNA-27a, miRNA-30c, and miRNA-191 in normal vaccinated mice. PCSK9 immunogenic peptide and 0.4% alum adjuvant were mixed at a 1:1 ratio and used as a vaccine formulation. Male albino mice were randomly assigned into vaccine or control groups. Mice in the vaccine group were injected four times at two-week intervals with a PCSK9 peptide vaccine, and mice in the control group were injected with phosphate-buffered saline. Animal livers were s led two weeks after the last injection to assess miRNAs expression levels. The hepatic expression level of miRNA-27a, miRNA-30c, and miRNA-191 were evaluated by SYBR Green Real-Time PCR, quantified by comparative (2-ΔΔCT) method (Fold change) and normalized to U6 small nuclear RNA expression as an internal control. The hepatic expression level of miRNA-27a showed significant reduction in mice following immunotherapy with the PCSK9 peptide vaccine when compared to the control group (FC:0.731±0.1, P=0.027). Also, there was a borderline significant reduction in the hepatic expression level of miRNA-30c in the vaccinated group compared to the control (FC: 0.569±0.1, P=0.078). However, no significant differences were found in the hepatic expression level of miRNA-191 between two studied groups (FC: 0.852±0.1, P=0.343). According to the findings, the PCSK9 peptide vaccine could effectively reduce the hepatic expression level of miRNA-27a and may be helpful in the management of LDL-C level and atherosclerosis, which may be mediated through LDLR pathway.
Publisher: MDPI AG
Date: 25-04-2022
Abstract: Essential oils are important compounds for the prevention and/or treatment of various diseases in which solubility and bio-accessibility can be improved by nanoemulsion systems. Heracleum persicum oil nanoemulsion (HAE-NE) was prepared and biological properties were investigated against human breast cancer cells and normal human fibroblasts foreskin. Particle size, zeta potential and poly dispersity index were 153 nm, −47.9 mV and 0.35, respectively. (E)anethole (57.9%), terpinolene (13.8%), ɣ-terpinene (8.1%), myrcene (6.8%), hexyl butyrate (5.2%), octyl butanoate (4.5%) and octyl acetate (3.7%) was detected in nanoemulsion. Proliferation of cancer cells at IC50 = 2.32 μg/mL was significantly (p 0.05) inhibited, and cell migration occurred at 1.5 μL/mL. The HAE-NE at 1.5, 2.5 and 3.5 µg/concentration up-regulated caspase 3 and enhanced sub-G1 peak of cell cycle with nil cytotoxic effects in the liver, kidney and jejunum of mice. Villus height, villus width, crypt depth and goblet cells in mice group fed with 10 and 20 mg/kg body weight of HAE-NE improved. Cellular redox state in the liver indicated 10 and 20 mg/kg body weight of nanoemulsion significantly up-regulated the expression of SOD, CAT and GPx genes. Heracleum persicum oil nanoemulsion could be an eco-friendly nanotherapeutic option for pharmaceutical, cosmetological and food applications.
Publisher: Springer Science and Business Media LLC
Date: 15-08-2021
Location: Iran (Islamic Republic of)
No related grants have been discovered for Ehsan Karimi.