ORCID Profile
0000-0001-7291-5080
Current Organisations
University Putra Malaysia
,
University of Riau
,
Universiti Putra Malaysia
,
Universitas Andalas
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Publications
Antibacterial properties of Pyrrosia longifolia extracts
Publisher: International Pharmaceutical Federation (FIP)
Date: 15-05-2023
DOI: 10.46542/PE.2023.232.168173
Abstract: Background: Pyrrosia longifolia is an epiphytic plant found in tropical forests. This plant is known to have antioxidant, anti-diabetic, anti-inflammatory, and antibacterial properties and is widely used in traditional medicine. Pyrrosia longifolia is one of the species used in traditional medicine, but no evidence of antibacterial activity has been found. Objective: The purpose of this study is to determine the antibacterial activity of various P. longifolia extracts. Methods: The plant was macerated with methanol and separated based on its polarity. The well diffusion method was used to determine the plant’s antibacterial activity through the inhibition zones - minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of bacterial growth against seven bacteria pathogens. Results: The antibacterial activity of the extracts showed various inhibitions of the bacteria tested. Dichloromethane and ethyl acetate extracts showed intermediate inhibitory activity on Bacillus subtilis ATCC 19659, B. cereus ATCC 10876, Salmonella typhimurium ATCC 142028, and Vibrio parahaemolyticus ATCC 17802. Conclusion: The extract of P. longifolia is susceptible to the test bacteria, and additional testing is necessary to determine its antibacterial activity.
Antibacterial in vitro screening of Helminthostachys zeylanica (L.) Hook. root extracts
Publisher: Garval Editorial Ltda.
Date: 03-2023
DOI: 10.56499/JPPRES22.1540_11.2.291
Abstract: Context: Helminthostachys zeylanica (L.) Hook. has been used as a traditional medicine plant to treat diabetic mellitus and various infectious in China, Malaysia, and Indonesia. Aims: To determine the antibacterial activity of H. zeylanica root extracts against various pathogenic bacteria. Methods: The extraction was carried out by maceration of fresh roots using methanol, then partitioned to obtain extracts of n-hexane, dichloromethane, ethyl acetate, and water. The extracts were tested for antibacterial activity using the agar diffusion method, minimal inhibition concentration (MIC), and minimal bactericidal concentration (MBC). Results: Dichloromethane and ethyl acetate extracts showed inhibitory activity against Bacillus subtilis ATCC 1965, Staphylococcus aureus ATCC 6538, B. cereus ATCC 10876, Vibrio parahaemolyticus ATCC 17802, V. alginolyticus ATCC 17749, Listeria monocytogenes ATCC 7644, and Salmonella typhimurium ATCC 142028. The MIC value of dichloromethane extract against these bacteria was 125-500 µg/mL, while the ethyl acetate extract was 500 µg/mL. Furthermore, the MBC for dichloromethane against bacteria B. subtilis and V. parahaemolyticus were 250 µg/mL. Conclusions: The study demonstrated the in vitro antibacterial potential of the species extracts against pathogenic bacteria. The dichloromethane and ethyl acetate extracts showed the most significant inhibitory activity, suggesting that this plant could be a potential source for developing new antimicrobial agents. Further research is needed to identify the active compounds responsible for the observed effects.
Characterization, molecular identification, and antimicrobial activity of lactic acid bacteria isolated from selected fermented foods and beverages in Malaysia
Publisher: Oxford University Press (OUP)
Date: 2023
Abstract: The present work investigated the profile and bio ersity of lactic acid bacteria (LAB) isolated from selected manufactured and homemade fermented foods in Malaysia. A total of 55 LAB were isolated from 20 s les, and identified based on the sequencing of 16S rRNA gene. The LAB isolates were identified as Lacticaseibacillus rhamnosus (34.5%), Lactiplantibacillus plantarum (20%), Limosilactobacillus fermentum (20%), Lacticaseibacillus paracasei (12.7%), Lacticaseibacillus casei (3.6%), Lactobacillus sp. (1.8%), Enterococcus faecalis (3.6%), Enterococcus faecium (1.8%), and Enterococcus durans (1.8%). Majority (94%) of the LAB isolates exhibited broad-spectrum antimicrobial activity against selected foodborne pathogens, and four isolates (L. fermentum SC1001, L. paracasei K2003, and L. rhamnosus KF1002 and MK2003) could produce bacteriocin-like inhibitory substance (BLIS). Lacticaseibacillus paracasei M1001 (homemade mozzarella) exhibited high-temperature tolerance and acid resistance, was homofermentative, and generated good antimicrobial activity, which strongly implied its potential for industrial applications. The present work results would potentially widen our knowledge of LAB ersity in Malaysian fermented foods and provide a potential for their applications in the food industry or other purposes.
Free Radical Scavenging, Anti-Infectious, and Toxicity Activities from Stenochlaena palustris (Burm.f.) Bedd. Extracts
Publisher: Hindawi Limited
Date: 11-2022
DOI: 10.1155/2022/5729217
Abstract: Stenochlaena palustris (Burm.f.) Bedd. (Blechnaceae) or Kelakai is a traditional medicinal plant found in the Indonesian islands of Borneo and Sumatra. It has been used to treat wound healing, infection, and diabetes. This study looked into the free radical scavenging activity, antiplasmodial activity, toxicity, and antibacterial activity against pathogenic bacteria. The species’ aerial part was extracted with methanol, followed by a liquid-liquid extraction against (n-hexane, dichloromethane, and ethyl acetate). The extracts’ free radical scavenging activities were determined using DPPH and NO radicals. The antiplasmodial and toxicity assays were conducted using two Plasmodium falciparum strains (3D7 and W2) and the brine shrimp lethality test. In addition, antibacterial activity was determined using the well diffusion method. The results revealed that ethyl acetate depicted potential activities toward the assay. The ethyl acetate showed potential free radical scavenging activities with an IC50 value of 51.63 ± 0.46 μg/mL (DPPH) and 60.03 ± 0.65 μg/mL (NO). The antiplasmodial activities showed that the ethyl acetate had potential activities among the extracts with an IC50 value of 11.06 ± 0.45 μg/mL. However, all the extracts demonstrated nontoxic toward Artemia salina with LC50 1000 μg/mL. Furthermore, the ethyl acetate demonstrated intermediate susceptibility against B. cereus ATCC 10876, V. parahaemolyticus ATCC 17802, L. monocytogenes ATCC 7644, and S. Typhimurium ATCC 14028 at a concentration of 500 μg/disc. According to these findings, the ethyl acetate extract of S. palustris (Burm.f.) Bedd is a promising source of natural antioxidants and antiplasmodial agents.
Green synthesis of silver nanoparticles from Alpinia galanga extract with microwave irradiation and antibacterial activity against Escherichia coli
Publisher: International Pharmaceutical Federation (FIP)
Date: 31-03-2022
DOI: 10.46542/PE.2022.222.2023
Abstract: Background: This study deals with the rapid green synthesis of silver nanoparticles (Ag NPs) by microwave irradiation using water extract of Alpinia galanga as a reducing agent. Methods: The effect of concentrations of water extract and silver nitrate aqueous solution (1 mM) on the synthesis was investigated using the ratios 1:1, 1:5, 1:10, and 1:20. The formation of Ag NPs was observed after being microwaved for 30, 60, 90, 120, 150, and 180 seconds. Results: The best Ag NPs were produced from the ratio of 1:10 after 180 s irradiation, confirmed by the optimum absorbance at 450 nm using a UV-visible spectrophotometer. Transmission electron microscopy analysis showed that the size of the Ag NPs was 50 nm. Their antibacterial activity was determined using Resazurin microtiter assay against Escherichia coli, and the results showed that it inhibited 94.4% of E. coli. It was significantly different (p 0.05) with the inhibition of extract and Amoxsan, which inhibited 71.1% and 85.8% of E. coli, respectively. Conclusion: The use of microwave irradiation on the synthesis of silver nanoparticles using Alpinia galanga extract as a reducing agent appears to be rapid and eco-friendly.
High prevalence of multiple antibiotic resistance in fermented food-associated lactic acid bacteria in Malaysia
Publisher: Elsevier BV
Date: 05-2023
Antibacterial Activity of Red Dragon Peel (Hylocereus polyrhizus) Pigment
Publisher: IOP Publishing
Date: 11-2019
DOI: 10.1088/1742-6596/1351/1/012042
Abstract: Red dragon fruit ( Hylocereus polyrhizus ) is a species of plant that provides natural pigment which is betalain. Betalain is a one of the pigments that gives a natural colour to flowers and fruits. Moreover, of betalain in red dragon fruit peel, there are bioactive compound such as polyphenol and flavanoid with antioxidant and antibacteria activities. This research was objected to extract pigments and others secondary metabolites from red dragon fruit peel using ethanol, methanol, dicloromethane and ethyl acetate solvents, continued with determined potential antibacteria activity using Resazurin Microtitter Assay (REMA) method. The results of antibacterial activity showed inhibition percentage against test of positive Gram bacteria Streptococcus aureus ATCC 29213 with extract of n -haxane, ethyl acetate and pigment sequentially were 67.5, 67.0, 85.1, 83.1 % and Bacillus subitilis ATCC 11774 were 73.4, 98.6, 97.1, 100 % and negative Gram bacteria Escherichia coli ATCC 35218 were 70.0, 73.1, 74.2, 94. 9 % and Vibrio algynolyticus were 72.6, 73.1, 74.2, 94.9 % at final concentration of 20 μg/mL
Cytotoxicity and Antiplasmodial Properties of Different Hylocereus polyrhizus Peel Extracts
Publisher: International Scientific Information, Inc.
Date: 26-05-2021
Red dragon peel (Hylocereus polyrhizus) as antioxidant source
Publisher: AIP Publishing
Date: 2020
DOI: 10.1063/5.0001391
Related Organisations
Related Funding Activities
No related grants have been discovered for Yuli Haryani.