ORCID Profile
0000-0003-1511-816X
Current Organisations
University of Natural Sources and Life Sciences
,
National Institute for Biotechnology and Genetic Engineering
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Publisher: MDPI AG
Date: 13-11-2022
DOI: 10.3390/SU142214997
Abstract: Antibiotic contamination of water is an emerging global issue with severe implications for both public health and the environment. Ciprofloxacin (CIP) is a synthetic fluoroquinolone antibiotic, which is broadly used in human and veterinary medicines around the world to treat various bacterial infections. The presence of CIP in the aquatic environment poses serious health problems to human beings and other living entities. Floating treatment wetland (FTW) is a low-cost and eco-friendly wastewater remediation technology. In the current study, the Canna indica. (Indian shot) was vegetated in a floatable mat to develop FTWs. A consortium of three bacterial strains, Acinetobacter lwoffii ACRH76, Bacillus pumulis C2A1, and Acinetobacter sp. HN3, was immobilized on iron oxide nanoparticles (Fe3O4-NPs) and augmented in the FTWs for the remediation of CIP-contaminated (100 mg/L) water. The augmentation of bacteria (immobilized or free) in the FTWs significantly enhanced the removal of CIP from water. The maximum reduction in CIP (98%), chemical oxygen demand (COD 90%), biochemical oxygen demand (BOD 93%) and total organic carbon (TOC 95%) was observed in FTWs that had Fe3O4-NP supported bacteria. This study reveals that FTWs have a great potential to remove the CIP from contaminated water, albeit its CIP removal efficiency was substantially enhanced by augmentation with Fe3O4-NPs supported bacteria.
Publisher: MDPI AG
Date: 18-01-2023
DOI: 10.3390/W15030391
Abstract: Chromium (Cr)-contaminated tannery wastewater is a major environmental concern, especially in developing countries, such as Pakistan, due to its use for crop irrigation, resulting in food-chain contamination and health issues. In this study, we explored the distribution, speciation, hydrogeochemical behavior and environmental risks of Cr in tannery wastewater collected from various tanneries of Kasur district in Punjab, Pakistan. Tannery wastewater s les were taken during the summer (TWW-summer n = 82) and winter (TWW-winter n = 82) seasons. The results showed that high Cr concentration was observed in TWW-winter (mean: 49 ± 32 mg L−1) compared to TWW-summer (mean: 15 ± 21 mg L−1). In TWW-summer and TWW-winter s les, the Cr concentration exceeded the National Environmental Quality Standard (1 mg L−1), with the total Cr ranging from 2.8 to 125 mg L−1. Hexavalent Cr (Cr(VI)) and trivalent Cr (Cr(III)) concentrations spanned 2.7 to 2.9 and 12.4 to 46 mg L−1, respectively. The Piper plot showed that hydrogeochemistry of wastewater was dominated by Ca-Mg-SO4 and Ca-Mg-Cl type water, and geochemical modeling indicated that the presence of Cr-iron (Fe)-bearing mineral phases—notably, FeCr2O4, MgCr2O4 and Cr(OH)3) may control the fate of Cr in the tannery wastewater. Environmental risk assessment modeling categorized the tannery wastewater as the ‘worst quality’, which is not fit for use in crop irrigation without treatment. This study highlights that immediate monitoring, remediation and mitigation strategies are required to reduce the risk of Cr exposure from tannery wastewater in many areas of Pakistan.
Publisher: MDPI AG
Date: 26-04-2022
DOI: 10.3390/SU14095230
Abstract: Water scarcity is one of the key global challenges affecting food safety, food security, and human health. Constructed wetlands (CWs) provide a sustainable tool to remediate wastewater. Here we explored the potential of vertical flow-CWs (VF-CWs) vegetated with ten indigenous wetland plant species to treat chromium (Cr)-contaminated water. The wetland plants were vegetated to develop VF-CWs to treat Cr-contaminated water in a batch mode. Results revealed that the Cr removal potential of VF-CWs vegetated with different wetland plants ranged from 47% to 92% at low (15 mg L−1) Cr levels and 36% to 92% at high (30 mg L−1) Cr levels, with the maximum (92%) Cr removal exhibited by VF-CWs vegetated with Leptochloa fusca. Hexavalent Cr (Cr(VI)) was reduced to trivalent Cr (Cr(III)) in treated water (96–99 %) of all VF-CWs. All the wetland plants accumulated Cr in the shoot (1.9–34 mg kg−1 dry weight (DW)), although Cr content was higher in the roots (74–698 mg kg−1 DW) than in the shoots. Brachiaria mutica showed the highest Cr accumulation in the roots and shoots (698 and 45 mg kg−1 DW, respectively), followed by Leptochloa fusca. The high Cr level significantly (p 0.05) decreased the stress tolerance index (STI) percentage of the plant species. Our data provide strong evidence to support the application of VF-CWs vegetated with different indigenous wetland plants as a sustainable Cr-contaminated water treatment technology such as tannery wastewater.
Publisher: Springer Science and Business Media LLC
Date: 17-01-2018
DOI: 10.1007/S10529-018-2510-Y
Abstract: As PCR methods have improved over the last 15 years, there has been an upsurge in the number of new DNA marker tools, which has allowed the generation of high-density molecular maps for all the key Brassica crop types. Biotechnology and molecular plant breeding have emerged as a significant tool for molecular understanding that led to a significant crop improvement in the Brassica napus species. Brassica napus possess a very complicated polyploidy-based genomics. The quantitative trait locus (QTL) is not sufficient to develop effective markers for trait introgression. In the coming years, the molecular marker techniques will be more effective to determine the whole genome impairing desired traits. Available genetic markers using the single-nucleotide sequence (SNP) technique and high-throughput sequencing are effective in determining the maps and genome polymorphisms amongst candidate genes and allele interactions. High-throughput sequencing and gene mapping techniques are involved in discovering new alleles and gene pairs, serving as a bridge between the gene map and genome evaluation. The decreasing cost for DNA sequencing will help in discovering full genome sequences with less resources and time. This review describes (1) the current use of integrated approaches, such as molecular marker technologies, to determine genome arrangements and interspecific outcomes combined with cost-effective genomes to increase the efficiency in prognostic breeding efforts. (2) It also focused on functional genomics, proteomics and field-based breeding practices to achieve insight into the genetics underlying both simple and complex traits in canola.
Publisher: Elsevier BV
Date: 05-2019
DOI: 10.1016/J.JENVMAN.2019.02.082
Abstract: Environmental variations resulting in biotic and abiotic stresses demand adaptive changes in the photosynthetic machinery. To cope with these challenges, plant scientists are constantly striving to enhance photosynthetic activity. The photorespiration pathway, which fixes O
Publisher: Informa UK Limited
Date: 27-07-2023
Location: Pakistan
No related grants have been discovered for Muhammad Afzal.