Manali Mukherjee

Lasting changes to circulating leukocytes in people with mild SARS-CoV-2 infections

Publisher: Cold Spring Harbor Laboratory

Date: 27-09-2021

DOI: 10.1101/2021.09.25.21264117

Abstract: Survivors of severe SARS-CoV-2 infections frequently suffer from a range of post-infection sequelae. Whether survivors of mild or asymptomatic infections can expect any long-term health consequences is not yet known. Herein we investigated lasting changes to soluble inflammatory factors and cellular immune phenotype and function in in iduals who had recovered from mild SARS-CoV-2 infections (n=22) compared to those that had recovered from other mild respiratory infections (n=11). In iduals who had mild SARS-CoV-2 infections had elevated levels of C-reactive protein 1-3 months after symptom onset, and changes in phenotype and function of circulating T cells that were not apparent in in iduals 6-9 months post-symptom onset. Markers of monocyte activation and expression of adherence and chemokine receptors indicative of altered migratory capacity were also higher at 1-3 months post-infection in in iduals who had mild SARS-CoV-2, but these were no longer elevated by 6-9 months post-infection. Perhaps most surprisingly, polyclonal activation of T cells was higher in in iduals who had recently experienced a mild SARS-CoV-2 infection compared to in iduals with other recent respiratory infections. These data are indicative of prolonged immune activation and systemic inflammation that persists for up to three months after mild or asymptomatic SARS-CoV-2 infections.

Detecting immunoglobulins in processed sputa

Publisher: Wiley

Date: 21-08-2021

DOI: 10.1111/ALL.15049

Impact of former smoking exposure on airway eosinophilic activation and autoimmunity in patients with severe asthma

Publisher: European Respiratory Society (ERS)

Date: 02-03-2022

DOI: 10.1183/13993003.02446-2021

Abstract: Severe eosinophilic asthma is characterised by frequent exacerbations and a relative insensitivity to steroids. Experimentally, smoking may induce eosinophilic airway inflammation, but the impact in patients with severe asthma is not clear. To investigate the association between smoking exposure in patients with severe asthma, and eosinophilic inflammation and activation, as well as airway autoimmunity and steroid responsiveness. Patients with severe asthma according to European Respiratory Society/American Thoracic Society criteria were assessed with sputum s les, analysed by cell differential count, and for the presence of free eosinophil granules (FEGs), autoantibodies against eosinophil peroxidase (EPX) and macrophage receptor with collagenous structure (MARCO). A subgroup of patients with eosinophilic airway inflammation was re-assessed after a 2-week course of prednisolone. 132 severe asthmatics were included in the study. 39 (29.5%) patients had ≥10 pack-years of smoking history: 36 (27.3%) were former smokers and three (2.3%) current smokers and 93 (70.5%) had pack-years exposure. Eosinophilic airway inflammation was more prevalent among patients with ≥10 pack-years (66.7%), compared to patients with pack-years (38.7%, p=0.03), as was the level of FEGs (p=0.001) and both anti-EPX and anti-MARCO (p .05 and p .0001, respectively). Omitting current smokers did not affect these associations. Furthermore, prednisolone reduced, but did not normalise, sputum eosinophils in patients with a ≥10 pack-year smoking history. In patients with severe asthma, a former smoking history is associated with eosinophilic airway inflammation and activation and relative insensitivity to steroids, as well as airway autoimmunity.

Centralized iron-dosing into returned sludge brings multifaceted benefits to wastewater management

Publisher: Elsevier BV

Date: 09-2021

DOI: 10.1016/J.WATRES.2021.117536

McMaster University

Location: Canada

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University Of Queensland

Location: Australia

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University Of Pune

Location: India

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University Of Queensland, Australia

Location: Australia

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University Of Nottingham

Location: United Kingdom of Great Britain and Northern Ireland

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University Of Abertay Dundee

Location: United Kingdom of Great Britain and Northern Ireland

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National University Of Singapore

Location: Singapore

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Lee Kong Chian School Of Medicine

Location: Singapore

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Discovery Early Career Researcher Award - Grant ID: DE150100393

Start Date: 02-2015

End Date: 12-2018

Amount: $335,000.00

Funder: Australian Research Council

Linkage Projects - Grant ID: LP190100329

Start Date: 12-2020

End Date: 12-2024

Amount: $388,400.00

Funder: Australian Research Council

Discovery Projects - Grant ID: DP120102832

Start Date: 05-2012

End Date: 12-2015

Amount: $390,000.00

Funder: Australian Research Council

Linkage Projects - Grant ID: LP130100361

Start Date: 02-2014

End Date: 02-2017

Amount: $325,000.00

Funder: Australian Research Council

Discovery Projects - Grant ID: DP180103369

Start Date: 06-2018

End Date: 06-2024

Amount: $277,614.00

Funder: Australian Research Council

Industrial Transformation Research Hubs - Grant ID: IH210100001

Start Date: 07-2022

End Date: 07-2026

Amount: $2,062,428.00

Funder: Australian Research Council