ORCID Profile
0000-0002-0797-2502
Current Organisation
Universidade Federal de Lavras
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Publisher: Research Square Platform LLC
Date: 03-03-2021
DOI: 10.21203/RS.3.RS-282328/V1
Abstract: Fungi Induratia have been poorly evaluated for their non-volatile secondary metabolites. In the present work, we evaluated the effects of non-volatile secondary metabolites released into the culture medium by Induratia spp. upon toxic alterations induced by Bothrops spp. venoms. B. atrox venom phospholipase was inhibited by Induratia sp. and I. yucatanensis around 12 and 16%. The extracts of two strains inhibited 12 to 25% of the hemolysis induced by B.moojeni venom. Thrombolysis was inhibited by 30 to 60% by the compounds present in two extracts. The coagulation induced by B. moojeni venom was prolonged by 26 to 41 s by the action of the extracts of I. yucatanensis and I. coffeana . The fungal extracts did not exerted any cytotoxic effect, nor did they induce any alteration in the other evaluated substrates show the potential use of non-volatile metabolites produced by the fungi evaluated as enzyme modulators, especially for proteases with a fundamental role in human hemostasis.
Publisher: Microbiology Society
Date: 2016
DOI: 10.1099/MIC.0.000168
Abstract: Production of pigments by filamentous fungi is gaining interest owing to their use as food colourants, in cosmetics and textiles, and because of the important biological activities of these compounds. In this context, the objectives of this study were to select pigment-producing fungi, identify these fungi based on internal transcribed spacer sequences, evaluate the growth and pigment production of the selected strains on four different media, and characterize the major coloured metabolites in their extracts. Of the selected fungal strains, eight were identified as Aspergillus sydowii (CML2967), Aspergillus aureolatus (CML2964), Aspergillus keveii (CML2968), Penicillium flavigenum (CML2965), Penicillium chermesinum (CML2966), Epicoccum nigrum (CML2971), Lecanicillium aphanocladii (CML2970) and Fusarium sp. (CML2969). Fungal pigment production was influenced by medium composition. Complex media, such as potato dextrose and malt extract, favoured increased pigment production. The coloured compounds oosporein, orevactaene and dihydrotrichodimerol were identified in extracts of L. aphanocladii (CML2970), E. nigrum (CML2971), and P. flavigenum (CML2965), respectively. These results indicate that the selected fungal strains can serve as novel sources of pigments that have important industrial applications.
Publisher: Oxford University Press (OUP)
Date: 08-2019
DOI: 10.1111/LAM.13171
Abstract: Natural enzyme inhibitors have been widely described in literature because of its pharmacological and cosmetic applications. Fungi found in caves represent a promising source of bioactive substances that are still little explored scientifically. Thus, the present work evaluated the presence of enzymatic modulators in a filtrate obtained from the cultivation of the cave fungus Lecanicillium aphanocladii (Family: Cordycipitaceae). Snake venoms from Bothrops alternatus and Bothrops atrox were used as an enzymatic source for the induction of the phospholipase, proteolytic, thrombolytic, cytotoxic and coagulant activities. Compounds present in the fungal filtrate inhibited 50, 23·8, 26·6, 50·9 and 52·5% of the proteolytic, phospholipase, haemolytic, thrombolytic and coagulant activities respectively. The filtrate was not cytotoxic on erythrocytes, but induced partial dissolution of thrombi. Fungal enzyme inhibitors that have low or no toxicity and can be obtained on a large scale and at low cost are relevant in the medical-scientific context. Therefore, the inhibition of phospholipases A2 and proteases observed in the present work highlights the potential of fungal metabolites for the development of drugs that can be used in the treatment of haemostasis and inflammation-related disorders. In this study, secondary metabolites synthesized by Lecanicillium aphanocladii, a fungus isolated from caves, demonstrated modulating action on proteases and phospholipases A2 present in snake venoms of the Bothrops genus, widely used as tools for the study of pathophysiology processes related to haemostasis and inflammation. The results suggest the possibility of future applications for these metabolites in the development of pharmaceuticals of medical-scientific interest.
Publisher: Elsevier BV
Date: 2012
Publisher: MDPI AG
Date: 07-07-2021
DOI: 10.3390/JOF7070541
Abstract: Filamentous fungi are known to biosynthesize an extraordinary range of azaphilones pigments with structural ersity and advantages over vegetal-derived colored natural products such agile and simple cultivation in the lab, acceptance of low-cost substrates, speed yield improvement, and ease of downstream processing. Modern genetic engineering allows industrial production, providing pigments with higher thermostability, water-solubility, and promising bioactivities combined with ecological functions. This review, covering the literature from 2020 onwards, focuses on the state-of-the-art of azaphilone dyes, the global market scenario, new compounds isolated in the period with respective biological activities, and biosynthetic pathways. Furthermore, we discussed the innovations of azaphilone cultivation and extraction techniques, as well as in yield improvement and scale-up. Potential applications in the food, cosmetic, pharmaceutical, and textile industries were also explored.
Publisher: Springer Science and Business Media LLC
Date: 1999
Publisher: Springer Science and Business Media LLC
Date: 12-10-2021
Publisher: Springer Science and Business Media LLC
Date: 23-03-2006
Abstract: Brucella spp . are facultative intracellular pathogens that have the ability to survive and multiply in professional and non-professional phagocytes, and cause abortion in domestic animals and undulant fever in humans. Several species are recognized within the genus Brucella and this classification is mainly based on the difference in pathogenicity and in host preference. Brucella strains may occur as either smooth or rough, expressing smooth LPS (S-LPS) or rough LPS (R-LPS) as major surface antigen. This bacterium possesses an unconventional non-endotoxic lipopolysaccharide that confers resistance to anti-microbial attacks and modulates the host immune response. The strains that are pathogenic for humans ( B. abortus , B. suis , B. melitensis ) carry a smooth LPS involved in the virulence of these bacteria. The LPS O-chain protects the bacteria from cellular cationic peptides, oxygen metabolites and complement-mediated lysis and it is a key molecule for Brucella survival and replication in the host. Here, we review i) Brucella LPS structure ii) Brucella genome, iii) genes involved in LPS biosynthesis iv) the interaction between LPS and innate immunity.
Publisher: Elsevier BV
Date: 06-2010
Publisher: FapUNIFESP (SciELO)
Date: 2020
Publisher: Universidade Estadual de Maringa
Date: 10-03-2015
Publisher: Elsevier BV
Date: 06-2010
DOI: 10.1016/J.IJFOODMICRO.2010.03.029
Abstract: Cauim is a fermented beverages prepared by Tapirapé Amerindians in Brazil from substrates such as cassava, rice, peanuts, pumpkin, cotton seed and maize. Here we study the microorganisms associated with peanut and rice fermentation using a combination of culture-dependent and -independent methods. The bacterial population varied from 7.4 to 8.4 log CFU/ml. The yeast population varied from 4.0 to 6.6 log CFU/ml. A total of 297 bacteria and yeasts strains were isolated during fermentation, with 198 bacteria and 99 yeast. The Lactobacillus genus was dominant throughout fermentation. Bacteria and yeast community dynamics during the fermentation process were monitored by PCR-DGGE analysis. Both culture-dependent and -independent methods indicated that the bacterial species L. plantarum, L. fermentum, L. paracasei and L. brevis as well as the yeast species P. guilliermondii, K. lactis, Candida sp, R. toruloides and Saccharomyces cerevisiae, were dominant during fermentation. Multivariate analysis of microorganisms during beverage fermentation showed that the microbial community changed during the fermentation process.
Publisher: Elsevier BV
Date: 10-2017
DOI: 10.1016/J.VACCINE.2010.06.023
Abstract: Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusDeltawbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-alpha when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. DeltawbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence.
Publisher: FapUNIFESP (SciELO)
Date: 2020
Publisher: Springer Science and Business Media LLC
Date: 21-05-2020
Publisher: Springer Science and Business Media LLC
Date: 06-11-2021
Publisher: Springer Science and Business Media LLC
Date: 03-07-2008
Publisher: Universidade Federal de Minas Gerais - Pro-Reitoria de Pesquisa
Date: 22-12-2022
DOI: 10.35699/2237-5864.2022.39531
Abstract: Neste trabalho, relatamos a experiência de incluir um estudante com deficiência visual nas aulas práticas da disciplina de Microbiologia Geral. Como estratégia, foram elaborados alguns modelos didáticos importantes dentro do conteúdo da disciplina. Para sua elaboração, foram utilizados materiais de fácil aquisição e de baixo custo. O uso dos modelos didáticos nas aulas práticas facilitou o aprendizado do conteúdo das aulas, permitindo a identificação dos detalhes microscópicos pelo estudante com deficiência visual e de todos os outros estudantes que demonstraram entusiasmo e interesse, relatando que os materiais lúdicos adaptados auxiliaram no entendimento de várias estruturas e microrganismos observados na microscopia ótica e nas ilustrações de apostila e livros.
Publisher: Springer Science and Business Media LLC
Date: 09-01-2011
Publisher: FapUNIFESP (SciELO)
Date: 2020
Publisher: FapUNIFESP (SciELO)
Date: 06-2011
DOI: 10.1590/S0100-06832011000300001
Abstract: The process of biological nitrogen fixation (BNF), performed by symbiotic nitrogen fixing bacteria with legume species, commonly known as α and β rhizobia, provides high sustainability for the ecosystems. Its management as a biotechnology is well succeeded for improving crop yields. A remarkable ex le of this success is the inoculation of Brazilian soybeans with Bradyrhizobium strains. Rhizobia produce a wide ersity of chemical structures of exopolysaccharides (EPS). Although the role of EPS is relatively well studied in the process of BNF, their economic and environmental potential is not yet explored. These EPS are mostly species-specific heteropolysaccharides, which can vary according to the composition of sugars, their linkages in a single subunit, the repeating unit size and the degree of polymerization. Studies have showed that the EPS produced by rhizobia play an important role in the invasion process, infection threads formation, bacteroid and nodule development and plant defense response. These EPS also confer protection to these bacteria when exposed to environmental stresses. In general, strains of rhizobia that produce greater amounts of EPS are more tolerant to adverse conditions when compared with strains that produce less. Moreover, it is known that the EPS produced by microorganisms are widely used in various industrial activities. These compounds, also called biopolymers, provide a valid alternative for the commonly used in food industry through the development of products with identical properties or with better rheological characteristics, which can be used for new applications. The microbial EPS are also able to increase the adhesion of soil particles favoring the mechanical stability of aggregates, increasing levels of water retention and air flows in this environment. Due to the importance of EPS, in this review we discuss the role of these compounds in the process of BNF, in the adaptation of rhizobia to environmental stresses and in the process of soil aggregation. The possible applications of these biopolymers in industry are also discussed.
Publisher: Elsevier BV
Date: 08-2010
DOI: 10.1016/J.VETMIC.2010.01.021
Abstract: The goals of this study were to develop a PCR technique to detect ascV and aopB genes from the type III secretion system (T3SS), to evaluate the frequency of these genes in Aeromonas hydrophila strains isolated from diseased fish and from aquaculture environments, and to determine the relationship between the presence of these genes and virulence of A. hydrophila in Nile tilapia. The PCR assay developed here successfully detected the target genes, showing three different profiles for the strains ascV+/aopB+, ascV+/aopB-, and ascV-/aopB-. A higher frequency of ascV+/aopB+ was verified in isolates from diseased fish compared to those from aquaculture environments (P<0.05). Among 64 isolates from diseased fish, ascV+/aopB+ (62.5%) was the most frequent profile (P<0.05) and caused more intensive mortality rates. Environmental strains containing the ascV+/aopB+ profile were less virulent than isolates from clinical cases. These results suggest that the presence of a functional T3SS probably increases the virulence of A. hydrophila. The PCR technique was shown to be a specific and efficient tool for detection of T3SS, and this technique can be used for virulence typing of A. hydrophila isolates.
Publisher: FapUNIFESP (SciELO)
Date: 2013
Publisher: Springer Science and Business Media LLC
Date: 30-01-2022
DOI: 10.1007/S00203-022-02764-6
Abstract: In this study, we evaluated the antibacterial and antioxidant activities of ethyl acetate (EtOAc) extracts of Arcopilus eremanthusum sp. nov. (CML3766) isolated from E. erythopappus. The fungi were identified using the sequences of the internal transcribed spacer (ITS), large subunit (LSU), and RNA polymerase II second largest subunit (RPB2). Antibacterial activity was determined using the minimum bactericidal concentration (MBC) method. Antioxidant activity was evaluated using free radical scavenging methods with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS
Publisher: Elsevier BV
Date: 31-05-2006
DOI: 10.1016/J.VETMIC.2005.12.010
Abstract: We developed an improved protocol for the electrotransformation of Corynebacterium pseudotuberculosis, testing variations of parameters in the procedures that are routinely used for the preparation of electrocompetent cells of this species, including (i) culture conditions, (ii) cell growth phase, (iii) electroporation solutions and (iv) quantity of plasmid DNA. We obtained the greatest efficiency of transformation when the cells were grown until the stationary phase and then washed with 10% glycerol electroporation solution. The transformation efficiency was inversely proportional to the quantity of plasmid DNA. The transformation efficiency reached 10(5) colony-forming units (cfu)/mug plasmid DNA. This protocol would be useful for genetic studies of C. pseudotuberculosis.
Publisher: Canadian Science Publishing
Date: 10-2010
DOI: 10.1139/W10-072
Abstract: Recombinant Penicillium griseoroseum strain 105 overproduces an extracellular pectin lyase (PL) under the transcriptional control of the strong gpdA promoter of Aspergillus nidulans . Our aim was to evaluate PL production by recombinant P. griseoroseum strain 105 in submerged fermentation system bioreactors BioFloIII and BioFloIV using 2 or 10 L working volumes under different growth conditions and to analyze the production of cellulase, polygalacturonase, pectin methylesterase, and protease. PL overproduction by recombinant P. griseoroseum strain 105 was 112 times higher than that of P. griseoroseum PG63 grown in sugarcane juice. Cellulases and proteases were not detected in the culture filtrate, and evaluation for extracellular proteins in the culture medium by SDS–PAGE showed the presence of a 36 kDa predominant band, similar to the molecular mass estimated from the nucleotide sequence of plg1 gene for PL of P. griseoroseum strain 105. This recombinant strain provides the advantage of PL production, which predominates over other extracellular proteins usually present in most commercial pectinase preparations, using sugarcane juice as a substrate of low cost.
Publisher: EDUFU - Editora da Universidade Federal de Uberlandia
Date: 2017
Publisher: FapUNIFESP (SciELO)
Date: 07-2019
Publisher: FapUNIFESP (SciELO)
Date: 03-2007
Publisher: Springer Science and Business Media LLC
Date: 11-2021
Publisher: Springer Science and Business Media LLC
Date: 18-12-2022
DOI: 10.1007/S00203-021-02682-Z
Abstract: Induratia spp. fungi have been poorly evaluated for their non-volatile secondary metabolites. In the present work, we evaluated the effects of non-volatile secondary metabolites released into the culture medium by Induratia spp. upon toxic alterations induced by Bothrops spp. venoms. B. atrox venom phospholipase was inhibited by Induratia spp. around 12 and 16%. The extracts of the two strains inhibited 12-25% of the hemolysis induced by B.moojeni venom. Thrombolysis was inhibited by 30-60% by the compounds present in both extracts. The coagulation induced by B. moojeni venom was prolonged by 26-41 s by the action of the extracts of I. coffeana. The fungal extracts did not exert any cytotoxic effect, nor did they induce any alteration in the other evaluated substrates show the potential use of non-volatile metabolites produced by the fungi evaluated as enzyme modulators, especially for proteases with a fundamental role in human hemostasis.
Publisher: Research Square Platform LLC
Date: 03-07-2023
DOI: 10.21203/RS.3.RS-3097277/V1
Abstract: Tomato is one of the most economically important crops in Brazil. However, several diseases limit tomato production, among which phytobacterial diseases are responsible for the greatest losses, such Ralstonia solanacearum , the pathogen most relevant to the crop. Endophytic fungi of the genus Muscodor are well known for the bioactivity of their volatile organic compounds (VOCs) against phytopathogens and also have the potential to promote plant growth, as in commum beans. Thus, in this study, we hypothesized that isolates of Muscodor spp. can be used for Ralstonia solanacearum control, and to increase tomato yield and total soluble solids content. To test our hypothesis, seven Muscodor coffeanum and two Muscodor sp. isolates associated with Coffea arabica were screened in vitro and in vivo. In vitro , using ided Petri dishes to evaluate the effect of VOCs, it was shown that the isolate of M. coffeanum CML 4009 promoted greater inhibitory effects on the growth of R. solanacearum race 3. In vivo , we inoculated seeds with Muscodor spp. isolates and later transplanted tomato seedlings into soil infested with R. solanacearum , and observed that the isolate CML 4009 reduced the severity of bacterial wilt. Muscodor isolates promoted an increase in fruit production and total soluble solids in tomatoes. Inoculation with Muscodor spp. is a plausible strategy for tomato seed treatment to control R. solanacearum diseases and increase tomato yield. The future of the M. coffeanum CML 4009 isolate as a biocontrol agent and plant growth promoter is promising.
Publisher: Oxford University Press (OUP)
Date: 21-11-2008
DOI: 10.1007/S10295-007-0277-6
Abstract: The pectin lyase (PL) is an industrially important enzyme since it is used for maceration and clarification in the process of fruit juice production in food industries. In order to increase the yields of pectin lyase we cloned the plg1 (pectin lyase 1) from Penicillium griseoroseum gene under the control of the strong constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) and the terminator region of the tryptophan synthetase (trpC) gene from Aspergillus nidulans (plasmid pAN52-Plg1) and transformed this construct into the P. griseoroseum strain PG63. One of the pAN52-Plg1 multi-copy transformants (strain 105) grown in culture medium containing glucose or sugar cane juice showed PL activities of 4,804 or 5,202 U ml(-1) respectively, which represented 57- and 132-fold increases. In addition, the apparent specific activity of PL produced by this strain was much higher than the one observed for a commercial pectinase preparation. Evaluation of the extracellular proteins in the culture supernatant of strain 105 by SDS-PAGE showed the presence of a clear and strong band of approximately 40 kDa that probably corresponds to PL. The enzyme yields reported here demonstrate that the system we developed is able to express pectin lyase at levels comparable to, or exceeding, previously reported data.
Publisher: FapUNIFESP (SciELO)
Date: 2022
DOI: 10.1590/1413-7054202246007322
Abstract: ABSTRACT Common bean (Phaseolus vulgaris L.) is a leguminous species of great economic and nutritional importance worldwide, and thus the development of management strategies to promote plant growth and increase grain yield of legumes is of great interest to farmers. The growth-promoting effects of the symbiosis of endophytic microorganisms in plants have been exploited in several crops. This study investigated the effects of Induratia sp. endophytic fungi on the growth and grain yield of seed-inoculated common bean plants. The experiments were conducted in a greenhouse during two growing seasons using a randomized complete block design with three replicates. Growth and yield-related parameters such as plant height, root length, leaf index, number of days for flowering, number of pods per plant, number of grains per pod, mass of 100 grains, and grain yield were evaluated. The grain yield of plants inoculated with Induratia coffeana strains CML 4018 and CML 4020 and Induratia sp. strain CML 4015 increased by 52.5%, 48.9%, and 56.4% compared to the non-inoculated control, respectively. Seed inoculation of common bean plants with endophytic fungi species of the genus Induratia showed a beneficial interaction as indicated by the recorded increase in grain yield. The present pioneering study could provide the means for developing crop management strategies for enhancing common bean crop productivity.
No related grants have been discovered for Patricia Cardoso.