ORCID Profile
0000-0002-3912-6095
Current Organisations
Ability Optics Pty Ltd
,
Australian National University
,
Garvan Institute of Medical Research
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Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Biomedical Instrumentation | Fluidisation and Fluid Mechanics | Interdisciplinary Engineering | Photonics and Electro-Optical Engineering (excl. Communications) | Biochemistry and cell biology | Biochemistry and Cell Biology | Biomechanical Engineering | Biomedical Engineering | Signal Transduction | Biological Physics | Protein Trafficking | Immunology | Engineering Instrumentation | Cellular Immunology | Protein trafficking | Cellular interactions (incl. adhesion matrix cell wall) | Mechanobiology | Cell Development, Proliferation and Death
ANZSRC Socio-Economic Objective (SEO)
Expanding Knowledge in the Biological Sciences | Expanding Knowledge in Engineering | Expanding Knowledge in Technology | Scientific Instruments | Immune System and Allergy |
Related Links
Publications
High Speed Temporal Modulation with Cascaded Fibre Delay Line and Micro-structured Mirrors
Publisher: OSA
Date: 2016
Dynamic axial control over optically levitating particles in air with an electrically-tunable variable-focus lens
Publisher: The Optical Society
Date: 30-06-2016
DOI: 10.1364/BOE.7.002902
Resolving Stable Axial Trapping Points of Nanowires in an Optical Tweezers Using Photoluminescence Mapping
Publisher: American Chemical Society (ACS)
Date: 14-02-2013
DOI: 10.1021/NL304607V
Abstract: Axially resolved microphotoluminescence mapping of semiconductor nanowires held in an optical tweezers reveals important new experimental information regarding equilibrium trapping points and trapping stability of high aspect ratio nanostructures. In this study, holographic optical tweezers are used to scan trapped InP nanowires along the beam direction with respect to a fixed excitation source and the luminescent properties are recorded. It is observed that nanowires with lengths on the range of 3-15 μm are stably trapped near the tip of the wire with the long segment positioned below the focus in an inverted trapping configuration. Through the use of trap multiplexing we investigate the possibility of improving the axial stability of the trapped nanowires. Our results have important implication for applications of optically assisted nanowire assembly and optical tweezers based scanning probes microscopy.
Probing the Nonlinear Optical Response of Nanosuspensions
Publisher: OSA
Date: 2009
Structured back focal plane interferometry (S-BFPI)
Publisher: SPIE
Date: 30-12-2019
DOI: 10.1117/12.2539556
Controlled three-dimensional manipulation of vanadium oxide nanotubes with optical tweezers
Publisher: AIP Publishing
Date: 15-12-2008
DOI: 10.1063/1.3039407
Abstract: We present a direct nanotube-microsphere tagging technique for the controlled three-dimensional (3D) manipulation and transportation of vanadium oxide nanotubes (VOx-NTs) with optical tweezers. The high scattering and absorptive nature of the VOx-NTs preclude the 3D optical trapping of such nanostructures. VOx-NTs are adhered to 3-aminopropyl-triethoxysilane functionalized silica microspheres, which act as handles for indirectly manipulating and transporting the nanotubes in three dimensions with optical tweezers. The optical tweezers can also operate as optical scissors that can remove the dielectric handles and trim these nanotubes. This technique may be extended to the optical manipulation of nanotubes of any material.
DHMI: dynamic holographic microscopy interface
Publisher: SPIE
Date: 09-12-2016
DOI: 10.1117/12.2242948
Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 2010
Integrated elastic microscope device
Publisher: SPIE
Date: 09-03-2015
DOI: 10.1117/12.2076100
Label-free dynamic volumetric imaging of deforming giant unilamellar vesicles under micro-flows
Publisher: OSA
Date: 2017
Mapping optical process in semiconductor nanowires using dynamic optical tweezers
Publisher: SPIE
Date: 09-10-2012
DOI: 10.1117/12.928558
Collinear non-diffracting beams: classification and properties
Publisher: SPIE
Date: 11-02-2010
DOI: 10.1117/12.840390
Application of computational optics in moldless lenses
Publisher: OSA
Date: 2017
Novel stenotic microchannels to study thrombus formation in shear gradients: Influence of shear forces and human platelet-related factors
Publisher: MDPI AG
Date: 18-06-2019
DOI: 10.3390/IJMS20122967
Abstract: Thrombus formation in hemostasis or thrombotic disease is initiated by the rapid adhesion, activation, and aggregation of circulating platelets in flowing blood. At arterial or pathological shear rates, for ex le due to vascular stenosis or circulatory support devices, platelets may be exposed to highly pulsatile blood flow, while even under constant flow platelets are exposed to pulsation due to thrombus growth or changes in vessel geometry. The aim of this study is to investigate platelet thrombus formation dynamics within flow conditions consisting of either constant or variable shear. Human platelets in anticoagulated whole blood were exposed ex vivo to collagen type I-coated microchannels subjected to constant shear in straight channels or variable shear gradients using different stenosis geometries (50%, 70%, and 90% by area). Base wall shears between 1800 and 6600 s−1, and peak wall shears of 3700 to 29,000 s−1 within stenoses were investigated, representing arterial-pathological shear conditions. Computational flow-field simulations and stenosis platelet thrombi total volume, average volume, and surface coverage were analysed. Interestingly, shear gradients dramatically changed platelet thrombi formation compared to constant base shear alone. Such shear gradients extended the range of shear at which thrombi were formed, that is, platelets became hyperthrombotic within shear gradients. Furthermore, in idual healthy donors displayed quantifiable differences in extent/formation of thrombi within shear gradients, with implications for future development and testing of antiplatelet agents. In conclusion, here, we demonstrate a specific contribution of blood flow shear gradients to thrombus formation, and provide a novel platform for platelet functional testing under shear conditions.
High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope.
Publisher: Wiley
Date: 10-04-2018
Abstract: In this study, we introduce two key improvements that overcome limitations of existing polygon scanning microscopes while maintaining high spatial and temporal imaging resolution over large field of view (FOV). First, we proposed a simple and straightforward means to control the scanning angle of the polygon mirror to carry out photomanipulation without resorting to high speed optical modulators. Second, we devised a flexible data s ling method directly leading to higher image contrast by over 2-fold and digital images with 100 megapixels (10 240 × 10 240) per frame at 0.25 Hz. This generates sub-diffraction limited pixels (60 nm per pixels over the FOV of 512 μm) which increases the degrees of freedom to extract signals computationally. The unique combined optical and digital control recorded fine fluorescence recovery after localized photobleaching (r ~10 μm) within fluorescent giant unilamellar vesicles and micro-vascular dynamics after laser-induced injury during thrombus formation in vivo. These new improvements expand the quantitative biological-imaging capacity of any polygon scanning microscope system.
Holo-UNet: hologram-to-hologram neural network restoration for high fidelity low light quantitative phase imaging of live cells
Publisher: Optica Publishing Group
Date: 09-09-2020
DOI: 10.1364/BOE.395302
Abstract: Intensity shot noise in digital holograms distorts the quality of the phase images after phase retrieval, limiting the usefulness of quantitative phase microscopy (QPM) systems in long term live cell imaging. In this paper, we devise a hologram-to-hologram neural network, Holo-UNet, that restores high quality digital holograms under high shot noise conditions (sub-mW/cm 2 intensities) at high acquisition rates (sub-milliseconds). In comparison to current phase recovery methods, Holo-UNet denoises the recorded hologram, and so prevents shot noise from propagating through the phase retrieval step that in turn adversely affects phase and intensity images. Holo-UNet was tested on 2 independent QPM systems without any adjustment to the hardware setting. In both cases, Holo-UNet outperformed existing phase recovery and block-matching techniques by ∼ 1.8 folds in phase fidelity as measured by SSIM. Holo-UNet is immediately applicable to a wide range of other high-speed interferometric phase imaging techniques. The network paves the way towards the expansion of high-speed low light QPM biological imaging with minimal dependence on hardware constraints.
Nonlinear optical response of colloidal suspensions
Publisher: Optica Publishing Group
Date: 04-06-2009
DOI: 10.1364/OE.17.010277
Abstract: We experimentally probed the nonlinear optical response of aqueous nano-colloidal suspensions to provide a test of the theoretical approaches that have been proposed for the nonlinearity, namely an exponential model, an artificial Kerr medium, and a non-ideal gas model. The best agreement with experiment is found using the non-ideal gas model for the colloidal suspension which in turn can be used to infer values for the second virial coefficient of the medium and the nonlinear coefficients.
Automated fourier space region-recognition filtering for off-axis digital holographic microscopy
Publisher: The Optical Society
Date: 22-07-2016
DOI: 10.1364/BOE.7.003111
Design and fabrication of a passive droplet dispenser for portable high resolution imaging system
Publisher: Springer Science and Business Media LLC
Date: 27-01-2017
DOI: 10.1038/SREP41482
Abstract: Moldless lens manufacturing techniques using standard droplet dispensing technology often require precise control over pressure to initiate fluid flow and control droplet formation. We have determined a series of interfacial fluid parameters optimised using standard 3D printed tools to extract, dispense and capture a single silicone droplet that is then cured to obtain high quality lenses. The dispensing process relies on the recapitulation of liquid dripping action (Rayleigh-Plateau instability) and the capturing method uses the interplay of gravitational force, capillary forces and liquid pinning to control the droplet shape. The key advantage of the passive lens fabrication approach is rapid scale-up using 3D printing by avoiding complex dispensing tools. We characterise the quality of the lenses fabricated using the passive approach by measuring wavefront aberration and high resolution imaging. The fabricated lenses are then integrated into a portable imaging system a wearable thimble imaging device with a detachable camera housing, that is constructed for field imaging. This paper provides the full exposition of steps, from lens fabrication to imaging platform, necessary to construct a standalone high resolution imaging system. The simplicity of our methodology can be implemented using a regular desktop 3D printer and commercially available digital imaging systems.
Imaging Platelet Processes and Function—Current and Emerging Approaches for Imaging in vitro and in vivo
Publisher: Frontiers Media SA
Date: 31-01-2020
Design and fabrication of micro-optical elements for the generation of various novel beams and its applications in optical tweezers
Publisher: SPIE
Date: 26-01-2006
DOI: 10.1117/12.667706
Soluble GPVI is elevated in injured patients: Shedding is mediated by fibrin activation of GPVI
Publisher: American Society of Hematology
Date: 02-2018
DOI: 10.1182/BLOODADVANCES.2017011171
Abstract: Soluble GPVI is elevated in patients with thermal injury with sepsis, and sGPVI levels augment severity score prediction of mortality. The GPVI ligand, fibrin, induces GPVI shedding without requirement for platelet activation or signaling
Quasi-Bessel hollow beam as optical guide for micro-particles
Publisher: SPIE
Date: 12-09-2013
DOI: 10.1117/12.2026654
PL mapping and optimized optical trapping of nanowires SLM beam shaping
Publisher: IEEE
Date: 12-2012
Far field interference measurements of vortex light fields in optical trapping
Publisher: SPIE
Date: 08-02-2007
DOI: 10.1117/12.702215
Observation of three-dimensional optical stacking of microparticles using a single Laguerre–Gaussian beam
Publisher: AIP Publishing
Date: 17-12-2003
DOI: 10.1063/1.1635079
Abstract: Optical stacking of microparticles has been demonstrated earlier using a single focused Gaussian beam, multiple beams from Laguerre–Gaussian (LG) interference patterns, and Bessel beams. In this letter, we demonstrate that a single focused LG beam has the ability to optically stack multiple high-index microparticles around the intensity annular rings of the LG beam, and thus form a three-dimensional structure. Due to the symmetrically circular shape of the LG, we have been able to stack particles in a circular manner. Hence we propose that this technique of stacking can be extended to optical fields of designed shaped such that the stacking microparticles will be organized according to the shaped of the beam intensity. This is an alternative method to obtain a desired three-dimensional crystalline structure, where shaping the optical vortices beam is used instead of using multiple beams.
Dynamic control over field of view in polygon mirror-based laser scanning multiphoton microscope
Publisher: OSA
Date: 2017
Adaptive spatial filtering for off-axis digital holographic microscopy based on region recognition approach with iterative thresholding
Publisher: SPIE
Date: 09-12-2016
DOI: 10.1117/12.2242876
PScan 1.0: flexible software framework for polygon based multiphoton microscopy
Publisher: SPIE
Date: 09-12-2016
DOI: 10.1117/12.2242954
Fabrication and operation of GRIN probes for in vivo fluorescence cellular imaging of internal organs in small animals
Publisher: Springer Science and Business Media LLC
Date: 05-07-2012
Optical coherence measurements of vortex light fields using optically manipulated micro-apertures
Publisher: SPIE
Date: 13-09-2007
DOI: 10.1117/12.734131
A compact multi-trap optical tweezer system based on CD-ROM technologies
Publisher: SPIE
Date: 25-08-2017
DOI: 10.1117/12.2273674
Hollow Bessel-like beam as an optical guide for a stream of microscopic particles
Publisher: The Optical Society
Date: 04-12-2013
DOI: 10.1364/OE.21.030492
Raster adaptive optics for video rate laser scanning microscopy with large field of view correction
Publisher: Optica Publishing Group
Date: 2020
DOI: 10.1364/CLEO_AT.2020.AM2I.1
Abstract: We propose a raster scanning adaptive optics method that uses digital image segmentation and a low-resolution deformable mirror with a maximum of 50 wavefront masks, which removes spatially varying aberrations (both s le and lens) across a field of view of 0.8 mm at 500 ms.
Resolving interparticle position and optical forces along the axial direction using optical coherence gating
Publisher: AIP Publishing
Date: 06-12-2010
DOI: 10.1063/1.3519976
Abstract: In this paper, we demonstrate the use of coherence gating to resolve particle positions and forces in the axial direction. Through coherence gating, particle displacements and interparticle separations can be resolved with a high signal-to-noise ratio. We achieved both high depth resolvability (10−6m) and weak optical force (10−15 N) measurements in an optical trapping system using a low coherence interferometry system. Trap stiffness as low as 1.46 fN μm−1 was measured. This technique is well-suited for the direct visualization of interparticle optical-mechanical interactions.
Multiple optical line traps using a single phase-only rectangular ridge
Publisher: Springer Science and Business Media LLC
Date: 26-05-2005
Coherent optical scattering and interferometry microscopy for functional imaging of thrombus
Publisher: Optica Publishing Group
Date: 2020
DOI: 10.1364/MICROSCOPY.2020.MTH3A.3
Abstract: In this work, we propose a label-free COSI system to investigating morphological changes and platelet-platelet interactions within a thrombus during embolism events to interrogate prothrombotic events within a microfluidics channel under flow.
Optical trapping using ultrashort 12.9fs pulses
Publisher: SPIE
Date: 28-08-2008
DOI: 10.1117/12.795085
Trust and consumer risk perceptions regarding BSE and chronic wasting disease
Publisher: Wiley
Date: 08-11-2017
DOI: 10.1002/AGR.21524
Optically trapped and controlled microapertures for studies of spatial coherence in an arbitrary light field
Publisher: AIP Publishing
Date: 25-06-2007
DOI: 10.1063/1.2751590
Abstract: By controlling the rotation rate of a trapped birefringent particle with an optically applied torque, the authors introduce a miniscule wave front deformation at a specific location within an arbitrary light field, with the particle acting as an optical microdiffuser. A trapped birefringent particle and a trapped silica microsphere are positioned to form Young’s double slit experiment within a probe light field. The far-field interference from the diffracted optical fields from these particles enable the authors to infer the relative spatial coherence between these local s ling points. With multiple trapped particles, the authors may perform multipoint coherence analysis of a light field.
In situ retrieval and correction of aberrations in moldless lenses using Fourier ptychography
Publisher: The Optical Society
Date: 25-01-2018
DOI: 10.1364/OE.26.002708
Optical vortex beam shaping by use of highly efficient irregular spiral phase plates for optical micromanipulation
Publisher: Optica Publishing Group
Date: 08-2004
DOI: 10.1364/OL.29.001796
Abstract: Optical dark traps such as Laguerre-Gaussian beams, modulated optical vortices, and high-order Bessel beams have been used in the micromanipulation of microparticles. Such optical traps are highly versatile, as they are able to trap both high- and low-index microparticles as well as to set them into rotation by use of the orbital angular momentum of light. Holography has been widely used to modulate the shape of an optical vortex for new optical traps. We show that, by designing the shape of a spiral phase plate and using electron-beam lithography for fabrication, one can modulate the litude and the phase of an optical vortex with respect to the specific shape of the spiral phase plate as required. Furthermore, to the best of our knowledge this is the first report of transferring orbital angular momentum from a spiral phase plate to an absorptive microparticle in an experiment. Hence, with this technique, optical dark traps can easily be designed and fabricated.
Modified inverted selective plane illumination microscopy for sub-micrometer imaging resolution in polydimethylsiloxane soft lithography devices
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0LC00598C
Abstract: Sub-micrometer lightsheet imaging of live fibroblast cell in PDMS microdevices by m-iSPIM.
Construction of three-dimensional microstructure using optical beam of designed vortex-shape
Publisher: SPIE
Date: 18-10-2004
DOI: 10.1117/12.551005
Dielectric enhanced nanoparticles for three-dimensional optical manipulation
Publisher: SPIE
Date: 20-08-2009
DOI: 10.1117/12.825456
Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
Publisher: AIP Publishing
Date: 28-02-2011
DOI: 10.1063/1.3554415
Abstract: We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope s le was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
Mapping the progression of malaria infected erythrocytes with holographic microscopy
Publisher: IEEE
Date: 08-2016
Optical Separation of Cells on Potential Energy Landscapes: Enhancement With Dielectric Tagging
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 2007
Resolving inter-particle position and optical forces along the axial direction using optical coherence gating
Publisher: OSA
Date: 2011
Direct fabrication of silicone lenses with 3D printed parts
Publisher: SPIE
Date: 24-11-2016
DOI: 10.1117/12.2242967
Optical Trapping Takes Shape: The Use of Structured Light Fields
Publisher: Elsevier
Date: 2008
Computational single-objective scanning light sheet (cSOLS)
Publisher: AIP Publishing
Date: 08-2022
DOI: 10.1063/5.0091615
Abstract: Single-objective scanning light sheet (SOLS) imaging has fueled major advances in volumetric bioimaging because it supports low phototoxic, high-resolution imaging over an extended period. The remote imaging unit in the SOLS does not use a conventional epifluorescence image detection scheme (a single tube lens). In this paper, we propose a technique called the computational SOLS (cSOLS) that achieves light sheet imaging without the remote imaging unit. Using a single microlens array after the tube lens (lightfield imaging), the cSOLS is immediately compatible with conventional epifluorescence detection. The core of cSOLS is a Fast Optical Ray (FOR) model. FOR generates 3D imaging volume (40 × 40 × 14 µm3) using 2D lightfield images taken under SOLS illumination within 0.5 s on a standard central processing unit (CPU) without multicore parallel processing. In comparison with traditional lightfield retrieval approaches, FOR reassigns fluorescence photons and removes out-of-focus light to improve optical sectioning by a factor of 2, thereby achieving a spatial resolution of 1.59 × 1.92 × 1.39 µm3. cSOLS with FOR can be tuned over a range of oblique illumination angles and directions and, therefore, paves the way for next-generation SOLS imaging. cSOLS marks an important and exciting development of SOLS imaging with computational imaging capabilities.
Label-free optical scattering and interferometry microscopy for functional imaging of thrombus
Publisher: SPIE
Date: 30-12-2019
DOI: 10.1117/12.2539533
Upright aNd inverted polygon microscope (UNI-SCOPE)
Publisher: SPIE
Date: 30-12-2019
DOI: 10.1117/12.2539970
Fibrin exposure triggers αIIbβ3‐independent platelet aggregate formation, ADAM10 activity and glycoprotein VI shedding in a charge‐dependent manner
Publisher: Elsevier BV
Date: 06-2020
DOI: 10.1111/JTH.14797
Structured Back Focal Plane Interferometry (SBFPI)
Publisher: Springer Science and Business Media LLC
Date: 30-12-2019
DOI: 10.1038/S41598-019-56199-Z
Abstract: Back focal plane interferometry (BFPI) is one of the most straightforward and powerful methods for achieving sub-nanometer particle tracking precision at high speed (MHz). BFPI faces technical challenges that prohibit tunable expansion of linear detection range with minimal loss to sensitivity, while maintaining robustness against optical aberrations. In this paper, we devise a tunable BFPI combining a structured beam (conical wavefront) and structured detection (annular quadrant photodiode). This technique, which we termed Structured Back Focal Plane Interferometry (SBFPI), possesses three key novelties namely: extended tracking range, low loss in sensitivity, and resilience to spatial aberrations. Most importantly, the conical wavefront beam preserves the axial Gouy phase shift and lateral beam waist that can then be harnessed in a conventional BFPI system. Through a series of experimental results, we were able to tune detection sensitivity and detection range over the SBFPI parameter space. We also identified a figure of merit based on the experimental optimum that allows us to identify optimal SBPFI configurations that balance both range and sensitivity. In addition, we also studied the resilience of SBFPI against asymmetric spatial aberrations (astigmatism of up to 0.8 λ) along the lateral directions. The simplicity and elegance of SBFPI will accelerate its dissemination to many associated fields in optical detection, interferometry and force spectroscopy.
Long distance beam propagation in colloidal suspensions: comparison between theory and experiment
Publisher: SPIE
Date: 28-08-2008
DOI: 10.1117/12.796243
Interference from multiple trapped colloids in an optical vortex beam
Publisher: Optica Publishing Group
Date: 2006
DOI: 10.1364/OE.14.007436
Abstract: Laguerre-Gaussian (LG) beams are important in optical micromanipulation. We show that optically trapped microparticles within a monochromatic LG beam may lead to the formation of unique intensity patterns in the far field due to multiple interference of the forward scattered light from each particle. Trapped colloids create far field interference that exhibits distinct spiral wave patterns that are directly correlated to the helicity of the LG beam. Using two trapped particles, we demonstrate the first microscopic version of a Young's slits type experiment and detect the azimuthal phase variation around the LG beam circumference. This novel technique may be implemented to study the relative phase and spatial coherence of two points in trapping light fields with arbitrary wavefronts.
Optical steering of high and low index microparticles by manipulating an off-axis optical vortex
Publisher: IOP Publishing
Date: 20-11-2004
Optical trapping and micromanipulation using microbeam shaping created by micro-optical elements
Publisher: SPIE
Date: 07-02-2005
DOI: 10.1117/12.571969
Shaping self-imaging bottle beams with modified quasi-Bessel beams
Publisher: The Optical Society
Date: 08-04-2014
DOI: 10.1364/OL.39.002278
Intravital Microscopic Interrogation of Peripheral Taste Sensation
Publisher: Springer Science and Business Media LLC
Date: 02-03-2015
DOI: 10.1038/SREP08661
Stick-on microscope for smartphones
Publisher: SPIE
Date: 28-02-2014
DOI: 10.1117/12.2032620
Coherent Optical Scattering and Interferometry (COSI) Microscopy for Morphological Imaging of Thrombus
Publisher: Optica Publishing Group
Date: 2020
DOI: 10.1364/CLEO_AT.2020.AM1I.4
Abstract: In this work, we propose a label-free COSI system to quantify morphological changes and platelet activity along non-patterned collagen fibers within millisecond in microfluidics channels under flow at sub-platelet imaging resolution.
Label-free multimodal quantitative imaging flow assay for intrathrombus formation in vitro
Publisher: Elsevier BV
Date: 03-2021
Optical Nonlinearity of a Colloidal “Non-Ideal Gas” of Nano-Suspensions
Publisher: OSA
Date: 2009
High efficient micro-optical elements for optical vortices in optical manipulation
Publisher: OSA
Date: 2005
Optical toolkits forin vivodeep tissue laser scanning microscopy: a primer
Publisher: IOP Publishing
Date: 29-05-2018
Selective optical trapping using optical beams with fractional helical phase
Publisher: SPIE
Date: 18-10-2004
DOI: 10.1117/12.551016
Experimental observation of pure helical phase interference using moiré fringes generated from holograms with dislocations
Publisher: IOP Publishing
Date: 15-04-2004
Combined Scattering, Interferometric and Fluorescence Oblique Illumination for Live Cell Nanoscale Imaging
Publisher: Cold Spring Harbor Laboratory
Date: 22-07-2022
DOI: 10.1101/2022.07.22.501104
Abstract: To determine the molecular and/or mechanical basis of cell migration using live cell imaging tools, it is necessary to correlate multiple 3D spatiotemporal events simultaneously. Fluorescence nanoscopy and label free nanoscale imaging can complement each other by providing both molecular specificity and structural dynamics of sub-cellular structure. In doing so, a combined imaging system would permit quantitative 3D spatial temporal detail of in idual cellular components. In this paper, we empirically determined a series of optimal azimuthal scanning angles and rotating beam to achieve simultaneous and label-free nanoscale and fluorescence imaging. Label-free nanoscale imaging here refers to interferometric, brightfield (BF) and darkfield (DF) rotating coherence scattering (ROCS) microscopy, while fluorescence refers to high inclined Laminated Oblique (HiLO) and total internal reflection fluorescence (TIRF) imaging. The combined capabilities of interferometric, scattering and fluorescence imaging enables (1) the identification of molecular targets (substrate or organelle), (2) quantification of 3D cell morphodynamics, and (3) tracking of intracellular organelles in 3D. This combined imaging tool was then used to characterize migrating platelets and adherent endothelial cells, both critical to the process of infection and wound healing. The combined imaging results of over ∼1000 platelets, suggested that serum albumin (bovine) was necessary for platelets to migrate and scavenge fibrin/fibrinogen. Furthermore, we determine new asynchronous membrane fluctuations between the leading and rear edge of a migrating platelet. We further demonstrated that interferometric imaging permitted the quantification of mitochondria dynamics on lung microvascular cells (HMVEC). Our data suggests that axial displacement of mitochondria is minimized when it is closer to the nucleus or the leading edge of a cell membrane that exhibits retrograde motion. Taken together, this combined imaging platform has proven to quantify multiple spatial temporal events of a migrating cell, that will undoubtedly open ways to new quantitative correlative nanoscale live cell imaging.
Noise reduction in ultra-low light digital holographic microscopy using neural networks
Publisher: SPIE
Date: 30-12-2019
DOI: 10.1117/12.2539548
Optical nonlinearity of liquid nanosuspensions: Kerr versus exponential model
Publisher: SPIE
Date: 20-08-2009
DOI: 10.1117/12.825865
Coherent Optical Scattering and Interferometry (COSI) for label-free multiparameter quantitative imaging of intra-thrombus stability in vitro
Publisher: Cold Spring Harbor Laboratory
Date: 20-08-2020
DOI: 10.1101/2020.08.17.254292
Abstract: Although existing microfluidics in vitro assays recapitulate blood vessel microenvironment using surface-immobilized agonists under biofluidic flows, these assays do not quantify intra-thrombus mass and activities of adhesive platelets at agonist margin and uses fluorescence labeling, therefore limiting clinical translation potential. Here, we describe a real time label-free in vitro quantitative imaging flow assay called C oherent O ptical S cattering and phase I nterferometry (COSI) that evaluates both intra-thrombus and adhesive-only platelet dynamics using only changes in refractive index. By combining coherent optical scattering and optical interferometry, we evaluated and quantified both intra-thrombus mass with picogram accuracy and adhesive platelet-only events/dynamics with high spatial-temporal resolution (400 nm/s) under fluid shear stress using only changes in refractive index. Using oblique illumination, COSI provide a ∼ 4 µm thin axial slice that quantifies the magnitude of physical of surface adhesive platelets (spreading, adhesion and consolidation) in a developing thrombus without labelling under fluid shear stress. We achieve real time visualization of recruitment of single platelet into thrombus and further correlate it to the developing mass of a thrombus. The adhesive platelet activity exhibit stabilized surface activity of around 2 µm/s and intra-thrombus mass exchange were balanced at around 1 picogram after treatment of a broad range metalloproteinase inhibitor (250 µM GM6001). The combination of phase imaging with transmitted light and backscattering imaging via oblique illumination in COSI unpicked intra-thrombus mass and adhesive platelet-only activity events at picogram and sub-micrometer precision with millisecond time resolution under fluid shear stress. COSI maps the longitudinal time dynamics of adhesive platelets along changing thrombus mass under metalloproteinase inhibition, and demonstrates potential for real-time correlative microfluidic label-free imaging for flow-dependent biological adhesive events.
Construction and calibration of an optical trap on a fluorescence optical microscope
Publisher: Springer Science and Business Media LLC
Date: 12-2007
Abstract: The application of optical traps has come to the fore in the last three decades. They provide a powerful, sterile and noninvasive tool for the manipulation of cells, single biological macromolecules, colloidal microparticles and nanoparticles. An optically trapped microsphere may act as a force transducer that is used to measure forces in the piconewton regime. By setting up a well-calibrated single-beam optical trap within a fluorescence microscope system, one can measure forces and collect fluorescence signals upon biological systems simultaneously. In this protocol, we aim to provide a clear exposition of the methodology of assembling and operating a single-beam gradient force trap (optical tweezers) on an inverted fluorescence microscope. A step-by-step guide is given for alignment and operation, with discussion of common pitfalls.
Fabricating low cost and high performance elastomer lenses using hanging droplets
Publisher: The Optical Society
Date: 24-04-2014
DOI: 10.1364/BOE.5.001626
Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption
Publisher: Elsevier BV
Date: 03-2021
Experimental study of holographic generation of fractional Bessel beams
Publisher: Optica Publishing Group
Date: 2004
DOI: 10.1364/AO.43.000122
Abstract: We demonstrate the experimental generation of a fractional Bessel beam by holographic means. Such fractional modes of Bessel beams possess an intrinsic opening gap across concentric intensity rings on propagation. We also show that the opening gaps within the fractional modes are diffraction free for a working distance while a fractional helical wave front is maintained.
Compact flexible multi-pass rotary delay line using spinning micro-machined mirrors
Publisher: Springer Science and Business Media LLC
Date: 24-08-2017
DOI: 10.1038/S41598-017-09576-5
Abstract: We propose a new method to extend the path length tunability of rotary delay-lines. This method was shown to achieve a duty cycle of % and repetition rates of over 40 kHz. The new method relies on a new multi-segmented micro-machined mirror and serial injection of a single reflection onto separate segments of this mirror. The tunability is provided by the relative positioning of each reflective point on the mirror segments. There are two distinct modes of operation: synchronous and asynchronous. By simply manipulating the spatial position of the returning paths over the respective mirror segments, we can switch between increasing the repetition rate (asynchronous mode) or the total delay path (synchronous mode). We experimentally demonstrated up to 8 m/s scans with repetition rates of up to 42.7 kHz. Furthermore, we present numerical simulations of 18 reflection points to illustrate possibility of achieving a scan speed of up to 80 m/s. Through intermediate combinations of synchronous and asynchronous operation modes with 4 or more passes, we also show that the system can simultaneously increase both repetition rate and scan depth.
Quantifying Embolism: Label-Free Volumetric Mapping of Thrombus Structure and Kinesis in a Microfluidic System with Optical Holography
Publisher: Wiley
Date: 31-07-2018
In-fiber common-path optical coherence tomography using a conical-tip fiber
Publisher: Optica Publishing Group
Date: 05-02-2009
DOI: 10.1364/OE.17.002375
Abstract: Common-path optical coherence tomography (CPOCT) is known to reduce group velocity dispersion and polarization mismatch between the reference and the s le arm as both arms share the same physical path. Existing implementations of CPOCT typically require one to incorporate an additional cover glass within the beam path of the s le arm to provide a reference signal. In this paper, we aim to further reduce this step by directly making use of the back-reflected signal, arising from a conical lens-tip fiber, as a reference signal. The conical lens, which is directly manufactured onto the optical fiber tip via a simple selective-chemical etching process, fulfils two functions acting as both the imaging lens and the self-aligning reference plane. We use a Fourier-domain OCT system to demonstrate the feasibility of this technique upon biological tissue. An in-fiber CPOCT technique may prove potentially useful in endoscopic OCT imaging.
Optical Nanomanipulation and Structured-Beam Optical Traps
Publisher: Elsevier
Date: 2019
Nonlinear optical dynamics in nonideal gases of interacting colloidal nanoparticles
Publisher: American Physical Society (APS)
Date: 03-11-2009
Flexible polygon‐mirror based laser scanning microscope platform for multiphoton in‐vivo imaging
Publisher: Wiley
Date: 06-02-2017
Abstract: Commercial microscopy systems make use of tandem scanning i.e. either slow or fast scanning. We constructed, for the first time, an advanced control system capable of delivering a dynamic line scanning speed ranging from 2.7 kHz to 27 kHz and achieve variable frame rates from 5 Hz to 50 Hz (512 × 512). The dynamic scanning ability is digitally controlled by a new customized open-source software named PScan1.0. This permits manipulation of scanning rates either to gain higher fluorescence signal at slow frame rate without increasing laser power or increase frame rates to capture high speed events. By adjusting imaging speed from 40 Hz to 160 Hz, we capture a range of calcium waves and transient peaks from soma and dendrite of single fluorescence neuron (CAL-520AM). Motion artifacts arising from respiratory and cardiac motion in small animal imaging reduce quality of real-time images of single cells in-vivo. An image registration algorithm, integrated with PScan1.0, was shown to perform both real time and post-processed motion correction. The improvement is verified by quantification of blood flow rates. This work describes all the steps necessary to develop a high performance and flexible polygon-mirror based multiphoton microscope system for in-vivo biological imaging.
Raster adaptive optics for video rate aberration correction and large FOV multiphoton imaging
Publisher: Optica Publishing Group
Date: 23-01-2020
DOI: 10.1364/BOE.377044
Abstract: Removal of complex aberrations at millisecond time scales over millimeters in distance in multiphoton laser scanning microscopy limits the total spatiotemporal imaging throughput for deep tissue imaging. Using a single low resolution deformable mirror and time multiplexing (TM) adaptive optics, we demonstrate video rate aberration correction (5 ms update rate for a single wavefront mask) for a complex heterogeneous distribution of refractive index differences through a depth of up to 1.1 mm and an extended imaging FOV of up to 0.8 mm, with up to 167% recovery of fluorescence intensity 335 µm from the center of the FOV. The proposed approach, termed raster adaptive optics (RAO), integrates image-based aberration retrieval and video rate removal of arbitrarily defined regions of dominant, spatially varied wavefronts. The extended FOV was achieved by demonstrating rapid recovery of up to 50 distinct wavefront masks at 500 ms update rates that increased imaging throughput by 2.3-fold. Because RAO only requires a single deformable mirror with image-based aberration retrieval, it can be directly implemented on a standard laser scanning multiphoton microscope.
Combined Scattering, Interferometric, and Fluorescence Oblique Illumination for Live Cell Nanoscale Imaging
Publisher: American Chemical Society (ACS)
Date: 14-11-2022
Experimental observation of optical vortex evolution in a Gaussian beam with an embedded fractional phase step
Publisher: Elsevier BV
Date: 09-2004
Targeted broadband ultraviolet B phototherapy improves disorders characterized by increased dermal matrix
Publisher: Oxford University Press (OUP)
Date: 12-2009
Direct electron-beam writing of continuous spiral phase plates in negative resist with high power efficiency for optical manipulation
Publisher: AIP Publishing
Date: 06-12-2004
DOI: 10.1063/1.1830678
Abstract: Laser beams propagating in Laguerre-Gaussian (LG) modes are of considerable interest due to their widespread applications in the areas of optical manipulation of microparticles, quantum entanglement of photons, nonlinear optics, optical vortex interactions, and atomic studies. However, the proliferation of LG beams has been h ered due to the absence of reliable and reproducible fabrication technologies in producing the required optical elements for their generation. In this letter, we describe a simple, reliable, and reproducible fabrication technique for a micron-sized spiral phase plate with high power efficiency (80%–90%) and good beam uniformity. This facilitates the widespread use of LG beams in various applications: as an ex le the fabricated elements can easily and readily be incorporated into an existing optical trapping system with minimum modification.
Improving focal photostimulation of cortical neurons with pre-derived wavefront correction
Publisher: Frontiers Media SA
Date: 05-2017
Peptide Programmed Hydrogels as Safe Sanctuary Microenvironments for Cell Transplantation
Publisher: Wiley
Date: 19-12-2019
Ultrahigh-resolution optical coherence elastography through a micro-endoscope: towards in vivo imaging of cellular-scale mechanics
Publisher: The Optical Society
Date: 20-10-2017
DOI: 10.1364/BOE.8.005127
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