ORCID Profile
0000-0002-7552-6184
Current Organisation
Universidad de Sevilla
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Publisher: Springer Science and Business Media LLC
Date: 02-10-2018
DOI: 10.1038/S41467-018-06156-7
Abstract: The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
Publisher: Springer Science and Business Media LLC
Date: 04-2016
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C5LC01012H
Abstract: This review presents the state of the art of active microfluidic droplet generation concepts.
Publisher: Springer Science and Business Media LLC
Date: 11-08-2022
DOI: 10.1038/S41467-022-32434-6
Abstract: The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from in idual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in in idual single crystals, a technique we refer to as multi-hit SFX.
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C6LC01435F
Abstract: This review presents the fundamentals of different active methods for sorting droplets in microfluidics.
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C4LC01139B
Abstract: We report a new technology using acoustofluidic to achieve controllable manipulation of the size of the bubbles formed.
No related grants have been discovered for Alfonso Ganan-Calvo.