ORCID Profile
0000-0003-4736-5106
Current Organisations
RMIT University Bundoora Campus
,
RMIT University
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Publisher: Hindawi Limited
Date: 03-04-2019
DOI: 10.1111/JFPP.13939
Publisher: Elsevier BV
Date: 2019
Publisher: Codon Publications
Date: 30-09-2018
DOI: 10.3920/QAS2017.1216
Publisher: Hindawi Limited
Date: 13-02-2018
DOI: 10.1111/JFPP.13606
Publisher: Springer Science and Business Media LLC
Date: 26-04-2023
Publisher: MDPI AG
Date: 26-10-2022
Abstract: The use of natural ingredients for managing diabetes is becoming more popular in recent times due to the several adverse effects associated with synthetic antidiabetic medications. In this study, we investigated the in vitro antidiabetic potential (through inhibition of α-glucosidase (AG) and α-amylase (AA)) of hydrolysates from lupin proteins pretreated with ultrasound and hydrolyzed using alcalase (ACT) and flavourzyme (FCT). We further fractionated ACT and FCT into three molecular weight fractions. Unfractionated ACT and FCT showed significantly (p 0.05) higher AG (IC50 value = 1.65 mg/mL and 1.91 mg/mL) and AA (IC50 value = 1.66 mg/mL and 1.98 mg/mL) inhibitory activities than their ultrafiltrated fractions, where lower IC50 values indicate higher inhibitory activities. Then, ACT and FCT were subjected to peptide sequencing using LC-MS-QTOF to identify the potential AG and AA inhibitors. Molecular docking was performed on peptides with the highest number of hotspots and PeptideRanker score to study their interactions with AG and AA enzymes. Among the peptides identified, SPRRF, FE, and RR were predicted to be the most active peptides against AG, while AA inhibitors were predicted to be RPR, PPGIP, and LRP. Overall, hydrolysates prepared from lupin proteins using alcalase and flavourzyme may be useful in formulating functional food for managing diabetics.
Publisher: Elsevier BV
Date: 2020
DOI: 10.1016/J.FOODCHEM.2019.125387
Abstract: In the study, the impact of harvest time on total phenolic content, antioxidant activity, and phenolic compounds of prickly pear (Opuntia ficus-barbarica A. Berger) fruit pulp and the oil content and fatty acids profile of the seed were investigated. The highest total phenolic content was determined as 156.77 mg/100 g in July 1 harvest, while the maximum antioxidant activity and total oil content were found as 9.81% and 6.80% at the last stage of maturation (15 August), respectively. The highest oleic (28.51%), palmitic (22.61%) and stearic acid contents (9.20%) in seed oil were observed in June 15 harvest. The highest value for linoleic acid (57.50%) was detected in August 15 harvest. Prickly pear is a vital source of bioactive constituents such as phenolic and antioxidant substances in terms of being useful for human health and the optimum harvesting time to retain high quantities of most phenolic compounds is 1st July.
Publisher: MDPI AG
Date: 21-06-2022
Abstract: Bioactive peptides (BPs) derived from animal and plant proteins are important food functional ingredients with many promising health-promoting properties. In the food industry, enzymatic hydrolysis is the most common technique employed for the liberation of BPs from proteins in which conventional heat treatment is used as pre-treatment to enhance hydrolytic action. In recent years, application of non-thermal food processing technologies such as ultrasound (US), high-pressure processing (HPP), and pulsed electric field (PEF) as pre-treatment methods has gained considerable research attention owing to the enhancement in yield and bioactivity of resulting peptides. This review provides an overview of bioactivities of peptides obtained from animal and plant proteins and an insight into the impact of US, HPP, and PEF as non-thermal treatment prior to enzymolysis on the generation of food-derived BPs and resulting bioactivities. US, HPP, and PEF were reported to improve antioxidant, angiotensin-converting enzyme (ACE)-inhibitory, antimicrobial, and antidiabetic properties of the food-derived BPs. The primary modes of action are due to conformational changes of food proteins caused by US, HPP, and PEF, improving the susceptibility of proteins to protease cleavage and subsequent proteolysis. However, the use of other non-thermal techniques such as cold plasma, radiofrequency electric field, dense phase carbon dioxide, and oscillating magnetic fields has not been examined in the generation of BPs from food proteins.
Publisher: Hindawi Limited
Date: 10-2018
DOI: 10.1111/JFPP.13710
Publisher: Springer Science and Business Media LLC
Date: 20-10-2018
Publisher: Wiley
Date: 17-01-2022
DOI: 10.1111/IJFS.15549
Abstract: This study examined how application of ultrasound pretreatment (UP) (400 W, 20 kHz for 5, 10 min) influenced the structural characteristics, molecular weight distribution, amino acid composition and biological activity of hydrolysate prepared from lupin protein using protamex enzyme. Circular dichroism (CD) and Fourier transform infrared (FTIR) techniques revealed changes in secondary structure after UP, while changes in molecular weight pattern were revealed by SDS‐PAGE and MALDI‐TOF analyses. Also, the crystallinity and surface hydrophobicity of the hydrolysates were affected by UP. The amino acid compositions of the hydrolysate varied, especially glutamic acid, arginine and aspartic acid. In vitro biological assays showed that antioxidant, α‐glucosidase, α‐amylase and ACE inhibitory activities significantly improved in sonicated hydrolysates compared with non‐sonicated s les, and this may be linked to the structural modifications caused by UP. Hence, the application of UP to lupin protein could en hance the biological activity of its hydrolysate.
Publisher: Elsevier BV
Date: 04-2021
Publisher: Elsevier BV
Date: 2004
Publisher: Walter de Gruyter GmbH
Date: 06-2019
Abstract: The objective of the present work was to investigate the influence of locations on bioactive propertiest, phenolic compounds and mineral contents of bee pollens. The oil content of pollen grains changed between 3.50% (Alanya) and 6.85% (Russia-Perm Region). The highest total phenolic content (720 mg/100g) and antioxidant activity values (81.4%) were observed in pollens obtained from the Russia-Perm Region and Alanya districts, respectively. Additionally, the highest carotenoid was found in a pollen s le collected from Karaman (Sarıveliler) (98.6 mg/g). The major phenolic compounds were (+)-catechin (66.75-337.39 mg/100g) and quercetin (61.2-1221.7 mg/100g) in all pollen s les. The pollen s les were observed to be a significant source of potassium (3846-6287 mg/kg), phosphorus (2947-5010 mg/kg), calcium (1022-2424 mg/kg) and sulfur (1744-2397 mg/kg). All of the analysis results were significantly affected by supplying locations. The antioxidant activity values of pollens were found partly similar and varied depending on locations. The content of saturated fatty acid (palmitic) was high (20-30%) in the tested pollen s les but did not exceed the content of linoleic acid.
Publisher: Springer Science and Business Media LLC
Date: 24-02-2020
Publisher: Wiley
Date: 12-11-2017
DOI: 10.1002/FSN3.539
Publisher: Elsevier BV
Date: 05-2023
Publisher: Springer Science and Business Media LLC
Date: 10-09-2021
Publisher: Springer Science and Business Media LLC
Date: 26-10-2020
Publisher: Editorial CSIC
Date: 19-02-2019
DOI: 10.3989/GYA.0933182
Abstract: This study investigated the free fatty acids, fatty acid profile, total phenolics, and antioxidant activity of sesame seed oil extracted from γ-irradiated seeds and the decontamination effects of the treatment on fungal incidence in the seeds. Gamma irradiation reduced (P ≤ 0.05) fungal growth and colony forming units of sesame seeds in a dose-dependent manner. The free fatty acid content of sesame oil decreased (P ≤ 0.05) in irradiated s les compared to non-radiated controls, but there was no difference (P ≥ 0.05) between s les treated at doses ≥ 1.0 kGy. A concomitant (P ≤ 0.05) increase in total phenolic and scavenging activity was observed in the oil extracted from γ-irradiated sesame seeds in comparison with non-radiated s les, while free fatty acid (FFA) content decreased. The results obtained in the present study demonstrate that γ-irradiation at low doses can be used as an effective post-harvest preservation method for sesame seeds without a major effect on the quality of sesame oil.
Publisher: Elsevier BV
Date: 07-2022
DOI: 10.1016/J.FOODCHEM.2022.132457
Abstract: This study evaluated the impact of ultrasound pretreatment (UP) and enzyme type (flavourzyme, alcalase) on amino acid composition, antihypertensive, antidiabetic, and antioxidant activities of lupin protein hydrolysate (LPH). The UP altered molecular weight of the hydrolysates as revealed by SDS-PAGE and MALDI-TOF and their surface hydrophobicity and thermal stability. Amino acid analysis revealed that glutamic acid, aspartic acid, arginine, and leucine were the dominant amino acids lupin protein. Generally, sonicated hydrolysates exhibited higher biological activity than non-sonicated s les and the original LPI. Alcalase hydrolysate pretreated for 10 min showed the highest DPPH and metal chelating activities. Highest ACE inhibitory activity was observed in flavourzyme hydrolysate after 5 min of UP, while maximum α-amylase and glucosidase inhibitory activity was achieved after 10 min of UP in flavourzyme hydrolysate. The results obtained in this study demonstrated the potentials of UP as a valuable tool for enhancing the biological activity of lupin proteins.
Publisher: Codon Publications
Date: 13-12-2018
DOI: 10.3920/QAS2018.1352
No related grants have been discovered for Gbemisola Fadimu.