ORCID Profile
0000-0002-9780-8844
Current Organisation
University of New South Wales (UNSW Sydney)
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Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Biomaterials | Nanotechnology | Nanomanufacturing | Nanomaterials | Medical Devices | Chemical Engineering not elsewhere classified | Sensor Technology (Chemical aspects) | Biomedical Engineering | Microtechnology | Chemical Engineering | Biomedical Engineering not elsewhere classified | Regenerative Medicine (incl. Stem Cells and Tissue Engineering) | Nanobiotechnology |
ANZSRC Socio-Economic Objective (SEO)
Diagnostic Methods | Expanding Knowledge in the Medical and Health Sciences | Skeletal System and Disorders (incl. Arthritis) | Medical Instruments | Expanding Knowledge in the Physical Sciences | Expanding Knowledge in Engineering | Human Pharmaceutical Products not elsewhere classified | Food Safety | Expanding Knowledge in Technology
Related Links
Publications
Zinc oxide particles release nitric oxide from endogenous and exogenous nitric oxide prodrugs
Publisher: American Chemical Society (ACS)
Date: 07-04-2020
Digital analysis of polydiacetylene quality tags for contactless monitoring of milk
Publisher: Elsevier BV
Date: 03-2021
Bloch surface wave enhanced biosensor for the direct detection of Angiopoietin-2 tumor biomarker in human plasma
Publisher: The Optical Society
Date: 08-01-2018
DOI: 10.1364/BOE.9.000529
Label-free and fluorescence biosensing platform using one dimensional photonic crystal chips
Publisher: SPIE
Date: 29-02-2016
DOI: 10.1117/12.2218517
Multicompartmentalized Microreactors Containing Nuclei and Catalase-Loaded Liposomes.
Publisher: American Chemical Society (ACS)
Date: 19-09-2018
DOI: 10.1021/ACS.BIOMAC.8B01260
Abstract: Multicompartmentalized microreactors are considered as cell mimics with hierarchical structures inspired by mammalian cells. We report the successful assembly and encapsulation of purified nuclei from RAW 264.7 cells (pNuc) into alginate-based microreactors. We demonstrate the preserved function of nuclei within the microreactors for mRNA production. Further, we load catalase-loaded liposomes (Lcat) into the microreactors to break down hydrogen peroxide (H
Polydiacetylene-based sensors to detect food spoilage at low temperatures
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C8TC05534C
Abstract: Polydiacetylene-based ammonia sensors as an indicator for meat spoilage at a wide range of food storage temperatures (RT, 4 °C, and −20 °C).
Cholesterol-mediated anchoring of enzyme-loaded liposomes within disulfide-stabilized polymer carrier capsules
Publisher: Elsevier BV
Date: 10-2009
DOI: 10.1016/J.BIOMATERIALS.2009.07.040
Abstract: Polymer capsules containing multiple liposomes, termed capsosomes, are a promising new concept toward the design of artificial cells. Herein, we report on the fundamental aspects underpinning the assembly of capsosomes. A stable and high loading of intact liposomal cargo into a polymer film was achieved by non-covalently sandwiching the liposomes between a tailor-made cholesterol-modified poly(L-lysine) (PLL(c)) precursor layer and a poly(methacrylic acid)-co-(cholesteryl methacrylate) (PMA(c)) capping layer. The film assembly, optimized on planar surfaces, was successfully transferred onto colloidal substrates, and a polymer membrane was subsequently assembled by the alternating adsorption of poly(N-vinyl pyrrolidone) (PVP) and thiol-modified poly(methacrylic acid) (PMA(SH)) onto the pre-adsorbed layer of liposomes. Upon removal of the silica template, stable capsosomes encapsulating the enzyme luciferase or beta-lactamase within their liposomal sub-compartments were obtained at both assembly (pH 4) and physiological conditions (pH 7.4). Excellent retention of the liposomes and the enzymatic cargo within the polymer carrier capsules was observed for up to 14 days. These engineered capsosomes are particularly attractive as autonomous microreactors, which can be utilized to repetitively add smaller reactants to cause successive distinct reactions within the capsosomes and simultaneously release the products to the surrounding environment, bringing these systems one step closer toward constructing artificial cells.
Capsosomes with Free-Floating Liposomal Subcompartments
Publisher: Wiley
Date: 27-07-2011
Polydiacetylene-based sensors for food applications
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D1MA01180D
Abstract: In this review, we discuss methods of polydiacetylene sensor fabrication, and their use as food quality and food contaminant sensors. Our perspective on the challenges and opportunities for polydiacetylene-based food sensors is included.
Artificial Antigen Presenting Cells for Detection and Desensitization of Autoreactive T cells Associated with Type 1 Diabetes
Publisher: American Chemical Society (ACS)
Date: 26-05-2022
Anthocyanin-based sensors derived from food waste as an active use-by date indicator for milk
Publisher: Elsevier BV
Date: 10-2020
Nitric Oxide to Fight Viral Infections
Publisher: Wiley
Date: 09-02-2021
Magnetic biosensors: Modelling and simulation
Publisher: Elsevier BV
Date: 04-2018
DOI: 10.1016/J.BIOS.2017.12.023
Abstract: In the past few years, magnetoelectronics has emerged as a promising new platform technology in various biosensors for detection, identification, localisation and manipulation of a wide spectrum of biological, physical and chemical agents. The methods are based on the exposure of the magnetic field of a magnetically labelled biomolecule interacting with a complementary biomolecule bound to a magnetic field sensor. This Review presents various schemes of magnetic biosensor techniques from both simulation and modelling as well as analytical and numerical analysis points of view, and the performance variations under magnetic fields at steady and nonstationary states. This is followed by magnetic sensors modelling and simulations using advanced Multiphysics modelling software (e.g. Finite Element Method (FEM) etc.) and home-made developed tools. Furthermore, outlook and future directions of modelling and simulations of magnetic biosensors in different technologies and materials are critically discussed.
Enzyme Prodrug Therapy Achieves Site-Specific, Personalized Physiological Responses to the Locally Produced Nitric Oxide
Publisher: American Chemical Society (ACS)
Date: 23-03-2018
Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C8NA00331A
Abstract: We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay.
Nanoassembled Peptide Biosensors for Rapid Detection of Matrilysin Cancer Biomarker
Publisher: Wiley
Date: 20-03-2020
Nanoparticle-based colorimetric sensors to detect neurodegenerative disease biomarkers
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1BM01226F
Abstract: We provide an overview of recent advances in colorimetric sensors to detect neurodegenerative disease biomarkers. Sensing strategies, limitations, and challenges are discussed, and an outlook on the future of this field is included.
Colloidal nanoparticles as advanced biological sensors
Publisher: American Association for the Advancement of Science (AAAS)
Date: 03-10-2014
Abstract: Colloidal fluorescent and plasmonic nanoparticles yield intense responses to incident light, making them useful as sensors or probes for sensitive detection in solution. Howes et al. review the potential uses of nanoparticle biosensors in research and diagnostics. A range of methods allow for the chemical modification of the particle surfaces so that they can be tuned for specific analytes and give optical signals for a range of biological conditions of interest. Signals can be detected in complex media or in vivo making the particles of interest for both laboratory research and in clinical settings. Science , this issue 10.1126/science.1247390
Layer-by-Layer Self-Assembly of Polymer Films and Capsules through Coiled-Coil Peptides
Publisher: American Chemical Society (ACS)
Date: 13-03-2026
A Polydiacetylene‐Based Colorimetric Sensor as an Active Use‐By Date for Plant‐Based Milk Alternatives
Publisher: Wiley
Date: 27-05-2020
Engineering Extracellular Vesicles with the Tools of Enzyme Prodrug Therapy
Publisher: Wiley
Date: 23-02-2018
Polydiacetylene-based sensors to detect food spoilage
Publisher: American Chemical Society (ACS)
Date: 07-04-2020
Metal-organic frameworks for therapeutic gas delivery
Publisher: Elsevier BV
Date: 04-2021
Enzyme Prodrug Therapy Engineered into Electrospun Fibers with Embedded Liposomes for Controlled, Localized Synthesis of Therapeutics
Publisher: Wiley
Date: 12-07-2017
Engineering Advanced Capsosomes: Maximizing the Number of Subcompartments, Cargo Retention, and Temperature-Triggered Reaction
Publisher: American Chemical Society (ACS)
Date: 03-2010
DOI: 10.1021/NN901843J
Abstract: Advanced mimics of cells require a large yet controllable number of subcompartments encapsulated within a scaffold, equipped with a trigger to initiate, terminate, and potentially restart an enzymatic reaction. Recently introduced capsosomes, polymer capsules containing thousands of liposomes, are a promising platform for the creation of artificial cells. Capsosomes are formed by sequentially layering liposomes and polymers onto particle templates, followed by removal of the template cores. Herein, we engineer advanced capsosomes and demonstrate the ability to control the number of subcompartments and hence the degree of cargo loading. To achieve this, we employ a range of polymer separation layers and liposomes to form functional capsosomes comprising multiple layers of enzyme-loaded liposomes. Differences in conversion rates of an enzymatic assay are used to verify that multilayers of intact enzyme-loaded liposomes are assembled within a polymer hydrogel capsule. The size-dependent retention of the cargo encapsulated within the liposomal subcompartments during capsosome assembly and its dependence on environmental pH changes are also examined. We further show that temperature can be used to trigger an enzymatic reaction at the phase transition temperature of the liposomal subcompartments, and that the encapsulated enzymes can be utilized repeatedly in several subsequent conversions. These engineered capsosomes with tailored properties present new opportunities en route to the development of functional artificial cells.
Enzyme-responsive polymer hydrogels for therapeutic delivery
Publisher: SAGE Publications
Date: 27-04-2016
Abstract: Enzymes play a central role in a spectrum of fundamental physiological processes and their altered expression level has been associated with many diseases and pathological disorders. Enzymes therefore can be exploited as a pristine biological trigger to tune material responses and to achieve controlled release of biomolecules at desired sites. This mini-review highlights enzyme-responsive polymer hydrogels for therapeutic delivery applications developed within the last five years, focusing on protease- and glycosidase-based catalyzed reactions. Strategies employed to produce responsive materials are described. Successful applications for controlled drug delivery are highlighted, and finally, future opportunities and challenges are presented.
Polymer materials as catalysts for medical, environmental, and energy applications
Publisher: Elsevier BV
Date: 12-2023
Sensitivity and Selectivity Analysis of Fluorescent Probes for Hydrogen Sulfide Detection
Publisher: Wiley
Date: 27-01-2022
Abstract: Hydrogen sulfide (H 2 S) is a gasotransmitter known to regulate physiological and pathological processes. Abnormal H 2 S levels have been associated with a range of conditions, including Parkinson's and Alzheimer's diseases, cardiovascular and renal diseases, bacterial and viral infections, as well as cancer. Therefore, fast and sensitive H 2 S detection is of significant clinical importance. Fluorescent H 2 S probes hold great potential among the currently developed detection methods because of their high sensitivity, selectivity, and biocompatibility. However, many proposed probes do not provide a gold standard for proper use and selection. Consequently, issues arise when applying the probes in different conditions. Therefore, we systematically evaluated four commercially available probes (WSP‐1, WSP‐5, CAY, and P3), considering their detection range, sensitivity, selectivity, and performance in different environments. Furthermore, their capacity for endogenous H 2 S imaging in live cells was demonstrated.
Food Sensors: Challenges and Opportunities
Publisher: Wiley
Date: 14-03-2021
Core-satellite gold nanoparticle biosensors for monitoring cobalt ions in biological samples
Publisher: Elsevier BV
Date: 09-2018
Nanozyme-based sensors for detection of food biomarkers: a review
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D2RA04444G
Abstract: We present a discussion on different types of sensors used in food biomarker detection and highlight recent studies on nanozyme-based sensors to detect markers like toxins, pathogens, antibiotics, growth hormones, metal ions, additives, small molecules, drug residues.
Degradation of liposomal subcompartments in PEGylated capsosomes
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C1SM05623A
Tuning crystallization and morphology of zinc oxide with polyvinylpyrrolidone: Formation mechanisms and antimicrobial activity
Publisher: Elsevier BV
Date: 06-2019
DOI: 10.1016/J.JCIS.2019.03.051
Abstract: Zinc oxide (ZnO) particles with different shapes and sizes have been previously reported to possess unique optical, electrical, photocatalytic, and antimicrobial properties. Capping agents are routinely used to control particle morphologies however, few studies have evaluated the influence of capping agents on the growth kinetics of ZnO particles of different shapes. Herein, we report a simple water-based chemical precipitation method to produce unique bowtie-, flower-, and nest-shaped ZnO particles using zinc nitrate and urea in the presence of polyvinylpyrrolidone (PVP). Three distinct particle morphologies are obtained by adjusting polymer concentration during synthesis. This approach is simple and could enable large-scale production of ZnO particles with erse shapes. We monitor the morphological evolution of ZnO particles and, at different polymer concentrations, uncover the preferable PVP adsorption onto different ZnO facets that controls the growth directions of ZnO. Previous reports have demonstrated the influence of particle shape on ZnO antibacterial activity. In this study, we show that ZnO particles with these three morphologies exhibit similar bacterial killing efficacy towards Escherichia coli and Staphylococcus aureus. Our detailed mechanistic studies suggest that the antibacterial mechanism of ZnO particles can be attributed to both Zn
Progress and Promise of Nitric Oxide-Releasing Platforms
Publisher: Wiley
Date: 23-04-2018
Abstract: Nitric oxide (NO) is a highly potent radical with a wide spectrum of physiological activities. Depending on the concentration, it can enhance endothelial cell proliferation in a growth factor‐free medium, mediate angiogenesis, accelerate wound healing, but may also lead to tumor progression or induce inflammation. Due to its multifaceted role, NO must be administered at a right dose and at the specific site. Many efforts have focused on developing NO‐releasing biomaterials however, NO short half‐life in human tissues only allows this molecule to diffuse over short distances, and significant challenges remain before the full potential of NO can be realized. Here, an overview of platforms that are engineered to release NO via catalytic or noncatalytic approaches is presented, with a specific emphasis on progress reported in the past five years. A number of NO donors, natural enzymes, and enzyme mimics are highlighted, and recent promising developments of NO‐releasing scaffolds, particles, and films are presented. In particular, key parameters of NO delivery are discussed: 1) NO payload, 2) maximum NO flux, 3) NO release half‐life, 4) time required to reach maximum flux, and 5) duration of NO release. Advantages and drawbacks are reviewed, and possible further developments are suggested.
Solvent injection for polydiacetylene particle synthesis – Effects of varying solvent, injection rate, monomers and needle size on polydiacetylene properties
Publisher: Elsevier BV
Date: 06-2021
Artificial Antigen Presenting Cells for Detection and Desensitisation of Auto-reactive T cells Associated with Type 1 Diabetes
Publisher: Cold Spring Harbor Laboratory
Date: 27-12-2020
DOI: 10.1101/2020.12.26.424333
Abstract: Autoimmune diseases and in particular type 1 diabetes rely heavily on treatments that target the symptoms rather than prevent the underlying disease. One of the barriers to better therapeutic strategies is the inability to detect and efficiently target rare autoreactive T-cell populations that are major drivers of these conditions. Here, we develop a unique artificial antigen presenting cell (aAPC) system from biocompatible polymer particles that allows specific encapsulation of bioactive ingredients. Using our aAPC we demonstrate that we are able to detect rare autoreactive CD4 populations in human patients and using mouse models we demonstrate that our particles are able to induce desensitization in the autoreactive population. This system provides a promising tool that can be used in the prevention of autoimmunity before disease onset.
Wearable Platform for Low‐Dose Inhaled Nitric Oxide Therapy
Publisher: Wiley
Date: 29-01-2023
Abstract: The ongoing COVID‐19 pandemic has been a daunting challenge for healthcare systems worldwide. The World Health Organization has recommended various measures to reduce or limit the spread of the virus, one of which includes the use of face masks. This increase in their demand has provided a unique opportunity to improve the technology by offering, in addition to their inherent protection, therapeutic benefits. One such benefit involves inhaled nitric oxide (iNO) therapy. iNO has proven to be a beneficial therapeutic in patients with acute, hypoxemic respiratory failure and lung injury. Specifically, its potential application stems from its ability to rapidly increase oxygen partial pressure in arterial blood. However, iNO treatments generally require pressurized gas cylinders which are coupled with high costs and lack of portability. A face mask which can deliver therapeutic NO is developed using humidity‐triggered NO‐releasing nanoparticles. This platform can deliver a low dose of 2.1–2.5 ppm NO for 90 min in a sustained manner. Moreover, it can be stored for extended periods of time and can be easily transported due to its light weight. This NO mask has the potential to alleviate the strain that affects financially limited healthcare systems in developing regions.
Peptide-Induced Super-Assembly of Biocatalytic Metal-Organic Frameworks for Programmed Enzyme Cascade
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9SC02021G
Abstract: Complementary peptide-induced super-assembly of biocatalytic MOFs is developed for programmed enzyme cascades on demand.
A polydiacetylene-based colorimetric sensor as an active use-by date indicator for milk
Publisher: Elsevier BV
Date: 07-2020
Noncovalent Liposome Linkage and Miniaturization of Capsosomes for Drug Delivery
Publisher: American Chemical Society (ACS)
Date: 19-11-2010
DOI: 10.1021/BM101020E
Abstract: We report the synthesis of poly(methacrylic acid)-co-(oleyl methacrylate) with three different amounts of oleyl methacrylate and compare the ability of these polymers with that of poly(methacrylic acid)-co-(cholesteryl methacrylate) (PMA(c)) to noncovalently anchor liposomes to polymer layers. We subsequently assembled ∼1 μm diameter PMA(c)-based capsosomes, polymer hydrogel capsules that contain up to ∼2000 liposomal subcompartments, and investigate the potential of these carriers to deliver water-insoluble drugs by encapsulating two different antitumor compounds, thiocoraline or paclitaxel, into the liposomes. The viability of lung cancer cells is used to substantiate the cargo concentration-dependent activity of the capsosomes. These findings cover several crucial aspects for the application of capsosomes as potential drug delivery vehicles.
Paper-based sensors for bacteria detection
Publisher: Springer Science and Business Media LLC
Date: 14-02-2023
Ceria Nanoparticles as an Unexpected Catalyst to Generate Nitric Oxide from S ‐Nitrosoglutathione
Publisher: Wiley
Date: 20-01-2022
Abstract: Ceria nanoparticles (NPs) are widely reported to scavenge nitric oxide (NO) radicals. This study reveals evidence that an opposite effect of ceria NPs exists, that is, to induce NO generation. Herein, S ‐nitrosoglutathione (GSNO), one of the most biologically abundant NO donors, is catalytically decomposed by ceria NPs to produce NO. Ceria NPs maintain a high NO release recovery rate and retain their crystalline structure for at least 4 weeks. Importantly, the mechanism of this newly discovered NO generation capability of ceria NPs from GSNO is deciphered to be attributed to the oxidation of Ce 3+ to Ce 4+ on their surface, which is supported by X‐ray photoelectron spectroscopy and density functional theory analysis. The prospective therapeutic effect of NO‐generating ceria NPs is evaluated by the suppression of cancer cells, displaying a significant reduction of 93% in cell viability. Overall, this report is, to the authors’ knowledge, the first study to identify the capability of ceria NPs to induce NO generation from GSNO, which overturns the conventional concept of them acting solely as a NO‐scavenging agent. This study will deepen our knowledge about the therapeutic effects of ceria NPs and open a new route toward the NO‐generating systems for biomedical applications.
Biomimetic Liposome- and Polymersome-Based Multicompartmentalized Assemblies
Publisher: American Chemical Society (ACS)
Date: 25-07-2012
DOI: 10.1021/LA301958V
Abstract: Liposomes and polymersomes have attracted significant attention and have emerged as versatile materials for therapeutic delivery and in the design of artificial cells and organelles. Through the judicious choice of building blocks, these synthetic carriers can be readily engineered with tailored interfacial properties, offering new possibilities for the design of advanced assemblies with specific permeability, stability, stimuli response, and targeting capabilities. In this feature article, we highlight recent studies on biomimetic liposome- and polymersome-based multicompartmentalized assemblies en route toward the development of artificial cells, microreactors, and therapeutic delivery carriers. The strategies employed to produce these carriers are outlined, and the properties that contribute to their performance are discussed. Applications of these biomimetic assemblies are highlighted, and finally, areas that require additional investigation for the future development of these assemblies as next-generation therapeutic systems are outlined.
Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices
Publisher: Elsevier BV
Date: 02-2018
Label-free detection of angiogenesis biomarkers using Bloch surface waves on one dimensional photonic crystals
Publisher: IEEE
Date: 07-2015
Engineering receptor-mediated transmembrane signaling in artificial and living cells
Publisher: Springer Science and Business Media LLC
Date: 28-08-2023
DOI: 10.1038/S43246-023-00394-Z
Abstract: Living cells possess a variety of transmembrane signaling systems that receive chemical and physical cues from the environment and transduce this information into an intracellular signal that triggers downstream cellular responses. This Review aims to present recent advances in the design of bioinspired systems that mimic transmembrane signaling in synthetic and living cells. We summarize the building of cell-like membranous structures and the construction of interfacial recognition between chemical or biological components. After introducing two main mechanisms of signal transduction in biology through receptor tyrosine kinase and G‐protein coupled receptors, we then highlight the possibility of mimicking transmembrane signaling with transducer integrated systems. We survey the potential for applying supramolecular materials (e.g., DNA origami, polypeptide structures and polymer scaffolds) to interact with surface receptors on the plasma membrane, providing an avenue for the application of therapeutics.
Controlled assembly of peptide-functionalized gold nanoparticles for label-free detection of blood coagulation Factor XIII activity
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4CC00572D
Abstract: A highly sensitive label-free assay for the determination of blood coagulation Factor XIII activity is demonstrated through the controlled assembly of peptide-functionalized gold nanoparticles (AuNPs). Activated Factor XIII catalyzes the formation of covalent crosslinking between peptide chains through ε-(γ-glutamyl)-lysine bonds leading to the aggregation of the AuNPs and consequently a red-shift of the localized surface plasmon resonance. The selective engineering of nanoscale order over AuNP crosslinking via the formation of isopeptide bonds provides a new approach toward the design of nanoassemblies with precise control on the molecular level. The colorimetric assay reported here provides direct qualitative and quantitative analysis of Factor XIII activity with a limit of detection of 0.01 U mL(-1).
Therapeutic applications of multifunctional nanozymes
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9NR06596B
Abstract: This review summarizes novel applications of multifunctional nanozymes in various biomedical-related fields ranging from cancer diagnosis, cancer and antibacterial therapy to regenerative medicine.
Liposomes and lipid bilayers in biosensors.
Publisher: Elsevier BV
Date: 11-2017
DOI: 10.1016/J.CIS.2017.05.020
Abstract: Biosensors for the rapid, specific, and sensitive detection of analytes play a vital role in healthcare, drug discovery, food safety, and environmental monitoring. Although a number of sensing concepts and devices have been developed, many longstanding challenges to obtain inexpensive, easy-to-use, and reliable sensor platforms remain largely unmet. Nanomaterials offer exciting possibilities for enhancing the assay sensitivity and for lowering the detection limits down to single-molecule resolution. In this review, we present an overview of liposomes and lipid bilayers in biosensing applications. Lipid assemblies in the form of spherical liposomes or two-dimensional planar membranes have been widely used in the design of biosensing assays in particular, we highlight a number of recent promising developments of biosensors based on liposomes in suspension, liposome arrays, and lipid bilayers arrays. Assay sensitivity and specificity are discussed, advantages and drawbacks are reviewed, and possible further developments are outlined.
Nanocellulose for Sensing Applications
Publisher: Wiley
Date: 09-08-2019
Abstract: Nanocellulose market is estimated to be worth $660 million by 2023. Rapid advances in nanotechnology and material sciences have facilitated investigation of nanocellulose, giving rise to their emergence as desirable biomaterials. There has been substantial research undertaken on the applications of nanocellulose in various fields such as renewable energy, electronics, environment, food agriculture, biomedical, and healthcare. More recent attention has focused on the applications of nanocellulose in sensor domain. Here, the recent developments of nanocellulose with more focus on cellulose nanocrystals in sensing applications are highlighted and discussed in detail.
Multicompartment Particle Assemblies for Bioinspired Encapsulated Reactions
Publisher: American Chemical Society (ACS)
Date: 06-10-2011
DOI: 10.1021/JZ200994N
Synthetic chemical ligands and cognate antibodies for biorthogonal drug targeting and cell engineering
Publisher: Elsevier BV
Date: 03-2021
Polydiacetylene-Based Sensors To Detect Volatile Organic Compounds
Publisher: American Chemical Society (ACS)
Date: 21-03-2022
Low-fouling, biospecific films prepared by the continuous assembly of polymers
Publisher: American Chemical Society (ACS)
Date: 10-07-2013
DOI: 10.1021/BM400680D
Abstract: We report that the continuous assembly of polymers (CAP) approach, mediated by ring-opening metathesis polymerization (ROMP), is a facile and versatile technology to prepare engineered nanocoatings for various biomedical applications. Low-fouling coatings on particles were obtained by the formation of multicompositional, layered films via simple and efficient tandem CAP(ROMP) processes that are analogous to chain extension reactions. In addition, the CAP(ROMP) approach allows for the efficient postfunctionalization of the CAP films with bioactive moieties via cross-metathesis reactions between the surface-immobilized catalysts and symmetrical alkene derivatives. The combined features of the CAP(ROMP) approach (i.e., versatile polymer selection and facile functionalization) allow for the fabrication and surface modification of various types of polymer films, including those with intrinsic protein-repellent properties and selective protein recognition capabilities. This study highlights the various types of advanced coatings and materials that the CAP approach can be used to generate, which may be useful for biomedical applications.
Peptide‐Mediated Liposome Fusion as a Tool for the Detection of Matrix Metalloproteinases
Publisher: Wiley
Date: 12-03-2019
Naked-Eye Detection of Ethylene Using Thiol-Functionalized Polydiacetylene-Based Flexible Sensors
Publisher: American Chemical Society (ACS)
Date: 18-06-2020
Zinc Oxide Particles Catalytically Generate Nitric Oxide from Endogenous and Exogenous Prodrugs
Publisher: Wiley
Date: 05-03-2020
Enzyme Mimics for the Catalytic Generation of Nitric Oxide from Endogenous Prodrugs
Publisher: Wiley
Date: 05-05-2020
Biennial State-of-the-Art Sensors Technology in Australia 2019-2020
Publisher: MDPI
Date: 27-04-2021
Modulating nitric oxide-generating activity of zinc oxide by morphology control and surface modification
Publisher: Elsevier BV
Date: 11-1970
Gallium Nanodroplets are Anti-Inflammatory without Interfering with Iron Homeostasis
Publisher: American Chemical Society (ACS)
Date: 25-05-2022
Abstract: Gallium (Ga) compounds, as the source of Ga ions (Ga
MicroRNA Detection by DNA‐Mediated Liposome Fusion
Publisher: Wiley
Date: 15-01-2018
3D printing of biopolymer nanocomposites for tissue engineering: Nanomaterials, processing and structure-function relation
Publisher: Elsevier BV
Date: 12-2019
Synthetic nanoprobes for biological hydrogen sulfide detection and imaging
Publisher: Wiley
Date: 18-02-2022
DOI: 10.1002/VIW.20210008
Abstract: Hydrogen sulfide (H 2 S) is a gaseous molecule involved in multiple biological and physiological processes, including various diseases such as cancer and neurodegenerative disorders. This has led to the development of various analytical methods to monitor H 2 S in biological settings. Among these, fluorescence‐based assays, specifically organic small‐molecule probes, have been thoroughly utilized. They offer good sensitivity and specificity as sensors, and noninvasive detection with high spatiotemporal resolution in in vitro and in vivo imaging. Despite attempts to decrease the rate of photobleaching and enhance the photostability of these dyes, they are still limited by low survival time and complex reagent pretreatment. Fortunately, nanotechnology has been applied to develop effective, highly sensitive, and specific fluorescent nanoprobes. Specifically, nanomaterial‐based H 2 S probes have emerged as promising candidates for real‐time detection and imaging. In contrast to their organic molecule‐based counterparts, they offer higher versatility in imaging modes due to their unique optical properties, improved photostability and solubility within physiological fluids, as well as easily modifiable surfaces and tuneable structures for improved specificity. Recently, many nanomaterial‐based probes, ranging from inorganic nanoparticles to self‐assembled nanocomposites, have been developed. These have, for the most part, achieved sensitive and specific endogenous H 2 S detection and in vivo imaging. In this review, we evaluate five different nanomaterials currently being researched to detect and image endogenous H 2 S within the last 5 years. Furthermore, analytical methods associated with the various signal outputs, current challenges in H 2 S nanoprobe design, and possible future research interests are outlined and discussed.
Tuning chromatic response, sensitivity, and specificity of polydiacetylene-based sensors
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/C9PY00949C
Abstract: In this review, we provide an overview of six major techniques to tune the sensitivity and specificity of polydiacetylene-based sensors.
Trigit: A free web application for rapid colorimetric analysis of images
Publisher: Elsevier BV
Date: 09-2023
Capsosomes: Subcompartmentalizing Polyelectrolyte Capsules Using Liposomes
Publisher: American Chemical Society (ACS)
Date: 16-06-2009
DOI: 10.1021/LA900213A
Abstract: Next-generation therapeutic approaches are expected to rely on the engineering of multifunctional particle carriers that can mimic specific cellular functions. The key features of such particles are the semipermeable nature of the shell for communication with the external environment and multiple nanosized in idual subcompartments confined within a micron-sized structurally stable scaffold for conducting specific reactions. Herein, we report the formation of capsosomes, a new class of polyelectrolyte capsules containing structurally intact liposomes as cargo. The multilayer film assembly of polyelectrolytes (poly(styrene sulfonate) (PSS) and poly(allylamine hydrochloride) (PAH)) and liposomes (50 nm 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)) was characterized on planar substrates using quartz crystal microbalance with dissipation monitoring, and these findings were then correlated to the film growth of the polyelectrolytes and structurally intact liposomes on silica particles. Upon removal of the silica template core, stable capsosomes, containing one or two layers of intact liposomes as cargo, were obtained. This novel platform, capsosomes, which combines the advantages of two systems, liposomes and polyelectrolyte capsules, is expected to find erse applications in biomedicine, in particular for the creation of artificial cells or organelles where the performance of reactions within a confined environment is a prerequisite.
Metal and Metal Oxide Nanoparticles to Enhance the Performance of Enzyme-Linked Immunosorbent Assay (ELISA)
Publisher: American Chemical Society (ACS)
Date: 09-12-2019
A Microreactor with Thousands of Subcompartments: Enzyme-Loaded Liposomes within Polymer Capsules
Publisher: Wiley
Date: 26-05-2009
Abstract: Fully loaded: Noncovalent anchoring of liposomes into polymer multilayered films with cholesterol-modified polymers allows the preparation of capsosomes-liposome-compartmentalized polymer capsules (see picture). A quantitative enzymatic reaction confirmed the presence of active cargo within the capsosomes and was used to determine the number of subcompartments within this novel biomedical carrier system.
Polymer hydrogel capsules: en route toward synthetic cellular systems
Publisher: Royal Society of Chemistry (RSC)
Date: 2009
DOI: 10.1039/B9NR00143C
Abstract: Engineered synthetic cellular systems are expected to become a powerful biomedical platform for the development of next-generation therapeutic carrier vehicles. In this mini-review, we discuss the potential of polymer capsules derived by the layer-by-layer assembly as a platform system for the construction of artificial cells and organelles. We outline the characteristics of polymer capsules that make them unique for these applications, and we describe several successful ex les of microencapsulated catalysis, including biologically relevant enzymatic reactions. We also provide ex les of subcompartmentalized polymer capsules, which represent a major step toward the creation of synthetic cells.
Synthesis and self-assembly of temperature-responsive copolymers based on N-vinylpyrrolidone and triethylene glycol methacrylate
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5PY00483G
Abstract: Synthesis of well-defined block copolymers of PVP- co -pTEGMA from a dual RAFT/ATRP CTA and study of their temperature-responsive properties driving self-assembly.
Self-assembly and bioconjugation in drug delivery
Publisher: Elsevier BV
Date: 07-2021
Layer-by-Layer Engineered Polymer Capsules for Therapeutic Delivery
Publisher: Springer New York
Date: 2018
DOI: 10.1007/978-1-4939-7741-3_6
Abstract: Polymer capsules fabricated via layer-by-layer (LbL) assembly have emerged as promising carriers for therapeutic delivery. The versatile assembly technique allows an extensive choice of materials to be incorporated as constituents of the multilayers, which therefore endow capsules with specific properties and functionalities. This chapter describes protocols for fabrication of LbL-engineered poly(methacrylic acid) (PMA) capsules for applications in gene delivery, including (1) synthesis of building blocks, (2) cargo encapsulation, (3) multilayer film formation, (4) surface modification, and (5) cross-linking of multilayer films and dissolution of particle templates. DNA is adsorbed onto positively charged silica particle templates, followed by formation of polymer films via hydrogen-bonded multilayers of thiol-functionalized PMA and poly(N-vinylpyrrolidone) (PVP). The outer polymer membranes can be surface modified with copolymers of PMA and poly(ethylene glycol) (PEG). Upon film stabilization and dissolution of particle templates, disulfide-cross-linked DNA-loaded PMA capsules are obtained, which serve as therapeutic carriers that can degrade and facilitate cargo release in intracellular reducing environment.
Locomotion of Micromotors Due to Liposome Disintegration
Publisher: American Chemical Society (ACS)
Date: 25-02-2020
Fabrication of polydiacetylene particles using a solvent injection method
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0MA00442A
Abstract: This study presents the first application of the solvent injection method to synthesize polydiacetylene vesicles.
Copper-Doped Metal-Organic Frameworks for the Controlled Generation of Nitric Oxide from Endogenous S-nitrosothiols
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D0TB02709J
Abstract: We report the synthesis of a catalyst, copper-doped zeolitic imidazolate framework ZIF-8, that generates nitric oxide from naturally occurring endogenous nitric oxide donors, S -nitrosoglutathione and S -nitrosocysteine.
Selective etching of injection molded zirconia-toughened alumina: Towards osseointegrated and antibacterial ceramic implants
Publisher: Elsevier BV
Date: 12-2016
DOI: 10.1016/J.ACTBIO.2016.09.017
Abstract: Due to their outstanding mechanical properties and excellent biocompatibility, zirconia-toughened alumina (ZTA) ceramics have become the gold standard in orthopedics for the fabrication of ceramic bearing components over the last decade. However, ZTA is bioinert, which h ers its implantation in direct contact with bone. Furthermore, periprosthetic joint infections are now the leading cause of failure for joint arthroplasty prostheses. To address both issues, an improved surface design is required: a controlled micro- and nano-roughness can promote osseointegration and limit bacterial adhesion whereas surface porosity allows loading and delivery of antibacterial compounds. In this work, we developed an integrated strategy aiming to provide both osseointegrative and antibacterial properties to ZTA surfaces. The micro-topography was controlled by injection molding. Meanwhile a novel process involving the selective dissolution of zirconia (selective etching) was used to produce nano-roughness and interconnected nanoporosity. Potential utilization of the porosity for loading and delivery of antibiotic molecules was demonstrated, and the impact of selective etching on mechanical properties and hydrothermal stability was shown to be limited. The combination of injection molding and selective etching thus appears promising for fabricating a new generation of ZTA components implantable in direct contact with bone. Zirconia-toughened alumina (ZTA) is the current gold standard for the fabrication of orthopedic ceramic components. In the present work, we propose an innovative strategy to provide both osseointegrative and antibacterial properties to ZTA surfaces: we demonstrate that injection molding allows a flexible design of surface micro-topography and can be combined with selective etching, a novel process that induces nano-roughness and surface interconnected porosity without the need for coating, avoiding reliability issues. These surface modifications have the potential to improve osseointegration. Furthermore, our results show that the porosity can be used for drug delivery and suggest that the etched surface could reduce bacterial adhesion.
Membrane Fusion Models for Bioapplications
Publisher: Wiley
Date: 26-01-2021
Triggered Cargo Release by Encapsulated Enzymatic Catalysis in Capsosomes
Publisher: American Chemical Society (ACS)
Date: 12-10-2011
DOI: 10.1021/NL202906J
Abstract: We report the coencapsulation of glutathione reductase and disulfide-linked polymer-oligopeptide conjugates into capsosomes, polymer carrier capsules containing liposomal subcompartments. The architecture of the capsosomes enables a temperature-triggered conversion of oxidized glutathione to its reduced sulfhydryl form by the encapsulated glutathione reductase. The reduced glutathione subsequently induces the release of the encapsulated oligopeptides from the capsosomes by reducing the disulfide linkages of the conjugates. This study highlights the potential of capsosomes to continuously generate a potent antioxidant while simultaneously releasing small molecule therapeutics.
Multi-Stimuli-Responsive Polymer Particles, Films, and Hydrogels for Drug Delivery
Publisher: Elsevier BV
Date: 09-2018
Localized and Controlled Delivery of Nitric Oxide to the Conventional Outflow Pathway via Enzyme Biocatalysis: Toward Therapy for Glaucoma
Publisher: Wiley
Date: 21-02-2017
Capsosomes as Long-Term Delivery Vehicles for Protein Therapeutics
Publisher: American Chemical Society (ACS)
Date: 09-07-2015
DOI: 10.1021/ACS.LANGMUIR.5B01667
Abstract: We report the preparation of polymer capsules containing liposomal subcompartments, termed capsosomes, and their ability for the sustained delivery of protein therapeutics. Capsosomes were formed through the layer-by-layer (LbL) assembly of polymers and protein-loaded liposomes, followed by the formation of a capsule membrane based on disulfide cross-linked poly(methacrylic acid). The loading capacities of a model cargo (lysozyme) and brain-derived neurotrophic factor (BDNF), an important neurotrophin that has significant physiological functions on the nervous system, were determined, and the long-term release kinetics of the proteins was investigated in simulated physiological conditions. The capsosomes exhibited protein loading and release behavior that can be tuned by the lipid composition of the liposomal compartments, where inclusion of anionic lipids resulted in enhanced protein loading and slower release over the course of 80 days. These findings highlight the potential of capsosomes for the long-term delivery of protein therapeutics.
Polymeric Amines Induce Nitric Oxide Release from S‐Nitrosothiols
Publisher: Wiley
Date: 05-07-2022
Abstract: Catalytic generation of nitric oxide (NO) from NO donors by nanomaterials has enabled prolonged NO delivery for various biomedical applications, but this approach requires laborious synthesis routes. In this study, a new class of materials, that is, polymeric amines including polyethyleneimine (PEI), poly‐L‐lysine, and poly(allylamine hydrochloride), is discovered to induce NO generation from S ‐nitrosothiols (RSNOs) at physiological conditions. Controlled NO generation can be readily achieved by tuning the concentration of the NO donors (RSNOs) and polymers, and the type and molecular weight of the polymers. Importantly, the mechanism of NO generation by these polymers is deciphered to be attributed to the nucleophilic reaction between primary amines on polymers and the SNO groups of RSNOs. The NO‐releasing feature of the polymers can be integrated into a suite of materials, for ex le, simply by embedding PEI into poly(vinyl alcohol) (PVA) hydrogels. The functionality of the PVA/PEI hydrogels is demonstrated for Pseudomonas aeruginosa biofilm prevention with a ≈ 4 log reduction within 6 h. As NO has potential therapeutic implications in various diseases, the identification of polymeric amines to induce NO release will open new opportunities in NO‐generating biomaterials for antibacterial, antiviral, anticancer, antithrombotic, and wound healing applications.
Rapid Detection of Listeriolysin O Toxin Based on a Nanoscale Liposome–Gold Nanoparticle Platform
Publisher: American Chemical Society (ACS)
Date: 15-06-2020
Stabilization of Polymer-Hydrogel Capsules via Thiol-Disulfide Exchange
Publisher: Wiley
Date: 11-11-2009
Polydiacetylene for the Detection of α-Hemolysin in Milk toward the Diagnosis of Bovine Mastitis
Publisher: American Chemical Society (ACS)
Date: 18-09-2020
Related Organisations
University Of Oxford
Location: United Kingdom of Great Britain and Northern Ireland
Imperial College London
Location: United Kingdom of Great Britain and Northern Ireland
Related Funding Activities
Bioactive Coatings To Improve Function And Lifetime Of Implantable Medical Devices
Start Date: 2020
End Date: 2025
Funder: National Health and Medical Research Council Emerging Leadership Fellowship
View Funded ActivityIndustrial Transformation Training Centres - Grant ID: IC170100022
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End Date: 06-2022
Amount: $4,420,408.00
Funder: Australian Research Council
View Funded ActivityLinkage Infrastructure, Equipment And Facilities - Grant ID: LE210100156
Start Date: 2021
End Date: 12-2021
Amount: $289,500.00
Funder: Australian Research Council
View Funded ActivityDiscovery Early Career Researcher Award - Grant ID: DE170100068
Start Date: 04-2017
End Date: 03-2020
Amount: $390,000.00
Funder: Australian Research Council
View Funded ActivityLinkage Projects - Grant ID: LP160100988
Start Date: 06-2017
End Date: 12-2019
Amount: $430,000.00
Funder: Australian Research Council
View Funded Activity