ORCID Profile
0000-0002-5692-7898
Current Organisation
USDA Agricultural Research Service
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Publisher: American Society for Microbiology
Date: 06-2014
DOI: 10.1128/CVI.00775-13
Abstract: Johne's disease in ruminants is a chronic infection of the intestines caused by Mycobacterium avium subsp. paratuberculosis . An important strategy to control disease is early detection, and a potentially efficient method for early detection is measurement of cell-mediated immune responses developed by the host in response to exposure or infection. One method is to measure lymphoproliferation and cytokine release from the host cells when exposed to the organism or parts of the organism. In this study, 10 recombinant M. avium subsp. paratuberculosis proteins known to be upregulated under in vitro stress conditions were evaluated by examining their ability to evoke memory as a result of exposure by vaccination or oral challenge with live Mycobacterium avium subsp. paratuberculosis . Out of 10 proteins, MAP2698c was found to induce higher cell-mediated immune responses in vaccinated and challenged sheep in comparison to healthy controls. The findings suggest that not all stress-regulated proteins have the diagnostic potential to detect cell-mediated immune responses in ovine paratuberculosis.
Publisher: American Society for Microbiology
Date: 12-2013
DOI: 10.1128/CVI.00596-13
Abstract: Mycobacterium avium subsp. paratuberculosis causes Johne's disease (JD) in ruminants. Proteomic studies have shown that M. avium subsp. paratuberculosis expresses certain proteins when exposed to in vitro physiological stress conditions similar to the conditions experienced within a host during natural infection. Such proteins are hypothesized to be expressed in vivo , are recognized by the host immune system, and may be of potential use in the diagnosis of JD. In this study, 50 recombinant maltose binding protein (MBP)- M. avium subsp. paratuberculosis fusion proteins were evaluated using serum s les from sheep infected with M. avium subsp. paratuberculosis , and 29 (58%) were found to be antigenic. Among 50 fusion proteins, 10 were evaluated in MBP fusion and factor Xa-cleaved forms. A total of 31 proteins (62%) were found to be antigenic in either MBP fusion or factor Xa-cleaved forms. Antigenicity after cleavage and removal of the MBP tag was marginally enhanced.
Publisher: SAGE Publications
Date: 06-05-2019
Abstract: Johne's disease is an enteric disease caused by the intracellular pathogen
Publisher: Public Library of Science (PLoS)
Date: 23-05-2019
Publisher: American Society for Microbiology
Date: 15-03-2006
DOI: 10.1128/JB.188.6.2290-2293.2006
Abstract: Microarray-based comparisons of three Mycobacterium avium subsp. paratuberculosis isolates, including one sheep strain and two cattle strains, identified three large genomic deletions in the sheep strain, totaling 29,208 bp and involving 24 open reading frames. These deletions may help explain some of the differences in pathogenicity and host specificity observed between the cattle and sheep strains of Mycobacterium avium subsp. paratuberculosis .
Location: United States of America
No related grants have been discovered for John Bannantine.